• Title/Summary/Keyword: T/T probe

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Implementation of a self-mixing type LDF probe and blood flow simulator (자기혼합형 LDF 프로브와 혈류 시뮬레이터의 구현)

  • Ko, Han-Woo;Kim, Jong-Weon
    • Journal of Sensor Science and Technology
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    • v.8 no.2
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    • pp.133-138
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    • 1999
  • In this pager, the authors have implemented a blood flow simulator and a LDF(laser Doppler flowmeter) probe using self-mixing effect of the laser diode. The purpose of the blood flow simulator is to simulate microvascular blood flow in tissue. It consists of melinex film (thickness = $123\;{\mu}m$) which has similar optical characteristics to epidermis and porous polyethylene filter (Vyon, porosity 35%, mean pore size $50\;{\mu}m$, thickness=1 mm) which has similar optical characteristics to dermis. The blood flowmeter probe consists of laser diode(5 mW, 780 nm wavelength), CD lens(focal length 12 mm). current-to-voltage converter, highpass filter, and premplifier. It doesn't need optical fiber, therefore, implementation of the probe is simpler than conventional probe using optical fiber.

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Home-built Solid-state NMR Probe for Membrane Protein Studies

  • Kim, Yong-Ae;Hwang, Jung-Hyun;Park, Jae-Joon
    • Bulletin of the Korean Chemical Society
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    • v.24 no.9
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    • pp.1281-1283
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    • 2003
  • Proteins in highly oriented lipid bilayer samples are useful to study membrane protein structure determination. Planar lipid bilayers aligned and supported on glass slide were prepared. These stack of glass slide with planar lipid bilayers are not well fit for commercial solid-state NMR probe with round coil. Therefore, homebuilt solid-state NMR probe was built and used for a stack of thin glass plates and RF coil is wrapping directly around the flat square sample. The overall filling factor of the coil is much better and the large surface area enhances the extent to orientation by providing uniform environments for the phospholipids and the high ratio of circumference to area reduces edge effects. $^1H\;and\;^{15}N$ double resonance probe for 400 MHz NMR (9.4T) with a flat coil (coil size: 11 mm ${\times}$ 20 mm ${\times}$ 4 mm) is constructed and tested.

The comparison of critical currents measured by hall probe and transport methods for HTS coated conductor

  • Lee, Nam-Jin;Oh, Sang-Soo;Kim, Ho-Sup;Ha, Dong-Woo;Ha, Hong-Soo;Ko, Rock-Kil;Kim, Tae-Hyung;Kim, Hae-Jong;Song, Kyu-Jeong;Moon, Seung-Hyun;Youm, Do-Jun
    • Progress in Superconductivity and Cryogenics
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    • v.11 no.2
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    • pp.11-14
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    • 2009
  • We estimated the critical current distribution of the long length CCs using hall probe method, and checked the reliability by comparing with the four-probe dc transport method. The deviation ratio of $I_{c-T}$ (a measured critical current by four probe dc transport method) and $I_{c-H}$ (calculated critical current by formula (3).) was nearly smaller than 12%. The Hall probe method appeared powerful to evaluate the critical current of CCs in a reel to reel system.

A Study on Algorithm for Travel Time Estimation using Restricted GPS Data (제한된 GPS정보를 활용한 통행 시간 추정 알고리즘에 관한 연구)

  • Yoo, Nam-Hyun
    • The Journal of the Korea institute of electronic communication sciences
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    • v.9 no.12
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    • pp.1373-1380
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    • 2014
  • In order to calculate accurate traffic and traffic speed, qualified and sufficient GPS data should be provided. However, it is difficult to provide accurate traffic information using restricted GPS data from probe vehicles because of communication costs. This paper developed a algorithm that recovers links omitted by restricted GPS data with topology information, and calculate traffic speed with original links and recovered links. T traffic information service of city with a new algorithm can provide more accurate traffic and traffic speed than the original system.

Design of Broad Bandwidth Dipole Antenna with CMM Feeding Probe Structure (CMM 급전 구조를 가지는 광대역 다이폴 안테나의 설계)

  • Lee, Ji-Chul;Min, Kyeong-Sik
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.21 no.9
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    • pp.1030-1036
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    • 2010
  • This paper proposes a simulation design for feeding probe with the CMM(Cylindrical Magneto Material) structure for broad bandwidth of a dipole type antenna. In order to ensure low frequency bandwidth, the magnetic material with high relative permeability was considered in design calculation. It was confirmed that the broad bandwidth was independent on the relative permeability value and depended on the control of distance($r_m$) between magnetic material and feeding probe, and of magnetic material thickness($t_m$). Furthermore, an antenna size with the CMM was miniaturized about 18 % comparison with its size without the CMM.

Rapid detection of Theileria sergenti by the polymerase chain reaction in Korean cattle (중합효소연쇄반응을 이용한 한우에 감염된 Theileria sergenti의 신속한 검출)

  • Chae, Joon-seok;Lee, Joo-mook;Kwon, Oh-deog;Park, Jin-ho;Chae, Keon-sang
    • Korean Journal of Veterinary Research
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    • v.36 no.1
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    • pp.195-207
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    • 1996
  • To make the genomic DNA probe of Theileria sergenti, the merozoites were purified from erythrocytes of Korean cattle, The previous studies on the probe of T sergenti had resulted in two probes as KTS1 and KTS3 DNA fragment. Nucleotide sequence of both ends of the KTS1 and KST3 were determined in order to design primers for polymerase chain reaction. A pair of an uper primer(5'-CCTCTTGAAGTCATCCATGT-3'; nucleotide position 48) and a lower primer(5'-CACTGAGCTG GAAAGAGCTA-3'; nucleotide position 156) in pKTS1 were synthesized. The anticipated PCR product was 128bp in length. To examine the sensitivity of the PCR, KTS1 DNA and purified T sergenti DNA were serially diluted by tenfolds with distilled water. The primers were sensitive enough to detect 4ag of the authentic template DNA and 4fg of the purified T sergenti DNA by PCR. Furthermore, when the blood was serially diluted by two-folds with 0.9% saline, the pair could detect up to 0.00029%(about 164 parasites in $10{\mu}l$ of blood) of T sergenti infection in bovine erythrocytes by PCR. In a comparison of microscopic and PCR detection of T sergenti in the same samples from Chonbuk area, 47 and 51 out of 70 sample(67.1%) were positive by the former and by the latter method, respectively.

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Comparative Dynamics of $tRNA^{val}$ and pBluescript II SK(+) Phagemid Studied with Ethidium Bromide and a Long-lifetime Metal-ligand Complex

  • Kang, Jung-Sook;Yoon, Ji-Hye
    • Journal of Photoscience
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    • v.11 no.3
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    • pp.133-139
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    • 2004
  • The metal-ligand complex, $[Ru(phen)_2(dppz)]^{2+}$ (phen=1,10-phenanthroline, dppz=dipyrido[3,2-a:2',3'-c]phenazine) (RuPD), was used as a spectroscopic probe for studying nucleic acid dynamics. The RuPD complex displays a long lifetime and a molecular light switch property upon DNA binding due to shielding of its dppz ligand from water. To show the usefulness of this luminophore (RuPD) for probing nucleic acid dynamics, we compared its intensity and anisotropy decays when intercalated into the $tRNA^{val}$ and pBluescript (pBS) II SK(+) phagemid through a comparison with ethidium bromide (EB), a conventional nucleic acid probe. We used frequency-domain fluorometry with a blue light-emitting diode (LED) as the modulated light source. The mean lifetime for the $tRNA^{val}$ (<${\tau}$> = 166.5 ns) was much shorter than that for the pBS II SK(+) phagemid (<${\tau}$> = 481.3 ns), suggesting a much more efficient shielding from water by the phagemid. Because of their size difference, the anisotropy decay data showed a much shorter rotational correlation times for the $tRNA^{val}$ (99.9 and 23.6 ns) than for the pBS II SK(+) phagemid (968.7 and 39.5 ns). These results indicate that RuPD can be useful for studying nucleic acid dynamics.

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Effect of RFLP Marker of the Mitochondrial DNA D-Loop Region on Milk Production in Korean Cattle (한우 Mitochondrial DNA D-Loop 영역의 RFLP Marker가 산유량에 미치는 영향)

  • Chung Eui-Ryong;Chung Ku-Young
    • Food Science of Animal Resources
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    • v.25 no.2
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    • pp.218-225
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    • 2005
  • This study was performed to determine sequence variation and RFLP of the mt DNA D-loop region using Southern blot hybridization analysis and to develop mt DNA marker affecting milk production traits in Hanwoo cows. The PCR was used to amplify an 1142 bp fragment within the D-loop region of mt DNA using specific primers. Mt DNA were digested with seven restriction enzymes and hybridized using DIG-labeled D-loop probe. The mt DNA RFLP polymorphisms were observed in the four enzymes, BamHI, RsaI, XbaI and HpaII. Nucleotide substitutions were detected at positions 441 (G/C), 469 (T/C), 503 (C/T), 569 (G/A), 614 (C/A) and 644 (C/T) of the mt DNA D-loop region between two selected lines. Significant relationship between the XbaI RFLP type and breeding value was found(p<0.05). Cows with A type had higher estimated breeding values than those with B type (P<0.05) between high and low milk production lines. Therefore, the RFLP marker of mt DNA could be used as a selection assisted tool for individuals with high milk producing ability in Hanwoo.