• Title/Summary/Keyword: Surface science

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Difference of Absorption and Anatomical Responses to Protoporphyrinogen Oxidase-Inhibiting Herbicides in Wheat and Barley (Protoporphyrinogen Oxidase 저해형 제초제에 대한 밀과 보리의 흡수 및 해부하적 차이)

  • 구자옥;국용인
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.1
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    • pp.68-78
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    • 1997
  • Laboratory and greenhouse studies were conducted to determine differential sensitivities on absorption of $^{14}$ C-oxyfluorfen and the anatomical responses in wheat and barley to protoporphyrinogen oxidase-inhibiting herbicides [oxyfluorfen (2-chloro-1- (3-ethoxy -nitrophen-oxy)-4-(trifluoromethyl) benzene, acifluorfen(5-[2-chloro-4-(trifluoromethyl) phenoxy]-2-nitro-benzoic acid), bifenox(methyl-5-(2, 4-dichlorophenoxy)-2-nitrobenzoate) and oxadiazon(5-tert-butyl-3-(2, 4-dichloro-5-isopropoxyphenyl)-1, 3, 4-oxadiazol-2-one)]. I$_{50}$ value of the tolerant wheat cultivars to oxyfluorfen was about 10$^{-4}$ , whereas that of the susceptible barley cultivars was about 10$^{-6}$ M, showing significant difference between the two groups. When foliage were applied with acifluorfen, bifenox or oxadiazon, the oxyfluorfen-tolerant wheat showed less decreased in shoot fresh weight and chlorophyll content than the susceptible barley. Also, when soil-applied with these herbicides test plants showed similar tendency in foliar application. Electrolyte leakage from the tissue treated with these compounds was the more influenced in the barley than the wheat. Malondialdehyde(MDA) production as index of lipid peroxidation was greater in the barley than the wheat by treatment of these compounds. Therefore, the differential sensitivities of wheat and barley to protoporphyrinogen oxidaseinhibiting herbicides was showed by our greenhouse and in vitro experiment. The absorption rates of $^{14}$ C-oxyfluorfen were higher in the barley than the wheat. And this tendency was showed appararitly difference by increase of treatment durations. After the oxfluorfen and oxadiazon treatment, the tolerant wheat did not show the structural damage in leaf surface, but the susceptible barley was damaged in the leaf waxy layer. However, the acifluorfen and bifenox treatment showed no difference between wheat and barley. The anatomical changes by these compounds treatment were not observed in the tolerant wheat but epidermal cell and mesophyll cell were highly broken in the susceptible barley.

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Spatial Distribution of Rice Root under Long-term Chemical and Manure Fertilization in Paddy (화학비료 및 희비 장기시용에 따른 벼 뿌리 분포 특성)

  • 전원태;박창영;조영손;박기도;윤을수;강위금;박성태;최진용
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.48 no.6
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    • pp.484-489
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    • 2003
  • It is well known that root distribution of rice is a crucial factor for nutrient absorbtion and affect by soil fertility management. However, the findings on root distribution are limited due to laborious and tedious work. The characteristics of root distribution were investigated in long-term fertilizer experiment plots that were established in paddy soil, a fine silty family of typic Hal-paqueps (Pyeongtaeg series) in 1967. fertilizer experiment plots of no fertilizer, compost, NPK and NPK+compost plot have been maintained consistently for the past thirty six year and Npk+silicate plot for the past twenty two years. In NPK plot, 150kg N (urea), 100kg -$\textrm{P}_2\textrm{O}_5$ (fused phosphate) and 100kg $\textrm{K}_2\textrm{O}$(potassium chloride) per hectare have been applied. For NPK+silicate plot, 500kg $\textrm{Si}\textrm{O}_2$ (silicate) was applied in addition to fertilizer in NPK plot. For the compost plot, 10,000kg rice straw compost per hectare were applied. Root samples were taken from the positions of hill-center (below hill) and mid-point of four adjacent rice hills at heading stage by cylinder monolith (CM) method. The soil cores were sampled 20cm depth from the soil surface and partitioned four into layers at an interval of 5cm. The soil particles surrounding roots were washed out with tap water, Length and weight of the roots in each soil layer were measured and root length density (RLD), root weight density (RWD), specific root length(SRL) and rooting depth index (RDI) were calculated. Total root length was measured by intersection method. Plant height, tiller and shoot dry weight were the highest in NPK+compost plot. But RLD of hill-center soil cores was the highest in no-fertilizer plots. In the soil cores from mid-point position of four adjacent hills, RLD at 15-20cm soil depth was higher in compost plot than NPK plot. RLD in compost plots showed even distribution compared to those in chemical- fertilizer plots. RWD was the highest in the NPK+compost plot. SRL was the lowest in the NPK+silicate plot. RDI was the highest in the compost plot. Also, in this experiment it was found that the distribution of roots was closely related to the physical properties of the soil as affected by fertilization management.

Assessment of Water Pollution by Discharge of Abandoned Mines (휴폐광산 지역에서 유출되는 하천수의 오염도 평가)

  • Kim Hee-Joung;Yang Jay-E.;Ok Yong-Sik;Lee Jai-Young;Park Byung-Kil;Kong Sung-Ho;Jun Sang-Ho
    • Journal of Soil and Groundwater Environment
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    • v.10 no.5
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    • pp.25-36
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    • 2005
  • Several metalliferous and coal mines, including Myungjin, Seojin and Okdong located at the upper watershed of Okdong stream, were abandoned or closed since 1988 due to the mining industry promotion policy. Thus these disposed an enormous amount of mining wastes without a proper treatment facilities, resulting in water pollution in the downstream areas. Acid mine drainage (AMD) and waste water effluents from the closed coal mines were very strongly acidic showing pH ranges of 2.7 to 4.5 and had a high level of Total Dissolved Solids (TDS) showing the ranges of 1,030 to 1,947 mg/L. Also heavy metal concentrations in these samples such as Fe, Cu, Cd and anion such as sulfate were very high. Concentrations of water soluble heavy metals in the Okdong streams were in the orders of Fe>Al>Mn>Zn>Cu>Pb>Cd, indicating Fe from the AMD and waste water effluents contributed greatly to the quality of water and soil in the lower watershed of Okdong stream. Copper concentrations in the effluents from the tile drainage of mine tailings dams were highest during the raining season. Water Pollution Index (WPI) of the surface water at the upper stream of Okdong river where AMD of the abandoned coal mines was flowed into main stream were in the ranges of 16.3 to 47.1. On the other hand, those at the mid stream where effluents from tailings dams and coal mines flowed into main stream were in the WPI ranges of 10.6 to 19.5. However, those at the lower stream were ranged from 10.6 to 14.9. These results indicated that mining wastes such as AMD and effluents from the closed mines were the major source to water pollution at the Okdong stream areas.

Strength Evaluation of Sin91e-Radius Total Knee Replacement (TKR) (인공무릎관절의 단축법위 회전시 근력정가)

  • Wan, Jin-Young;Sub, Kwak-Yi
    • Journal of Life Science
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    • v.14 no.3
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    • pp.484-489
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    • 2004
  • Artificial joint replacement is one of the major surgical advances of the 21th century. The primary purpose of a TKA (Total Knee Arthroplasty) is to restore normal knee Auction. Therefore, ideally, a TKA should: (a) maintain the natural leverage of the knee joint muscles to ensure generating adequate knee muscle moments to accomplish daily tasks such as rising from a chair or climbing stairs;(b) allow the same range of motion as an complete knee; and (c) provide adequate knee joint stability. Four individuals (2 peoples after surgery one year and 2 peoples after surgery three years) participated in this study. All they were prescreened for health and functional status by the same surgeon who performed the operations. Two days of accommodation practice occurred prior to the actual strength testing. The isometric strength (KIN-COM III) of the quadriceps and hamstring were measured at 60$^\circ$ and 30$^\circ$ of knee flexion, respectively. During isokinetic concentric testing, the range of motion was between 10$^\circ$ to 80$^\circ$ of knee flexion (stand-to-sit) and extension (sit-to-stand). for a given test, the trial exhibiting maximum torque was analyzed. A 16-channel MYOPACTM EMG system (Run Technologies, Inc.) was used to collect the differential input surface electromyographic (EMG) signals of the vastus medialis (VM), vastus lateralis(VL), rectus femoris (RF) during sit-to-stand and stand-to-sit tests. Disposable electrodes (Blue SensorTM, Medicotest, Inc.) were used to collect the EMG signals. The results were as follows; 1. Less maximum concentric (16% and 21% less for 1 yew man and 3 years mm, respectively) and isometric (12% and 29%, respectively) quadriceps torque for both participants. 2.14% less maximum hamstrings concentric torque for 1 year man but 16% greater torque for 3 years mm. However, 1 year man had similar hamstring isometric peak torque for both knees. 3. Less quadriceps co-contraction by 1 year man except for the VM at 10$^\circ$-20$^\circ$ and 30$^\circ$-50$^\circ$ range of knee flexion.

Monitoring of Quality Characteristics of Chungkookjang Products during Storage for Shelf-life Establishment (청국장 제품의 유통기한 설정을 위한 저장중의 품질 특성 monitoring)

  • Kim, Dong-Myung;Kim, Seong-Ho;Lee, Jin-Man;Kim, Ji-Eun;Kang, Sun-Chul
    • Applied Biological Chemistry
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    • v.48 no.2
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    • pp.132-139
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    • 2005
  • The major obstacle in the popularization of Chungkookjang is the short shelf-life of $2{\sim}3$ months and some problems concerning storage including the growth of molds even in the products even within shelf-life. To solve these problems we conducted a research to improve its storage by using the vacuumed packaging and sanitary method through seed culture, innoculation and sterilization. For the optimization of storage time, temperature and sterilization temperature, we measured viable cell numbers of bacteria and fungi, amount of gas outbreak and contents of amino type nitrogen and monitored these experimental results by response surface methodology of SAS program, so that we could observe the quality changes of Chungkookjang during shelf-life. Especially fungi, which are the biggest troublemaker in Chungkookjang shelf-life, couldn't be detected from the generally and vacuum-packed samples; also, viable cell numbers were highly influenced by sterilization temperature and in vacuum-packed samples. In the case of vacuum-packed samples, amount of gas outbreak was highly influenced by sterilization temperature of its storage conditions and it was higher in generally packed samples as compared to vacuum-packed samples even at any storage conditions. The changes of pH in generally and vacuum-packed samples were highly influenced by the storage temperature. As the temperatures of storage and sterilization were higher and the storage time was longer, so the amount of gas outbreak was accordingly lower. These results showed that amino type nitrogen contents in generally and vacuum-packed samples were systematically influenced by the temperature, storage time and sterilization temperature. Also the result showed that the change of amino type nitrogen contents during storage was less in vacuum-packed samples than in general ones. Based on the above results, we can produce Chungkookjang products with extended shelf-life of as far as 6 months without any quality change using sanitary manufacturing method, vacuumed packaging condition, sterilization in $70^{\circ}C$ for 60 minutes and storage under $10^{\circ}C$ during shelf-life. According to this research, we have the possibility to greatly increase the goods value of Chungkookjang by developing the manufacture processing and packaging.

Chemical Composition Characteristics of Dustfall in Nakdong River Area (낙동강 하류역 강하먼지의 화학적 조성 특성)

  • Jeon, Byung-Il;Hwang, Yong-Sik
    • Journal of the Korean earth science society
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    • v.25 no.6
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    • pp.428-442
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    • 2004
  • Dustfall samples were collected by the modified American dust jar (bulk type) at 5 sampling sites in the Nakdong river area from lune 2002 to May 2003. Nineteen chemical species (Al, Ca, Cd, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, Pb, Si, V, Zn, $Cl^-$, $NO_3^-$, $SO_4^{2-}$, and $NH_4^+$) were analyzed via the combination of ICP/AES, AAS, IC and UV. The purposes of this study were to qualitatively evaluate the chemical composition of dustfalls by examining their regional and seasonal distribution patterns. Computation of the enrichment factor showed that well-defined anthropogenic sources, particularly in Pb were found in the order Gamjeondong (industrial area), Wondong, Silla University, Samrangiin and Mulgum. The seasonal mean of soil contribution showed its highest value (16.3%) during the winter with an annual mean of 11.2%. The concentration ratio of [$SO_4^{2-}/NO_3^-$] was found to be highest (5.12) during the winter, while the lowest ratio value (3.30) was seen during the all. fall, Also regional equivalent ratios of [$SO_4^{2-}/NO_3^-$] were found in the order: Silla University (6.78), Gamjeondong (4.98), Mulgum (3.95), Wondong (3.85), and Samrangjin (2.87). Seasonal distribution of water soluble components for total dustfall were found in the order: spring (71.6%), summer (61.2%), fall (49.2%) and winter (48.6%) with a mean ratio of 57.6%. Regional contribution of sea salts of water soluble ions were found in the order: Silla university (34.5%), Gamjeondong (28.3%), Wondong (17.3%), Samrangiin (17.2%) and Mulgum (13.8%), the total mean contribution rate was 22.1%. As for the chemical composition of dustftll on the lower Nakdong river, there is a decreased influence of sea salt and artificial anthropogenic sources and increased influence of soil particle inland. Also, the total amount of deposition on the lower Nakdong river has decreased, with the river's surface serving as a confounding factor in resuspending dusts.

The Character of Distribution of Solar Radiation in Mongolia based on Meteorological Satellite Data (위성자료를 이용한 몽골의 일사량 분포 특성)

  • Jee, Joon-Bum;Jeon, Sang-Hee;Choi, Young-Jean;Lee, Seung-Woo;Park, Young-San;Lee, Kyu-Tae
    • Journal of the Korean earth science society
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    • v.33 no.2
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    • pp.139-147
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    • 2012
  • Mongolia's solar-meteorological resources map has been developed using satellite data and reanalysis data. Solar radiation was calculated using solar radiation model, in which the input data were satellite data from SRTM, TERA, AQUA, AURA and MTSAT-1R satellites and the reanalysis data from NCEP/NCAR. The calculated results are validated by the DSWRF (Downward Short-Wave Radiation Flux) from NCEP/NCAR reanalysis. Mongolia is composed of mountainous region in the western area and desert or semi-arid region in middle and southern parts of the country. South-central area comprises inside the continent with a clear day and less rainfall, and irradiation is higher than other regions on the same latitude. The western mountain region is reached a lot of solar energy due to high elevation but the area is covered with snow (high albedo) throughout the year. The snow cover is a cause of false detection from the cloud detection algorithm of satellite data. Eventually clearness index and solar radiation are underestimated. And southern region has high total precipitable water and aerosol optical depth, but high solar radiation reaches the surface as it is located on the relatively lower latitude. When calculated solar radiation is validated by DSWRF from NCEP/NCAR reanalysis, monthly mean solar radiation is 547.59 MJ which is approximately 2.89 MJ higher than DSWRF. The correlation coefficient between calculation and reanalysis data is 0.99 and the RMSE (Root Mean Square Error) is 6.17 MJ. It turned out to be highest correlation (r=0.94) in October, and lowest correlation (r=0.62) in March considering the error of cloud detection with melting and yellow sand.

Improvement and Validation of Convective Rainfall Rate Retrieved from Visible and Infrared Image Bands of the COMS Satellite (COMS 위성의 가시 및 적외 영상 채널로부터 복원된 대류운의 강우강도 향상과 검증)

  • Moon, Yun Seob;Lee, Kangyeol
    • Journal of the Korean earth science society
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    • v.37 no.7
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    • pp.420-433
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    • 2016
  • The purpose of this study is to improve the calibration matrixes of 2-D and 3-D convective rainfall rates (CRR) using the brightness temperature of the infrared $10.8{\mu}m$ channel (IR), the difference of brightness temperatures between infrared $10.8{\mu}m$ and vapor $6.7{\mu}m$ channels (IR-WV), and the normalized reflectance of the visible channel (VIS) from the COMS satellite and rainfall rate from the weather radar for the period of 75 rainy days from April 22, 2011 to October 22, 2011 in Korea. Especially, the rainfall rate data of the weather radar are used to validate the new 2-D and 3-DCRR calibration matrixes suitable for the Korean peninsula for the period of 24 rainy days in 2011. The 2D and 3D calibration matrixes provide the basic and maximum CRR values ($mm\;h^{-1}$) by multiplying the rain probability matrix, which is calculated by using the number of rainy and no-rainy pixels with associated 2-D (IR, IR-WV) and 3-D (IR, IR-WV, VIS) matrixes, by the mean and maximum rainfall rate matrixes, respectively, which is calculated by dividing the accumulated rainfall rate by the number of rainy pixels and by the product of the maximum rain rate for the calibration period by the number of rain occurrences. Finally, new 2-D and 3-D CRR calibration matrixes are obtained experimentally from the regression analysis of both basic and maximum rainfall rate matrixes. As a result, an area of rainfall rate more than 10 mm/h is magnified in the new ones as well as CRR is shown in lower class ranges in matrixes between IR brightness temperature and IR-WV brightness temperature difference than the existing ones. Accuracy and categorical statistics are computed for the data of CRR events occurred during the given period. The mean error (ME), mean absolute error (MAE), and root mean squire error (RMSE) in new 2-D and 3-D CRR calibrations led to smaller than in the existing ones, where false alarm ratio had decreased, probability of detection had increased a bit, and critical success index scores had improved. To take into account the strong rainfall rate in the weather events such as thunderstorms and typhoon, a moisture correction factor is corrected. This factor is defined as the product of the total precipitable waterby the relative humidity (PW RH), a mean value between surface and 500 hPa level, obtained from a numerical model or the COMS retrieval data. In this study, when the IR cloud top brightness temperature is lower than 210 K and the relative humidity is greater than 40%, the moisture correction factor is empirically scaled from 1.0 to 2.0 basing on PW RH values. Consequently, in applying to this factor in new 2D and 2D CRR calibrations, the ME, MAE, and RMSE are smaller than the new ones.

The expression of MMP-1, -8, and -13 mRNA in the periodontal ligament of rats during tooth movement with cortical punching (백서의 치아이동 시 피질골 천공이 치주조직의 MMP-1, -8, -13 mRNA의 발현에 미치는 영향)

  • Gwack, Choon;Kim, Seong-Sik;Park, Soo-Byung;Son, Woo-Sung;Kim, Yong-Deok;Jun, Eun-Sook;Park, Mi-Hwa
    • The korean journal of orthodontics
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    • v.38 no.3
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    • pp.187-201
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    • 2008
  • Objective: The aim of this study was to determine whether cortical punching stimulates the expression of matrix metalloproteinase-1, -8, and -13 in orthodontic tooth movement in rats. Methods: A total of 32 male sprague-dawley rats at 15 weeks old were divided into two groups of 16 rats each, to form the tooth movement with cortical punching (TMC) group and tooth movement only (TM) group. A total of 20 gm of orthodontic force was applied to rat incisors to cause experimental tooth movement. Cortical punching was performed on the palatal side near the central incisor with a 1.0 mm width microscrew in the TMC group. The duration of tooth movement was 1, 4, 7, and 14 days. Results: Measurements of the mRNA expression were selected as the means to determine the identification of expression of MMP-1, -8, and -13. In the TMC group, the expression of collagen type I was greater than that of the TM group from day 4 to day 14. Expression of TIMP-1 in the TM group was greater than that of the TMC group in the pressure side of PDL and alveolar bone cell at day 4. In the TMC group, TIMP-1 was expressed at the osteoclast, but not at the tooth surface of the TM group at day 14, Maximum induction of the mRNA of MMP-1 was observed on day 4 in the TMC group, but it was observed on day 7 in the TM group. MMP-8 mRNA of the TMC group was twice greater than that of the TM group at f days. In the TMC group, maximum induction of MMP-13 mRNA was observed on day 1. Conclusions: These findings suggested that cortical punching can stimulate remodeling of PDL and alveolar bone connective tissues during experimental orthodontic tooth movement in rats.

Effect of Radiation Dose for Radiotherapy on Ovarian Follicle Atresia in Rat (치료 방사선량이 쥐의 난포 퇴축에 미치는 영향)

  • Lee, Won-Jeong;Seon, Jong-Ryul;Yoo, Se-Jong;Ahn, Bong-Seon
    • Journal of Radiation Protection and Research
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    • v.37 no.4
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    • pp.208-212
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    • 2012
  • In previous studies, ovarian follicle in rat has been used a higher radiation dose than that for cancer radiotherapy in clinical practice. The aim of this study was to evaluate the effect of radiation dose used for cancer radiotherapy on ovarian follicle atresia in rat. Mice of 4-week-old female were whole body irradiated with 2 cGy or 2 Gy (Mevatron 67, Siemens, Germany) and sacrificed by cervical dislocation. Ovaries were collected at 24 hours after irradiation to observe the degree of follicular atresia. Ovaries were fixed in neutral formaldehyde solution for 24 hours and embedded with paraffin. Cutted in $5{\mu}m$ thickness with microtome and stained with hematoxylin and eosin (H&E) and TUNEL immunohistochemical stain, and examined histologically under a light microscope. All data were presented as mean ${\pm}SD$, calculating the ratio of normal or atretic follicles to total ovarian follicles. Statistical analysis was performed by the Mann Whitney test using the SPSS ver 19.0. Ratio of atretic to total follicles of 2 Gy group was significantly higher than control or 2 cGy groups (p<0.05). Ratio of normal to total follicles of 2 Gy group was significantly lower than control group in preantral follicle (64.0 vs. 87.7, p=0.027). Ratio of normal to total follicles of 2 cGy group was significantly increased more than control or 2 Gy groups in antral follicle, and there were no significant difference between control and 2 Gy groups (p=0.522). Radiation dose of 2 Gy for cancer radiotherapy have a significant effect on ovarian follicle atresia in rat.