• Journal of Microbiology and Biotechnology
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• v.23 no.6
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• pp.779-784
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• 2013

Leptospirosis is a worldwide zoonosis of global concern caused by Leptospira interrogans. The availability of ligand libraries has facilitated the search for novel drug targets using chemogenomics approaches, compared with the traditional method of drug discovery, which is time consuming and yields few leads with little intracellular information for guiding target selection. Recent subtractive genomics studies have revealed the putative drug targets in peptidoglycan biosynthesis pathways in Leptospira interrogans. Aligand library for the murD ligase enzyme in the peptidoglycan pathway has also been identified. Our approach in this research involves screening of the pre-existing ligand library of murD with related protein family members in the putative drug target assembly in the peptidoglycan biosynthesis pathway. A chemogenomics approach has been implemented here, which involves screening of known ligands of a protein family having analogous domain architecture for identification of leads for existing druggable protein family members. By means of this approach, one murC and one murF inhibitor were identified, providing a platform for developing an anti-leptospirosis drug targeting the peptidoglycan biosynthesis pathway. Given that the peptidoglycan biosynthesis pathway is exclusive to bacteria, the in silico identified mur ligase inhibitors are expected to be broad-spectrum Gram-negative inhibitors if synthesized and tested in in vitro and in vivo assays.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.15 no.2
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• pp.38-45
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• 2016

Since a 5-axis machine tool has two rotary axes, it offers numerous advantages, such as flexible accessibility, longer tool life, better surface finish, and more accuracy. Moreover, it can conduct whole machining by rotating the rotary feed axes while setting the fixture at once without re-fixing in contrast to conventional 3-axis machining. However, it is difficult to produce complicated products that have a hollow shape. In contrast, 3D printing can produce an object with a complicated hollow shape easily and rapidly. However, because of layer thickness and shrinkage, its surface finish and dimensional accuracy are not adequate. Therefore, this study proposes hybrid technology by integrating the advantages of these two manufacturing processes. 3D printing was used as the additive manufacturing rapidly in the whole body, and 5-axis machining was used as the subtractive manufacturing accurately in the joining and driving places. The reliability of the proposed technology was verified through a comparison with conventional technology in the aspects of processing time, surface roughness. and dimensional accuracy.

• Journal of Electrical Engineering and Technology
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• v.9 no.6
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• pp.1909-1913
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• 2014

In this study, a superconducting fault current limiter (SFCL) having two magnetic paths was proposed, and its current limiting characteristics were analyzed. For the SFCL to effectively perform the current limiting operation, it must be designed considering the magnetic saturation of the E-I core. Further, the influence of the magnetic flux on its peak current limiting characteristics was investigated. In addition, the magnetic flux curves of the SFCL obtained from the fault current limiting experiments were analyzed, and the subtractive polarity winding case was observed to not only further reduce the saturation potential of the core but also perform the peak current limiting functions well when compared with the additive polarity winding case.

• Proceedings of the KIEE Conference
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• 2005.11b
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• pp.368-370
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• 2005

Superconducting fault current limiter (SFCL) provides the effect such as enhancement in power system reliability due to limiting fault current in a few miliseconds. The flux-lock type SFCL among various type SFCLs consists of two coils wound on the same iron core and a component using the YBCO thin film. In the SFCL, operation characteristics can be controlled by adjusting the inductances and the winding directions of the coils. In this paper, we investigated the various fault current limiting characteristics according to the voltage level. To analyze the current limiting performance, we compared operational characteristics on the subtractive polarity winding direction on in case of open-loop iron core.

• Journal of Information Display
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• v.6 no.3
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• pp.12-17
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• 2005

The need for low cost, flexible, thin film transistor (TFT) display backplanes has focused attention on new processing techniques and materials. We report the development of TFT backplane technology based entirely on jet-printing, using a combination of additive and subtractive processing, to print active materials or etch masks. The technique eliminates the use of photolithography and has the potential to reduce the array manufacturing cost. The printing technique is demonstrated with both amorphous silicon and polymer semiconductor TFT arrays, and we show results of small prototype displays.

• Journal of Mechanical Science and Technology
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• v.19 no.2
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• pp.558-566
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• 2005

We propose a feature-based multi-resolution representation of B-rep solid models using history-based Boolean operations based on the merge-and-select algorithm. Because union and subtraction are commutative in the history-based Boolean operations, the integrity of the models at various levels of detail (LOD) is guaranteed for the reordered features regardless of whether the features are subtractive or additive. The multi-resolution solid representation proposed in this paper includes a non-manifold topological merged-set model of all feature primitives as well as a feature-modeling tree reordered consistently with a given LOD criterion. As a result, a B-rep solid model for a given LOD can be provided quickly, because the boundary of the model is evaluated without any geometric calculation and extracted from the merged set by selecting the entities contributing to the LOD model shape.

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2002.10a
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• pp.167-167
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• 2002

Methylmercury (MeHg), one of the heavy metal compound, can cause severe damage to the central nervous system in humans. Many reports have contributed MeHg poisoning to contaminated foods and release into the environment. Despite many studies on the pathogenesis of MeHg-induced central neuropathy, no useful mechanism of toxicity has been established. To find genes differentially expressed by MeHg in neuronal cell, we peformed forward and reverse suppression subtractive hybridization (SSH) method on mRNA derived from neuroblastoma cell line, SH-SY5Y treated with solvent (DMSO) and 6.25 uM (IC$\sub$50/) MeHg. Differentially expressed CDNA clones were sequenced and the mRNAs were re-examined on Northern blots. These sequences were identified by BLAST homology search to known genes or expressed sequence tags (ESTs). Analysis of these sequences has provided an insight into the biological effects of MeHg in the pathogenesis of neurodegenerative disease and a possibility to develop more efficient and exact monitoring system of heavy metals as common environmental pollutants.

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2003.10a
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• pp.177-177
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• 2003

Methylmercury (MeHg) is a well-known neurotoxicant that causes severe damage to the central nervous system in humans. Many reports have shown that MeHg is poisonous to human body through contaminated foods and has released into the environment. Despite many studies on the pathogenesis of MeHg-induced central neuropathy, no useful mechanism of toxicity has been established so far. In this study, suppressive subtractive hybridization (SSH) was performed to identify differentially expressed genes on human neuroblastoma cell line, SH-SY5Y treated with DMSO and MeHg (6.25 uM) for 6 hr. Differentially expressed cDNA clones were sequenced and were screened by dot blot to eliminate false positive clones. 13 of 35 screened genes were confirmed using real time RT-PCR. These genes include EB1,90-kDa heat-shock protein, chromosome condensation-related SMC-associated protein and brain peptide Al, etc. Analysis of these genes may provide an insight into the neurotoxic effects of MeHg in human neuronal cells and a possibility to develop more efficient and exact monitoring system of heavy metals as ubiquitous environmental pollutants.

• Journal of Technologic Dentistry
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• v.41 no.2
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• pp.81-86
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• 2019

Purpose: The purpose of the present study was to compare the internal fit of two different temporary restorations fabricated by dental CAD/CAM system and to evaluate clinical effectiveness. Methods: Composite resin tooth of the maxillary first molar was prepared as occlusal reduction(2.0mm), axial reduction(1mm offset), vertical angle(6 degree) and chamfer margin for a temporary crown and duplicated epoxy die was fabricated. The epoxy dies were used to fabricate provisional restorations by CAD/CAM milling technique or 3D-printing technique. The inner data from all crowns were superimposed on the master die file in the 'best-fit alignment' method using 3D analysis software. Statistical analysis was performed using a Wilcoxon's rank sum test for differences between groups. Results: It showed that the internal RMS(Root Mean Square) values of the additive group were significantly larger than those of other group. No significant differences in internal discrepancies were observed in the temporary crowns among the 2 groups with different manufacturing method. Conclusion: All the groups had the internal fit within the clinical acceptable range (< $50{\mu}m$). The continuous research in the future to be applied clinically for the adaptation of additive manufacturing technique are needed.

• Proceedings of the Materials Research Society of Korea Conference
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• 2011.05a
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• pp.4.1-4.1
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• 2011

Recently, circuit printing technology has been considered as a promising alternative to conventional PCB fabrication, for it can greatly reduce the manufacturing costs. Even though printed circuit has many advantages over typical subtractive technology such as fewer processes, it has some disadvantages. The major problems are low adhesion and poor resolution. Efforts to overcome these problems have been mainly focused on ink developments with a limited success. And surface treatments showed some improvements. Therefore, various plasma treatments and primer coatings on plastic substrates have been tested. Plasma treatments using hydrocarbon gases including methane and propane improved the pattern quality of the inkjet printed circuit, which are further improved upon heating of substrate. On the other hand, there is little effect on the adhesion, which is improved only by a special primer coating. The adhesion of inkjet printed circuit has been increased more than 10 times upon treatment. As for the screen printed circuits, the overall effects are less significant since there is some organic binder in the ink. Nonetheless, the treatment has strong positive effects on pattern quality and adhesion. The adhesion of 1 kgf/cm2, which is comparable with those of the conventional PCB circuits, is possible through primer coating for both screen and inkjet printed circuits. The resulting circuit also showed good thermal, mechanical and electrical properties.