• Title/Summary/Keyword: Surface science

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Development of Calcification-Resistant Bovine Pericardium with $PEO-SO_3$ (I) - An implantation study of bovine pericardium at aorta and pulmonary artery in canine model - ($PEO-SO_3$를 이용한 항석회화 조직첨포의 개발 (I) - 잡견을 이용한 대동맥과 폐동맥 이식 실험연구 -)

  • Kim, Hyoung-Mook;Baek, Man-Jong;Sun, Kyung;Kim, Kwang-Taik;Lee, In-Sung;Kim, Hark-Jei;Lee, Won-Kyu;Park, Ki-Dong
    • Journal of Chest Surgery
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    • v.31 no.10
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    • pp.919-923
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    • 1998
  • Background: Calcific degeneration is unavoidable in either homo- or heterografts implanted in the human body. We have developed a calcification-resistant cardiovascular tissue patch using a novel technique of anticalcification. Materials and methods: Fresh bovine pericardium was harvested at the slaughter house and transfered to the laboratory in Hank's solution. After trimming and fixing the pericardium, it was embedded in 4$^{\circ}C$ 0.65% glutaraldehyde for a week and then washed by phosphate-buffered saline(PBS) of pH 7.4. This prepared pericardium was then stored in 2.5% sulphonated polyethyleneoxide(PEO-SO3) solution for 2 days at room temperature and reversed by 4$^{\circ}C$ NaBH4 solution for 16 hours. To evaluate the calcification-resistance of surface modified bovine pericardium with PEO-SO3, either glutaraldehyde- treated(GA group, n=4) or PEO-SO3-treated pericardial patch(PEO-SO3 group, n=4) was implanted into adult mongrel dog to reconstruct the main pulmonary artery and the descending aorta using a partial clamp technique. After 1 month follow-up, the implanted patches were retrieved to evaluate the pathologic findings and the content of calcium and phosphorous. Results: The PEO-SO3 group showed substantially less retraction and significantly less calcium deposition than the GA group in both aortic(7.10$\pm$1.05 vs. 13.81$\pm$2.33 mg/g of dried tissue) and pulmonary positions(1.55$\pm$0.29 vs. 6.72$\pm$0.70 mg/g)(p<0.01). Phosphorous contents were also less in the PEO-SO3 group than the GA group significantly, 8.11$\pm$1.07 mg/g vs. 19.33$\pm$4.31 mg/g in the aortic and 2.58$\pm$0.40 vs. 12.60$\pm$3.40 mg/g in thepulmonary position(p<0.01). Conclusions: These findings suggest that PEO-SO3 modified bovine pericardium is highly calcification-resistant but further study is needed to evaluate the long-term biological safety and compatibility of the prosthesis.

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A Short Composting Method by the Single Phase Composter for the Production of Oyster Mushroom (느타리버섯 배지 제조기를 이용한 배지의 제조 연구)

  • Lee, Ho-Yong;Shin, Chang-Yup;Lee, Young-Keun;Chang, Hwa-Hyoung;Min, Bong-Hee
    • The Korean Journal of Mycology
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    • v.27 no.1 s.88
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    • pp.10-14
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    • 1999
  • A single phase composter was constructed by modifying the conventional mixer of sawdust for the cultivation of oyster mushroom Pleurotus ostreatus. The machine was designed on the basis of 3-phase-1 system which was controlled in prewetting, pasteurization and fermentation processes. In composting 200 kg of straw and cotton waste in the machine, it took 20 minutes in prewetting step and also to hours at $65^{\circ}C$ in pasteurization process. Postfermentation by aerothermophiles was completed by treating the compost at $45^{\circ}C-50^{\circ}C$ for 48 hours which was shorten 24 hours from the conventional method. In the postfermentation at high temperature, forced aeration and/or vigorous mixing process(es) played a great role in the improvement of spawn quality. The growth of mycelium of oyster mushroom was excellent in the culture combinated with 3 parts of surface inoculation and 7 parts of mechanical mixing.

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Structural Characteristics and Physical Properties of Wild Silk Fibres; Antheraea pernyi and Antheraea yamamai (야잠사의 구조특성 및 물리적 성질)

  • 권해용;박영환
    • Journal of Sericultural and Entomological Science
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    • v.36 no.2
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    • pp.138-146
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    • 1994
  • The structural characteristics of Antheraea yamamai and Antheraea pernyi silk were investigated by using x-ray diffraction method, IR spectroscopy and polarizing microscopy. The amino acid composition, fiber density, thermal decomposition temperature and glass transition temperature were also measured for relating these physical properties to the structure in comparison with those of Bombyx mori silk fiber. There was no significant structural difference between A. yamamai and A. pernyi silk fiber on an examination of x-ray diffraction curve and IR spectrum. Both of these wild silk fibers showed double diffraction peaks at the Bragg angle 2Θ16.7˚ and 20.5˚by x-ray diffraction analysis as well as IR absorption peaks for the bending vibration of specific groups related to ala-ala amino acid sequence. On the other hand, the x-ray diffraction curve and IR spectrum of Bombyx mori silk fiber are different from those of wild silk fibers, indicating different crystal structure as well as amino acid sequences. It showed under the polarizing microscope examination that the birefringence and optical orientation factor of wild silk fibers are much lower than those of B. mori silk. Also, the surface of degummed wild silk fibers was characterized by the longitudinal stripes of microfibrils in the direction of fiber axies. The amino acid composition, which is strongly related to the fine structure and properties, was not significantly different between these two wild silk fibers. However, the alanine content was somewhat less and polar amino acid content more for A. yamamai. As a result of fiber density measurement, the specific gravities of B. mori, A. pernyi and A. yamamai were 1.355~1.356, 1.308~1.311, 1.265~1.301g/㎤ in the order, respectively. The calculated crystallinity(%) was 64% for B. mori and 51~52% for wild silk fibers, which showed same trend by IR method in spite of somewhat higher value. The thermal decomposition behaviour was examined by DSC and TGA, showing that the degradation temperature was in the order of B mori, A. prernyi and A. yamamai at around 350$^{\circ}C$. It was also observed by TGA that the decomposition seems to proceed step by step according to their specific regions in the fiber structure, resulting the difference in their thermal stabilities. The glass transition temperature was turned out to be 220$^{\circ}C$ for B. mori, 240$^{\circ}C$ A. yamamai and 255$^{\circ}C$ A. pernyi by the dynamic mechanical analysis. It is expected that the chemical properties are affected by the dynamic mechanical behavior in accordance with their structural characters.

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Effect of Aster scaber and Ixeris dentata on Contractility and Vasodilation of Cardiovascula and Endothelial Cell in Hyperlipidemic Rat (참취 및 씀바귀 첨가식이가 고지혈증 흰쥐의 심혈관 수축과 이완 및 혈관내피세포에 미치는 영향)

  • Lim, Sang-Sun;Lee, Jong-Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.26 no.2
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    • pp.300-307
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    • 1997
  • The effects of Aster scaber and Ixeris dentata on cadiovascular system in hyperlipidemic rats were examined. Five groups of thirty Sprague Dawley rats were fed with the diet contained 1% cholesterol, 0.25% sodium cholate, 10% coconut oil and 5% lard(control group) for 4 weeks. Each experimental diet group was added with 5% plant powder or extract of the 5% plant powder by dry weight. Contractile or relaxation responses in the isolated artria and thoracic aortae were measured and the morphological changes of the aortic endotherium from the rats fed the experimental diet were inspected. In response to isoproterenol, the number of right atrial spontaneous beat was significantly lower in Cham chyi powder group$(PP_{1})$ and Sumbagui powder group$(PP_{2})$ than control at $10^(-8)M$ concentration. The contraction forces by injection of phenylephrine and calcium in isolated thoracic aorta were significantly low in each experimental groups compared with the control. The relaxation rates by acetylcholine represented comparatively higher value in $PP_{1}$ than control. The morphological changes of endothelial cell surface was a little in $PP_{1}$ and $PP_{2}$ compared with control, while the damages were considerably advanced in Cham chyi and Sumgbagui extract diet group$(PE_{1},\;PE_{2})$.

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A Study on the Frequency of Occurrence of the Aortic Dissection using CT (CT 검사에서 대동맥박리(aortic dissection)의 발생빈도에 관한 고찰)

  • Dong, Kyung-Rae;Choi, Sung-Kwan;Jang, Young-Ill;Ro, Sang-Ho
    • Journal of radiological science and technology
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    • v.31 no.2
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    • pp.115-121
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    • 2008
  • Purpose: Aortic Dissection is very dangerous, prognostic disease, which the bloodstream flow out of the true lumen of the aorta by the bursting of aortic intima resulting in a rapid dissociation of inner and outer layer from the media. It is difficult to diagnose aortic dissection clinically by normal X-ray. This study was to investigate the occurrence frequency by age and number of patients who are identified to be aortic dissection by CT (Computed Tomography) scan. Materials and methods: We investigated the trend of yearly fluctuation, gender, age, and department of clinical research of the 112 patients who conducted CT scan in C- University Hospital for two years from January 2005 to December 2006. The MIP and SSD which reconstructed CT image and the VRT image were obtained for the accurate observation. The result was investigated by comparing normal X-ray and CT scan. Results and Conclusion: 1. The yearly check of 112 patients conducted CT scan showed 37 people (41.9%) in 2005, and it was increased to 65 (58.1%) in 2006 by 1.4 times. 2. The gender distribution of patients given a CT scan showed 45 males (40.1%), and female 67 (59.9 %). The aortic dissection patients were 9 (20%) out of 45 males, 21 (31.3%) out of 67 females and women were 1.6 times more than men. Women are also 1.5 times more than men in the number of examinee. 3. The age distribution of patient's who conducted CT scan revealed that there was no patient under 30 years old while 88.3% of all patients were through 41 to 80 years old. The higher the age was, the higher the occurrence of aortic dissection was. The difference in the occurrence frequency of age was statistically significant (p<0.01). 4. The departments that requested CT scan were the emergency department 46 (41.1%), circulatory internal medicine 37 (33.0%), chest surgery 13 (11.6%), and others 6 (14.3%). The combined ratio of emergency medicine and circulatory internal medicine was 74.1% of all. The results show that the aortic dissection is a very dangerous disease whose patients visit mainly via the emergency room. 5. The aortic dissection patients had normal X-ray readings in 22 (73.3%) out of 30, and only 8 (26.7 percent) are abnormal in the X-ray diagnosis. Therefore, the CT scan needs to be enforced in order to assess accurately the disease of aortic dissection.

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Development of the Holocene Sediments in Gamak Bay of the South Sea, Korea (남해 가막만의 현생퇴적층 발달특성)

  • Kim, So Ra;Lee, Gwang Soo;Choi, Dong Lim;Kim, Dae Choul;Lee, Tae Hee;Seo, Young Kyo
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.19 no.2
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    • pp.131-146
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    • 2014
  • High-resolution seismic profiles coupled with sediment sampling were analyzed to investigate the acoustic characters and distribution patterns of the late Holocene sediments in Gamak Bay of the South Sea, Korea. The mean grain size of surficial sediment lies around $6.3{\sim}9.7{\Phi}$. Sediments in the bay consist of silt and clay with progressive decrease toward the inner bay. The seismic sedimentary sequence overlying the acoustic basement can be divided into two sedimentary units (GB I and II) by a prominent mid-reflector (Maximum Flooding Surface; MFS). The acoustic basement occurs at the depth between 20 m and 40 m below the sea-level and deepens gradually southward. The GB I, mostly occupying the channel-fill, is characterized by reflection-free seismic facies. It can be formed as late Transgressive System Tract (TST), interpreted tidal environment deposits. MFS appears at the depth of about 15~28 m below the sea-level and is well defined by even and continuous reflectors on the seismic profile. The GB II overlying MFS is composed of acoustically transparent to semitransparent and parallel internal reflectors. GB II is interpreted as the Highstand System Tract (HST) probably deposited during the last 6,000 yrs when the sea level was close to the present level. Especially, it is though that the GB II was subdivided into two layers (GB II-a and II-b) by a HST-reflector and this was classified by wind, sea water flux, and tidal current.

Studies on the Absorption Capacity of Phosphorus of Korean Top-soils (우리나라 표층토(表層土)의 인산흡수력(燐酸吸收力)에 관(關)한 연구(硏究))

  • Park, C.S.;Han, K.H.;Lim, S.K.;Lee, J.H.
    • Korean Journal of Soil Science and Fertilizer
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    • v.2 no.1
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    • pp.1-13
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    • 1969
  • A total of 129 surface(upto 30 cm depth) soil samples were selected from the profile samples collected during reconnaissance soil survey in 1967, for the determination of phosphorus absorption co-efficient. The distribution range for each soil association has been established. The physicochemical factors affecting the phosphorus absorption coefficient have also been examined. The following general conclusions can be drown: 1. In general, the phosphorus absorption coefficient of the soil association of presently arable land are lower than the soils which are not in cultivation. 2. The higher the cation exchange capacity of soils, the higher is the phosphorus absorption coefficient. The factors governing phosphorus absorption coefficient in various soil associations are as follows: Parent Material Soil Association Governing Factor Fluvio marine Low Humic Gley Fluvio marine Alluvial Complex Narrow valley Siliceo mafic materials Red-yellow podzolic Redish Siliceo mafic materials Brown Lateritic Clay content Siliceous crystalline materials Lithosols C.E.C. & Clay content Alluvium Low Humic Alluvium Gley Alluvial Organic matter Siliceous crystalline materials Red-Yellow Podzolic Organic matter and clay content 4. The relation between phosphorus absorption coefficient determined by $(NH_4)_2HPO_4(y)$ and by the P 700 ppm $NaH_2PO_4(x)$ is $Y=2.716X+37(r=0.96^{**})$ which shows highly significant positive correlation and linear regression.

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Changes in Evapotranspiration and Soil Water Status to Vinyl mulching (Vinyl mulching에 의(依)한 증발산량(蒸發散量) 및 토양수분(土壤水分) 변화(變化)에 관(關)한 연구(硏究))

  • Eom, Ki-Cheol;Son, Eung-Ryong;Ryu, Kwan-Shig
    • Korean Journal of Soil Science and Fertilizer
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    • v.23 no.2
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    • pp.100-106
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    • 1990
  • Changes in soil water potential and soil water content, evapotranspiration and water use efficiency during red-pepper cultivation were investigated under different mulching conditions(Non-mulching, Transparent vinyl and Black vinyl mulching) in a sandy loam soil for 1982 & 1983. The soil water potential up to 20cm depth for transparent vinyl mulching were higher than those of non-mulching condition until the rain free days lasted 25 days, and more than 6 weeks for black vinyl mulching. Root zone water content up to 10cm, 20cm, 30cm, and 50cm for transparent vinyl mulching were lower than those of non-mulching condition after the rain free days lasted more than 2weeks, 3weeks, 5weeks and 6weeks, respectivery. Soil water depletion of deep soil depth were more than that of surface zone for transparent vinyl mulching as the rain free days continued, whereas there was no difference to soil depth for non-mulching condition. Evapotranspiration rates under transparent vinyl mulching condition were higher in dry season but lower in wet season than those of non-mulching condition. Total evapotranspiration during growing season of mulching with transparent or black vinyl was not siginficantly different from that of non-mulching condition, whereas water use efficiency was increased by vinyl mulching with not only whole mulching but also top mulching.

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Changes in Inorganic Phosphorus Fractions in P Accumulated Plastic Film House Soils under Different Cropping Condition (인산축적 시설 재배지 토양에서 작물재배에 따른 무기태 인의 형태별 함량 변화)

  • Jin, Sheng-Ai;Lee, Sang-Mo;Choi, Woo-Jung;Yoo, Sun-Ho
    • Korean Journal of Soil Science and Fertilizer
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    • v.34 no.4
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    • pp.255-264
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    • 2001
  • This study was conducted to investigate changes in inorganic phosphorus fractions in phosphorus accumulated plastic film house soils under cropping condition. Pot experiment was conducted with surface soils taken from plastic film house fields cultivated for 3, 8 and 16 years. Phosphorus fertilizer was applied at the rates of 0 (P0), $100kg\;ha^{-1}$ (recommended application rate. P1) and $200kg\;ha^{-1}$ (P2) as fused phosphate. Crops were grown sequentially in the order of corn-Chinese cabbage-Chinese cabbage-corn for 3 years. The content of easily soluble-P fraction was decreased with cropping. There were significantly positive relationship not only between the contents of easily soluble-P and the amounts of soil available P ($r=0.839^{**}{\sim}0.952^{**}$ for Bray-1 P, $r=0.895^{**}{\sim}0.967^{**}$ for Lancaster P, and $r=0.491^{**}{\sim}0.821^{**}$ for Olsen P), but also between the amounts of P absorbed by plants and the amounts of easily soluble P decreased ($r=0.644^{**}{\sim}0.822^{**}$). The decrease of easily soluble-P during cropping period could be described by a first-order reaction. The number of cropping times needed to decrease the content of easily soluble-P to an index level of $0.2mg\;kg^{-1}$, which is commonly reported as the desired concentration for soil P, was predicted in the range of 26~33 cropping times by using the equations. Regardless of P fertilization, the proportion of Al-P to total P was little varied during cropping period, but the proportion of Fe-P to total P increased with cropping. Although the content of Ca-P was high before cropping, the proportion of Ca-P to total P was increased with cropping. The proportion of reductant soluble-P to total P was little varied for P fertilizer treatment but was increased for no P fertilizer treatment. The residual-P was decreased during cropping period due to the absorption by crops and the conversion to other inorganic P fractions.

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Status and Changes in Chemical Properties of Paddy Soil in Korea (우리나라 논토양의 화학성 현황과 변동)

  • Kang, Seong-Soo;Roh, Ahn-Sung;Choi, Seung-Chul;Kim, Young-Sang;Kim, Hyun-Ju;Choi, Moon-Tae;Ahn, Byung-Koo;Kim, Hyun-Woo;Kim, Hee-Kwon;Park, Jun-Hong;Lee, Young-Han;Yang, Sang-Ho;Ryu, Jong-Soo;Jang, Young-Sun;Kim, Myeong-Sook;Sonn, Yeon-Kyu;Lee, Chang-Hoon;Ha, Sang-Gun;Lee, Deok-Bae;Kim, Yoo-Hak
    • Korean Journal of Soil Science and Fertilizer
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    • v.45 no.6
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    • pp.968-972
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    • 2012
  • Soil chemical properties of agricultural soils in Korea were investigated at four-years interval in order of paddy, plastic film house, upland, and orchard soils since 1999. Paddy soil samples were taken from the surface 15 cm at 4,047, 2,010, 2,110 and 2,110 sites in all provinces of South Korea in 1999, 2003, 2007 and 2010, respectively. Soil chemical properties in Korea except Jeju province were measured. Soil pH and exchangeable calcium and available silicate contents increased with increasing the application rate of silicate fertilizer and with decreasing its application interval. Soil organic matter content also increased from $22.0g\;kg^{-1}$ in 1999 to $26.0g\;kg^{-1}$ in 2011. Average concentration of available phosphate in 2011 was higher than the upper limit of its optimal range for rice cultivation. However, exchangeable magnesium and available silicate contents were below the lower limit of their optimal ranges, which were 80% and 92% of them, respectively.