• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.277.2-277.2
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• 2013

Petri dishes and glass slides have been widely used as general substrates for in vitro mammalian cell cultures due to their culture viability, optical transparency, experimental convenience, and relatively low cost. Despite the aforementioned benefit, however, the flat two-dimensional substrates exhibit limited capability in terms of realistically mimicking cellular polarization, intercellular interaction, and differentiation in the non-physiological culture environment. Here, we report a protocol of culturing embryonic rat hippocampal neurons on the electro-spun polymeric network and the results from examination of neuronal cell behavior and network formation on this culture platform. A combinatorial method of laser-scanning confocal fluorescence microscopy and live-cell imaging technique was employed to track axonal outgrowth and synaptic connectivity of the neuronal cells deposited on this model culture environment. The present microfiber-based scaffold supports the prolonged viability of three-dimensionally-formed neuronal networks and their microscopic geometric parameters (i.e., microfiber diameter) strongly influence the axonal outgrowth and synaptic connection pattern. These results implies that electro-spun fiber scaffolds with fine control over surface chemistry and nano/microscopic geometry may be used as an economic and general platform for three-dimensional mammalian culture systems, particularly, neuronal lineage and other network forming cell lines.

• Transactions of Materials Processing
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• v.25 no.6
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• pp.379-389
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• 2016

As a stator for steam turbine diaphragm, hollow-type nozzle stator to substitute for conventional solid one is introduced in this study. This hollowed stator can be separated into two parts such as upper and lower plates with large and curved surface area. This study focuses on thick plate forming process for the upper plate of the hollow-partitioned nozzle stator. First, to reduce forming defects such as under-cut and localized thinning of the deformed plate, and to avoid tool interruption between forming punch and lower die, tool design including the position determination of forming surfaces is performed. Uni-axial tensile tests are carried out using SUS409L steel plate with initial thickness of 5.00mm, and plastic strain ratio (r-value) is also obtained. Due to the asymmetric curved configuration of the upper plate, it is hard to adopt a series of blank holder or draw-bead, so the initial plate during this thick plate forming experiences unstable and non-uniform contact. To easy this forming difficulty and find suitable tool geometry without sliding behavior of the workpiece in the die cavity, two geometric parameters with respect to each shoulder angle of the lower die and the upper punch are adopted. FE models with consideration of 21 combinations for the geometric parameters are built-up, and numerical simulations are performed. From the simulated and predicted results, it is shown that the geometric parameter combinations with ($30^{\circ}$, $90^{\circ}$) and ($45^{\circ}$, $90^{\circ}$) for the shoulder angle of the lower die and the upper punch are suitably applied to this upper plate forming of the hollow-partitioned nozzle stator used for the turbine diaphragm.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.38 no.2
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• pp.185-192
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• 2014

Planetary gear design is complex because it involves a combination of discrete variables such as module, integer variables such as the number of teeth, and continuous variables such as face width and aspect ratio. Thus, an optimum design technique is needed. In this study, we applied a genetic algorithm to the design optimization of a planetary gear. In this algorithm, tooth root strength and surface durability are assessed with fundamental variables such as the number of teeth, module, pressure angle, and face width. With the help of this technique, gear designers could reduce trial and error in the initial design stages, thus cutting the time required for planetary gear design.

• Proceedings of the KACD Conference
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• 2003.11a
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• pp.547-547
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• 2003

The aim of this study was to compare the effects of three Ni-Ti instruments on leaning ability by evaluating the volumetric and morphological changes in the apical 6mm of the root canals before and after preparation, using three-dimensionally reconstructed root canals of extracted human teeth. Forty-five teeth were used in this study. They were opened the chambers and removed the all pulp remnants ultrasonically. Subsequently, the canal wall was coated with silver paste and prepared using ProTaper, ProFile and GT rotary files according to the manufacturers instructions. Before and after root canal preparation, all the specimens were scanned with micro computed tomography and examined the differences in dentine volume removed, canal straightening, the proportion of the unchanged area and canal transportation. Quantitative analysis revealed that instrumentation increased in canal volume ranging between 0.081 and $1.866{\;}\textrm{mm}^3$. On average, the large apical preparation produced by ProTaper demonstrated smaller proportions of unchanged surface areas compared to the two other instruments in small canals. But in large canals like maxillary central incisor, the preparation of ProTaper instruments was not enough. ProTaper instrument was tended to increase more in canal volume as compared with the other two instruments but unchanged area was no significant difference. These results showed that three instruments had similar preparation ability and micro computed tomography in combination with the coated wall of root canal using silver paste is a nondestructive and valuable tool to study root canal geometry and changes after preparations in detail.

• Restorative Dentistry and Endodontics
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• v.36 no.1
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• pp.1-11
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• 2011

Introduction: The aim of this paper is to discuss the mechanical and geometric features of Nickel-titanium (NiTi) rotary files and its clinical effects. NiTi rotary files have been introduced to the markets with their own geometries and claims that they have better ability for the root canal shaping than their competitors. The contents of this paper include the (possible) interrelationship between the geometries of NiTi file (eg. tip, taper, helical angle, etc) and clinical performance of the files as follows; - Fracture modes of NiTi rotary files - Non-cutting guiding tip and glide path - Taper and clinical effects - Cross-sectional area and clinical effects - Heat treatments and surface characteristics - Screw-in effect and preservation of root dentin integrity - Designs for reducing screw-in effect Conclusions: Based on the reviewed contents, clinicians may have an advice to use various brands of NiTi rotary instruments regarding their advantages which would fit for clinical situation.

• Polymer(Korea)
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• v.35 no.6
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• pp.531-536
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• 2011

Homopolymer distribution in block copolymer/homopolymer blends was investigated as a function of homopolymer concentration and homopolymer molecular weight. The deuterated poly(methyl methacrylate) or polystyrene was blended with a deuterated polystyrene-poly(methyl methacrylate) diblock copolymer up to a concentration of 20 wt%. Samples were characterized by small-angle X-ray scattering (SAXS), neutron reflectivity and transmission electron microscopy. The block copolymer with a thin-film geometry formed alternating lamellar microdomains oriented parallel to the substrate surface. By adding the homopolymer, the microdomain structure was significantly disturbed. As a consequence, a poorly ordered morphology appeared when the homopolymer concentration exceeded 15 wt%. Increasing the homopolymer concentration and/or the homopolymer molecular weight caused the microdomains to swell less uniformly, resulting in segregation of the homopolymer toward the middle of the microdomains.

• Smart Structures and Systems
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• v.18 no.3
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• pp.485-500
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• 2016

Stay cables in some cable-stayed bridges suffer large amplitude vibrations under the simultaneous occurrence of rain and wind. This phenomenon is called rain-wind-induced vibration (RWIV). The upper rivulet oscillating circumferentially on the inclined cable surface plays an important role in this phenomenon. However, its small size and high sensitivity to wind flow make measuring rivulet size and its movement challenging. Moreover, the distribution of the rivulet along the entire cable has not been measured. This paper applies the videogrammetric technique to measure the movement and geometry dimension of the upper rivulet along the entire cable during RWIV. A cable model is tested in an open-jet wind tunnel with artificial rain. RWIV is successfully reproduced. Only one digital video camera is employed and installed on the cable during the experiment. The camera records video clips of the upper rivulet and cable movements. The video clips are then transferred into a series of images, from which the positions of the cable and the upper rivulet at each time instant are identified by image processing. The thickness of the upper rivulet is also estimated. The oscillation amplitude, equilibrium position, and dominant frequency of the rivulet are presented. The relationship between cable and rivulet variations is also investigated. Results demonstrate that this non-contact, non-intrusive measurement method has good resolution and is cost effective.

• Nuclear Engineering and Technology
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• v.53 no.4
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• pp.1289-1296
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• 2021

In this study, an electron-scattering device was fabricated to practically use the ultra-high dose rate electron beams for the FLASH preclinical research in Dongnam Institute of Radiological and Medical Sciences. The Dongnam Institute of Radiological and Medical Sciences has been involved in the investigation of linear accelerators for preclinical research and has recently implemented FLASH electron beams. To determine the geometry of the scattering device for the FLASH preclinical research with a 6-MeV linear accelerator, the Monte Carlo N-particle transport code was exploited. By employing the fabricated scattering device, the off-axis and depth dose distributions were measured with radiochromic films. The generated mean energy of electron beams via the scattering device was 4.3 MeV, and the symmetry and flatness of the off-axis dose distribution were 0.11% and 2.33%, respectively. Finally, the doses per pulse were obtained as a function of the source to surface distance (SSD); the measured dose per pulse varied from 4.0 to 0.2 Gy/pulse at an SSD range of 20-90 cm. At an SSD of 30 cm with a 100-Hz repetition rate, the dose rate was 180 Gy/s, which is sufficient for the preclinical FLASH studies.

• Nuclear Engineering and Technology
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• v.52 no.12
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• pp.2949-2957
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• 2020

Fatigue crack growth model has been developed for dissimilar metal weld joints of a piping component under cyclic loading, where in the crack is located at the center of the weld in the circumferential direction. The fracture parameter, Stress Intensity Factor (SIF) has been computed by using principle of superposition as K_H + K_M. K_H is evaluated by assuming that, the complete specimen is made of the material containing the notch location. In second stage, the stress field ahead of the crack tip, accounting for the strength mismatch, the applied load and geometry has been characterized to evaluate SIF (K_M). For each incremental crack depth, stress field ahead of the crack tip has been quantified by using J-integral (elastic), mismatch ratio, plastic interaction factor and stress parallel to the crack surface. The associated constants for evaluation of K_M have been computed by using the quantified stress field with respect to the distance from the crack tip. Net SIF (K_H + K_M) computed, has been used for the crack growth analysis and remaining life prediction by Paris crack growth model. To validate the model, SIF and remaining life has been predicted for a pipe made up of (i) SA312 Type 304LN austenitic stainless steel and SA508 Gr. 3 Cl. 1. Low alloy carbon steel (ii) welded SA312 Type 304LN austenitic stainless-steel pipe. From the studies, it is observed that the model could predict the remaining life of DMWJ piping components with a maximum difference of 15% compared to experimental observations.

• Journal of the Society of Naval Architects of Korea
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• v.56 no.1
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• pp.11-22
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• 2019

In this paper, a Rankine source method is applied and validated to analyze the hydrodynamic response of a three-dimensional floating structure in the frequency domain. The boundary value problems for radiation and diffraction problem are solved by using a desingularized indirect boundary integral equation method (DIBIEM). The DIBIEM is simpler and faster than conventional methods based on the numerical surface integration of Green's function because the singularities of Green's function are located outside of fluid regions. In case of floating structure with complex geometry, it is difficult to desingularize the singularities of Green's function consistently. Therefore a mixed approach is carried out in this study. The mixed approach is partially desingularized except singularities of the body. Wave drift loads are calculated by the middle-field formulation method that is mathematically simple and has fast convergence. In order to validate the accuracy of the developed program, various numerical simulations are carried out and these results are analyzed and compared with previously published calculations and experiments.