• Food Engineering Progress
• /
• v.15 no.2
• /
• pp.175-181
• /
• 2011

By using supercritical carbon dioxide fluid, an attempt was made to extract surface-active substances from defatted rice bran. Extraction was carried out according to D-optimal design and results were analyzed by response surface methodology to establish optimum condition. It was found that pressure, temperature and co-solvent (ethanol) influenced in a different extent on the extraction efficiency (i.e., yield and interfacial tension) of surface-active substances. Among them, co-solvent was found to be a major influencing factor, where maximum yield (2.62%) was observed at the highest content (250 g). In addition, it also affected most on the interfacial tension at the oil-water interface but in this case the lowest interfacial tension value (9.51 mN/m) was found when added lowest (50 g). In conclusion, it was estimated that the optimum extraction condition was to be pressure 350bar, temperature $62^{\circ}C$ and co-solvent content 50 g in this study, where extraction yield was 0.69% and interfacial tension to be 10.1 mN/m.

• Journal of the Korean Chemical Society
• /
• v.45 no.2
• /
• pp.149-155
• /
• 2001

A new analytical method of cyanobacterial toxins, i, e, microcysins was deveeloped using supercritical fluid extraction(SFE). The microcystins mcluded in the study are sparsely soluble in neat supercritical fluid CO$_2$ However, the microcystins were successfully extracted with a temary mixture(90% CO$_2$,9.0% methanol 1.0% water) at 40$^{\circ}$C and 250 atm. The SFE method developed in this study has several advantages over solid-phase extraction(SPE) sample preparation for the analysis of microcystins. Sample handling steps are minimized thus reducing possible losses of analytes and saving analysis time. No clean-up steps are employed in this SFE method. Althouhgh many methods have been described for microcystim RR and LR, the method using solid-phase extraction with ODS cartridges is the most commonly used. However, the adsorbing power of ODS caridges for microcystins is weak, so we have attempted to use a more polar CN cartridge, to increase the adsorbing power for microcystins. Lyophilized cells(100mg) were wxtracted with 5% (v/v) acetic acid. The extract was centrifuged and then the supernatant was applied to a CN cartridge. The cartridge which contained microcystins was rinsed with 5 ml of water and 5 ml of 0.5 M acetic acid. followed by 5 ml of 5% acetonitrile in water , and were determined by HPLC. Better recoveries and chromatogram were observed than with ODS cartridge.

• Journal of Life Science
• /
• v.23 no.4
• /
• pp.510-517
• /
• 2013

This study was performed to evaluate the antiobesity effect of supercritical fluid extracts (SFC) and marc methanol extracts (SFM) from Cinnamomum verum in 3T3-L1 preadipocytes. In inducing the differentiation of 3T3-L1 preadipocytes in the presence of an adipogenic cocktail, iso-butylmethylanthine (IBMX), dexamathasone, and insulin, treatment with fraction residue SFC and SFM. SFC significantly reduced the mRNA expression of the transcription factor peroxisome proliferator-activate-dreceptor-${\gamma}$ ($PPAR{\gamma}$), the sterol regulatory-element-binding protein-1c (SREBP1c), and the CCAAT enhancer-binding-protein ${\alpha}$ ($C/EBP{\alpha}$) in a concentration-dependent manner. Moreover, SFC markedly down-regulated acyl-CoA synthetase-1 (ASC1), fatty acid synthesis (FAS), fatty acid transport-1 (FATP1), fatty acid binding protein 4 (FABP4), and perilipin. These findings suggest that SFC may be a potential therapeutic adjunct for obesity by targeting the differentiation of preadipocytes, as well as their functions.

• Korean Journal of Food Science and Technology
• /
• v.37 no.3
• /
• pp.431-437
• /
• 2005

Overall experiments were planned by central composite design, and results were analyzed by response surface methodology (RSM) to determine effects of three independent variables, temperature ($X_{1}$), extraction time ($X_{3}$), and pressure ($X_{3}$), on yield of sesame oil extract (Y). Regression equation model optimized by response surface analysis was: Y (sesame oil) = $-3.89+0.07X_{1}+0.03X_{2}+0.0006X_{3}-0.0007X_{1}^{2}-0.0002X_{2}X_{1}-0.00008X_{2}^{2}+0.000004X_{3}X_{1}+0.0000009X_{3}X_{2}-0.00000009X_{3}^{2}$. According to RSM analysis, optimum extracting conditions of temperature, time, and pressure were $45.89^{\circ}C$, 131.89 min, and 34228.41 kPa, respectively, and statistical maximum yield of sesame oil was 96.27%. Fatty acid composition of sesame oil showed sesame oil extracted by Supereritical Fluid $CO_{2}$ contained lower levels of palmitic, stcaric, and oleic acids and higher levels or palmitoleic and linoleic acids than commercial sesame oil. Commercial and extracted sesame oils were analyzed by electronic nose composed of 12 different metal oxide sensors. Obtained data were interpreted by statistical method of MANOVA. Sensitivities of sensors from electronic nose were analysed by principal component analysis. Proportion of first principal component was 99.92%. All sesame oils showed different odors (p < 0.05).

• Journal of the Korean Society of Combustion
• /
• v.7 no.1
• /
• pp.15-22
• /
• 2002

Flame spread experiments of a fuel droplet array were performed using a microgravity environment. N-decane, 1-octadecene, and the blends (50% : 50% vol.) of these fuels were used and the experiments were conducted at pressures up to 5.0 MPa, which are over the critical pressure of these fuels. Observations of the flame spread phenomenon were conducted for OH radical emission images recorded using a high-speed video camera. The flame spread rates were calculated based on the time history of the spreading forehead of the OH emission images. The flame spread rate of the n-decane droplet-array decreased with pressure and had its minimum at a pressure around half of the critical pressure and then increased again with pressure. It had its maximum at a pressure over the critical pressure and then decreased gradually. The pressure dependence of flame spread rate of 1-octadecene were similar to those of n-decan, but the magnitude of the spread rate was much smaller than that of n-decane. The variation of the flame spread for the blended fuel was similar to that of n-decane in the pressure range from atmospheric pressure to near the critical pressure of the blended fuel. When the pressure increased further, it approached to that of 1-octadecene. Numerically estimated gas-liquid equilibrium states proved that almost all the fuel gas which evaporated from the droplet at ordinary pressure consisted of n-decane whereas near and over the critical pressure, the composition of the fuel gas was almost the same as that of the liquid phase, so that the effects of 1-octadecene on the flame spread rate was significant.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2020.06a
• /
• pp.149-149
• /
• 2020

하천에서 물 흐름이 보와 댐과 같은 수공구조물을 지날 때 일반적으로 흐름상태에 다양하고 급진적인 변화가 발생한다. 특히 흐름이 구조물을 지나면서 사류(supercritical flow)로 변하고 다시 상류(subcritical flow)로 복원되면서 일어나는 도수(hydraulic jump) 현상은 수위의 급변화, 흐름 에너지 소산, 변동성이 강한 압력 분포 등이 특징이다. 이러한 흐름 특성들은 보나 여수로와 같은 수공구조물 자체의 성능뿐만 아니라 이들 수공구조물의 하류에서 발생하는 국부세굴로 인해 구조물의 안정성에 부정적인 영향을 줄 수 있다. 따라서 수공구조물을 설계할 때는 이들 구조물을 통과하는 흐름의 비정상 난류 흐름 특성을 정확하게 해석하여 반영하여야 한다. 이 연구에서는 k-omega SST 난류 모형과 자유수면의 급격한 변동을 해석하기 위한 하이브리드-VOF(hybrid volume of fluid)기법을 이용하여 도수현상을 수치적으로 재현하고자 한다. 기존 CFD(computational fluid Dynamics) 모델링에서는 자유수면 변동의 영향을 고려하기 위해 VOF 기법을 많이 사용하였다. 하지면 전통적인 VOF 기법은 다상흐름(multiphase flow)을 오직 부피분율(volume fraction)의 함수로만 고려하며 모의함으로써 강한 수면변동뿐만 아니라 공기연행(air entrainment)를 동반하는 난류 흐름을 모의하는데는 한계가 있다. 이 연구에서 이용하는 Eulerian 기법인 하이브리드 VOF 기법은 물과 공기의 각 상에 대하여 흐름 특성들을 개별적으로 계산하기 때문에 공기연행을 포함한 급변류 흐름에서 전통적인 VOF 기법보다 적용성이 우수하다. 이와 같은 난류모형과 자유수면 포착기법을 이용하여 3차원 비정상 난류 흐름 수치모형을 구축하여 수공구조물 주변에서 발생하는 강한 공기연행과 난류 특성를 보이는 급변류를 수치적으로 재현한다. 이 연구는 계산된 수치해석 결과를 기존 수리실험 결과와 비교하여 수치모형의 적용성을 평가하고 도수 현상에서 발생하는 독특한 흐름 특성을 제시한다.

• Mass Spectrometry Letters
• /
• v.15 no.1
• /
• pp.40-48
• /
• 2024

Capacity of the analytical/quantitative evaluation techniques to satisfy both qualitative and quantitative considerations for effective extraction of marigold oleoresins/xanthophylls and their potential as anti-mycotic and antioxidant activity was assessed. Accelerated solvent extraction (ASE), Soxhlet extraction (SE), Supercritical fluid extraction (SCFE), Cold extraction (CE), and ultrasonically assisted extraction (USE) techniques were evaluated for extraction of oleoresin/xanthophyll content from Tagetes erecta (Var. Pusa Narangi Genda) with respect to solvent consumption, extraction time, reproducibility, and yield. Followed by the antifungal and antioxidant activity evaluation. The overall yield of Tagetes oleoresin was higher in ASE (64.5 g/kg) followed by SE (57.3 g/kg), USE (50.7 g/kg), SCFE (45.3 g/kg) and CE (31.6 g/kg). The lutein esters represented more than 80% of the constituents. Further, xanthophyll/ lutein content in oleoresin was found to be quite higher in HPLC (r2 = 0.996) analysis than in the AOAC recommended UV spectrophotometer analysis. The oleoresin exhibited moderate antioxidant activity (DPPH assay) and antifungal activity against three phytopathogenic fungi. Based on the various parameters, the reproducibility of ASE was better (0.3-8.0%) than that of SE (0.5-12.9%), SCFE (0.2-9.4%), USE (0.3-12.4%) and CE (0.8-15.3%). ASE with (RSD 1.6%) is preferred being faster, reproducible, uses less solvent, robust and automation allows sequential extraction of the sample in less time.

• 한국생물공학회:학술대회논문집
• /
• 2000.04a
• /
• pp.516-519
• /
• 2000

Generally pancreas consist of lipid, water and protein, digestion enzyme complex (pro-tease, lipase, amylase). The sample used in this work was frozen dry and treated by a semi-batch flow type. In order to develop a supercritical fluid extraction process to rem-ove lipid from the pancreas, experiments were conducted at various operating conditions(pressure range $1500{\sim}2800psi$, temperature range $25{\sim}40^{cdot}C$, particle size$(0.25{\sim}1.0mm$, flow rate $20{\sim}80m{\ell}/min)$. Also cholesterol in the pancreas was removed. The highest extraction efficiency was 2500psi, $35^{\cdot}C$, 0.25mm of pancreas size. The enzyme activity of the pancreas produced from this work showed high value compared with imported pancreas.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
• /
• v.12 no.9
• /
• pp.825-834
• /
• 2000

A heat exchanger analysis is performed to investigate the heating characteristics of cryogenic nitrogen by ambient air for the purpose of cryogenic automotive propulsion. The heat exchanger is a concentric triple-passage for supercritical nitrogen, and the radial fins are attached on the outermost tube for the crossflow of ambient air. The temperature distribution is calculated for the nitrogen along the passage, including the real gas properties of nitrogen, the fluid convections and the conductions through the tube walls and the fins. Since the wall temperature of the outer (ambient side) tube is very low in most cases, a heavy frost can be formed on the surface, affecting the heat exchange performance. By the method of the similarity between the heat and the mass transfer of moist air, the frost growth and the time-dependent effectiveness of the heat exchanger are calculated for various operating conditions. It is concluded that the frost formation can augment the heating of nitrogen during the initial period because of the latent heat, then gradually degrades the heat exchange because of the increased thermal resistance. Practical design issues are discussed for the flow rate of nitrogen, the velocity and humidity of ambient air, and the sizes of the fin.

• Proceedings of the KSME Conference
• /
• 2004.11a
• /
• pp.1323-1328
• /
• 2004

This paper shows the study on the design of the spray-freeze dryer for the production of the pulmonary inhalation powders. Powder production and handling has been an integral part of pharmaceutical processing because of the wide use of oral dosage forms. There are a few commonly used powder preparation methods including mechanical milling, precipitaion, spray drying, freeze drying, and so on. In general, methods available for preparing inhalation powders are limited due to certain inhalation powder's sensitive nature to the processing environments. This is particularly true for preparing dry powder aerosols where the aerodynamic particle size$(<5{\mu}m)$ and the size distribution are pivotal. Supercritical fluid antisolvent and spray freeze drying have recently emerged as promising techniques for producing powders for use in microcapsulation. However, the aerosol applications of these powders are yet to be explored. The purpose of this study is to test the feasibility of using spray freeze-dried pulmonary inhalation powders for aerosolization.