• Bulletin of the Korean Chemical Society
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• v.32 no.7
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• pp.2253-2259
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• 2011

The self-assembly of one novel organic complex based on chlorogenic acid (HCA) and 2,2'-bipyridine (2,2'-bipy) has been synthesized and characterized. The complex achieved by hydrogen-bonding interactions, adopted a 1:1 stoichiometry in a solid state. The proton transfer occurred from the carboxyl oxygen to the aromatic nitrogen atom to form salts CA${\cdot}$(2,2'-Hbipy), the 2,2'-Hbipy molecule individually occupies the pseudo-tetragonum that is formed with CA. In this paper, the interactions of CA${\cdot}$(2,2'-Hbipy) with bovine serum albumin (BSA) were studied by fluorescence spectrometry. For CA${\cdot}$(2,2'-Hbipy), HCA and 2,2'-bipy, the average quenching constants for BSA were $2.4384{\times}10^4$, $4.653{\times}10^3$, and $3.059{\times}10^3\;L{\cdot}mol^{-1}$, respectively. The mechanism for protein fluorescence quenching is apparently governed by a static quenching process. The Stern-Volmer quenching constants and corresponding thermodynamic parameters ${\Delta}$H, ${\Delta}$G and ${\Delta}$S were calculated. The binding constants and the number of binding sites were also investigated. The conformational changes of BSA were observed from synchronous fluorescence spectra.

• Journal of Korean Society on Water Environment
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• v.25 no.4
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• pp.560-567
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• 2009

Treated or untreated wastewater may be a major source of refractory organic matters (R-OM) in drinking water sources. For this study, spectroscopic characteristics of wastewater OM were investigated using the samples from 20 wastewater treatment plants, which are located at the upstream of the lake Paldang, to suggest a estimate index for R-OM in wastewater. R-OM was quantified by measuring total organic carbon (TOC) concentration of the wastewater samples remaining after 28-day dark incubation. Among the traditional OM indices such as chemical oxygen demand (COD) and initial TOC, CODMn showed the lowest correlation coefficients with R-TOC of the samples. The ratios of carbonaceous biochemical oxygen demand (CBOD) to $COD_{Cr}$ had a better correlation with the percent distribution of R-OM than $BOD/COD_{Cr}$ ratios. terrestrial humic-like fluorescence (THLF) exhibited the highest correlation coefficient with R-TOC among the indices obtained from the synchronous fluorescence spectra of the samples. Milori index, one of the humification indices, showed a good correlation with the percent distribution of wastewater. This study demonstrated that fluorescence properties might be a better indices to estimate the concentrations and the distributions of wastewater OM compared to the specific UV absorbance (SUVA) values. Some useful formulas based on OM spectroscopic characteristics were finally suggested to predict R-OM in wastewater.

• Journal of Korean Society on Water Environment
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• v.25 no.3
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• pp.419-424
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• 2009

Characteristics of humic substances on the changes in dissolved organic matter (DOM) characteristics by biodegradation was investigated using three types of the artificial water samples composed of glucose and Suwannee River fulvic acid (SRFA). Some selected DOM characteristics including the specific UV absorbance (SUVA), the synchronous fluorescence spectra and the molecular weight (MW) were compared for the artificial water samples before and after 28-day microbial incubation. The changes of the DOM characteristics were minimal for SRFA during the incubation whereas they were significant for glucose. SUVA, dissolved organic carbon (DOC)-normalized fluorescence intensity, and MW values of glucose increased, suggesting that such labile organic compounds could be exclusively transformed into more humidified materials by biodegradation. For glucose-SRFA mixture, the selected DOM characteristics were greater than those estimated using the assumption that the individual changes of either glucose or SRFA are conservative for the mixture of the two materials. Our results suggest that the presence of humic substances (HS) may lead to the enhancement of the formation of refractory organic materials during biodegradation of labile compounds. Detailed analyses of size exclusion chromatography (SEC) revealed that the enhancement occurred for the DOM mixture with a MW range between 500 Da to 4000 Da.

• Journal of Korean Society on Water Environment
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• v.25 no.4
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• pp.589-596
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• 2009

The spectroscopic characteristics and chemical oxygen demand (COD) oxidation efficiencies were investigated for dissolved organic matters (DOM) from diverse sources, which may indirectly affect the concentrations and the quality of DOM found in watersheds. The DOM investigated for this study showed a wide range of the percent distributions of refractory organic matter (R-OM) from 8 to 100%. Relatively high R-OM distributions were observed for the DOM with the source of head water, sediments, paddy soils, field soils, and treated sewage whereas the DOM from livestock waste, reed, weeds, algae, and attached algae exhibited lower R-OM percent distributions. The percent distribution of R-OM had positive correlations with specific UV absorbance (SUVA) and humidification indices (HIX) of DOM. The investigated DOM was classified into four different source groups (i.e., biota, vegetables, soils, sediments) by comparing the synchronous fluorescence spectra. The DOM group from biota source was characterized by a prominent presence of protein-like fluorescence (PLF) whereas fulvic-like fluorescence (FLF) was additionally observed for vegetable-source DOM. FLF became significant for the DOM from both soils and sediments although no PLF was found for soil-derived DOM. A range of COD oxidation efficiency was observed for the various DOM, ranging from 36 to 94% and from 65 to 125% for $COD_{Mn}$ and $COD_{Cr}$, respectively. The results indicate that $COD_{Cr}$ reflects the higher OM concentration than $COD_{Mn}$. However, 95% confidence intervals of the COD oxidation efficiencies were similar for the two types of COD, suggesting that $COD_{Cr}$ may not be the superior OM index to $COD_{Mn}$ in terms of the variability of the oxidation efficiency. No significant correlations were obtained between COD oxidation efficiencies and the spectroscopic characteristics of DOM for this study.

• Analytical Science and Technology
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• v.15 no.4
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• pp.373-380
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• 2002

Humic and fulvic acids present in soil of different depth were extracted and their characteristics were analyzed as a basic study to evaluate the effect of humic substances on the behaviour of radioactive elements deposited on soil. Molecular size distribution of the humic and fulvic acids was measured by stirred cell ultrafiltration technique and the structural informations were obtained from their UV-Vis., IR and synchronous fluorescence (SyF) spectral analysis. Main molecular size ranges of the soil 1) humic and fulvic acids were 30~100 kDa (46~56%) and 10~30 kDa (33~43%) respectively, and their overall molecular sizes were found to became smaller with increasing the soil depth. Absorptivities measured at 280 nm in the UV-Visible spectra of humic acids were 1.4~1.5 times higher than those of fulvic acids, and increased with increasing the soil depth. SyF spectral data showed two distinct peak components having maximum peak positions of 428 nm (type I) and 498 nm (type II) for the soil humic and fulvic acids. From the analysis of the peak components, it was found that humic molecules are mainly made up of aromatic compounds corresponding to longer wavelength (type II), and the molecular components increased with increasing the soil depth. Analysis of IR spectral data indicated that the humic molecules contain a higher relative concentration of carboxylic groups than those of fulvic molecules, and the carboxylic group contents are seen to increase as the soil depth increase.