• 제목/요약/키워드: Succinate

검색결과 397건 처리시간 0.025초

Characterization of Yellow Mutants Isolated from the Red Yeast Phaffia rhodozyma (Xanthophyllomyces dendrorhous)

  • An, Gil-Hwan
    • Journal of Microbiology and Biotechnology
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    • 제6권2호
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    • pp.110-115
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    • 1996
  • Yellow mutants of the astaxanthin producing red yeast Phaffia rhodozyma were obtained by nitrosoguanidine mutagenesis. The carotenoid composition of the yelow mutants, Yan-1 and Ny-1, was mainly $\beta$ -carotene (> 95$%$) and torulene (< 5$$). Therefore, the yellow mutants are carotene oxygenation deficient mutants (CODMs). CODMs produced decreased quantities of carotenoids compared to their red parents and this indicated that carotene may regulate its synthesis. CODMs, Yan-1 and Ny-4, on plates containing 50 $\mu$ M antimycin, showed highly pigmented vertical papillae. Antimycin-induced mutants purified from the papillae showed increases in carotenoid content (up to 1 mg $\beta$-carotene/g yeast). CODMs, Yan-1 and Ay-1, were more sensitive to antimycin than red strains, Ant-1 and 67-385. This was probably due to lower antioxidant activity of $\beta$-carotene than that of astaxanthin. Light increased torulene and light+antimycin further increased the torulene. Yan-1 and Ny-4 could grow with succinate, though their red parents, Ant-1 and Anf-1p, could not. However, antimycin induced mutation of Yan-1 or Ny-4 destroyed the ability to grow with succinate.

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Poly(butylene succinate) ionomer의 결정화 거동과 분해 (Crystallization behaviour and Degradation of Poly(butylene succinate) ionomer)

  • Han, Sang-Il;Kim, Dong-Kuk;Im, Seung-Soon
    • 한국섬유공학회:학술대회논문집
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    • 한국섬유공학회 2003년도 봄 학술발표회 논문집
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    • pp.79-82
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    • 2003
  • An ionomer is defined as an ion-containing polymer with a small amount (usually up to 10-15 mol%) of ionic groups along the backbone chains or as pendant groups. Ionomers have been extensively studied because of the significant changes in their physical properties due to the formation of ionic aggregates, such as enhanced mechanical properties, high melt viscosity, and increased thermal properties.$\^$1-5/ Although there have been many studies of ionomers, the crystallization behaviour of degradable ionomers is still not understood. (omitted)

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Responses of Chloramphenicol Immunosensor to Analyte Types

  • PARK , IN-SEON;KIM, DONG-KYUNG;KIM, NAM-SOO
    • Journal of Microbiology and Biotechnology
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    • 제14권6호
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    • pp.1157-1162
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    • 2004
  • A well-holder type piezoelectric chloramphenicol (CAP) immunosensor which was prepared by binding an anti­CAP antibody to the chemisorbed monolayers of various thiol or sulfide compounds over the gold electrode surface of quartz crystals through a carboxyl-amine coupling procedure, using the activation with l-ethyl- 3-(3-dimethylarninopropyl)carbodiimide­HCl and N-hydroxysulfosuccinimide, was determined for its responses to CAP, CAP succinate, and water-soluble CAP. The reaction phase used in the well holder was 0.01 M phosphate buffer (pH 7.4), and the solvent for analyte dissolution varied according to the solubility of the individual analyte. The analyte detection which was indicated by a steady-state frequency shift was finished within 10 min, except for CAP dissolved in methanol. The responses of CAP succinate and water-soluble CAP in the reaction phase were very stable, while a minute fluctuation was found with CAP.

Isolation of a Marine Bacterium Capable of Biodegrading Poly(butylene succinate)

  • Lee, Sang Jun;Park, Eun Hee;Han, Yun Hee;Kim, Young Ok;Park, Seong Wook
    • Fisheries and Aquatic Sciences
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    • 제16권1호
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    • pp.41-44
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    • 2013
  • We developed a poly(butylene succinate) (PBS) indicator plate and isolated a marine bacterial colony capable of biodegrading PBS based on the appearance of a clear zone. Growth of the PBS-2 isolate was observed over 4 days of culture at $37^{\circ}C$ in PBS-tryptone basal liquid medium, but not in PBS-deprived control medium. The PBS-2 isolate was named Paenibacillus sp. PBS-2 based on 16S rDNA gene sequencing. The PBS-biodegrading marine bacterium isolated in this study will contribute to the effective management of PBS waste problems in marine environments.