• Journal of Microbiology and Biotechnology
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• v.4 no.3
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• pp.165-170
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• 1994

An efficient and convenient method of introducing transposable elements into acetic acid bacteria was developed by the method of conjugal transfer. The ampicillin-resistant strain, Acetobacter sp. HA, was selected to be conjugated with two E. coli strains, WA803 containing pGS9 and AC8001 harboring pJB4JI. The Tn5 containing suicide vector pGS9 or pJB4JI, was transferred from E. coli to Acetobacter sp. HA and kanamycin-ampicillin-resistant transconjugants obtained at high frequencies. The conjugal frequencies of pGS9 and pJB4JI were 6.20$\times$$l0^{-1} and 2.79$\times$l0{-1}$ per recipient, respectively. The transfer method was applied on four different strains of Acetobacter. The conjugal transfer frequencies ranged from 2.00$\times$$l0^{-2} to 4.45$\times$l0^{-8}$ per recipient in the three strains. Some transconjugants tested were found to contain Tn5 DNA in their genomes and this was confirmed by Southem blot analysis. This is the first study which shows that Tn5 mutagenesis can be applied to successfully isolate mutants of Acetobacter genus.

• Journal of Convergence for Information Technology
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• v.11 no.3
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• pp.87-94
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• 2021

This study was conducted to identify the mental health and quality of life of cardiovascular disease patients by their activities restriction through the second analysis of the 7th KNHANES collected from 2016 to 2018. The subjects of this study were 521 adults with angina or myocardial infarction who had no missing variables among 24,269 subjects. The difference between mental health and quality of life according to the activity restriction was analyzed by Chi-square method and the effect of mental health and quality of life by activity restriction by Logistic regression method using the SAS 9.4 version. The results of the analysis showed that the stress level, the depression for the last two weeks and suicide thought level was high when there was activity restriction, which had a significant effect on mental health. Mobility, selfcare and usual activities were disrupted, and pain/inconvenience and anxiety/depression increased when there was activity restriction, which also showed a significant effect on quality of life. Therefore, it is suggested that the development and operation of effective physical activity promotion program to minimize activity restriction is necessary to improve mental health and quality of life of cardiovascular patients.

• Convergence Security Journal
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• v.12 no.5
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• pp.51-59
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• 2012

Purpose of this study analyzes deeply and examine closely effect that incident shock of private guards gets in job stress. Used participation observation and In-depth analytical method to 10 common people guards inhabiting in capital region in 2012 to achieve purpose of this study. Collected interview data encoded using computer and analyzed by incident shock, job effect, job stress, organizational life. Conclusion proved in this study is as following. First, private guards appeared that incident shock such as suicide, terror threat, irrationality of superior that is produced in business spot gives great psychological fear. Second, private guards appeared suffer important shock are influenced in fear about business from shock that suffered in the past, and sociophobia appeared by thing which exert important effect changing by other business territory. Third, most private guards appeared that oneself experienced job stress from experiencing incident shock in the past. That is, incident shock exerts direct influence on customer confrontation in the past. Fourth, private guards appeared by thing which individual's organization life reduces when do not overcome incident shock that occur in the past. That is, incident shock decreases trust development and teamwork between superior and subordinate in organizational culture of private security.

• Korean Journal of Health Education and Promotion
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• v.24 no.5
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• pp.39-54
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• 2007

This study attempts to provide fundamental information for a health policy and health services by looking at the trends and types of the mortality rates in Korea and Japan. In this study, data of the death statistics of Korea and Japan over the 21-year period from 1983 to 2003 are analyzed. Mortality data are standardized ${\times}100,000$ to the 2003 Japan population, according to the direct method. In 2001/2003, the ranking of major causes of death in Korea and Japan has been greatly changed. In Korea, mortality rates from malignant neoplasms, diabetes mellitus, renal failure, falls and suicide have increased. In Japan, the mortality rates from pneumonia increase more than those from diabetes mellitus. In 2001/2003, the proportions of the mortality rates from chronic diseases are higher than those from acute diseases. In the Korean health promotion policy "Health Promotion 2020", a more intensive goal management is needed.

• Journal of Korean Public Health Nursing
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• v.27 no.1
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• pp.113-128
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• 2013

Purpose: The purpose of this study was to investigate the degree of suicidal risk, meaning in life, and life respect in various ages of patients and identify factors influencing their life respect. Method: The participants were 229 patients in a public hospital who completed questionnaires. Data were analyzed using descriptive statistics, t-test, Fisher's exact test, ANOVA with Duncan post hoc test, and multiple regression. Results: There was a negative correlation between the meaning of life and life respect in the old age group (r=-.23, p=.02) and all subjects (r=-.14, p=.01) after controlling for age. Factors significantly influencing life respect were gender (${\beta}$=0.11, p=.050) and educational status (${\beta}$=-0.17, p=.022), and the multiple regression model explained 16.7% of the variance in all subjects (p<.001). In the early adulthood group, factors significantly influencing the life respect were gender (${\beta}$=0.18, p<.001) and suicidal thoughts (${\beta}$=0.21, p=.028), and the multiple regression model explained 6.8% of variance in all subjects (p=.001). Conclusion: The results of this study suggest that suicidal prevention and educational programs for increasing an appreciation of life should consider subject's characteristics, such as gender and educational status.

• Journal of Korean Academy of Fundamentals of Nursing
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• v.20 no.4
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• pp.419-428
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• 2013

Purpose: This study was done to explore the level of life stress, depression, and suicidal ideation and to identify the associated factors of suicidal ideation (SI) among college nursing students. Method: The design of this study was a descriptive survey design using a convenience sampling. Data collection was done using self-questionnaire from 124 students in 2 universities located in Gyeonggi and Chungnam provinces. The collected data were analyzed using descriptive statistics, ANOVA, t-test, Pearson correlation coefficients, and hierarchical multiple regression. Results: Study participants reported low level of SI, depression, and life stress ($44.45{\pm}19.21$, $8.40{\pm}5.94$, $40.65{\pm}32.17$). The 53% of variance in SI was significantly explained by life stress related value (${\beta}=.41$, p<.001), life stress related to interpersonal relationship with family (${\beta}=.19$, p=.014), life stress related to interpersonal relationship with friends (${\beta}=.34$, p<.001), and depression (${\beta}=.36$, p<.001). Conclusions: The results indicate that to decrease the SI in these students, an active interest in the students is needed to lower stress levels in interpersonal relationships with family and friends, in life stress related to value, and for relief of depression. Suicide prevention programs specific to the level of life stress and depression need to be developed for nursing students.

• Journal of Home Health Care Nursing
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• v.21 no.2
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• pp.139-146
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• 2014

Purpose: This study was conducted to investigate the correlation between health status, health-promoting behavior, self-esteem and suicidal ideation among the elderly. Method: Subjects consisted of 115 elderly persons. Data were collected from January to March 2013, and were analyzed using descriptive statistics, a t-test, ANOVA, Pearson's correlation analysis. Results: The mean scores for health status ($2.41{\pm}.68$), health-promoting behavior ($2.38{\pm}.43$), and self-esteem ($2.87{\pm}.69$) among the elderly fell in the average range, whereas scores for suicidal ideation ($.48{\pm}.36$) among the elderly fell in the below average range. Health status differed significantly according to age, educational level and economic status. Health-promoting behavior did not differ significantly based on these general characteristics. Self-esteem differed significantly according to age, job, and number of people living in the household. Suicidal ideation differed significantly based on age, educational level, and economic status. Significant correlations were found between health status, health-promoting behavior, self-esteem, and suicidal ideation. Conclusion: These findings indicate that perceived health status, health-promoting behavior, and self-esteem may be factors that need to be considered in order to decrease the prevalence of suicidal ideation among the elderly. These results can be used to develop a suicide prevention program for the elderly.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• v.3 no.1
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• pp.129-137
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• 1992

This article describes several clinical usefullness of the telephone in psychotherapy with adolescents through case analysis by Tolchin, and discussed various management problems and some practical guidelines for therapists planning to utilize telephone psychotherapy with adolescents through literature analysis. In general, most adolescent patients in regular treatment benefit from knowing that the therapist can be reached by phone and that their call will be returned that same day. The therapist's telephone availability helps the young patient to develope a positive working alliance. Telephone psychotherapy had it's first widespread application in crisis intervention and suicide prevention. It's current uses also include enhancement of self-control in cases of self-destructive adolescents, and management of anxious, depressive, or anorexic adolescents. In addition, such widely divergent modalities of treatment as follow up of patients discharged from a psychiatric hospital, alcoholic rehabilitation programs, drug hotlines, sex counselling group therapy for visually impaired elderly, control of separation anxiety in girls, resolution of fear on the mental disorders, and helpful modality to continue psychotherapy without geographic barrier during longer periods of separation with therapist are currently available via telephone. But telephone psychotherapy is generally not suitable for more self-defensive and introverted cases, more dependent chronic callers, silent cases, test callers. the cases of telephone scatologias, the cases of intellectualized discussion, of more dependent cases calling phone in order to get attention from therapist. Telephone psychotherapy for adolescents serves as an important and useful method in the therapeutic armamentarium for this most challenging and rewarding population.

• Korean Journal of Clinical Pharmacy
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• v.23 no.1
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• pp.57-64
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• 2013

Purpose: Drug can be hazardous for people if misused although they are useful for their indication. In urgent incidences such as overdose, proper treatment for intoxication can save patients' lives. In emergent case regarding drug overdose, pharmacists should know how to provide correct information including antidote to other healthcare providers. However, in Korea, there is neither regular class nor education material regarding detoxification. Therefore, the object of our study is to investigate the perception of pharmacists about it. Method: We surveyed hospital pharmacists by means of self-reporting questionnaire in order to investigate the pharmacists' perception of detoxification treatments from May $12^{th}$ 2012 to August $10^{th}$ 2012. The questionnaire comprised of demographic information, interest in detoxification treatment (inquiry from patients and education about antidote), knowledge of antidote, effective drug search route and perception of the need for education and stocking materials about antidotes). Results: It included total 281 hospital pharmacists from 30 hospitals in S. Korea. Of them, only 16.7% have been questioned about drug overdose from patient or representative and 35% have learned about antidotes in case of drug overdose through education program of Korean association of hospital pharmacist or university. About 98% thought that education and stocking materials about frequently overdosed drug and antidotes are helpful for patients in emergent case. Also, the percentage of correct answer of each questions about antidotes were higher in educated group ($p{\leq}0.001$). The more work years are, the percentage of correct answer of each questions are higher ($p{\leq}0.001$). Conclusion: In conclusion, it will be helpful for reducing damage by drug overdose that pharmacists take regular education about antidote for all pharmacist and pharmacy student. In addition, preparing and keeping booklet for Korean Style-antidote in pharmacy is needed currently for protecting public health.

• The Journal of Information Systems
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• v.30 no.2
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• pp.29-56
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• 2021

Purpose Cyber deviance of adolescents has become a serious social problem. With a widespread use of smartphones, incidents of cyber deviance have increased in Korea and both quantitative and qualitative damages such as suicide and depression are increasing. Research has been conducted to understand diverse factors that explain adolescents' delinquency in cyber space. However, most previous studies have focused on a single theory or perspective. Therefore, this study aims to comprehensively analyze motivations of juvenile cyber deviance and to develop a predictive model for delinquent adolescents by integrating four different theories on cyber deviance. Design/methodology/approach By using data from Korean Children & Youth Panel Survey 2010, this study extracts 27 potential factors for cyber deivance based on four background theories including general strain, social learning, social bonding, and routine activity theories. Then this study employs econometric analysis to empirically assess the impact of potential factors and utilizes a machine learning approach to predict the likelihood of cyber deviance by adolescents. Findings This study found that general strain factors as well as social learning factors have positive effects on cyber deviance. Routine activity-related factors such as real-life delinquent behaviors and online activities also positively influence the likelihood of cyber diviance. On the other hand, social bonding factors such as community commitment and attachment to community lessen the likelihood of cyber deviance while social factors related to school activities are found to have positive impacts on cyber deviance. This study also found a predictive model using a deep learning algorithm indicates the highest prediction performance. This study contributes to the prevention of cyber deviance of teenagers in practice by understanding motivations for adolescents' delinquency and predicting potential cyber deviants.