• 한국진공학회:학술대회논문집
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• 한국진공학회 2013년도 제45회 하계 정기학술대회 초록집
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• pp.268.1-268.1
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• 2013

Immobilization of conducting nanoparticles on a nanogap comprising two electrodes spaced at a distance comparable to the particle size can be used as a simple and sensitive method of detecting the particles. In this work, we have examined the performance of the nanogap devices in the measurement of metallic nanoparticles, particularly gold nanoparticles (Au NPs). Detection of pM-level Au NPs in an aqueous suspension was quite straightforward irrespective of the existence of non-conducting materials. Speed of detection or the time necessary for the completion of the measurement, however, was strongly dependent upon the immobilization process. Active trapping process was found to be much more efficient and also effective in the detection of nanoparticles than its passive counterpart.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVI)
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• pp.37-37
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• 2005

Human chorionic gonadotropin (hCG) is a heterodimeric glycoprotein hormone consisting of non-covalently linked two subunits, the ${\alpha}$ and ${\beta}$ subunit. It has been used as a infertility drug for ovulation to mimic luteinizing hormone $(LH).^{1)}$ A stable cell line was established by transfection of Rc/CMV-i-dhfr-hCG, expression vector containing hCG ${\alpha}-$ and ${\beta}-genes$, into dihydrofolate reductase-deficient CHO cells and subesquent methotrexate-mediated gene amplification. Anchorage-dependent CHO cells were adapted into a serum-free and/or animal component-free suspension medium through gradual serum weaning for the hCG production. The established cell line showed typical morphological characteristics and growth profile of CHO cells, and could produce FSH with passage-to-passage consistency. The high density perfusion culture of the CHO cells was carried out in Celligen Plus bioreactor equipped with a spin-filter as a internal cell retention device. The cell density reached up to $>1x10^{7}$ cells/ml in less than 7 days and a perfusion-control strategy based on cellular consumption rates of glucose was $established.^{2)}$ Biologically active recombinant hCG was purified by a series of chromatographic steps including anion exchange chromatography and hydrophobic interaction chromatography to homogeneity. The highly purified recombinant hCG was characterized for physicochemical, immunological and biological properties.

• 한국철도학회논문집
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• 제20권3호
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• pp.311-319
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• 2017

철도차량의 1차 현가장치는 윤축과 대차를 구속하는 장치로써 각 방향의 강성에 따라 차량의 동특성에 큰 영향을 미치며, 동특성을 향상시키기 위해서는 각 방향 강성을 다르게 요구하는데 일반적인 현가장치의 형상으로는 각 방향의 강성을 다르게 설계하기란 어렵다. 따라서 본 논문에서는 코니칼 러버 스프링(Conical rubber spring)을 이용하여 각 방향의 강성을 다르게 설계할 수 있도록 최적화 기법을 적용하여 목표값과 해석값의 RMS(Root Mean Square) 값을 이용하여 최적화를 수행하고 최적형상을 토대로 모델의 취약부의 형상을 보완하여 최종 모델을 제안한다. 실제 모델을 개발하여 정하중 시험을 통해 목표 강성값과 약 7.7%의 편차평균을 나타내 최적화 모델의 신뢰성을 입증하였다. 또한 최종 강성값을 다물체 동역학 모델에 적용하여 안정성과 곡선 주행성능 해석을 수행하였으며 적용모델의 임계속도는 대상 모델의 주행 최고속도인 110km/h 보다 높은 190km/h이며 차륜의 마모지수는 기존대비 34% 감소하여 조향 성능이 향상되었음을 확인하였다.

• Journal of Pharmaceutical Investigation
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• 제24권1호
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• pp.41-48
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• 1994

A new local drug delivery device to treat otitis media (OM) has been developed. This device consists of a biodegradable poly(L-lactic acid) (PLLA) film containing antibiotic (ampicillin, AMP), which can be placed into the middle ear cavity and release the therapeutic concentration of AMP for prolonged period. Biodegradable films containing AMP (10 w/w%) were prepared by solution casting method using a suspension of the drug in a $PLLA/CH_{2}Cl_{2}$ solution (molecular weight of PLLA, 100,000 (100 K) and 300,000 (300 K), respectively). PLLA-AMP films were characterized by FTIR, DSC, and SEM. In vitro release of AMP from AMP-PLLA films were examined. The release pattern of AMP from AMP-PLLA films remained consistent from 1 day to 14 days, and the release rates of AMP from AMP-100K-PLLA film and AMP-300K-PLLA film were $0.7384\;{\mu}g/ml/day$, $0.4107\;{\mu}g/ml/day$, respectively.

• Journal of Advanced Marine Engineering and Technology
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• 제38권2호
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• pp.217-224
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• 2014

We have developed the novel device that can extract energy from ocean waves utilizing the heaving motion of a floating mass. The major components of the energy converter are: a floater, a counterweight, a cable, a driving pulley, two idler pulleys, a ratchet, and a generator. The device generates power through the tension force in the cable and the weight difference between the floater and the counterweight. When the system is at static free condition, the tension in the cable is equal to the weight of the counterweight which is minimum. Therefore it is desirable to keep the counterweight lighter than the floater. However, experiments show that during the rise of the water level, the torque generated by weight of the counterweight is insufficient to rotate the driving pulley which causes the cable on the floater side to slack. The proposed application of the tension pulley rectifies these problems by preventing the cable from becoming slack when the water level rises. In this paper, the dynamics model is modified to incorporate the dynamics of the tension pulley. This has been achieved by first writing the dynamical equations for the tension pulley and the energy converter separately and combining them later. This paper investigates numerically the effect of the tension pulley on various physical quantities such as the cable tension, the floater displacement, and the floater velocity. Results obtained indicate that this application is successful in suppressing large fluctuations of the cable tension.

• Design & Manufacturing
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• 제13권1호
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• pp.55-60
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• 2019

Smart-phone in the recently released high-end applied to the camera module is equipped with the most features auto focusing camera module. Also, auto focusing camera module is divided into voice coil motor, encoder, and piezo according to type of motion mechanism. Auto focusing camera module is composed of voice coil motor (VCM) as an actuator and leaf spring as a guide and suspension. VCM actuator is made of magnet, yoke as a metal, and coil as a copper wire. Recently, the assembly as yoke and magnet is made by human resources. These process has a long process time and it is difficult to secure quality. Also, These process is not economical in cost, and productivity is reduced. Therefore, an automatic assembly as yoke and magnet is needed in the present process. In this paper, we have developed an automatic assembly device that can automatically assemble yoke and magnet, and performed verifying performance. Therefore, by using the developed automatic assembly device, it is possible to increase the productivity and reduce the production cost.

• 한국식품과학회지
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• 제25권2호
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• pp.171-173
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• 1993

냉장고내 공기의 청정장치로 자외선 공기순환식 살균장치를 개발할 목적으로 6 watt 자외선 램프와 순환 fan을 사용하여 공기 청정장치를 설계, 제작하였다. 자외선량은 Bacillus subtilis 포자의 D value의 5배에 이르게 하였으며 순환공기의 유속은 0.785 m/s이었으며 공기의 살균부 체류시간은 0.382 s이었다. 동 장치를 실내공기, 부패 건초 분말과 Bacillus subtilis 포자 현탁액으로 오염시킨 공기를 실온에서 80% 청정시키는 살균효과를 거두어 냉장고용 공기청정기를 설계하는데 필요한 자료를 얻었다.

• 전기학회논문지
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• 제62권7호
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• pp.1045-1052
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• 2013

In this study we deal with design procedures for the flywheel energy storage system that has the capacity to store the regenerative energy produced from the railway vehicles. The flywheel energy storage system (FESS) stores the regenerative electrical energy into the high speed rotational flywheel, by conversion the electrical energy into the mechanical rotational energy. Thus the FESS is composed of the energy conversion components, such as the motor and generator, mechanical support components, such as the rotational rotor, the magnetic bearings to support the rotor, and the digital controller to control the air gap between the rotor and the magnetic bearings. In this paper the design procedures for the rotor operating at the rigid mode and the magnetic bearings to support the rotational rotor without contact are presented.

• Journal of Electrical Engineering and Technology
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• 제8권6호
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• pp.1451-1456
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• 2013

This paper presents the improved zero power levitation control algorithm for a quadruple hybrid EMS (Electromagnetic Suspension) system. Quadruple hybrid EMS system is a united form of four hybrid EMS systems one on each corner coupled with a metal plate. Technical issue in controlling a quadruple hybrid EMS system is the permanent magnet's equilibrium point deviation caused by design tolerance which eventually leads to a limited zero power levitation control that only satisfies the zero power levitation in one or two hybrid EMS system among the four hybrid EMS system. In order to satisfy a complete zero power levitation control of the quadruple hybrid EMS system, the proposed method presented in this paper adds a compensating algorithm which adjusts the gap reference of each individual axe. Later, this paper proves the stability and effectiveness of the proposed control algorithm via experiment and disturbance test.

• 한국정밀공학회지
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• 제20권5호
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• pp.204-209
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• 2003

An elastomeric bushing is a device used in automotive suspension systems to reduce the load transmitted from the wheel to the frame of the vehicle. A bushing is an elastomeric hollow cylinder which is bonded to a solid steel shaft at its inner surface and a steel sleeve at its outer surface. The relation between the load applied to the shaft or sleeve and the relative deformation of elastomeric bushing is nonlinear and exhibits features of viscoelasticity. A load-displacement relation for elastomeric bushing is important for dynamic numerical simulations. A boundary value problem for the bushing response leads to the load-displacement relation which requires complex calculations. Therefore, by modifying the constitutive equation fur a nonlinear viscoelastic incompressible material developed by Lianis, the data fur the elastomeric bushing material was obtained and this data was used to derive the new load-displacement relation for radial response of the bushing. After the load relaxation function for the bushing is obtained from the step displacement control test, Pipkin-Rogers model was developed, Solutions were allowed fur comparison between the results of Modified Lianis model and those of the proposed model. It is shown that the proposed Pipkin-Rogers model is in very good agreement with Modified Lianis model.