• Journal of the Society of Naval Architects of Korea
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• v.46 no.6
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• pp.650-658
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• 2009

Two dimensional sloshing phenomena in regularly excited liquid cargo tank are numerically simulated with finite difference method. Navier-Stokes equations and continuity equation are computed for this study. The free-surface is determined every time step satisfying kinematic boundary condition using marker-density method. And the exciting force is treated by adding the acceleration of the tank to source term. The results are compared with other existing experiment results. And the comparison results show a good agreement. The sloshing phenomena in the tank of the 138K LNG carrier in sway motion is simulated with present calculation methods in low filling level. To find the relations between impact pressure and excitation condition, the calculations are performed in various amplitudes and periods. The averaged maximum pressures are compared each other.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.10
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• pp.4939-4941
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• 2012

Background: The serum level of soluble CD30 (sCD30) is known to be increased with several lymphomas and to correlate with prognosis. Primary effusion lymphoma (PEL) is a highly aggressive malignant lymphoma with poor prognosis, but the existence and significance of sCD30 in PEL have not yet been investigated in detail. Objectives: Since the membrane type of CD30 is frequently expressed on the surface of PEL cells, we compared the expression of the membrane type of CD30 and the production of sCD30 among PEL cell lines as well as other lymphomas. Methods: The expression of surface CD30 in various lymphoma cell lines was analyzed with flow cytometry ans sCD30 was quantified by ELISA. Results: Both surface and sCD30 were detected on PEL cell lines as well as on Hodgkin's lymphoma and adult T-cell leukemia/lymphoma cell lines. Surface CD30 and sCD30 levels of each cell lines correlated with each other. Conclusion: The serum level of sCD30 appear to be a useful biological tumor marker for the diagnosis and management of CD30-positive PEL.

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2003.10a
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• pp.198-203
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• 2003

Unsteady two-dimensional nonlinear waves which are generated by the numerical wavemaker of plunging type are numerically simulated using a finite difference method in rectangular grid system. Two-dimensional Navier-Stokes equations and the continuity equation are used for the computations. Irregular leg lengths and stars are employed near the boundaries of body and free surface to satisfy the boundary conditions. Marker-density function method is adopted for the simulation of wave breaking phenomena, and the computations are carried out with various wave amplitudes and two section shapes of wavemaker. The computation results are compared with other existing computational and experimental results, and the agreement between the experimental data and the computation results is good.

• The Journal of Korean Society for Radiation Therapy
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• v.33
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• pp.109-116
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• 2021

Purpose: The purpose of this study is to evaluate the usefulness of Surface-Guided Patient Setup by comparing the patient positioning accuracy when image-guided radiation therapy was used for Markerless patients(unmarked on the skin) using Surface-Guided Patient Setup and Marker patients(marked on the skin) using Laser-Based Patient Setup. Materials And Methods: The position error during IGRT was compared between a Markerless patient initially set up with SGPS using an optical surface scanning system using three cameras and a Marker patient initially set up with LBPS that aligns the laser with the marker drawn on the patient's skin. Both SGPS and LBPS were performed on 20 prostate cancer patients and 10 Stereotactic Radiation Surgery patients, respectively, and SGPS was performed on an additional 60 breast cancer patients. All were performed IGRT using CBCT or OBI. Position error of 6 degrees of freedom was obtained using Auto-Matching System, and comparison and analysis were performed using Offline-Review in the treatment planning system. Result: The difference between the root mean square (RMS) of SGPS and LBPS in prostate cancer patients was Vrt -0.02cm, Log -0.02cm, Lat 0.01cm, Pit -0.01°, Rol -0.01°, Rtn -0.01°, SRS patients was Vrt 0.02cm, Log -0.05cm, Lat 0.00cm, Pit -0.30°, Rol -0.15°, Rtn -0.33°. there was no significant difference between the two regions. According to the IGRT standard of breast cancer patients, RMS was Vrt 0.26, Log 0.21, Lat 0.15, Pit 0.81, Rol 0.49, Rtn 0.59. Conclusion:. As a result of this study, the position error value of SGPS compared to LBPS did not show a significant difference between prostate cancer patients and SRS patients. In the case of additionally performed SGPS breast cancer patients, the position error value was not large based on IGRT. Therefore, it is considered that it will be useful to replace LBPS with SGPS, which has the great advantage of not requiring patient skin marking..

• 대한치과보철학회:학술대회논문집
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• 2004.11a
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• pp.52-52
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• 2004

• The Journal of Advanced Prosthodontics
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• v.7 no.6
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• pp.496-505
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• 2015

PURPOSE. To determine the effect of fibronectin (FN)-conjugated, microgrooved titanium (Ti) on osteoblast differentiation and gene expression in human bone marrow-derived mesenchymal stem cells (MSCs). MATERIALS AND METHODS. Photolithography was used to fabricate the microgrooved Ti, and amine functionalization (silanization) was used to immobilize fibronectin on the titanium surfaces. Osteoblast differentiation and osteoblast marker gene expression were analyzed by means of alkaline phosphatase activity assay, extracellular calcium deposition assay, and quantitative real-time PCR. RESULTS. The conjugation of fibronectin on Ti significantly increased osteoblast differentiation in MSCs compared with non-conjugated Ti substrates. On the extracellular calcium deposition assays of MSCs at 21 days, an approximately two-fold increase in calcium concentration was observed on the etched 60-${\mu}m$-wide/10-${\mu}m$-deep microgrooved surface with fibronectin (E60/10FN) compared with the same surface without fibronectin (E60/10), and a more than four-fold increase in calcium concentration was observed on E60/10FN compared with the non-etched control (NE0) and etched control (E0) surfaces. Through a series of analyses to determine the expression of osteoblast marker genes, a significant increase in all the marker genes except type I collagen ${\alpha}1$ mRNA was seen with E60/10FN more than with any of the other groups, as compared with NE0. CONCLUSION. The FN-conjugated, microgrooved Ti substrate can provide an effective surface to promote osteoblast differentiation and osteoblast marker gene expression in MSCs.

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2004.11a
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• pp.90-95
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• 2004

During the past 50 years methods for predicting wave overtopping of coastal structures have coastal structures have continuously been developed Wave overtopping is one of the most important processes for the design of seawalls. The term 'wave overtopping' is used here to refer to the processes where waves hit a sloping structure run up the slope and, if the crest level of the slope is lower than the highest run up level, overtop the structure. Wave overtopping is dependent on the processes associated with breaking wave. The Numerical model is based on Navier-Stokes equation and Marker-Density Function of method for nonlinear free-surface flow by Miyata & Park(1995). The influence of how the slopes of seawalls, wave type and crest freeboard affect overtopping discharges has been investigated. The research of study using the new development nonlinear free-surface flow numerical model SOLA-VOF are presented.

• Journal of the Society of Naval Architects of Korea
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• v.51 no.5
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• pp.419-428
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• 2014

This paper presents a numerical simulation method for the flow around advancing ships in regular waves by using a rectilinear grid system. Because the grid lines do not consist with body surface in the rectilinear grid system, the body geometries are defined by the interaction points of those grid lines and the body surface. For the satisfaction of body boundary conditions, no-slip and divergence free conditions are imposed on the body surface and body boundary cells, respectively. Meanwhile, free surface is defined with the modified marker density method. The pressure on the free surface is determined to make the pressure gradient terms of the governing equations continuous, and the velocity around the free surface is calculated with the pressure on the free surface. To validate the present numerical method, a vortex induced vibration (VIV) phenomenon and flows around an advancing Wigley III ship model in various regular waves are simulated, and the results are compared with existing and corresponding research data. Also, to check the applicability to practical ship model, flows around KRISO Container Ship (KCS) model advancing in calm water are numerically simulated. On the simulations, the trim and the sinkage are set free to compare the running attitude with some other experimental data. Moreover, flows around the KCS model in regular waves are also simulated.

• Molecules and Cells
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• v.43 no.4
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• pp.384-396
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• 2020

Breast cancer is one of the most common life-threatening malignancies and the top cause of cancer deaths in women. Although many conventional therapies exist for its treatment, breast cancer still has many handicaps to overcome. Cancer stem cells (CSCs) are a well-known cause of tumor recurrences due to the ability of CSCs for self-renewal and differentiation into cell subpopulations, similar to stem cells. To fully treat breast cancer, a strategy for the treatment of both cancer cells and CSCs is required. However, current strategies for the eradication of CSCs are non-specific and have low efficacy. Therefore, surface biomarkers to selectively treat CSCs need to be developed. Here, 34 out of 641 surface biomarkers on CSCs were identified by proteomic analysis between the human breast adenocarcinoma cell line MCF-7 and MCF-7-derived CSCs. Among them, carcinoembryonic antigen-related cell adhesion molecules 6 (CEACAM6 or CD66c), a member of the CEA family, was selected as a novel biomarker on the CSC surface. This biomarker was then experimentally validated and evaluated for use as a CSC-specific marker. Its biological effects were assessed by treating breast cancer stem cells (BCSCs) with short hairpin (sh)-RNA under oxidative cellular conditions. This study is the first to evaluate the biological function of CD66c as a novel biomarker on the surface of CSCs. This marker is available as a moiety for use in the development of targeted therapeutic agents against CSCs.

• Journal of Mechanical Science and Technology
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• v.15 no.3
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• pp.384-391
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• 2001

The aerodynamic effects of a 3-dimensional Wing in Ground Effect (WIG) which moves above the free surface has been numerically investigated via finite difference techniques. The air flow field around a WIG is analyzed by a Marker & Cell (MAC) based method, and the interactions between WIG and the free surface are studied by the pressure distributions on the free surface. Waves are generated by the surface pressure distribution, and a Navier-Stokes solver has been employed, to include the nonlinearities in the free surface conditions. The pressure values Cp and lift/drag ratio are reviewed by changing the height/chord ratio. In the present computations a NACA0012 airfoil with a span/chord ratio of 3.0 are treated. Through computational results, it is confirmed that the free surface can be treated as a rigid wavy wall.