• Mass Spectrometry Letters
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• v.13 no.3
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• pp.58-83
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• 2022

We developed analytical methods using high performance chromatography (HPLC) and liquid chromatography tandem mass spectrometry (LC-MS/MS) for the simultaneous determination of 80 unapproved compounds in dietary supplements. The target compounds for analysis were unapproved ingredients (e.g., pharmaceuticals) that have potential adverse effects on consumers owing to accidental misuse, overuse, and interaction with other medication in dietary supplement. Two analytical methods were tested to identify the optimal validation results according to AOAC guideline. As a result, limit of quantification (LOQ) was 0.14-0.5 ㎍ mL^-1; linearity (r²) was ≥ 0.99; accuracy (expressed as recovery) was 78.9-114%; precision (relative standard deviation) was ≤ 4.28% in the HPLC method. In the LC-MS/MS method, LOQ was 0.01-2 ng mL^-1, linearity (r²) was ≥0.98, accuracy was 71.7-119%; precision was ≤ 12.5%. The developed methods were applied to 51 dietary supplements collected from 2019 to 2021 through MFDS alert system. Based on our previous monitoring study, major compounds were icariin, sibutramine, yohimbine, sildenafil, tadalafil, sennosides (A, B), cascarosides (A, B, C, D), and phenolphthalein. In this study, we re-analyzed samples of detected compounds, and evaluated the statistical difference using Bland-Altman analysis to compare two analytical approaches between HPLC and LC-MS/MS. These results showed a good agreement between two methods that can be used to monitor the unapproved ingredients in dietary supplements. The developed two methods are complementarily suitable for monitoring the adulteration of 80 unapproved compounds in dietary supplements.

• Journal of Bio-Environment Control
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• v.20 no.4
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• pp.269-276
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• 2011

The aim of this study was to evaluate the effect of 3 environment- friendly farming materials on the growth of pepper and tomato seedlings under low level of solar radiation. The pepper and tomato seedlings were separately grown on commercial substrate and non-fertilizer substrate under 30% shading condition, respectively. The microbe-, enzyme-, and amino acid-supplements were used for the foliar application to the seedlings. The application of enzyme- and amino acid-supplements significantly enhanced the growth of tomato and pepper seedlings grown on non-fertilizer substrate, while there was no significant effect on the seedlings grown on commercial substrate. The foliar application of enzyme- and amino acid-supplements reduced the days to flowering by 3 to 4 days and lowered the node order of fruit set in tomato plants grown on non-fertilizer substrate. The results showed that the enzyme- and amino acid-supplements could enhance the seedling growth and lower the node order of fruit set under limited nutritional conditions.

• The Journal of Dong Guk Oriental Medicine
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• v.11
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• pp.41-51
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• 2008

Dietary supplements are used by more than one-half of the adult US population. By contrast, herbal products in Germany are carefully regulated by the same standards as drugs, and efforts are under way to standardize their regulation in the entire European Union. Most herbal users do not inform their physicians that they are taking these supplements, and most physicians do not inquire. Although some herbal products have clinically proven benefits, it is increasingly apparent that many contain potentially toxic substances, particularly in relation to interactions with drugs. Hence, it is essential that practicing physicians develop a working knowledge of herbals-specifically, about claims for their usage and potential or proven efficacies and toxicities-and that they incorporate such knowledge into the evaluation and management of their patients. By contrast, functional foods-integral components of the diet that are understood to contribute added health benefits-are the subject of intense and widespread research in food and nutritional science. Examples include many polyphenolic substances, carotenoids, soy isoflavones, fish oils, and components of nuts that possess antioxidant and other properties that decrease the risk of vascular diseases and cancer. Practicing physicians are advised to stay abreast of these emerging findings in order to best advise their patients on the value of health-promoting diets in disease prevention.

• Proceedings of the Korean Society of Food Science and Nutrition Conference
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• 2004.11a
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• pp.203-208
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• 2004

The Family of Man was a 1955 photographic exhibition that depicted normal people in all walks of life from around the world. The viewer was visually confronted with the similarity of all people. The people of the world are now facing common health challenges as a result of changing lifestyles and increasing healthcare costs. Dietary supplements have the potential to be part of the solution. Dietary supplementation with antioxidant vitamins may be the most effective intervention for preventing the onset of Alzheimer's disease in the rapidly expanding elderly population - and at a very low cost. AIDS is devastating much of the developing world, where few resources are available to treat it victims. Inexpensive multivitamin sup-plements have been demonstrated to slow the progression of AIDS, reduce the death rate, reduce AIDS-related morbidity, improve T cell counts, and reduce viral loads. Careful economic analysis demonstrate that calcium and folic acid supplementation could reduce healthcare costs in the USA by $13.9 billion and $1.3 billion, respectively. Most supplements with therapeutic potential have not been subjected to sufficiently rigorous research to permit unqualified public health recommendations. Carefully directed research is needed to identify the supplements with the greatest potential and to verify their safety and efficacy.

• Mass Spectrometry Letters
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• v.3 no.2
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• pp.50-53
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• 2012

After success of sildenafil for the treatment of erectile dysfunction, a large number of its analogues have been approved from FDA. Recently, the illegal dietary supplements which include sildenafil, vardenafil, tadalafil, or analogues of these drugs as ingredient have been widely distributed. Therefore, the determination of the residue of synthetic phosphodiesterase- 5 (PDE-5) inhibitors in dietary supplements is highly required due to indiscriminate and unintentional overdose caused nausea, chest pains, fainting and irregular heartbeat. In this paper, we report a rapid and sensitive analytical method for the simultaneous determination of nine phosphodiesterase-5 inhibitors by liquid chromatography-high resolution mass spectrometry. The present method was found to be accurate and reproducible with 40 ${\mu}g$/g of the limit of quantification for the nine PDE-5 inhibitors. The developed method can be successfully applied to the analysis of the seven illegal dietary supplements.

• Clinical and Experimental Reproductive Medicine
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• v.16 no.2
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• pp.161-171
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• 1989

The development of 2-cell mouse embryos to the blastocyst stage in vitro has been used as quality control for the culture media and supplements employed for human in vitro fertilization and embryo transfer(IVF-ET). 2-cell mouse embryos were cultured to the blastocyst stage in SECM, Medium 199-Earle's, Ham's F-10 I , Ham's F-10 II , Hoppe & Pitts, MEM and $HT_6$. The protein supplements contained in media were bovine serum albumine, fetal bovine serum and human fetal cord serum. The results were as follows; 1. The successful development was 81.3% in Medium 199-Earle’s, 91.9% in Ham’s F-10 I and 97.1% in $HT_6$. 2. 2-cell mouse embryos developed properly in all supplements but the best development was particularly noted in $HT_6$ media when HFCS was supplied as protein supplement.

• Journal of Nutrition and Health
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• v.26 no.4
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• pp.433-442
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• 1993

Dietary folate intake and serum folate levels were measured in 26 pregnant, 25 lactating, and 17 non-pregnant, non-lactating women. Dietary folate comsumption was estimated by calculating folate intake based on the information obtained from food frequency quesionnaires and serum folate levels were determined microbiologically using Lactobacillus casei. The total folate (from food and supplements) intakes of pregnant and lactating women were 326.9ug and 407.9ug, which was significantly higher than that of the non-pregnant, non-lactating women(139.5ug). However, with regard to food folate intake, there were no differences among the three groups (160ug for pregnant women, 143.4ug for lactating women). Forty-two percent and 36% of the pregnant and lactating subjects, respectively, were found to be taking commercially available nutritional supplements containing folate. The concentrations of folate in these supplements were in the range of 83ug~1, 000ug per tablet. For lactating women, serum folate levels were significantly higher when folate supplements were voluntarily used. The amount of folate intake was positively correlated with the serum folate levels in pregnant women, but not in lactaing women and non-pregnant, non-lactating women. Serum folate levels were negatively correlated with the ages of the pregnant women, and for lactating women, serum folate was positively correlated with their body weights.

• Journal of Korean Medicine for Obesity Research
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• v.18 no.2
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• pp.128-143
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• 2018

Objectives: The purpose of this study is to investigate the efficacy and the mechanisms of dietary supplements in cellulite treatment, and then to provide the theoretical and clinical basis for the cellulite treatment in Korean Medicine. Methods: We searched for articles from Korea, China, and English electronic database (Koreanstudies Information Service System [KISS], National Digital Science Library [NDSL], KMbase, Research Information Sharing Service [RISS], Oriental Medicine Advanced Searching Integrated System [OASIS], National Assembly Library, Korean Traditional Knowledge Portal, Google scholar, PubMed, Scopus, China National Knowledge Infrastructure [CNKI]) until April 2018. We chose clinical trial studies by inclusion criteria through titles, abstracts and articles. Results: A total of 10 studies were selected through search. The experimental group had shown more effective cellulite improvement in 90% of studies. Also, improvement of symptoms related with cellulite like pain, edema, heaviness and increase of skin surface temperature were observed in experimental group. In addition, the density of connective tissues of the dermal layer was increased in experimental group. Conclusions: The use of dietary supplements in cellulite treatment is thought to be effective through mechanisms that antioxidant efficacy, microcirculation improvement, interstitial matrix improvement, diuretic effect, and skin metabolic activity effect.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.5
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• pp.643-650
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• 2009

Two metabolism trials were conducted with 24 wether lambs to investigate the effects of feeding crab meal and other protein supplements on N utilization, digestibility and Ca and P balance in sheep. The lambs (avg. BW, 25 kg) were randomly allotted to eight diets in each of two trials. The supplements were: i) none, negative control (NC); ii) soybean meal (SBM), control; iii) supplement based on industrial byproducts of both plant and animal origin (IPA); iv) experimental supplement based on byproducts of animal origin (ESA); v) hydrolyzed supplement No 4. (HESA); vi) commercial supplement based on animal protein (CS), $Pro-Lak^{(R)}$ vii) crab meal (CM); and viii) urea (U). The supplements supplied 33% of the total dietary N (CP, 9.8%; DM basis). Lambs fed the NC diet had lower (p<0.05) DM and OM digestibility. Lower (p<0.05) apparent absorption of N was recorded for the lambs fed the HESA and NC diets. Sheep fed CM had lower Ca absorption compared to SBM. Highest (p<0.05) P absorption was observed for lambs fed CS and CM and lowest for U and NC diets. Sheep fed CM had higher (p<0.05) total VFA concentration (65.7 ${\mu}mol/ml$), compared to those fed ESA, CS, and NC diets (47.3, 49.8, and 49.5 ${\mu}mol/ml$, respectively). Highest (p<0.05) ruminal $NH_3$ N (29.6 mg/dl) was observed in lambs fed the U diet, while those fed the NC diet had the lowest (p<0.05) average value (7.66 mg/dl). Lambs fed the U diet had the highest (p<0.05) blood urea N (10.67 mg/dl). The present study showed that N utilization of diets supplemented with experimental supplements based on feather meal and blood meal; commercial supplement based on animal protein, $Prolak^{(R)}$ supplement based on plant protein and blood meal; and crab meal are comparable with that of soybean meal.

• Nutrition Research and Practice
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• v.4 no.1
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• pp.69-74
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• 2010

There has been little data on the prevalence of supplement use and the characteristics of the dietary supplement users in the Republic of Korea. This study presents the prevalence and the details of any dietary supplement use and the characteristics of the adults who use dietary supplements in the Republic of Korea. Between May 18 and June 16, 2006, nationwide and population-weighted personal interviews with 6,201 adult aged from 30 to 69 years were conducted and the final sample consisted of 3,000 people with a 49.8% response rate. We examined the prevalence and details of the use of dietary supplements and the characteristics of those who use the dietary supplement among adults. About sixty two percent of adults had taken any dietary supplement during the previous 12-month period in 2006. The most commonly reported dietary supplement was ginseng, followed by multivitamins, glucosamine, probiotics, and vitamin C. Female (versus male), an older age group, a higher family income, those living in metropolitan cities, those with marital experience, those with a higher level of education, and those having medical problems had a greater likelihood of reporting the use of any dietary supplements. The particular relationships differed depending on the type of supplement. The most Korean adults took one more dietary supplement and the dietary supplement users had different demographic and health characteristics compared to those of the nonusers. Research on diet supplements by the medical community is needed in the future.