• Journal of Animal Science and Technology
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• v.62 no.4
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• pp.460-467
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• 2020

The effects of arginine (Arg) and methionine (Met) supplementation on nutrient use in pigs were determined under hot season conditions. A total of five experimental diets including basal diet (CON) were supplemented with two types of amino acids (Arg and Met) and two different amounts of amino acids (0.2% and 0.4%). Under hot season condition, pigs fed with additional Arg were significantly higher in average daily gain (ADG) than the CON group and the ADG increased linearly (p < 0.05) with increasing Arg supplementation. But there was no significant difference with Met supplementation (p > 0.05). The apparent ileal digestibility (AID) of amino acids had no significant difference among treatments (p > 0.05), while d-reactive oxygen metabolites (d-ROMs) concentration in treatments with Arg supplementation, were significantly higher (p < 0.05) than other treatments. In conclusion, exposure of pigs to heat stress does not affect the AID of amino acid, whereas pig fed with additional Arg improved ADG and feed efficiency under heat stress condition.

• Food Science of Animal Resources
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• v.38 no.2
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• pp.302-314
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• 2018

This study investigated the effect of rice bran fiber (RBF) and wheat fibers (WF) on microbiological and physicochemical properties of fermented sausages during ripening and storage. The experimental design included three treatments: Control, no addition; RBF, 1.5%; and WF, 1.5%. During the ripening periods, the addition of dietary fibers rapidly decreased pH and maintained high water activity values of fermented sausages (p<0.05). Lactic acid bacteria were more prevalent in fermented sausages with rice bran fiber than control and sausages with added wheat fiber. During cold storage, lower pH was observed in sausages with dietary fibers (p<0.05), and the water activity and color values were reduced as the storage period lengthened. Fermented sausages containing dietary fibers were higher in lactic acid bacteria counts, volatile basic nitrogen and 2-thiobarbituric acid reactive substance values compared to the control (p<0.05). The results indicate that, the addition of dietary fibers in the fermented sausages promotes the growth of lactic bacteria and fermentation, and suggests that development of functional fermented sausages is possible.

• Korean Journal of Veterinary Service
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• v.14 no.2
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• pp.110-120
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• 1991

28 porcine enteroviruses were isolated from 86 pig-feces of 9 swine farms located in south region, Chung-buk, from March to September 1990. Physico-chemical properties and pathogenicity of isolates were investigated. Results obtained throughout experiments are summarized as follows. According to the age, weanlings(40-90 days), sucklings(10-30 days) and adult pigs(6 months over) showed the isolation rate of 67%. 8% and 4%, respectively. By physico-chemical tests, YD-90/22, YD-90/43 and YD-90/64 strains were found to be ether, chloroform and PH stable. Nucleic acid test suggests the virus to have a DNA genome. Most of the Isolates were not evident of hemagglutinin using erythrocytes from various mammalian & avian. 22 strains among the isolates were shown CPE type I and the remainders were CPE type II. 3 strains among isolates of CPE type I strains were neutralized with high titers to serotype 2 antiserum. In the study on virus growth curve in PK-l5 cells, YD-90/22, YD-90/43 and YD-90/64 strains showed the maximum infectivity titers($10^{6.0}-l0^{6.5} TCID({50}ml$) at 4days post inoculation(PI). When 30 day-old commercial piglets were inoculated only intraoral route with the YD-90/22 strain at $10^{6.0} TCID_{50}ml,$ piglets not showed the symptoms. But piglets inoculated by intramuscle route, intraoral and intramuscle route after pretreat with dexamethasone(2.5mg /kg) for 5 days were shown the symptoms of anorexia, diarrhea, pyrexia and ataxia at 4th-6th days PI. The viral reisolation in the virus-inoculated piglets was examined from feces. The viruses were recovered intermittently from 2nd to 16th day PI and at 4th-6th day PI, all piglets excreted viruses.

• Microbiology and Biotechnology Letters
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• v.6 no.1
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• pp.17-22
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• 1978

The microbial and chemical changes, and characterization of the predominant acid-producing bacteria in the fermenting pig feces blended with corn meal at a ratio of 50：50 were studied. The fermentation was dominated by lactobacilli, which multiplied rapidly for the first 24 hours. The acid produced during the fermentation caused rapid pH drop to pH 4.5 and halted the growth of E. coli and yeast. The initial acid producing bacteria in the mixture was predominantly Streptococcus species, which were reduced in number rapidly. After 7 days of fermentation, three lactobacilli species were appeared L. acidophilus, L. fermenti, L. delbrueckii. Chemical changes during the fermentation were also studied. The lactic acid fermentation imparted a good tangy acid flavor to the corn-feces mixture by removing or covering the .fecal ordour and made the corn-feces mixture palatable for the animal as well as halted the unwanted microbial flora. We hope the lactic acid fermentation will replace the heat processing in the utilization of animal feces.

• Journal of Veterinary Clinics
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• v.16 no.2
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• pp.381-388
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• 1999

These investigations were performed to determine the causes of the reproductive disorders of the sows in a pig farm which was located at the distance 36m away from the detonation spot. The reproductive problems of the sows such as abortion and infertility occurred after consecutive detonation and use of heavy equipments to build 6 piers of the bridge for the expressway between Jinju and Taeieon during the period from July 1997 to October 1998. 1. The management condition of the farm, microorganism in the feed, inbreeding, hematological conditions, and viral agent causing abortion were not proved as the factors of the occurrence of abortion and infertility in the pig farm. 2. Theoretical concussion values according to the average amount of explosive per detonation were 1.16 kine for minimum amount (3.6 kg) and 3.45 kine for maximum amount(12.5 kg) of explosive, and the theoretical sound values were accordingly 92.29 decibel for minimum amount and 101.76 decibel for maximum amount of explosive, respectively, in the case of 36m distance between the detonation spot and pig farm. The frequency of detonation in a day was 7 times with interval of 15 minutes. 3. The occurence of infertility in the farm was 57.3% during the period of construction from July 1997 to August 1998. 4. In comparison of body weight of piglets between the damaged and not damaged farms, growth of piglets at the damaged farm was shown to be delayed over 30 days than those of not-damaged farm. In conclusion, abortion of 40 sows, delivery of whole still births of 22 sows, and infertility of 122 sows in the pig farm were assumed to be caused by the concussion or sound stress after consecutive detonation and use of heavy equipments.

• Journal of Life Science
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• v.19 no.9
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• pp.1304-1308
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• 2009

To investigate the infection patterns of porcine epidemic diarrhea virus (PEDV) in Korean pig farms, a total of 4,768 swine sera samples from 159 pig farms were taken twice, in June (n=82) and October (n=77) in 2007. In each farm selected for the survey, 10 samples from breeding pigs and 4 from each of the 5 age groups (30, 60, 90, 120, and 150 days) were taken, and all serum samples were tested for PEDV by the serum neutralization test. The overall seroprevalence was 62.6% (2,983/4,768), with the highest prevalence in breeding pigs (93.5%, 1,485/1,589). The prevalence showed an increasing trend with increasing age (30.8, 27.2, 44.7, 61.6, and 71.2% respectively in the 30, 60, 90, 120, and 150 days age groups) (p<0.0001 for $x^2$ trend test). The association between age and PEDV prevalence was similar in both surveys, indicating that the infection of PEDV seemed to be occurring repeatedly in the farms surveyed. This inference could also be explained by the fact that prevalence in sows was very high despite low vaccination coverage, as they are continuously exposed to PEDV in potentially infected farms for a longer period. Based on the neutralizing antibody levels in sows and growing pigs, the majority of farms (91.8%, n=146 farms) were endemically infected with PEDV, and most of pigs seemed to be intensively infected with PEDV at around early growth (41.8%) and weaning (31.5%). On the other hand, serum neutralizing antibodies were not detected in pigs older than 30 days of age in farms classified as having no PEDV infection (n=13 farms), indicating the level of maternal antibody against PEDV is decreased on a non-detectable level before the piglet is 60 days old in the field situation. The results indicated that most farms surveyed in 2007 were affected with endemic PEDV infection. Therefore, a national monitoring and control program for the endemic type PEDV infection needs further attention.

• Korean Journal of Veterinary Research
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• v.55 no.2
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• pp.145-148
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• 2015

Four 3-day-old piglets with retarded growth were submitted to the Animal and Plant Quarantine Agency for diagnosis. Necropsy showed that one piglet had black spots ranging from 2 mm to 1 cm in diameter in the cerebellum, lungs, regional lymph nodes, and cecum. Histological findings were consistent with the gross appearance in which melanin pigmentation was observed in the organs mentioned above. Based on Fontana-Masson staining, we diagnosed this animal with systemic melanosis. To the best of our knowledge, this is the first case of systemic melanosis in black breeds of swine in Korea.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.3
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• pp.320-324
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• 2012

The objective of this study was to evaluate the effect of spent mushroom substrate (SMS) derived from Pleurotus eryngii on the hematological and biochemical blood properties of elk. A total of 18, two and three-year-old elk were fed three different levels of SMS (0, 15 and 20%) in a corn-wheat bran diet for 80 days. The results indicated significantly high levels of blood monocytes, hemoglobin (Hb), and hematocrit (HCT) in elk fed 15% or 20% SMS (p<0.05) compared to control animals. Serum blood urea nitrogen (BUN) and glucose concentrations were also significantly elevated in elk fed both 15% and 20% SMS. The inclusion of SMS in the elk diet did not affect serum total cholesterol, triglyceride, or low density lipoprotein (LDL)-cholesterol concentrations; however, high density lipoprotein (HDL)-cholesterol concentration was significantly increased in SMS-fed groups. In addition, 20% SMS in the diet increased serum iron and testosterone concentrations in elk. These results indicate that adding SMS to the diet of elk can increase their Hgb, serum BUN, glucose, and HDL-cholesterol concentration; therefore, diets containing SMS may enhance the physiologic condition of elk during growth.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1996.06c
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• pp.692-700
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• 1996

The objective of this study is to develop a systematic purification plant using the metabolism of aerobic microorganisms. This system is subsequently aerated and continuously removes suspended solids and settling sludges caused by aerating pressure at the bottom of a lower pipe (i.e., Continuous Removal of Suspended solids and Settling sludges, CRSS). The CRSS plants are brought out by introducing fine air bubbles into the liquid phase of a lower pipe in the bio-reactor. These plant uses aeration pipe, with multiple inlets to sweep the floor of bio-reactor tank, instead of the conventional scraper mechanisms. The principal advantage of this system is that it can continuously remove very small or light particles that settles completely within a short time. Once the particles have been floated to the surface, they can be moved into the pipe and collected in the settling tank by sequently aerated pressure. The experimental results shows that about 99.0% of the biochemical oxygen demand(BOD), 99.3% of the suspended solid(SS), 92.3% of the total nitrogen(T-N), 99.0% of the turbidity(TU), 100% of the total coliform(TC)and ammonia was respectively removed during aerobic digestion for 9 days. These result indicates that the CRS S plants are very effective for reduction and deodorization of swine wastewater contaminants, and the efflux from CRS S can either be discharged in the river or used as nutrient solution of formulation for plant growth factories. The developed CRSS plant proved to be flexible and it can simply be adapted to any type of biological waste treatment problem.roblem.

• Korean Journal of Veterinary Research
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• v.39 no.1
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• pp.118-125
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• 1999

Porcine Proliferative Enteropathy(PPE) is an infectious enteric disease and a major cause of economic loss in swine industry due to weight loss, poor growth and sudden death in growing and finishing pigs at 6 to 20 weeks of age. PPE has been diagnosed by clinical signs, syndrom and lesions in the intestine in Korea. However, the diagnostic method had several problems in the detection of infected or carrier pigs. Therefore, in this study, we established the polymerase chain reaction(PCR) which was a fast, specific and sensitive method for identification of Lawsonia intracellularis (L intracellularis). We designed and synthesized primer on the 16S rDNA and p78 gene encoding L intracellularis. Specificity of the method was confirmed by comparison of the PCR results using other enteric bacteria and the study has shown that PCR method was sensitive to detect 1ng of genomic DNA as a template. Identity of the PCR products was confirmed by comparison of pattern of restriction endonuclease analysis with restriction enzyme Hae III and Pst I. Also, the PCR method was applicable to the naturally affected pigs with PPE. Based on the results from this study, the PCR method could be used as a fast and specific diagnostic tool for PPE.