• Korean Chemical Engineering Research
• /
• v.43 no.3
• /
• pp.380-386
• /
• 2005

Separation of fructose and glucose was performed using emulsion liquid membranes with a mixture of an organoboronic acid and a quaternary ammonium salt as a carrier in a batch reactor. In order to find a carrier and an optimal experimental condition suitable to the sugar separation, extraction of each sugar was carried out independently. The effect of various experimental variables, such as initial concentration of sugar in the feed phase, type of organoboronic acids, and w/o ratio, on the sugar separation was investigated, and the concentrations of sugars in each aqueous phase were analyzed. The ratio of degree of extraction of fructose to that of glucose was very high, but the concentration of fructose in the receiving phase was not too high. Therefore, a stronger stripping agent in the receiving phase was required for development of a practical ELM system suitable to the sugar separation.

• 한국생물공학회:학술대회논문집
• /
• 2002.04a
• /
• pp.504-507
• /
• 2002

A separation/purification process for enzymatic sugar ester production was investigated The crude reaction mixture contained sugar ester and unreacted fatty acid in acetone. The reaction mixture was mixed with KOH/phosphate buffer. Hexane was then added to enhance phase separation. Three phases formed: a lower aqueous phase containing nothing of interest, a polar organic solvent middle phase that contained mostly fatty acid soaps, and a hexane-rich upper phase that contained mostly sugar ester. Distribution coefficient of each component was measured.

• Korean Journal of Veterinary Service
• /
• v.23 no.2
• /
• pp.137-142
• /
• 2000

In order to develop a good separation and simultaneous analysis of different sugar in an artificial mixed sugar solution, we analyzed 10 sugar components in an artificial mixed sugar solution composed of fructose, glucose, mannitol, sucrose, maltose, lactose, xylose, xylitol erythritol, and trehalose with using HPLC-ELSD or HPLC-RI. Separation and quantification by HPLC-ELSD was superior to those by HPLC-RI and detection sensitivity by HPLC-ELSD was higher then that by HPLC-RI as micorgram($\mu\textrm{g}$) level. 1. The units of minimal detectable limits were showed $\mu\textrm{g}$/$m\ell$ and ng/$m\ell$ by the HPLC-RI and HPLC-ELSD, respectively. 2. The condition of ELSD was drift tube temperature $82^{\circ}C$, $N_2$ gas flow rate 2.10 SLPM, and colum oven temperature $30^{\circ}C$, respectively. Isolation and recovery rates of single sugar from the multiple sugar solution was higher at the condition (time: flow rate: D.W.:ACN MeOH, min : $m\ell$/min:v:v:v) of linear gradient elution of mobile phase as 0 : 1.00 : 15 : 85 : 0.1 : 1.00 : 6 : 90 : 4, 17 : 1.00 : 10 : 70 : 20, 28 : 1.00 : 15 : 85 : 0 an 35 : 1.00 : 15 : 85 : 0, in order.

• KSBB Journal
• /
• v.4 no.3
• /
• pp.281-287
• /
• 1989

The experimental study reported herein was to investigate to separation characteristics of a tangential flow membrane in a continuous recycle situation. Physical and dynamic analyses are performed on the membrane system in order to relate relevant variables to the capacity of separation. The results of separation process may be summarized by a proposed equation:Sh=A(Re.Sc.dh/L)1/3. It was shown also by the analyses that effective separation of sugar and cell was attainable by means of tangential membranes, thereby enhancing ethanol productivity in fermentation with continuous cell and substrate recycle.

• Food Engineering Progress
• /
• v.14 no.4
• /
• pp.346-353
• /
• 2010

Due to the globalization of Korean foods, there are great interests in traditional Korean foods. Thus, the enhancement and development of makgeolli processing have been constantly accomplished. In case of makgeolli, the storage stability is very important because the fermentation of makgeolli during distribution is still progressed. Therefore, the objective of this study was to investigate storage stability of makgeolli by separation storage methods. During the 30-day storage at $10^{\circ}C$, pH value, titratable acidity, color value, sugar content, reducing sugar content, and alcohol content were measured. Microbial cell counts were also evaluated. Reducing sugar content was decreased after 10 days for all the samples. In the case of titratable acidity and color, these values were constantly increased with storage time. Especially, the yellowness value of the precipitate of makgeolli was increased by two times than that of the beginning. There was a decreasing tendency for lactic acid bacteria with storage time. In case of yeast, there was a decreasing tendency after 15 days, but the significance was not detected. The quality changes in the samples from centrifugal separation were relatively less than the control. Therefore, the separation storage method could affect the enhancement of makgeolli quality during distribution.

• Proceedings of the PSK Conference
• /
• 2002.10a
• /
• pp.398.1-398.1
• /
• 2002

A new chiral derivatization agent with sugar moiety. 2, 3.4, 6-tetra-O-acetyl-D-galactopyranosyl isothiocyanate (GATC) was synthesized. Several $\beta$-blockers were investigated for the possible separation of the enantiomers by reversed-phase HPLC after derivatization with this new chiral derivatization agent (GATC). GATC was reacted readily with $\beta$-blockers at room temperature and the reaction mixture could directly be injected into the HPLC system. (omitted)

• KSBB Journal
• /
• v.8 no.2
• /
• pp.178-184
• /
• 1993

Extractive fermentations with the extract of Jerusalem artichoke in an aqueous-two-phase-system of polyethyleneglycol(PEG) and dextran(Dx) were investigated to obtain the effects of composition of PEG and Dx on both fermentation ,characteristics and partition ratio of alcohol. The specific growth rate of K. Fraglis CBS 1555 increased with a decrease of concentration of PEG and Dx. It augmented along with concentration of initial sugar up to 80g/l but decreased thereafter. The specific production rate of alcohol showed a rising tendency up to 100g/lof initial sugar, whereafter represented a decreasing trend. The partition ratio of alcohol between two phases augmented according to decrease of Dx comic. and increase of PEG cone. regardless of initial sugar concentrations. The ratio, however, decreased with Increment of initial sugar concentration at constant composition of PEG and Dx. The partition coefficient of alcohol had an ascending effect to the increase of PEG cone, but it had little effect on the changes of concentrations of Dx and initial sugar. The present study suggests that the optimum composition of PEG and Dx in the aqueous-two-phase-system by extractive fermentation were around 6.5%(w/v) of PEG and 3%(w/v) of Dx in considerations of emulsion state, sedimentation and separation of two phases, alcohol partition ratio, and specific growth rate.

• 한국생물공학회:학술대회논문집
• /
• 2000.11a
• /
• pp.630-631
• /
• 2000

Sugar esters are produced by transesterification of fatty acids and sugars in organic solvent media. Chromatographic separation of sugar esters and fatty acids was investigated. $C_{18}$ column($3.9{\times}300mm$) was used in the chromatography experiment. The effect of flow rate, column temperature, and sample loading on the charateristics of chromatogram was studied. Sugar esters and fatty acids tested in this study was successfully separated with a high resolution.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
• /
• 2001.06a
• /
• pp.1264-1264
• /
• 2001

The affect of surface temperatures of fruits on spectrum which measures actual sugar contents was observed. PLSR was applied to develop the sugar content evaluation system that was not affected by temperature. The reflected spectrum was used at the wavelengths of 654 and 1052nm with the separation distance of 2.5nm. To increase the conformance of a model using unknown samples, let the minimum value of PRESS be an optimum factor. 71 Shingo pears stored in a refrigerator were left in a room temperature for a while and these temperatures and reflected spectrums were measured. Reflected spectrums were measured at the wavelengths of 654 and 1052nm, 3 samples in one second. To measure these at different temperatures, the experiment was repeated hourly and four times. Starting temperatures of 2-3 were increased up to 17. The total number of measured spectrum was 284. To develop a sugar content evaluation system model using measured reflected spectrum, three groups of samples were considered. First group had 51 samples at 14 and second group had 141 samples with lower or higher temperatures than 14. Third group had 155 samples with well distributed temperatures. Other samples were used as validations to ensure the conformance. Measuring the sugar contents of samples with surface temperatures other than 14 were difficult with PLS model I, developed by using a sample temperature of 14. If the sugar contents were compensated using samples' temperatures, results of prediction would be close to the expected results and it would be one of the most important factors to develop this system. PLS models I and II could compensate the temperature but the precision would not come up to the standard. High precision was expected by using samples with wide ranges of temperatures and sugar contents. Both models showed the possibility of an improvement of a sugar content evaluation system disregarding the temperature. For practical use of a system, selecting samples should be done carefully to reduce the effect of the temperature.

• Korean Journal of Animal Reproduction
• /
• v.3 no.1
• /
• pp.56-60
• /
• 1979

This study was carried out to find the difference between the naturaly born sex rtio among 1,242 head of pigs(120 litters) at Swine Farm, Cheil Sugar Co. and B-body a, pp.arance from their semen, and to find the conception rates which were inseminated to 40 sows with sperm from the anode and cathode after electrophoresis of boar semen. In order to the electrophoretic separation, the semen was placed into the platimum loop electrodes(105 cc) at room temperature for 30 minutes with D.C. 3V. and 350${\mu}$A. constant. The sperm fluorescent staining method was performed in accordance with Bhattacharya's(1970) method. The spermatozoa were observed through a Olympus Vanox microscope(made in Japan) using exciter filter with I heat barrier HPO 120. The results obtained were summarized as follows: 1. The natural sex ratio of 1,242 piglets(120 litters) which were born at Swine Farm, Cheil Sugar Co. was 50%, and B-body a, pp.arance of its boar semen were 49.24%. 2. With electrophoretic separation, the anode and cathode attracted 65.5${\pm}$5.03% and 29.89${\pm}$4.29% of B-body bearing sperm, respectively. 3. After electrophoresis of boar sperm, they were inseminated to 40 sows with sperm from anode and cathode. The conception rate was 92.5%.