• Title/Summary/Keyword: T&E

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Site-Specific Mutagenesis on the 32-T and 39-T of E. coli $tRNA^{phe}$ Gene (E. coli $tRNA^{phe}$ 유전자의 32-T와 39-T 염기의 부의 특이적 돌연변이)

  • 김익영;이세영
    • Korean Journal of Microbiology
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    • v.27 no.3
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    • pp.176-180
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    • 1989
  • There are three pseudourdine ($Psi$)bases in the E. coli $tRNA^{phe}$ In order to study the function of the pseudouridine bases in the $tRNA^{phe}$, changes of bases $tRNA^{phe}$ gene to other bases were undertaken by the site-specific mutagenesis. Site-specific mutagenesis of T in the pheW gene, a $tRNA^{phe}$ gene of E. coli, corresponding to the baseat the No.32 position to C and also T corresponding to the base at the No.39 position to C were performed using Kunkel's uracil-containing template method. Identification of mutants were undertaken by the KNA sequencing techniques of the mutated pheW genes and activities of the mutated pheW genes complementing to E. coli NP37 mutant($pheS^{-ts}$) using the recombinant plasmid containing the mutated genes. Neither NP37 harboring pheW gene mutated at No.32 position nor NP37 harboring pheW gene mutated at No.39 position can be grown at non-permissive temperature. The result means that both mutated pheW genes can not complement to E. coli NP37, and that the pseudouridine bases are essential to the activity of the E. coli $tRNA^{phe}$ in vivo.

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Nonlinear semigroups on locally convex spaces

  • Hyeon, Son-Kuk
    • East Asian mathematical journal
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    • v.6 no.1
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    • pp.111-121
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    • 1990
  • Let E be a locally convex Hausdorff space and let $\Gamma$ be a calibration for E. In this note we proved that if E is sequentially complete and a multi-vaiued operaturA in E is $\Gamma$-accretive such that $D(A){\subset}Re$ (I+$\lambda$A) for all sufficiently small positive $\lambda$, then A generates a nonlinear $\Gamma$-contraction semiproup {T(t) ; t>0}. We also proved that if E is complete, $Gamma$ is a dually uniformly convex calibration, and an operator A is m-$\Gamma$-accretive, then the initial value problem $$\{{\frac{d}{dt}u(t)+Au(t)\;\ni\;0,\;t >0,\atop u(0)=x}\.$$ has a solution $u:[0,\infty){\rightarrow}E$ given by $u(t)=T(t)x={lim}\limit_{n\rightarrow\infty}(I+\frac{t}{n}A)^{-n}x$ each $x{\varepsilon}D(A)$.

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LIE IDEALS IN THE UPPER TRIANGULAR OPERATOR ALGEBRA ALG𝓛

  • LEE, SANG KI;KANG, JOO HO
    • Journal of applied mathematics & informatics
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    • v.36 no.3_4
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    • pp.237-244
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    • 2018
  • Let ${\mathcal{H}}$ be an infinite dimensional separable Hilbert space with a fixed orthonormal base $\{e_1,e_2,{\cdots}\}$. Let L be the subspace lattice generated by the subspaces $\{[e_1],[e_1,e_2],[e_1,e_2,e_3],{\cdots}\}$ and let $Alg{\mathcal{L}}$ be the algebra of bounded operators which leave invariant all projections in ${\mathcal{L}}$. Let p and q be natural numbers (p < q). Let ${\mathcal{A}}$ be a linear manifold in $Alg{\mathcal{L}}$ such that $T_{(p,q)}=0$ for all T in ${\mathcal{A}}$. If ${\mathcal{A}}$ is a Lie ideal, then $T_{(p,p)}=T_{(p+1,p+1)}={\cdots}=T_{(q,q)}$ and $T_{(i,j)}=0$, $p{\eqslantless}i{\eqslantless}q$ and i < $j{\eqslantless}q$ for all T in ${\mathcal{A}}$.

Development of KEPCO e-IoT Standard Type oneM2M Gateway for Efficient Management of Energy Facilities (에너지 설비의 효율적 관리를 위한 한전 e-IoT 표준형 oneM2M Gateway 개발)

  • Sim, Hyun;Kim, Yo-Han
    • The Journal of the Korea institute of electronic communication sciences
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    • v.16 no.6
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    • pp.1213-1222
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    • 2021
  • This study is a digitalization study based on ICT technology as part of the development of innovative technologies in the new energy industry as a 2050 carbon-neutral policy. It is the development of an oneM2M-based IoT server platform that can be integrated and managed in conjunction with the external interface of each energy facility. It analyzes KEPCO's e-IoT standard specifications through the Power Research Institute's 'SPIN' and develops representative standards, LWM2M and oneM gateway platforms. OneM2M secures and analyzes the recently announced standard for Release 2 instead of the existing Release 1. In addition, the e-IoT standard oneM2M platform is developed based on R2. In addition, it selects the specifications for e-IoT gateway devices that can sufficiently implement KEPCO's e-IoT standards. In addition, a technology and system for developing a high-performance gateway device that considers future scalability were proposed.

A NOTE ON BITRANSFORMATION GROUPS

  • Song, Hyung Soo
    • Korean Journal of Mathematics
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    • v.14 no.2
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    • pp.227-232
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    • 2006
  • We study some dynamical properties in the context of bitransformation groups, and show that if (H,X,T) is a bitransformation group such that (H,X) is almost periodic and (X/H,T) is pointwise almost periodic $T_2$ and $x{\in}X$, then $E_x=\{q{\in}E(H,X){\mid}qx{\in}{\overline{xT}\}$ is a compact $T_2$ topological group and $E_{qx}=E_x(q{\in}E(H,X))$ when H is abelian, where E(H,X) is the enveloping semigroup of the transformation group (H,X).

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A Research for the Development of the Transmission Error Measuring System and Transmission Error Specification for the Gear Noise Quality (기어 소음 품질 안정화를 위한 전달오차 측정 시스템 개발 및 전달오차 스펙 개발에 대한 연구)

  • Lee, Sang Hwa;Lee, Hyun Ku;Kang, Seok Chan;Kim, Si Yoong;Yoo, Dong Kyu;Won, Kwang Min;Chae, Geum Muk;Lee, Tae Hwi
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.24 no.6
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    • pp.470-475
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    • 2014
  • In the past, transmission error(T.E.) has been identified as one of the main sources of gear whine noise, which cause serious passengers irritation at high frequency. In this study, to identify the correlation of the T.E. and gear whine noise, a T.E. measuring system was developed. By comparing the T.E. and gear whine noise level, T.E. target value which can represent the gear noise was proposed. The conclusions are followed: (I) It is possible to measure T.E. of the gear pairs (II) Similar trends are observed between T.E. and gear whine noise. (III) By observing the T.E. pattern, presence of sideband noise can be predicted. (IV) Proposed T.E. target value can be adopted to the gear manufacturing for gear noise quality management.

A Phytosociological Study of Hokkaido Vegetation, Japan (북해도 식생에 대한 식물사회학적 고찰)

  • Kim, Jong-Won
    • The Korean Journal of Ecology
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    • v.12 no.2
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    • pp.109-122
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    • 1989
  • The vegetation and landscape of Hokkaido were phytosociologically referred. The region of F a g e t e a c r e n a t a e on Hokkaido is divided into two types of deciduous broad-leaved forest: the oak (Quercus mongolica var. grosseserrata) forests mixed with conifers (mainly Abies sachalinensis) and the beech (Fagus crenata) forests of northernmost distribution in far-east Asia. The oak forests, which is dominated by Quercus mongolica var. grosseserrata in Japanse islands, seem to be developed from different climatic and edaphic conditions, especially in the amount and sharing pattern of precipitation in a year, and weak acid brown forest soil, volcanic ash soil and sandy soil. On the all-inclusive phytogeographical view-point, Hokkaido is situated at northernmost region of F a g e t e a c r e n a t a e (cool-temperate zone)neighboring with subarctic and subalpine vegetation, vegetation, but the evergreen broad-leaved forest (C a m e l l i e t e a j a p o n i c a e, warm-temperate zone) is abscent.

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Age and Growth of Black Rockfish, Sebastes schlegeli in the Tongyeong Marine Ranching Area in Korea Waters (통영바다목장에 서식하는 조피볼락, Sebastes schlegeli의 연령과 성장)

  • Park, Kyeong Dong;Kang, Yong Joo
    • Korean Journal of Ichthyology
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    • v.19 no.1
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    • pp.35-43
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    • 2007
  • Age and growth of black rockfish, Sebastes schlegeli sampled from the Tongyeong marine ranching area in Korea waters were determined from 1,173 otoliths from July, 2001 to May, 2004. Examination of outer margins of the otolith showed that the opaque zone was formed once a year. Marginal increment of the otolith formed annual ring from July. The von Bertalanffy growth curve had the growth parameters estimated from non-linear regression were $L_t=48.45(1-e^{-0.2139(t+0.4313)})$, $W_t=1,837.93(1-e^{-0.2139(t+0.4313)})^{3.02}$ 3.02 for females and, $L_t=49.32(1-e^{-0.1775(t+0.7403)})$, $W_t=1,887.83(1-e^{-0.1775(t+0.7403)})^3$ for males, where t is age (year) and Lt is body length (mm) at age t. Growth at the age of male and female shows significant difference (p<0.01).

Genome-Based Reclassification of Strain KIST612, Previously Classified as Eubacterium limosum, into a New Strain of Eubacterium callanderi

  • Ji-Yeon Kim;Byeongchan Kang;Soyoung Oh;Yeji Gil;In-Geol Choi;In Seop Chang
    • Journal of Microbiology and Biotechnology
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    • v.33 no.8
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    • pp.1084-1090
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    • 2023
  • The strain KIST612, initially identified as E. limosum, was a suspected member of E. callanderi due to differences in phenotype, genotype, and average nucleotide identity (ANI). Here, we found that E. limosum ATCC 8486T and KIST612 are genetically different in their central metabolic pathways, such as that of carbon metabolism. Although 16S rDNA sequencing of KIST612 revealed high identity with E. limosum ATCC 8486T (99.2%) and E. callanderi DSM 3662T (99.8%), phylogenetic analysis of housekeeping genes and genome metrics clearly indicated that KIST612 belongs to E. callanderi. The phylogenies showed that KIST612 is closer to E. callanderi DSM 3662T than to E. limosum ATCC 8486T. The ANI between KIST612 and E. callanderi DSM 3662T was 99.8%, which was above the species cut-off of 96%, Meanwhile, the ANI value with E. limosum ATCC 8486T was not significant, showing only 94.6%. The digital DNA-DNA hybridization (dDDH) results also supported the ANI values. The dDDH between KIST612 and E. callanderi DSM 3662T was 98.4%, whereas between KIST612 and E. limosum ATCC 8486T , it was 57.8%, which is lower than the species cut-off of 70%. Based on these findings, we propose the reclassification of E. limosum KIST612 as E. callanderi KIST612.

Effects on n-Alcohols on the Amino-Proton Chemical Shifts and on the Hindered Rotation about the N-C(O) Bond of Acetamide (아세트아미드의 아미노 양성자의 화학적 이동과 N-C(O) 결합주위의 부자유회전에 미치는 n-알코올 용매 효과)

  • Gwon, Sun Gi;Choe, Jong Ho;Choe, Yeong Sang;Yun, Chang Ju;Gwon, Dae Geun
    • Journal of the Korean Chemical Society
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    • v.34 no.6
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    • pp.509-516
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    • 1990
  • 1H-nmr chemical shifts and lineshapes of amino-protons of acetamide (AA) in n-alcohols were determined. The chemical shifts are discussed by the Reichardt's solvent polarity parameter, E$_{T}$(30). The following relationship between $\delta$obs and E$_{T}$(30) was obtained. ${\delta}_{obs}$ = ${\delta}_{o}$ + aE$_{T}$ (30) + b[E$_{T}$(30)]$^2$ where ${\delta}_{o}$ is the chemical shift of the solute in gaseous state or at $E_{T}$(30) = 0, a is a characteristic constant for the protons of AA in n-alcohol solutions and b is a constant for the solute (AA)-solvent (n-alcohols) interaction. The barrier of the hindered rotation about the N-C(O) bond in AA was obtained by analysis of the lineshapes of the amino-protons in AA. The behavior of the internal rotation as well as chemical shifts of the amino-protons in AA has been found to be closely related to the $E_{T}$(30) of n-alcohols.

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