• Title/Summary/Keyword: Suspended growth organism

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Effect of Copper toxicant on Suspended and Attached Growth Nitrifying Bacteria (부유 및 부착성장 질화균에 미치는 구리 독성의 영향)

  • Kim, Keum-Yong;Paek, Joo-Heon;Lee, Sang-Ill
    • Journal of Korean Society of Environmental Engineers
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    • v.31 no.10
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    • pp.855-864
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    • 2009
  • The effect of toxicant on the inhibition of nitrification was investigated, using concentrated nitrifying bacteria of both attached and suspended growth. This nitrifying organism was originally obtained from the activated sludge of sewage treatment plant and cultivated for more than three months. The object of this experiment is to determine the effect of the specific surface area and the growth condition of nitrifying bacteria on toxicity of heavy metal. The results of this study were as follows. The specific surface area of both attached and suspended growth of nitrifying organism was proven to be a major factors in determining the inhibition of nitrification of heavy metal such as $Cu^{++}$ion. When the condition of attachment and detachment was compared in an experiment using attached growth nitrifier, the effect on toxicant was 1.12 times less in attached condition than in detached condition for Nitrosomonas, and 1.09 times less for Nitrobacter. In case of suspended growth nitrifier, the effect on toxicant was 1.46 times less in non-ground condition than in ground condition for Nitrosomonas, and 1.35 times less for Nitrobacter. Also, similar results were obtained in a set of experiments, without adding nitrite to the substrate. In an experiment that compared attached condition using attached growth nitrifier with detached condition using attached growth nitrifier, the effect on toxicant was 1.83 times less in attached condition than in detached one for Nitrosomonas, and 1.78 times less for Nitrobacter. In case of suspended growth nitrifier, the effect on toxicant was 1.27 times less in non-ground condition than in ground condition for Nitrosomonas, and 1.32 times less for Nitrobacter.

GIS-Based Suitable Site Selection for Aquaculture Using Scope for Growth of Styela Clava (GIS 기반의 미더덕 SFG(Scope for Growth)를 이용한 양식장의 적지선정)

  • Park, Jung-Hyun;Suh, Yong-Cheol
    • Journal of the Korean Association of Geographic Information Studies
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    • v.16 no.3
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    • pp.81-90
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    • 2013
  • The purpose of this paper is to do GIS-based suitable site selection using Scope for Growth index of Styela clava in Jindong Bay. The aquaculture of Styela calva is only conducted in Korea, especially Jindong Bay. Suspended culture of Styela clava was initiated in 2001 and the annual production reached 15,084M/T, but declined to 1,412M/T in 2005. The annual production was increased slightly to 2,484M/T in 2012 but the production is lower than the beginning yield. Scope for Growth(SFG) can indicate interrelationships between environment and organism growth index in aquaculture. GIS-based suitable site selection can be available by the concept of SFG, and fishery management system can be constructed for the sustainable production. As a result of the assessment of habitat suitability, Jindong Bay's SFG value ranges 0.054~0.57J/day and Styela clava farm's SFG values range 0.054~0.57J/day, either. The correlation between Styela clava farm's SFG and the actual production has a good result, as r=0.786, p<0.05. The construction of fishery management system using habitat suitability index ensures the reasonable site selection and the sustainable production in aquaculture farm. It introduces an objective method for the interrelationships between the environmental variation and the organism growth. Consequently, it can promote the decision making practices for the sustainable fishery management.

A Study on Removal of Organism and Nitrogen, Phosphorus in Wastewater Treatment Process Using Nitrifier Activated Reactor (질산화균 활성화조를 이용한 하수처리 공정에서의 유기물 및 질소, 인 제거에 관한 연구)

  • Dong, Young-tak;Seo, Dong-whan;Bae, Yu-jin;Park, Ju-seok
    • Journal of Korean Society of Water and Wastewater
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    • v.21 no.6
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    • pp.727-735
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    • 2007
  • The use of water by cities is increasing owing to industrialization, the concentration of population, and the enhancement of the standard of living. Accordingly, the amount of waste water is also increasing, and the degree of pollution of the water system is rising. In order to solve this problem, it is necessary to remove organisms and suspended particles as well as the products of eutrophication such as nitrates and phosphates. This study developed a high-end treatment engineering solution with maximum efficiency and lower costs by researching and developing a advanced treatment engineering solution with the use of Biosorption. As a result, the study conducted a test with a $50m^3/day$ Pilot Scale Plant by developing treatment engineering so that only the secondary treatment satisfies the standard of water quality and which provided optimal treatment efficiency along with convenient maintenance and management. The removal of organisms, which has to be pursued first for realizing nitrification during the test period, was made in such a way that there would be no oxidation by microorganisms in the reactor while preparing oxygen as an inhibitor for the growth of microorganism in the course of moving toward the primary settling pond. The study introduced microorganisms in the endogeneous respiration stage to perform adhesion, absorption, and filtering by bringing them into contact with the inflowing water with the use of a sludge returning from the secondary settling pond. Also a test was conducted to determine how effective the microorganisms are as an inner source of carbon. The HRT(Hydraulic Retention Time) in the nitrification tank (aerobic tank) could be reduced to two hours or below, and the stable treatment efficiency of the process using the organisms absorbed in the NAR reactor as a source of carbon could be proven. Also, given that the anaerobic condition of the pre-treatment tank becomes basic in the area of phosphate discharge, it was found that there was excellent efficiency for the removal of phosphate when the pre-treatment tank induced the discharge of phosphate and the polishing reactor induced the uptake of phosphate. The removal efficiency was shown to be about 94.4% for $BOD_5$. 90.7% for $COD_{Cr}$ 84.3% for $COD_{Mn}$, 96.0% for SS, 77.3% for TN, and 96.0% for TP.

Application of 'Sponge Model' with Disinfectants for the Inhibition of Listeria monocytogenes (Listeria monocytogenes의 증식억제를 위한 살균제 'Sponge model'의 응용)

  • LEE Myung-Suk
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.29 no.5
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    • pp.595-602
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    • 1996
  • The antimicrobial effects of two disinfectants commonly used in food industry on Listeria monocytogenes ATCC 15313 were studied. The two disinfectants tested were commercial benzalkonium chloride (BAC) and sodium hypochlorite (NaOCl). Their effects were studied on cells suspended in disinfectants (in vitro) and in the sponge model with the disinfectants (in vivo). When cells were exposed to $0\~0.1\%$ BAC and $0\~150\;ppm$ NaOCL for 20 minutes, BAC and NaOCl concentration more than $0.25\%$ and 100 ppm showed the antimicrobial effects respectively. This organism decreased rapidly during the first $0.5\~1$ minute followed by a slower decrease during the rest of the exposure time. Fifteen ml of cell solution $(about\;10^7\;CFU/ml\;in\;the\;TSB)$ was mixed with 15 ml of disinfectants in the sponge $(6.0{\times}4.0{\times}4.0cm)$, BAC and NaOCl concentration more than $0.1\%$ and 300 ppm showed the antimicrobial effects, and at $0.25\%$ and 800 ppm diminished in cell numbers 3-log cycles during the first 20 minutes. In the case of sponge model, 15 ml of cell solution and 15 ml of disinfectants $(0.25\%\;of\;BAC,\;800\;ppm\;of\;NaOCl)$ were suspended in the sponge during 20 minutes, washing with 200 ml of sterilized distilled water, and this sponge was transfered in the 100 ml TBS, and then incubated at various temperature. The cells were increased about 1-log cycle during 24 hrs at $5\~15^{\circ}C$. And the others temperature, the cells growth was in proporation to storage tepmerature and the cells were about $10^9\;CFU/ml$ after $1\~3$ days incubations.

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The Geochemical Characteristics and Environmental Factors on the Marine Shellfish Farm in Namhae-po Tidal Flat of Taean (태안 남해포 갯벌 패류양식해역의 환경특성)

  • Choi, Yoon Seok;Park, Kwang Jae;Yoon, Sang Pil;Chung, Sang Ok;An, Kyoung Ho;Song, Jae Hee
    • The Korean Journal of Malacology
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    • v.29 no.1
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    • pp.51-63
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    • 2013
  • To assess the effect of environmental factors on the sustainability of cultured production shellfish, we investigated the habitat characteristics of tidal flat (Namhae-po in Taean). We measured the physiochemical parameters (temperature, salanity, pH, dissolved oxygen and nutrients) and the geochemical characteristics (chemical oxygen demand, ignition loss, C/N ratio and C/S ratio). Surface sediments were collected from several site of tidal flat to examine the geochemical characteristics of both the benthic environment and heavy metal pollution. The grain size for research area of tidal flat were similar at the ratio of silt and clay in comparison with the other site of it. The C/N ratio was more than 5.0, reflecting the range arising from the mix of marine organism and organic matter. The C/S ratio (about 2.8) showed that survey area had anoxic or sub-anoxic bottom conditions. The enrichment factor (Ef) and index of accumulation rate (Igeo) of the metals showed that those research areas can be classified as heavily polluted, heavily to moderately polluted, or more or less unpolluted, respectively. Adult surf clam (Mactra veneriformis) density was highest at St. 2 (middle part of the Namhae-po), on the other hand, surf clam spat density was highest at St. 3 (lower part of the Namhae-po). Heavy rain, terrigenous suspended clay with fresh water from neighboring agricultural land, and severe high air temperature during summer could be thought as detrimental causes of spat and adult mortality in Namhae-po tidal flat. We suggested that the growth of shellfish in the tidal flat was effected by the various environmental conditions, so an improvement in the cultured method was needed.