• Bulletin of the Korean Chemical Society
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• 제30권7호
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• pp.1445-1451
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• 2009

New benzotriazoles (5-8) or dibenzodiazepine derivatives (11-18) were synthesized starting from 3-[(2-amino- 4,5-disubstitutedphenyl)amino]-5,5-disubstitutedcyclohex-2-enones (1-4) through internal coupling of their diazonium salts or internal Mannich reaction in the presence of aromatic aldehydes. Pharmacological evaluation of benzotriazole and dibenzodiazepine derivatives for their clozapine-like properties revealed that dibenzodiazepine 11 bearing 4-bromophenyl group exhibited the same antipsychotic activity as the reference drug clozapine while the activity of benzotriazole 7 was 25% lesser than that of clozapine. Moreover, compounds 7 and 11 did not show significant CNS depressant activity as well as no or slight neurotoxicity on contrast to clozapine when tested in mice using forced swim, rotarod and horizontal screen tests.

• Journal of Magnetics
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• 제21권4호
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• pp.616-621
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• 2016

This paper presents a new multifunctional active guidewire system for medical applications that uses a magnetic microrobot. The study demonstrated that the proposed microrobot system could swim and be controlled under Low-Reynolds-number (Re) environments in blood vessel models. The prototype of the robotic guidewire, which is driven within a three-axis Helmholtz coil system, consists of a guide-wire, spiral blade, drilling tip, and permanent magnet. The spiral-type microrobot showed stable active locomotion between 3 kA/m and 9.1 kA/m under driving frequency up to 70 Hz in a silicone oil (of viscosity 1000 cst). The microrobot produced a maximum moving velocity of $8.08{\times}10^{-3}m/s$ at 70 Hz and 9.1 kA/m. In particular, the robotic guidewire produced 3D locomotion with drilling in the three-axis Helmholtz coil system. We verified active locomotion, towing of guidewire, steering, and drilling of the proposed robotic guidewire system through experimental analyses.

• 운동영양학회지
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• 제13권1호
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• pp.23-28
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• 2009

The expression of c-Fos and c-Jun represents neuronal activity and plays a crucial role in the shaping of the development of brain. During the late pregnancy, exercise is known to influence neuronal activity of offspring. In the present study, the effect of swimming during pregnancy on the expression of c-Fos and c-Jun in the CA1, CA2, CA3 regions, and the dentate gyrus of the hippocampus of rat offspring was investigated using immunohistochemistry. Pregnant rats in the swimming group were forced to swim for 10 min once a day from 15 days after pregnancy until delivery. The expression of c-Fos and c-Jun in the CA1, CA2, CA3 regions, and the dentate gyrus of the hippocampus of pups was significantly increased by maternal swimming during late pregnant period. The present results show that prenatal swimming may enhance the neuronal activity of pups and affect the neonatal brain development.

• 정신신체의학
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• 제8권1호
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• pp.46-57
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• 2000

연구목적 : 본 연구는 선택적 세로토닌 재흡수 차단제인 paroxetine과 sertraline의 마우스에 대한 아급성 처치가 강제수영시험에서의 부동자세 시간과 강제수영시험으로 유발된 면역능의 변화에 미치는 영향을 조사하고자 시행되었다. 방법 : 저자들은 실험동물로 각 군마다 5마리 이상의 BALB/c 마우스를 사용하였다. 본 실험동물에서의 적절한 모델을 구축하기 위하여 Porsolt 등의 강제수영시험을 다소 변형하여 각각의 실험 대상군에 적용하였다. 강제수영시험으로 인한 면역 매개변수의 변화를 보기 위하여, 저자들은 anti-rat RBC 항체의 생성, concanavalin 또는 lipopolysaccharide로 유발된 비장세포의 증식과 사이토카인의 유전자 발현 양상을 연구하였다. 결과 : Paroxetine과 sertraline은 모두 투여량에 따라 마우스의 부동자세 시간을 감소시켰다. 강제수영시험을 수행한 마우스는 비세포의 유사분열 반응이 의미 있게 감소되었고, anti-rat RBC 항체는 약간 증가하였다. 이러한 모든 반응은 paroxetine에 의해 의미 있게 감퇴되었고. sertraline에 의해 약간 감퇴되었다. 강제수영시험을 시행한 마우스에서 Con-A로유발된 비세포는 강제수영을 시행하지 않았던 정상대조군보다 IL-4의 강한 발현과 IL-2의 약화된 발현을 보였고, IFN-$\gamma$나 lymphotoxin의 발현은 차이가 없었다. IL-6과 IL-10은 양 군 모두에서 발현되지 않았다. 마우스에서 paroxetine과 sertraline의 전처치는 강제수영으로 인한 사이토카인 발현의 변화를 감퇴시켰다. 그렇지만 선택적 세로토닌 재흡수 차단제가 전처치된 마우스에서는 강제수영군에서 발현되지 않았던 IL-6과 IL-10의 발현이 약간의 변화를 보였다. 결론 : 강제수영시험을 시행한 마우스에서 paroxetine과 sertraline의 전처치는 강제수영시험으로 유발된 행동 및 면역능의 변화를 감퇴시켰다. 이러한 선택적 세로토닌 재흡수 차단제는 사이토카인 유전자 발현 특히 IL-6과 IL-10의 유도를 통하여 면역 체계에 모종의 조절 효과를 발휘하는 것으로 추정되었다.

• 생태와환경
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• 제52권2호
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• pp.118-125
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• 2019

어류의 유영능력은 어류의 생존을 결정짓는 중요한 요인이다. 또한, 어류의 유영능력 측정값은 어도 설치 시 서식하는 종에 대한 기초자료, 생태 하천 복원 시 어류의 서식 및 산란장 조성, 생태유량 산정 시 자료로 활용할 수 있다. 그러나 국내의 경우 담수어류의 유영능력에 대한 연구가 거의 이루어지지 않거나, 부족한 면이 있기 때문에 어류의 유영능력이 고려되지 않은 상태에서 어도들이 설치되어 왔다. 따라서 본 연구에서는 금강 수역에서 서식하는 붕어(Carassius auratus), 참갈겨니(Zacco koreanus), 줄몰개(Gnathopogon strigatus), 납자루(Acheilognathus lanceolata intermedia)를 대상으로 유영능력측정기 (Swim tunnel respirometer, $Loligo^{(R)}$ System)를 사용하여 돌진속도, 전진속도를 포함한 유영속도를 측정하였다. 종별 평균 돌진속도의 $U_{crit}$ (임계유영속도) 값은 붕어(C. auratus) $0.8{\pm}0.04m\;s^{-1}$; 참갈겨니(Z. koreanus) $0.77{\pm}0.04m\;s^{-1}$; 줄몰개(G. strigatus) $0.95{\pm}0.04m\;s^{-1}$; 납자루(A. lanceolata intermedia) $0.73{\pm}0.03m\;s^{-1}$으로 측정되었고, 각 종별 평균 전진속도의 $U_{crit}$ 값은 C. auratus $0.54m\;s^{-1}$, Z. koreanus $0.67m\;s^{-1}$, G. strigatus $0.7m\;s^{-1}$, A. lanceolata intermedia $0.54m\;s^{-1}$로 측정되었다. 본 실험에서 사용된 어류는 금강 수계에서 채집된 담수어류 중 4종만을 선정하여 실험이 진행되었기 때문에 한국의 전체 수계에 서식하는 해당 종을 대표하기에는 부족한 면이 있지만, 해당 수계에 서식하는 종의 특성 자료로 활용될 수 있을 것으로 판단된다. 따라서 향후 국내에 서식하는 보다 다양한 담수어류에 대한 유영능력 평가가 필요하며, 이들 자료를 활용한 어도를 통한 하천의 연결성 확보가 요구된다.

• 한방안이비인후피부과학회지
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• 제26권3호
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• pp.36-53
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• 2013

Objectives : The purpose of this study is to investigate the effects of DOGO phreatic water containing sulphur on Atopic Dermatitis in NC/Nga mouse. Methods : We made DOGO phreatic water removed sulphur using Twin Alternating Sulfate Eater. After making atopic dermatitis caused by sensitizing NC/Nga mouse to DNCB(dinitrochlorobenzene), we made mouse swim in tanks each filled with distilled water, tap water, DOGO phreatic water(contain sulphur), DOGO phreatic water(remove sulphur) for 30minutes everyday. 3weeks later, we analyzed skin clinical score, total IgE levels(by ELISA), WBC differential counting(Neutrophils, Monocytes), absolute cell number of $Neutrophil^+Gr-1^+$, CCR3 mRNA expressions(by Real-time PCR), IL-4, IFN-${\gamma}$ production levels(by ELISA), histologic test(by H&E staining, toluidine blue staining). Results : The results of making NC/Nga mouse induced atopic dermatitis swim in tanks filled with DOGO phreatic water(contain sulphur) are as follows. 1. Skin clinical scores were decreased significantly in comparison to control group. 2. Total IgG levels were decreased significantly in comparison to control group. 3. WBC differential counting(Neutrophils, Monocytes) were decreased significantly in c.mparison to control group. 4. Absolute cell number of $Neutrophil^+Gr-1^+$ were decreased significantly in comparison to control group. 5. CCR3 mRNA expressions were decreased significantly in comparison to control group. 6. IL-4, IFN-${\gamma}$ production levels were decreased significantly in comparison to control group. 7. The epithelial tissue thickness, leucocytes infiltration, erythema, edema, excoriation, scaling, mast cells infiltrations in dorsal skin were decreased in comparison to control group. Conclusions : These results indicate that DOGO phreatic water(contain sulphur) can be used for helping treat atopic dermatitis.

• 한국산학기술학회논문지
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• 제15권1호
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• pp.367-376
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• 2014

이 연구의 목적은 건강한 남자 14명을 대상자로 단기간의 지구성 훈련과 지구성 운동에 웨이트 운동을 병행한 복합훈련이 골격근 섬유의 형태 및 골격근 내 산화적 효소의 활성도에 미치는 영향을 구명하고자 하였다. 지구성 트레이닝 집단은 최대심박수의 70~80% 에서 40분간 자유형으로 수영을 6주간 실시하였으며 복합 트레이닝 집단은 동일한 수영을 실시한 후 웨이트 트레이닝을 추가로 실시하였다. 근섬유 횡단면적에 있어 복합훈련 집단과 지구성 훈련 집단 간에 차이가 나타나지 않았으며 훈련기간에 따라 복합훈련 집단에서 지구성 훈련 집단보다 증가한 것으로 나타났다. 모세혈관 수에 있어서도 집단 간 차이는 나타나지 않았으며 훈련기간에 따라 복합훈련 집단에서 지구성 훈련 집단보다 증가한 것으로 나타났다. 유산소성 대사 효소 중 CS, ${\beta}$-HAD 그리고 CPT의 활성도는 훈련형태에 따른 차이는 통계적으로 나타나지 않았으나 복합훈련 집단에서 지구성 훈련 집단에서 보다 더 큰 증가의 폭을 나타냈다. 이와 같은 결과를 종합해 볼 때 유산소성 훈련만을 실시할 때 보다 유 무산소 운동이 복합적으로 구성되어 있는 훈련의 수행이 근섬유의 에너지 대사 관련 효소들의 활성도 증가와 근 세포 모세혈관의 발달에 보다 효과적이라는 결론을 도출할 수 있었다.

• 대한수의학회지
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• 제32권3호
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• pp.435-450
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• 1992

To evaluate the fertilizing capacity of domestic animal spermatozoa by hamster test, semen were collected from 15 boars(Duroc, Landrace, and Yorkshire) and 2 mixed dogs which had been proved to be fertile in the past then, the semen were preserved in BWW medium at $4^{\circ}C$ or $18^{\circ}C$ for about 20 hours and coincubated with zona-free hamster ova for 5 hours. The ova were stained by lacmoid and examined under phase contrast microscope to investigate the rates of sperm binding to the ova, penetration and formation of a male pronucleus, and the numbers of both bound and penetrated sperm per ovum. Both the semen preserved at $18^{\circ}C$ for about 20 hours and that treated by swim up procedure showed considerably higher rates of sperm binding and penetration as well as higher number of penetrated sperm than that preserved at $4^{\circ}C$ for about 20 hours, respectively(p<0.01). Motility of boar sperm at insemination was from 40 to 90% and no difference in hamster test was obtained according to different degree of sperm motility. Abnormality in morphology of boar sperm at insemination was from 6 to 45% and no difference in hamster test was obtained according to different degree of sperm abnormality. The sperm concentrations of $7{\times}10^7$ and $7{\times}10^6$ showed considerably higher rates of sperm binding and penetration as well as higher number of bound sperm than that of $7{\times}10^4$ (p<0.01) along with the same higher results than that of $7{\times}10^5$(0<0.05), respectively. Boar sperm showed considerably higher rates of sperm binding and penetration as well as higher numbers of both bound and penetrated sperm than dog sperm, when both semen were treated by BWW＋heparin medium and swim up procedure, respectively. These results indicated that fertile boar sperm showed considerably lower rates in the results of hamster test, when preserved at $4^{\circ}C$ for about 20 hours and in lower concentration of sperm than when preserved at $18^{\circ}C$ for about 20 hours and in higher concentration of sperm, respectively, and at the same time considerably higher results than fertile dog sperm, consequently to prove that hamster test would be of great value in assaying the fertilizing capacity of boar sperm.

• Clinical and Experimental Reproductive Medicine
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• 제21권1호
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• pp.1-11
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• 1994

The occurrence and time course of capacitation, acrosomal loss, and hyperactivated motility require quantitative definition in order to characterize fertile human sperm. Recently the method has been developed to estimate the quality of spermatozoa by using kinematic parameters such as curvilinear velocity(VCL), average path velocity(VAP), linearity(LIN), straightness(STR), amplitude of lateral head displacement(ALH), and beat cross frequence(BCF) from Computer Assisted Sperm Analysis (CASA). In this study, using the Hamilton Thorn motility analyzer HTM 2030(Hamilton Thorn Research, Beverly, MA), we attempted to identify the spermatozoa with hyperactivated motility (HA) objectively and to monitor hyperactivation of human spermatozoa during incubation in capacitating media and after treatment of calcium ionophore as compared with acrosome status. And we examined whether HA are related to the result of SPA. Semen samples obtained from 16 healthy men were prepared by swim up technique and preincubated in a capacitating media(modified BWW medium) for 5 hours and treated with calcium ionophore solution. The acrosome reaction was detected with PSA-FITC labelling of the acrosome and in vitro sperm ferilizing capacity was assessed by the zona free hamster ovum penetration assay (SPA). The incidence of hyperactivated sperm was 2.6% in fresh semen, 14.3% of the swim up population, 13.7% after 5h of incubation. Significant increase of percentage of hyperactivated sperm was observed after the incubation (p<0.05) but after treatment, no significant changes of percentage of hyperactivated sperm(l1.8%) in contrast to significant rise in the percentage of acrosome reacted cells. Correlation analysis failed to show any significant relationship between the percentage of sperm with HA and SPA score. In conclusion, although no direct correlations were found between the results of SPA and HA, hyperactivation of sperm is associated with capacitation and monitoring hyperactivated sperm will be expected as a method of evaluating the functional quality of sperm such as SPA.

• Clinical and Experimental Reproductive Medicine
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• 제20권2호
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• pp.107-115
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• 1993

Procedures to separate motile. normal & motile and acrosome-reacted sperm with high efficiency have clinical application in Assisted Reproductive Technology in terms of increasing the probability of fertilization by a normal sperm and subsequent normal embryonic development. This study evaluated the effects of 10 sperm preparation techniques [Swim-up from a washed pellet (SU). Swim-up from semen (SO). Continuous Percoll Gradients I (PIC). Discontinuous Percoll Gradients I (PID). Continuous Percoll Gradients II(P II C). Discontinuous Percoll Gradients II(P II D), SpermPrep (SFC). Wang's tube (WT). Albumin Gradients (AG), Low temperature capacitation (LTC)] on motility (%), normal morphology (%), motile sperm recovery rate(%). morphologically normal & motile sperm recovery rate (%), true acrosome reaction (%) and fertilizing ability. A P II D proved to be an effective means of separating morphologically normal & motile sperm. Our results indicated the P II D has advantages as compared with other methods in terms of recovery rate. enhancement of motility and normal morphology. And a LTC seems to be an effective means of enhancing the true acrosome reaction and fertilizing ability. These results suggest that the combined method of LTC and P II D for separation of morphologically normal & motile sperm and acrosome reacted sperm may be a useful procedure for intrauterine insemination and in vitro fertilization in the management of male factor infertility as well as for isolation of subpopulation of sperm for basic research.