Browse > Article

Effect of Copper toxicant on Suspended and Attached Growth Nitrifying Bacteria  

Kim, Keum-Yong (Department of Environmental Engineering, Chungbuk National University)
Paek, Joo-Heon (Department of Environmental Engineering, Chungbuk National University)
Lee, Sang-Ill (Department of Environmental Engineering, Chungbuk National University)
Publication Information
Journal of Korean Society of Environmental Engineers / v.31, no.10, 2009 , pp. 855-864 More about this Journal
Abstract
The effect of toxicant on the inhibition of nitrification was investigated, using concentrated nitrifying bacteria of both attached and suspended growth. This nitrifying organism was originally obtained from the activated sludge of sewage treatment plant and cultivated for more than three months. The object of this experiment is to determine the effect of the specific surface area and the growth condition of nitrifying bacteria on toxicity of heavy metal. The results of this study were as follows. The specific surface area of both attached and suspended growth of nitrifying organism was proven to be a major factors in determining the inhibition of nitrification of heavy metal such as $Cu^{++}$ion. When the condition of attachment and detachment was compared in an experiment using attached growth nitrifier, the effect on toxicant was 1.12 times less in attached condition than in detached condition for Nitrosomonas, and 1.09 times less for Nitrobacter. In case of suspended growth nitrifier, the effect on toxicant was 1.46 times less in non-ground condition than in ground condition for Nitrosomonas, and 1.35 times less for Nitrobacter. Also, similar results were obtained in a set of experiments, without adding nitrite to the substrate. In an experiment that compared attached condition using attached growth nitrifier with detached condition using attached growth nitrifier, the effect on toxicant was 1.83 times less in attached condition than in detached one for Nitrosomonas, and 1.78 times less for Nitrobacter. In case of suspended growth nitrifier, the effect on toxicant was 1.27 times less in non-ground condition than in ground condition for Nitrosomonas, and 1.32 times less for Nitrobacter.
Keywords
Attached growth organism; Suspended growth organism; Specific surface area; Toxicant;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Dangcong et al., P. Dangcong, N. Bernet, J. Delgenes and R. Moletta, 'Effects of oxygen supply methods on the performance of a sequencing batch reactor for high ammonium nitrification,' Water Environ. Res. 72, 195-200(2000)   DOI   ScienceOn
2 Bitton, G., 'Bacterial and biochemical tests for assessing chemical toxicity in the aquatic environment,' A review, Crit. Rev. Environ. Control, 13, 51-67(1983)   DOI   ScienceOn
3 Bitton, G., Wastewater Microbiology, John Wiley & Sons, INC., New York, NY, 89-198,(1994)
4 Painter, H. A. and Loveless, J. E. 'Effect of temperature and pH value on the growth-rate constants of nitrifying bacteria in the activated-sludge precess', Water Res., 17(3), 237-248 (1983)   DOI   ScienceOn
5 APHA-AWWA-WPC.F., Standard Methods for Examination of Water and Wastewater (21st ed.), Am. Public Health Ass, Washington DC, U.S.A. (2005)
6 Koopman B. and Bitton G., Toxicity screening in wastewater systems. In: Toxicity testing Using Microorganisms Dutka B.J.and Bitton G.(eds.), CRC Press, Boca Raton, Fla, USA. 101-132(1986)
7 Skinner F. A. and Walker N., 'Growth of Nitrosomonas europaea in batch and continuous culture', Arch. Microbiol, 38, 339-349(1961)
8 Herbert H. P. Fang, Li-Chong Xu, Kwong-Yu Chan, 'Effects of toxic metals and chemicals on biofilm and biocorrosion,' Water Res., 36(19), 4709-4716(2002)   DOI   ScienceOn
9 원성연, '질산화 반응에 미치는 환경인자의 영향' 충북대학교, 박사학위논문(1999)
10 Yakup K. 'The impact of toxicity of metals on the activity of ureolytic mixed culture during the precipitation of calcium', J. Hazard. Mat., 163(2-3), 1063-1067(2009)   DOI   PUBMED   ScienceOn
11 Beckman, W. J., 'Design and Operation of Combined Carbon Oxidation Nitrification Activated Sludge Plant', J. Wat. Pollut. Control. Fed. 44, 1916(1972)
12 Hu et al., Z. Hu, K. Chandran, D. Grasso and B. F. Smets, 'Effect of nickel and cadmium speciation on nitrification inhibition,' Environ. Sci. Technol. 36, 3074-3078(2002)   DOI   ScienceOn
13 Skinner, F. A. and Walker, N., 'Growth of Nitrosomonas europaea in Bath and Continuos Culture,' Arch. Microbiol., 38, 339(1961)
14 임정훈, 우혜진, 전병희, 고주형, 이상일, 김창원, 'MBBR에서 부하에 따른 생물막내 미생물종, 개체수 및 산소농도 분포', 대한환경공학회, 24(11), 2009-2018(2002)
15 Loveless J. E. and Painter H. A., 'The influence of metal ion concentration and pH value on the growth of a Nitrosomonas strain isolated from activated sludge'. J.Gen. Microbiol., 52, 1-14(1968)   DOI
16 Prakasam,T. B. S. and Loehr, R. C., 'Microbial Nitrification and denitrification in concentrated wastes', Water Res., 6, 859-869(1972)   DOI   ScienceOn
17 U. S. Environmental protection Agency(EPA), Manual nitrogen control, EPA/625/R-93/010, office of water, washington, D.C., (1995)