Effects of two types of formula designed for commercial product on forced-swimming capacity and related biochemical parameters of Sprague-Dawley rats were evaluated. Treatment groups were fed diet supplemented with 4.56% formula A (EFA: exercise-trained and formula A-supplemented group) or B (EFB: exercise-trained and formula B-supplemented group) for 4 weeks. All groups were regularly trained twice a week to be accustomed to swimming. After 4 weeks, each group was divided into two subgroups: 90 min swimming (90-min subgroups) and swimming until exhaustion (all-out subgroups). Serum was collected, and skeletal muscles were dissected out after swimming. Forced-swimming time until exhaustion was significantly longer (p<0.05) in EFA and EFB groups than that of exercised control group. Among 90-min subgroups, serum ammonia level of EFB group was significantly lower than that of exercised control group (p<0.05). Among all-out subgroups, serum lactate levels were not significantly different among exercised control and treatment groups, whereas serum-free fatty acid levels of EFA and EFB groups were significantly lower than that of exercised control group (p<0.05). In conclusion, EFA and EFB groups enhanced forced swimming capacity of rats by increasing fat utilization and delaying accumulation of serum fatigue factor.
Swimming performance of fish is an important factor in the survival of fish. Also, swimming performance of fish is used in the form of habitat, or as a condition to consider when creating a fish ladder. However in Korea, researches in swimming performance of Korean freshwater fish were scarce and inadequate in some part, thus fish ladders were installed without considering their swimming performance. Therefore, in this study, we measured swimming performance of 4 Korean freshwater fish species to consider importance of swimming performance test. The fish used in this study were Carassius auratus, Zacco koreanus, Gnathopogon strigatus, Acheilognathus lanceolata intermedia species which was collected during October to November, 2018 at Geum River, and measurement for swimming speed of each fish was done by using $Loligo^{(R)}$ System, swim tunnel respirometer in January to February of 2019. The average value of the burst critical swimming speed ($U_{crit}$) for each species was $0.8{\pm}0.04m\;s^{-1}$ for C. auratus, $0.77{\pm}0.04m\;s^{-1}$ for Z. koreanus, $0.95{\pm}0.04m\;s^{-1}$ for G. strigatus, $0.73{\pm}0.03m\;s^{-1}$ for A. lanceolata intermedia and the average value of prolonged critical swimming speed was $0.54m\;s^{-1}$ for C. auratus, $0.67m\;s^{-1}$ for Z. koreanus, $0.7m\;s^{-1}$ for G. strigatus, $0.54m\;s^{-1}$ for A. lanceolata intermedia. Since the fish used in this experiment were collected from a small part of the water system in Korea and there were only 4 species, they were not enough to represent the species that inhabit the entire Korean water system. It will be necessary to continue evaluating the swimming performance of other freshwater species in Korea.
Purpose: This study was conducted to investigate the short-term effects of Ginseng Radix Alba extract solution on muscle fatigue and concentration of blood components especially in glucose and lactate dehydrogenase of the rats. Methods: The 4~6-week-old ICR rats are used in the study, and we administered the extract solution of Ginseng Radix Alba concentration of 1, 10, 100mg/0.3ml to each rats and water to another rats(control group) once a day for each 30 and 60 days. After 30 and 60 days. we measured the persistent time of swimming exercise test and the results of grip strength test. And we also examined the plasma concentration of glucose, and lactate dehydrogenase. Results: In swimming exercise test, the swimming time of the 1, 10, 100mg/0.3ml groups for 30 days and the 100mg/0.3ml group for 60 days significantly increased than control group. and we found that as the concentration increased, the swimming time was also increased for 30 and 60 days experiment. And in grip strength test, the grip strength of the 1, 10, 100mg/0.3ml groups for 30 days and the 10mg/0.3ml group for 60 days significantly increased than control group. and we found that as the concentration increased, the grip strength was also increased for 30 days experiment. Plasma concentration of lactate dehydrogenase was significantly increased in the 10mg/0.3ml group for 60 days than control group. And there was no significant differences between the control and the treatment group in the plasma concentration of glucose. Conclusion: This study show that Ginseng Radix Alba can increase the muscle strength and antifatigue effect.
Journal of the Korean Society of Fisheries and Ocean Technology
/
v.44
no.1
/
pp.20-30
/
2008
In order to evaluate the biodegradability of bait used in the pot for swimming crab, water tank experiments were conducted. Mackerel is the most commonly used natural form of bait to catch the swimming crabs, and therefore was used in this experiment for the biodegradability according to the manufacturing process of the bait. From the biodegradability test on chemical oxygen demand(COD), total nitrogen(T - N), total phosphorus(T - P), ammoniac nitrogen, nitrite nitrogen and nitrate nitrogen per unit weight of the bait based on the production rate and the accumulated amount of pollutants, it was concluded that the smaller the size of the mackerel pieces, the higher the production rate and accumulated amount of organic matter and nutrients which was unfavorable to water pollution. The amount of pollutants released from the intestine of the tuna was similar with that from the whole mackerel. For the operation period of 111 days, the accumulated concentrations of tested pollutants from the tuna which were 67.3 mgCOD/g d, 86.4 mgT N/g d, 3.1 mgT - P/g d, were almost half comparing with those from the mackerel which were 65.7 - 94.4 mgCOD/g d, 83.8 - 109.4 mgT - N/g d, 3.1 - 5.2 mgT - P/g d. The amount of pollutants released from the intestine of the tuna was slightly less than that from the mackerel that was cut into 8 pieces. but more than that from the mackerel which was not cut into pieces. Therefore, it can be concluded that the key factor in determining water pollution potential is not the kind of bait, but the processing or preparation method used.
The purpose of this study is to investigate the change in the positive psychology movement of capital represented by the performance of physical disabilities to participate in swimming classes for 10 weeks. The study was conducted with 30 to 40-men group participation handicapped total of 21 patients (10 patients) and non-participation group (11 patients), the duration of the experiment was performed three times a week for 10 weeks, 50 minutes. Data processing is a dictionary, post-test data was calculated the mean and standard deviation, experimental design group two won repeated measures analysis of variance for (swimming participating groups, miserable Lady) and time (before and after) using the SPSS 21.0 statistical program It was performed (2-way [2] RM ANOVA), all the statistical significance level was set at .05. Study, classroom participation of the handicapped swimmer can see that has had a positive effect positive psychological capital.
Park, Na-Ri;Lee, Yun-Jin;Lee, Eun-Byeol;Yang, Doo-Hwa;Woo, Chang-Hoon;An, Hee-Duk
Journal of Korean Medicine Rehabilitation
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v.30
no.2
/
pp.37-46
/
2020
Objectives In this study, we analyzed the effect of Dioscoreae Rhizoma on exercise practice ability and blood fatigue variable factor in the forced swimming test. Methods Male mice were divided into four group. Mice were administrated with saline, Discorae Rhizoma extracted by water (DRW), Discorae Rhizoma extracted by 30% ethanol (DRE), red ginseng (RG), orally once per day, for 1 week. Exercise performance was evaluated by exhaustive swimming time and weights. Glycogen contents of liver and soleus muscle were measured at the end of the experiment. In addition, the biochemical parameters such as asparate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen, creatine, lactate dehydrogenase (LDH), ammonia, triglycerides (TG), total cholesterol (TC) and free fatty acid (FFA) were analyzed. Results Both DRW and DRE showed an improvement of swimming endurance compared with control group. Levels of ALT, ammonia, TG, TC and FFA were significantly decreased in both DRW and DRE compared with control group. Levels of AST, Creatine were significantly reduced in DRE group compared with control group. Moreover LDH showed only a tendency to increase in the DRW and DRE groups. Conclusions In summary, the present study showed that Dioscoreae Rhizoma improved in physical fatigue and exercise practice ability and DRE improved the superior than those of DRW.
Journal of the Korean Society of Fisheries and Ocean Technology
/
v.40
no.1
/
pp.37-46
/
2004
In order to increase fishing efficiencies of the fyke net used in the coast of Jeju Island, water tank experiment was caried out entering and escaping behavior using nets that were reduced to 1/20 of the size of the full scale fyke net and were improved to have antrance structure, and mackerel Scomber japonicus as experimental fish. The results of measurement are as follows : 1. Fish school behavior in the main net was showed two different patterns : swimming in a circle in the right space of the main net and swimming back and forth in ellipse in the right and left space. 2. The swimming speed of mackerel school was 23.9. 12.6 and 32.0cm/sec in the center space, right space of main net and in the mouth 3. The entering rate of fish school was 40% in net with 35cm length of the upper and funnel net in the mouth of fyke model net and 49% in conventional type fyke model net. 4. The escaping rate of fish school was 10% in net with 35cm length of the upper and funnel net in the mouth of fyke model net and 69% in conventional type fyke model net. 5. The remain rate of fish school was 90% in net with 35cm length of the upper and funnel net in the mouth of fyke model net and 31% in conventional type fyke model net.
Objective: The purpose of this study was to evaluate effects of goat milk and fermented goat milk on reproductive function and stamina of male rodent. Methods: Experiment I: Male ICR mouse was divided into four groups. Group 1 none-treated control; Group 2 received saline; Group 3 received cow milk 10 ml/kg per day for 15 days; Group 4 received goat milk 10 ml/kg per day for 15 days. The cauda epididymal sperm motility and testicular sperm production were investigated. Experiment II: Male SD rat was divided into three groups. Group 1 received saline; Group 2 received goat milk 10 ml/kg per day for 28 days; Group 3 received fermented goat milk 10 ml/kg per day for 28 days. The cauda epididymal sperm motility and testicular sperm production were also investigated. The concentration of testosterone in serum at 1 and 3 weeks after treatment was determined using Immulite 2000 kit. Testes, epididymis, prostate, and seminal vesicle were weighed. Experiment III: Male ICR mouse was divided into four groups. Group 1 none-treated control; Group 2 received saline; Group 3 received goat milk 10 ml/kg per day for 4 weeks; Group 4 received fermented goat milk 10 ml/kg per day for 4 weeks. After treatment, the mouse was forced to swim to test for stamina. Results: In Experiment I, the cauda epididymal sperm motility after in vitro culture for 1 or 3 h was significantly (p<0.05) higher in cow milk and goat milk than in the control and saline. There was no significant difference in the cauda epidymal sperm motility between cow and goat milk. The testicular spermatid number was significantly (p<0.01) higher in goat milk (222.8${\times}10^6$) than in the control (108.6), saline (98.2), and cow milk (118.2). In Experiment II, the cauda epididymal sperm motility after in vitro culture for 1 h was significantly (p<0.05) higher in fermented goat milk than in saline and goat milk. There was no significant difference in the cauda epidymal sperm motility between saline and goat milk but goat milk showed slightly higher sperm motility than saline. After in vitro culture for 3 h, the cauda epididymal sperm motility was significantly (p<0.01) higher in fermented goat milk and goat milk than in saline. The testicular spermatid number was significantly (p<0.05) higher in goat milk than in saline, and significantly (p<0.01) higher in fermented goat milk than in saline. And the serum testosterone levels of rats administered with goat milk or fermented goat milk were increased but were no significant difference among three groups. Also the prostate weight was significantly (p<0.05) increased in the goat and fermented goat milk. In Experiment III, the swimming time in the goat milk and fermented goat milk groups was significantly (p<0.01) longer than in the control and saline. There was no significant difference in the swimming time between goat and fermented goat milk but the fermented goat milk showed slightly longer swimming time than the goat milk. Conclusion: The cauda epididymal sperm motility, the testicular spermatid number and stamina were improved when the mice and rats were drunk with goat milk or fermented goat milk.
Objectives : The purpose of this study was to assess anti-depressive effects of Bee Venom(BV) on an Animal Model of Depression induced immobility stress. Methods : There was 2 pre-experiments MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and Western blot test and 3 main experiments ; forced swimming test, tail suspension test and Y-maze task. Male rats were used for main experiment. The subject was divided into 4 groups(1. control group injected only saline, without immobility stress 2. Negative group injected saline after 2 hours immobility stress 3. Positive group injected Amitriptyline after 2 hours immobility stress 4. BV group injected Bee Venom after 2 hours immobility stress). Each group consisted of 6 rats. Forced swimming test, tail suspension test, Y-maze task were used to evaluate anti-depressive effect of Bee Venom. Results : In MTT assay, as the density of BV increased, the existence rate of primary neuronal cell increased. In Western blot test, the density of CREB and AKT was increasing as time went by. In forced swimming test, BV group showed immobility decreased more than Normal group and Positive group. In tail suspension test, Normal group and Positive group showed immobility decreased more than BV group. In Y-maze task, BV group showed immobility decreased more than Normal group, but Positive group showed immobility decreased more than BV group. Conclusions : These results suggest that Bee Venom may have anti-depressive effect on depression.
Objective : This study was designed to assess the protective effects of Chengwhabosimtang on the animal model of depression, chronic mild stress(CMS). Method : Male Sprague-Dawley rats Were used for this experiment. The subjects Were divided into 3 groups (1. CMS-drug: Chengwhabosimtang administered during CMS treatment, 2. CMS-vehicle: water administered, 3. normal ). After 4 weeks of CMS treatment they were executed Forced swimming test(FST) and Elevated plus maze. Tyrosine hydroxylase(TH) in ventral tegmental area(VTA) and c-Fos in paraventricular nucleus(PVN) were measured. Result : 1. In FST, CMS-drug group showed significantly decreased immobility behavior. 2. CMS-drug group showed no significantly lower TH level in VTA than CMS-vehicle group. 3. CMS-drug group showed significantly less c-Fos expressed cell bodies in PVN than CMS-vehicle group. 4. In Elevated plus maze, CMS-drug group showed no significantly anxiety. Conclusion : These results suggest that Chengwhabosimtang may have protective antidepressant effects in CMS model rats. And these effects could be explained by the elevated stress-copying behaviors which are related with PVN of hypothalamus and dopaminergic neurons in VTA.
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