Browse > Article
http://dx.doi.org/10.5657/FAS.2013.0041

Isolation of a Marine Bacterium Capable of Biodegrading Poly(butylene succinate)  

Lee, Sang Jun (Biotechnology Research Division, National Fisheries Research & Development Institute)
Park, Eun Hee (Biotechnology Research Division, National Fisheries Research & Development Institute)
Han, Yun Hee (Biotechnology Research Division, National Fisheries Research & Development Institute)
Kim, Young Ok (Biotechnology Research Division, National Fisheries Research & Development Institute)
Park, Seong Wook (Fisheries System Engineering Division, National Fisheries Research & Development Institute)
Publication Information
Fisheries and Aquatic Sciences / v.16, no.1, 2013 , pp. 41-44 More about this Journal
Abstract
We developed a poly(butylene succinate) (PBS) indicator plate and isolated a marine bacterial colony capable of biodegrading PBS based on the appearance of a clear zone. Growth of the PBS-2 isolate was observed over 4 days of culture at $37^{\circ}C$ in PBS-tryptone basal liquid medium, but not in PBS-deprived control medium. The PBS-2 isolate was named Paenibacillus sp. PBS-2 based on 16S rDNA gene sequencing. The PBS-biodegrading marine bacterium isolated in this study will contribute to the effective management of PBS waste problems in marine environments.
Keywords
PBS indicator plate; Growth kinetics; 16S rDNA gene; PBS waste problems;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Yoon JH, Lee ST and Park YH. 1998. Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48, 187-194. http://dx.doi.org/10.1099/00207713-48-1-187.   DOI   ScienceOn
2 Zhao JH, Wang XQ, Zeng J, Yang G, Shi FH and Yan Q. 2005. Biodegradation of poly(butylene succinate) in compost. J Appl Polym Sci 97, 2273-2278. http://dx.doi.org/10.1002/app.22009.   DOI   ScienceOn
3 Chun J, Lee JH, Jung Y, Kim M, Kim S, Kim BK and Lim YW. 2007. EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259-2261. http://dx.doi.org/10.1099/ijs.0.64915-0.   DOI   ScienceOn
4 Fujimaki T. 1998. Processability and properties of aliphatic polyesters, 'Bionolle', synthesized by polycondensation reaction. Polym Degrad Stab 59, 209-214. http://dx.doi.org/10.1016/S0141-3910(97)00220-6.   DOI   ScienceOn
5 Hayase N, Yano H, Kudoh E, Tsutsumi C, Ushio K, Miyahara Y, Tanaka S and Nakagawa K. 2004. Isolation and characterization of poly(butylene succinate-co-butylene adipate)-degrading microorganism. J Biosci Bioeng 97, 131-133. http://dx.doi.org/10.1263/jbb.97.131.   DOI
6 Kleeberg I, Hetz C, Kroppenstedt RM, Müller RJ and Deckwer WD. 1998. Biodegradation of aliphatic-aromatic copolyesters by Thermomonospora fusca and other thermophilic compost isolates. Appl Environ Microbiol 64, 1731-1735.
7 Maeda H, Yamagata Y, Abe K, Hasegawa F, Machida M, Ishioka R, Gomi K and Nakajima T. 2005. Purification and characterization of a biodegradable plastic-degrading enzyme from Aspergillus oryzae. Appl Microbiol Biotechnol 67, 778-788. http://dx.doi.org/10.1007/s00253-004-1853-6.   DOI
8 Masaki K, Kamini NR, Ikeda H and Iefugi H. 2005. Cutinase-like enzyme from the yeast Cryptococcus sp. strain S-2 hydrolyzes polylactic acid and other biodegradable plastics. Appl Environ Microbiol 71, 7548-7550. http://dx.doi.org/10.1128/AEM.71.11.7548-7550.2005.   DOI   ScienceOn
9 Mohanty AK, Misra M and Hinrichsen G. 2000. Biofibers, biodegradable polymers and biocomposites: an overview. Macromol Mater Eng 276/277, 1-24. http://dx.doi.org/10.1002/(SICI)1439-2054(20000301)276:1<1::AID-MAME1>3.0.CO;2-W.   DOI   ScienceOn
10 Sakai Y, Isokawa M, Masuda T, Yoshioka H, Hayatsu M and Hayano K. 2002. Usefulness of soil p-nitrophenyl acetate esterase activity as a tool to monitor biodegradation of polybutylene succinate (PBS) in cultivated soil. Polym J 34, 767-774. http://dx.doi.org/10.1295/polymj.34.767.   DOI   ScienceOn
11 Tansengco ML and Tokiwa Y. 1998. Thermophilic microbial degradation of polyethylene succinate. World J Microbiol Biotechnol 14, 133-138. http://dx.doi.org/10.1023/A:1008897121993.   DOI
12 Tokiwa Y and Jarerat A. 2003. Microbial degradation of aliphatic polyesters. Macromol Symp 201, 283-289. http://dx.doi.org/10.1002/masy.200351131.   DOI   ScienceOn
13 Uchida H, Nakajima-Kambe T, Shigeno-Akutsu Y, Nomura N, Tokiwa Y and Nakahara T. 2000. Properties of a bacterium which degrades solid poly(tetramethylene succinate)-co-adipate, a biodegradable plastic. FEMS Microbial Lett 189, 25-29. http://dx.doi.org/10.1016/S0378-1097(00)00246-9.   DOI   ScienceOn