• Journal of Food Hygiene and Safety
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• v.8 no.1
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• pp.9-15
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• 1993

In an attempt to quantitate and qualitate residual antibiotics and antibacterial agents in meat simultaneously, we studied a gas chromatography-mass spectrometry(GC/M8) analysis. For a simultaneous analysis of penicillin G, chloramphenicol and thiamphenicol in meat, a simple and rapid clean-up procedure including extraction with 0.01 M EDTA-2Na Mcilvaine buffer (pH 4.0), defatting with n-hexane, and elution with 0.01M-methanolic oxalic acid from Bond Elute $C_{18}$ cartridge, and quantitation by selected ion monitoring (SIM) mode after derivatization was performed. The recoveries (%) of penicillin G, chloramphenicol and thiamphenicol (CV, %) at 1 ppm fortification level were 63.5 (7.6), 76.3 (8.1) and 84.7 (2.0), and the detection limits of those were 0.6, 0.085 and $0.084\;\mu\textrm{g}$ beef, respectively. This method using 81M mode allows excellent detection and quantitation of residual antibiotics and antibacterial agents in meat. Moreover, confirmation by a full scan electron impact mass spectrum is possible if residual level in the sample in above 1 ppm.

• Korean Journal of Food Science and Technology
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• v.25 no.2
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• pp.148-153
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• 1993

Composition and structure of synthetic glucitol fatty acid polyesters (GPE)-a potential fat substitute-were investigated. Also degree of substitution (D.S) of GPE was determined according to the relative ester distribution within it to evaluate the feasibility of GPE using as a fat substitute. The GPE was separated into single ester group by a normal-phase HPLC and D.S of it was identified to be 6. Absorption band at $1747\;cm^{-1}$ in the IR spectrum of GPE indicated that there were ester bonds within GPE molecules. which link fatty acid moiety to glucitol. Disappearance of the hydroxyl proton signals of glucitol in the H-NMR spectrum of GPE implied that most of hydroxyl groups in glucitol participated in the formation of ester bonds with fatty acids. In addition the D.S estimated from the quantitative proton integration of GPE coincided well with the D.S of GPE determined by hydroxyl value measurement. In conclusion, the GPE synthesized in this study was found to be a glucitol fatty acid hexaester so that it is expected to be used as a fat substitute in the near future.

• Journal of Biomedical Engineering Research
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• v.25 no.6
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• pp.581-587
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• 2004

Conventional power spectrum methods based on FFT, AR method are not appropriate for analyzing biomedical signals whose spectral characteristics change rapidly. On the other hand, time-frequency analysis has more desirable characteristics of a time-varying spectrum. In this study, we investigated the spectral components of heart rate variability(HRV) in time-frequency domain using time frequency analysis methods. In the various time-frequency kernels functions, we studied the suitable kernels for the analysis of HRV using synthetic HRV signals. First, we evaluated the time/frequency resolution and cross term reduction of various kernel functions. Then, from the instantaneous frequency, obtained from time-frequency distribution, the method extracting frequency components of HRV was proposed. Subjects were 17 healthy young men. A coin-stacking task was used to induce mental stress. For each subjects, the experiment time was 3 minutes. Electrocardiogram, measured during the experiment, was analyzed after converted to HRV signal. In the results, emotional stress of subjects produced an increase in sympathetic activity. Sympathetic activation was responsible for the significant increase in the LF/HF ratio. Subjects were divided into two groups with task ability. Subjects who have higher mental stress have lack of task ability.

• Geophysics and Geophysical Exploration
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• v.15 no.2
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• pp.51-56
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• 2012

One of the most critical and essential procedures in the interpretation of gravity and magnetic data is to separate the anomaly due to the specific geologic structure from the summation of effects from a broad variety of geologic sources, especially those of different depths. Separation of the residual anomaly from the regional field is the most simple case of the vertical separation. If the anomaly due to a layer of specific depth can be separated or the depth of the separated layer can be quantitatively determined, it may deserve the separation-sounding. We suggest a wavelength filter whose cutoff frequency is determined by log-power spectrum analysis, as a separation-sounding filter. We applied this filter both to synthetic and real gravity data acquired at Heunghae area, and compared the results with those of Jacobsen's upward continuation filter. These showed that the proposed separation-sounding filter could be a useful tool for interpretation of the vertical geologic structure by stripping the gravity effects of geologic sources down to the desired depth.

• Korean Journal of Veterinary Research
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• v.37 no.4
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• pp.855-862
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• 1997

Ivermectin is a synthetic derivative of the naturally occurring avermectin $B_{1a}$ (22, 23-dihydroavermectin $B_{1a}$) and $B_{1b}$ (22, 23-dihydroavermectin $B_{1b}$), It is widely used as antiparasitic and pesticidal agents because of its remarkably potent and broad spectrum of antiparasitic activity. Although the drug has shown excellent anthelmintic effects, development of toxicosis in some animals such as the Collie species of dog is well documented. However, no studies have been reported on the toxic effects of the drug in Korean native animals such as the Jindo dog. The toxic effect of ivermectin was evaluated in 25 Jindo dogs divided into five groups which were orally administered with ivermectin at dosage levels of $200{\mu}g/kg$, $300{\mu}g/kg$, $600{\mu}g/kg$ and $2,500{\mu}g/kg$ of body weight, respectively. Toxic signs were not observed in the groups receiving $200{\mu}g/kg$ and $300{\mu}g/kg$ B.W. ivermectin. One dog developed mild clinical signs of toxicosis in the group receiving $600{\mu}g/kg$ dosage of ivermectin. In the group with $2,500{\mu}g/kg$ dosage, all dogs developed mild (salivation, drooling, vomiting, mydriasis, and/or confusion) and/or moderate (ataxia and tremors) clinical signs of toxicosis. Hematologic changes were not observed in the groups receiving $200{\mu}g/kg$, $300{\mu}g/kg$ and $600{\mu}g/kg$ B.W. ivermectin. In the groups receiving $2,500{\mu}g/kg$ B.W., total erythrocyte counts, total and differential leukocyte counts and hemoglobin levels were not affected by drug. Aspartate aminotransferase levels were increased after administration of ivermectin, while serum cholesterol and blood glucose levels were decreased.

• Journal of Microbiology and Biotechnology
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• v.27 no.6
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• pp.1120-1127
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• 2017

Marasmius scorodonius secretes an extracellular laccase in potato dextrose broth, and this enzyme was purified up to 206-fold using $(NH_4)_2SO_4$ precipitation and a Hi-trap Q Sepharose column. The molecular mass of the purified laccase was estimated to be ~67 kDa by SDS-PAGE. The UV/vis spectrum of the enzyme was nontypical for laccases, and metal content analysis revealed that the enzyme contains 1 mole of Fe and Zn and 2 moles of Cu per mole of protein. The optimal pH for the enzymatic activity was 3.4, 4.0, and 4.6 with 2,2'-azino-bis(3-ethylbenzothazoline-6-sulfonate) (ABTS), guaiacol, and 2,6-dimethoxy phenol as the substrate, respectively. The optimal temperature of the enzyme was $75^{\circ}C$ with ABTS as the substrate. The enzyme was stable in the presence of some metal ions such as $Ca^{2+}$, $Cu^{2+}$, $Ni^{2+}$, $Mg^{2+}$, $Mn^{2+}$, $Ba^{2+}$, $Co^{2+}$, and $Zn^{2+}$ at a low concentration (1 mM), whereas $Fe^{2+}$ completely inhibited the enzymatic activity. The enzymatic reaction was strongly inhibited by metal chelators and thiol compounds except for EDTA. This enzyme directly decolorized Congo red, Malachite green, Crystal violet, and Methylene green dyes at various decolorization rates of 63-90%. In the presence of 1-hydroxybenzotriazole as a redox mediator, the decolorization of Reactive orange 16 and Remazol brilliant blue R was also achieved.

• Geophysics and Geophysical Exploration
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• v.16 no.4
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• pp.225-233
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• 2013

The accuracy of the automatic NMO velocity analysis, which is used for an effective and objective NMO velocity analysis, is highly affected by the velocity resolution of the velocity spectrum. In this study, we have developed an automatic NMO velocity algorithm, where the velocity spectra are created using high-resolution bootstrapped differential semblance (BDS), and the velocity analysis on CMP gathers is performed in parallel with MPI. We also compared the velocity models from the developed automatic NMO velocity algorithm with high-resolution BDS to those from BDS. To verify the developed automatic velocity analysis module we created synthetic seismic data from a velocity model including horizon layers. We confirmed that the developed automatic velocity analysis module estimated velocity more accurately. In addition, NMO velocity which yielded a CMP stacked section, where the coherency of the events were improved, was estimated when the developed module was applied to a marine field data set.

• Korean Journal of Materials Research
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• v.26 no.9
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• pp.504-511
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• 2016

To prepare $Mn^{4+}$-activated $K_2TiF_6$ phosphor, a precipitation method without using hydrofluoric acid (HF) was designed. In the synthetic reaction, to prevent the decomposition of $K_2MnF_6$, which is used as a source of $Mn^{4+}$ activator, $NH_5F_2$ solution was adopted in place of the HF solution. Single phase $K_2TiF_6$:$Mn^{4+}$ phosphors were successfully synthesized through the designed reaction at room temperature. To acquire high luminance of the phosphor, the reaction conditions such as the type and concentration of the reactants were optimized. Also, the optimum content of $Mn^{4+}$ activator was evaluator based on the emission intensity. Photoluminescence properties such as excitation and emission spectrum, decay curve, and temperature dependence of PL intensity were investigated. In order to examine the applicability of this material to a white LED, the electroluminescence property of a pc-WLED fabricated by combining the $K_2TiF_6$:$Mn^{4+}$ phosphor with a 450 nm blue-LED chip was measured.

• Earthquakes and Structures
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• v.6 no.4
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• pp.375-392
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• 2014

Time history analyses have been preferred commonly in earthquake engineering area to determine earthquake performances of structures in recent years. Advances in computer technology and structural analysis have led to common usage of time history analyses. Eurocode 8 allows the use of real earthquake records as an input for linear and nonlinear time history analyses of structures. However, real earthquake records with the desired characteristics sometimes may not be found, for example depending on soil classes, in this case artificial and synthetic earthquake records can be used for seismic analyses rather than real records. Selected earthquake records should be scaled to a code design spectrum to reduce record to record variability in structural responses of considered structures. So, scaling of earthquake records is one of the most important procedures of time history analyses. In this paper, four real earthquake records are scaled to Eurocode 8 design spectrums by using SESCAP (Selection and Scaling Program) based on time domain scaling method and developed by using MATLAB, GUI software, and then scaled and real earthquake records are used for linear time history analyses of a six-storied building. This building is modeled as spatial by SAP2000 software. The objectives of this study are to put basic procedures and criteria of selecting and scaling earthquake records in a nutshell, and to compare the effects of scaled earthquake records on structural response with the effects of real earthquake records on structural response in terms of record to record variability of structural response. Seismic analysis results of building show that record to record variability of structural response caused by scaled earthquake records are fewer than ones caused by real earthquake records.

• Publications of The Korean Astronomical Society
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• v.14 no.1
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• pp.33-37
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• 1999

We have constructed a synthetic spectrum of the 2.5 micron $C_2H$ bands and compared them with diminutive structures in the near-infrared spectra of Comets P/Halley and West (1976 VI). We found that the Q branches of the $C_2H$ bands coincide with two small emission peaks in the spectra of the comets. We undertook Monte Carlo simulations using observed emission intensities of $C_2$ and possibly $C_2H$ in Comet P/Halley in order to derive a lifetime range of $C_2H$ and a production rate at the time of observations of P/Halley. We obtained a $C_2H$ production rate of $1\times10^{27}\;sec^{-1}$ for P/Halley on December 20, 1985, assuming the 2.5 micron features are due to $C_2H$. We derived a very short lifetime (<100 seconds) of $C_2H$ at 1AU heliocentric distance, assuming that the only parent molecule for $C_2H$ and $C_2$ is $C_2H$. Using this short lifetime we were unable to fit our $C_2$ distribution model to $C_2$ distribution curves observed by O'Dell et al.(1988), because our curve shows a steep slope compared with the observed one. We conclude that there must be significant additional source(s) for $C_2H$ and $C_2$ other than $C_2H_2$.