• Proceedings of the Korean Society of Toxicology Conference
• /
• 2001.10a
• /
• pp.147-147
• /
• 2001

A previous study showed that sulfur amino acid deprivation (SAAD) potentiated cytotoxicity induced by arsenic (As) and that activation of ERKl/2, p38 kinase and JNK1 was responsible for the potentiation of As toxicity. In the present study, we found for the first time that deprenyl a selective monoamine oxidase B inhibitor prevented potentiation of As toxicity by SAAD in a dose-dependent manner.(omitted)

• Journal of Plant Biology
• /
• v.18 no.4
• /
• pp.155-160
• /
• 1975

Growth responses of lettuce seeds germinated in aqueous solutions of $SO_2$ were investigated for mode of action studies of this gas. The concentration and pH of $SO_2$ solution greatly affected the growth of germinating seeds. Root growth was more sensitive to $SO_2$ than hypocotyl growth. The sensitivity of total seedling growth lay between root and hypocotyl growth. Growth inhibition of germinating seeds appeared more serioius when $SO_2$ was added during the early stage of germination.

• Archives of Pharmacal Research
• /
• v.22 no.6
• /
• pp.559-564
• /
• 1999

In order to discover new cancer chemopreventive agents from natural or synthetic products, a structurally diverse class of chemopreventive agents was evaluated using in vitro biomarker of inhibition of 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced ornithine decarboxylase (ODC) activity in cultured mouse epidermal 308 (ME)308 cells. As a results, apigenin, benzylisothiocyanate, curcumin, diallyl disulfide, N-(4-hydroxyphenyl)retinamide (4-HPR), menadione, miconazole, nordihydroguaiaretic acid (NDGA) and phenethyl isothiocyanate showed potent inhibitory effects in this process. A chemically diverse group of compounds was included in the evaluation, such as flavonoids, retinoids, isothiocyanates, sulfur-containing compounds and phenolic antioxidant compounds. These data are suggestive to understand the cancer chemopreventive potential mediated by these substances.

• Journal of Microbiology
• /
• v.43 no.4
• /
• pp.375-380
• /
• 2005

Autonomous ultradian metabolic oscillation (T$\simeq$50 min) was detected in an aerobic chemostat culture of Saccharomyces cerevisiae. A pulse injection of GSH (a reduced form of glutathione) into the culture induced a perturbation in metabolic oscillation, with respiratory inhibition caused by $H_2S$ burst pro-duction. As the production of $H_2S$ in the culture was controlled by different amino acids, we attempted to characterize the effects of GSH on amino acid metabolism, particularly with regard to branched chain and sulfur-containing amino acids. During stable metabolic oscillation, concentrations of intra-cellular glutamate, aspartate, threonine, valine, leucine, isoleucine, and cysteine were observed to oscil-late with the same periods of dissolved $O_2$ oscillation, although the oscillation amplitudes and maximal phases were shown to differ. The methionine concentration was stably maintained at 0.05 mM. When GSH (100 $\mu$M) was injected into the culture, cellular levels of branched chain amino acids increased dramatically with continuous $H_2S$production, whereas the cysteine and methionine concentrations were noticeably reduced. These results indicate that GSH-dependent perturbation occurs as the result of the promotion of branched chain amino acid synthesis and an attenuation of cysteine and methionine synthesis, both of which activate the generation of $H_2S$. In a low sulfate medium containing 2.5 mM sulfate, the GSH injections did not result in perturbations of dissolved $O_2$ NAD(P)H redox oscillations without burst $H_2S$ production. This suggests that GSH-dependent perturbation is intimately linked with the metabolism of branched-chain amino acids and $H_2S$ generation, rather than with direct GSH-GSSG redox control.

• Journal of Nutrition and Health
• /
• v.17 no.1
• /
• pp.60-67
• /
• 1984

Inhibiting action of sulfur dioxide $(SO_{2})$ on enzymatic discoloration was investigated with crude enzyme preparations from homogenized tissues or sliced disks of raw potato tubers. $SO_{2}$ appeared to inhibit the formation of dopachrome in a competitive manner. At insufficient concentrations of $SO_{2}, the formation of dopachrome was reinitiated as time elapsed. The present results suggested that $SO_{2}$ would form an additional compounds with certain intermediate during the course of enzymatic browning and delay the enzymatic discoloration. To inhibit the production of dopachrome in sliced disks of raw potato tubers, much higher concentrations of $SO_{2}$ were required than homogenized tissues.

• International Journal of Oral Biology
• /
• v.39 no.1
• /
• pp.35-40
• /
• 2014

Halitosis is caused by consumption of certain foods or drinks and production of volatile sulfur compounds (VSCs) by periodontopathogens. VSCs-related halitosis is not easily removed using mechanical or chemical therapies such as dental floss, plaque control and mouth rinse. Lactobacillus are known to be probiotics and stimulate immune systems of human. Furthermore, L. casei ATCC 334 and L. rhamnosus GG have an effect on protection of dental caries in vitro studies. The aim of this study was to investigate effect of Lactobacillus on halitosis by Fusobacterium nucleatum- and Porphyromonas gingivalis-producing VSCs and to analyze inhibitory mechanism. The periodontopathogens were cultivated in the presence or the absence Lactobacillus, and the level of VSCs was measured by gas chromatograph. For analysis of inhibitory mechanisms, the susceptibility assay of the spent culture medium of Lactobacillus against F. nucleatum and P. gingivalis was investigated. Also, the spent culture medium of Lactobacillus and periodontopathogens were mixed, and the emission of VSCs from the spent culture medium was measured by gas chromatograph. L. casei and L. rhamnosus significantly reduced production of VSCs. L. casei and L. rhamnosus exhibited strong antibacterial activity against F. nucleatum and P. gingivalis. The spent culture medium of L. casei inhibited to emit gaseous hydrogen sulfide, methyl mercaptan and dimethyl sulfide from the spent culture medium of periodontopathogens. However, the spent medium of L. rhamnosus repressed only dimethyl sulfide. L. casei ATCC 334 may improve halitosis by growth inhibition of periodontopathogens and reduction of VSCs emission.

• Korean Journal of Organic Agriculture
• /
• v.24 no.2
• /
• pp.253-262
• /
• 2016

The SS (Bacillus subtilis 10%), WS (Microbial extract 70%), and DS (Sulfur 78%) agents were selected by mycelial growth inhibitory effect test against kiwifruit soft rot pathogen (B. dothidea) with 11 kinds of environment-friendly agricultural materials on PDA medium for 10 days. They showed at 94.2%, 65.2%, 58.9%, respectively. The control value of WS and SS agents were better than DS in storage experiment. It was effective SS and WS single application, DS-WS and WS-SS alternate application in the field trial.

• Korean Journal of Agricultural Science
• /
• v.7 no.1
• /
• pp.38-43
• /
• 1980

Ethylene production and inhibition of ethylene production by Ag ion were investigated in ornamental trees injured by $SO_2$ exposure. Concurrently the sulfur content of leaves was measured to examine the absorption of $SO_2$. The results were summarized as follows: 1. Ethylene production by leaves of Pinus thunbergii and Cedrus deodara showed peak after 2 - day exposures to $SO_2$ when visible injury was not appeared ; whereas in Taxus cuspidata showed continuos increase even after 4 - day exposures and was markedly stimulated at leaves appeared visible injury. The amount of ethylene production by leaves of Cedrus deodara and Taxus cuspidata was higher than those of Pinus thunbergii. 2. Ag ion applied on leaves at concentration of 100 ppm $A_gNO_3$ significantly inhibited ethylene production by leaves of Cedrus deodara either $A_gNO_3$ sprayed before $SO_2$ exposure or after leaves were injured by $SO_2$ exposure. 3. The sulfur content of leaves after 5 - day exposures to $SO_2$ showed higher in Pinus thunbergii and Cedrus deodara than in Taxus cuspidata, but there were no great differences in the sulfur content among untreated species.

• The Korean Journal of Nuclear Medicine
• /
• v.1 no.1
• /
• pp.37-54
• /
• 1967

The changes in histopathology of various organs and growth inhibition of the chick embryos incubated with radioactive sulfur ($^{35}S$) were experimentally studied. The various doses of $^{35}S$ were injected into the yolk sac at different intervals and the weight changes of the embryos were evaluated to determine the growth inhibition rates. The embryos sacrified on various incubation days were used for the study of histopathological changes in organs such as the bone, liver, kidney, gonad, and eye. Following were the results: 1) The weight changes of the $^{35}S$ treated groups were as follows: i. Embryos treated on the 5 th incubation day: No weight changes were noted on the 8th incubation day, however, the growth inhibition rate of 32.1% was noted in the group treated with $50{\mu}C$ and of 38.2% in the group treated with $150{\mu}C$ on the 12th incubation day. The rates were 9.1 and 12.1% on the 15th incubation day, and 6.5 and 10.6% on the 18th incubation day respectively. ii. Embryos treated on the 8th incubation day: The growth inhibition rates on the 12th, 15th and 18th incubation days in the groups treated with $50{\mu}C$ were 20.9, 25.9 and 18.8% and in those treated with $150{\mu}C$ were 20.0, 14.9 and 16.9% respectively. iii. Embryos treated on the 12th incubation day: The growth inhibition rates on the 15th and 18th in the groups treated with $50{\mu}C$ were 13.6 and 21.1% and in those treated with $150{\mu}C$ were 26.7 and 6.5% and in those treated with $250{\mu}C$ were 10.6 and 12.6% respectively. iv. Embryos treated on the 15th incubation day: The growth inhibition rates on the 18th in the groups treated with $50{\mu}C$ were 6.5% and in those treated with $150{\mu}C$ were 10.1% and in those treated with $250{\mu}C$ were 8.5% respectively. In summary, the longer the incubation days, the less the growth inhibition rates. II) The histopathological changes in the various organs were as follows: i. Bone: Hyperplasia and edematous changes of the bone cavity, irregular distribution of immature granular cells and increased number of the myeloblast, megakaryocyte and reticuloendothelial cells were noted. ii. Liver: The embryos treated with $150{\mu}C\;of\;^{35}S$ on the 8th incubation day showed necrosis and nucleolysis of the liver cell and abnormal enlargement of sinusoid on the 12th incubation day. The longer the incubation days, the more severe the changes such as the pyknotic artophy of the liver cells and heterochromatism. The embryos treated on the 5th incubation day with 50 and $150{\mu}C\;of\;^{35}S$ showed little changes, but sight enlargement and accumulation of serous fluid in the sinusoid on the 8th incubation day. iii. Kidney: No particular changes except atrophic changes of epithelium were noted in early stage, however, the infiltration of the granular cell and monocyte into the cortex and pyknotic changes of vascular glomeruli were noted in later stage. These changes were not closely related to the doses of $^{35}S$ given. iv. Gonad: The degenerative changes such as destruction of the immature germ cells, hyperplasia and vacuolization of the stroma were noted in testis and ovary. v. Eye: A slight distortion of the cornea and sclera was noted. The hypertrophy of inner layer and blood cell infiltration into the vascular layer of the choroid membrane were noted in embryo groups on the 12, 15 and 18th incubation days.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.22 no.3
• /
• pp.280-285
• /
• 1993

The bioactivity of garlic extract was evaluated, based on the inhibition of soybean lipoxygenase. While the inhibition of lipoxygenase by the chloroform extract (1$_{50}$ value after 10min precirculation, 55mg/$m\ell$) of garlic homogenate shows the property as irreversible inhibitors, the aqueous extract (1$_{50}$ value, 65mg/$m\ell$) appeared to contain mainly reversible inhibitors. In the related study, diallyldislufide and dimethyldisulfide inhibited the enzyme with 1$_{50}$ value of 1.3mM and 18mM, respectively. These disulfides demonstrated both irreversible and reversible patterns of inhibition. In addition, synthetic alliin(allylcysteine sulfoxide) was found to inhibit the enzyme at high concentration (approximately 22%, at 10mM), and its decomposition products showed the irreversible property in the inhibition, in contrast to S-ethyl cysteine sulfoxide which expressed no significant inhibition. Thus, it is suggested that the garlic macerate contains both irreversible and reversible sulfur inhibitors.itors.