• IMMUNE NETWORK
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• v.1 no.3
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• pp.221-229
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• 2001

Background: Inactivation of tumor suppressor genes is believed to be important in the development of many human malignancies. Recently, several lines of evidence have indicated that the wild type p53 gene located at 17p13.3, may function as a tumor suppressor gene and that a mutant p53 gene could promote transformation by inactivating normal p53 function in a dominant negative fashion. These broad spectrum of p53 mutation in human cancers provide that mutant p53 and their protein may be potential targets of tumor diagnostic and therapeutic interventions. Method: Colony formation was performed to investigate growth suppressional ability. p53 expression pattern was examined by western blot and p53-mediated transactivation ability was assessed by CAT activity. SNU C2A cells were observed in apoptotic aspects induced by etoposide and $H_2O_2$ treatment, detecting sensitivity on agent, DNA fragmentation through agarose gel, chromatin condensation by fluorescence microscope, and cell cycle distribution by FACS. Result: 1) p53 mutant his179arg ($histidine{\rightarrow}arginine$) detected in SNU C2A cells lost transcriptional activity and growth suppression ability, showing dominant negative effect on its wild type p53. 2) Etoposide-treated SNU C2A cells induced apoptosis, exhibiting dramatic reduction of cell growth, DNA fragmentation, nuclear condensation formation of apoptotic body and increment of sub-G1 cell fraction. 3) Etoposide and $H_2O_2$-treated SNU C2A cells have no high increase of p53 expression and overexpressed p53 protein changed localization, from cytoplasm to nucleus. Also, p53-mediated transcriptional activity was increased by agents-treatment. Conclusion: SNU C2A cells coexpress wild-type and mutant p53 protein induced apoptosis in the condition on DNA damage, through localizational shift from cytoplasm to nucleus of p53 protein rather than the induction of p53 protein. SNU C2A cells derived mutant p53 his179arg abrogated both the growth supression ability and transactivational activity, showing inhibition effect on transcriptional activity of wild type p53, but did not repress the activity of wild type p53 in SNU C2A cells owing to dominant activity of wild type. These cell condition may provide new gene therapeutic implications leading effective antiproliferation of cell when mutant and wild-type p53 protein were co-expressed in cell.

• IMMUNE NETWORK
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• v.3 no.1
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• pp.53-60
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• 2003

Background: The role of macrophages in tumor angiogenesis is known to be the production of angiogenic cytokines and growth factors including TNF-${\alpha}$. Recently, macrophage also can produce the INF-${\gamma}$ that is being studied to be involved in angiogenic inhibition. Thus, the importance of macrophages in tumor angiogenesis is might being an angiogenic switch. Thus, the hypothesis tested here is that TNF-${\alpha}$ can modulate the INF-${\gamma}$ production in the macrophages from tumor environment as a part of tumor angiogenic switch. Methods: Macrophages in tumor environment were obtained from the peritoneal cavity of C57BL/6 mice injected with B16F10 melanoma cell line for 6 or 11 days. $Mac1^+$-macrophages were purified using magnetic bead ($MACs^{TM}$; Milteny Biotech, Germany) and cultured with various concentrations of TNF-${\alpha}$ for various time points at $37^{\circ}C$. The supernatants were analyzed for IFN-${\gamma}$ or VEGF by ELISA kit (Endogen, Woburn, MA). Results: Residential macrophages from the peritoneal cavity did not respond to LPS or TNF-${\alpha}$ to produce INF-${\gamma}$. However, the cells from tumor environment produced IFN-${\gamma}$ as well as VEGF and upregulated by the addition of LPS or TNF-${\alpha}$. RT-PCR analysis revealed the external TNF-${\alpha}$-induced IFN-${\gamma}$ gene expression in the macrophages from tumor environment. Conclusion: The overall data suggest that the macrophages in tumor environment might have an important role not only in angiogenic signal but also in anti-angiogenic signal by producing related cytokines. And TNF-${\alpha}$ might be a key cytokine in tumor angiogenic switch.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.26 no.3
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• pp.227-247
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• 2015

An X-band $8{\times}16$ dual-polarized active phased array antenna system has been implemented based on the substrate integrated waveguide(SIW) technology having low propagation loss, complete EM shielding, and high power handling characteristics. Compared with the microstrip case, 1 dB less is the measured insertion loss(0.65 dB) of the 16-way SIW power distribution network and doubled(3 dB improved) is the measured radiation efficiency(73 %) of the SIW sub-array($1{\times}16$) antenna element. These significant improvements of the power division loss and the radiation efficiency using the SIW, save more than 30 % of the total power consumption, in the active phased array antenna systems, through substantial reduction of the maximum output power(P1 dB) of the high power amplifiers. Using the X-band $8{\times}16$ dual-polarized active phased array antenna system fabricated by the SIW technology, the main radiation beam has been steered by 0, 5, 9, and 18 degrees in the accuracy of 2 degree maximum deviation by simply generating the theoretical control vectors. Performing thermal cycle and vacuum tests, we have found that the SIW array antenna system be eligible for the space environment qualification. We expect that the high efficiency SIW array antenna system be very effective for high performance radar systems, massive MIMO for 5G mobile systems, and various millimeter-wave systems(60 GHz WPAN, 77 GHz automotive radars, high speed digital transmission systems).

• The KIPS Transactions:PartC
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• v.15C no.6
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• pp.573-586
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• 2008

The improved performance and miniaturization of computer and the improvement of wireless communication technology have enabled the emergence of many high quality services. Among them multicast services are receiving much attention and their usage is increasing due to the increase of Internet multimedia services such as video conference, multimedia stream, internet TV, etc. Security plays an important role in mobile multicast services. In this paper, we proposed a secure multicast protocol for a hierarchical micro-mobility environment. The proposed secure multicast protocol provides security services such as authentication, access control, confidentiality and integrity using mechanisms including symmetric/asymmetric key crypto-algorithms and capabilities. To provide forward/backward secrecy and scalability, we used sub-group keys based on the hierarchical micro-mobility environment. With this security services, it is possible to guard against all kinds of security attacks performed by illegal mobile nodes. Attacks executed by internal nodes can be thwarted except those attacks which delete packet or cause network resources to be wasted. We used simulator to measure the performance of proposed protocol. As a result, the simulation showed that effect of these security mechanisms on the multicast protocol was not too high.

• The Journal of the Korea Contents Association
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• v.11 no.4
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• pp.1-10
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• 2011

While the broadband network and multimedia technologies have been developing, the commercial market of digital contents has also been widely spreading with recently starting IPTV. Generally, PC player can display digital contents obtained through middleware like a settop box and can only bring the informations about contents like CODEC, bitrate etc. useful for only experts. But general users want to know more optional informations like content's subject, description etc. So unlike previous PC player, we proposed a player system that can get inserted informations, namely EPG(Electronic Program Guide), without database after bringing contents to PC through settop box. In addition, we also proposed DCT(Discrete Cosine Transform) based blind watermark generating method to insert EPG informations. We can extract watermark without original image and insert robust watermark in proportion to coefficients in frequency domain. And we analyzed and parsed PSI data from MPEG-TS. So we could insert wanted information using watermark from EPG. And we composed UI by extracting EPG information from watermark interted contents. Finally we modularized whole system into the watermark insert/extract application and directshow filter based player. So we tried to design this system so that the general developer can do in a way that is easier and faster.

• Journal of the Korean Society for information Management
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• v.35 no.2
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• pp.89-114
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• 2018

Research on changes in research trends in academic disciplines is a method that enables observation of not only the detailed research subject and structure of the field but also the state of change in the flow of time. Therefore, in this study, in order to observe the changes of research trend in library and information science field in Korea, co-word analysis was conducted with Korean author keywords from three types of journals which were listed in the Korea Citation Index(KCI) and have top citation impact factor were selected. For the time series analysis, the 15-year research period was accumulated in 5-years units, and divided into 2003~2007, 2003~2012, and 2003~2017. The keywords which limited to the frequency of appearance 10 or more, respectively, were analyzed and visualized. As a result of the analysis, during the period from 2003 to 2007, the intellectual structure composed with 25 keywords and 8 areas was confirmed, and during the period from 2003 to 2012, the structure composed by 3 areas 17 sub-areas with 76 keywords was confirmed. Also, the intellectual structure during the period from 2003 to 2017 was crowded into 6 areas 32 consisting of a total of 132 keywords. As a result of comprehensive period analysis, in the field of library and information science in Korea, over the past 15 years, new keywords have been added for each period, and detailed topics have also been subdivided and gradually segmented and expanded.

• The Journal of the Korea Contents Association
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• v.9 no.8
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• pp.129-137
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• 2009

While the broadband network and multimedia technologies have been developing, the commercial market of digital contents also has been widely spreading with recently starting IPTV. As the IPTV services are getting to be generalized and popularized, the contents protection and authentication system tends to draw more attentions. So we need a system that can protect contents and allow only authenticated person to use right service by controling user authority and using content encryption. Until now, the conventional protection and authentication system is taking advantages of merits both in CAS and DRM. But the weak point of this system are in high costs, complexity and using HW. For resolving these problems, in this paper, we proposed IPTV contents protection and authentication system using OTP. When we transmit the content encrypted by OTP key using contents delivery technology, we operate XOR with contents using another settop-box's OTP key which was transmitted from distribution server. And contents are reconstructed and transmitted to the settop-box, In the end, downloaded content are encrypted by OTP key and are superior in content protection when contents redistribution. Since OTP use double-authentication elements in encryption process, this method is excellent in content protection. And it is very effective in cost aspect because it could be implemented by SW program. Another benefit is that we can shorten the development time period. In this paper, we propose and find its possibility as a new content protection and authentication method suitable for IPTV services.

• Journal of Advanced Navigation Technology
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• v.6 no.1
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• pp.44-51
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• 2002

This paper describes the performance evaluation and analysis of an Orthogonal Frequency-Division Multiplexing (OFDM) system having the least Inter Symbol Interference (ISI) in a multi-path fading channel environment. Wireless Local Area Network (W-LAN) in accordance with IEEE 802.11a and IEEE 802.11b provides high-speed transmission to universities, businesses and other various places. In addition, service providers can offer a public W-LAN service on restricted areas such as a subway. The proliferation of W-LAN has led to greater W-LAN service demands, but problems are also on the rise in offering a good W-LAN service. In particular, urban areas with high radio wave interference and many buildings are vulnerable to deteriorated QoS including disconnected data and errors. For example, when high-speed data is transmitted in such areas, the relatively high frequency generates ISI between Access Points (AP) and Mobile Terminals (such as a notebook computer), leading to a frequency selective fading channel environment. Consequently, it is difficult to expect a goodW-LAN service. The simulation proves that the OFDM system enables W-LAN to implement QoS in high-speed data transmission in a multi-path fading channel environment. The enhanced OFDM performance with 52 sub-carriers is verified via data modulation methods such as BPSK, QPSK and 16QAM based on IEEE 802.11a and punched convolutional codes with code rate of 1/2 and 3/4 and constraint length of 7. Especially, the simulation finds that the OFDM system has better performance and there is no data disconnection even in a mobile environment by applying a single tap equalizer and a decision feedback equalizer to a mobile channel environment with heavy fading influence. Given the above result, the OFDM system is an ideal solution to guarantee QoS of the W-LAN service in a high-speed mobile environment.

• Journal of Korean Neurosurgical Society
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• v.63 no.2
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• pp.178-187
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• 2020

Objective : The extensive vasa vasorum network functions as a conduit for the entry of inflammatory cells or factors that promote the progression of angiogenesis and plaque formation. Therefore, we investigated the correlation between the carotid vasa vasorum activities and carotid plaque vulnerability using indocyanine green video angiography (ICG-VA) during carotid endarterectomy (CEA). Methods : Sixty-nine patients who underwent CEA were enrolled prospectively from September 2015 to December 2017. During CEA, a bolus of ICG was injected intravenously before and after resecting the atheroma. Additionally, we performed immunohistochemistry using CD68 (a surface marker of macrophages), CD117 (a surface marker of mast cells), and CD4 and CD8 (surface markers of T-cells) antibodies to analyze the resected plaque specimens. Results : The density of active vasa vasorum was observed in all patients using ICG-VA. The vasa vasorum externa (VVE) and interna (VVI) were seen in 11 (16%) and 57 patients (82.6%), respectively. Macroscopically, the VVE-type patterns were strongly associated with preoperative angiographic instability (81.8%, p=0.005) and carotid plaque vulnerability (90.9%, p=0.017). In contrast, the VVI-type patterns were weakly associated with angiographic instability (31.6%) and plaque vulnerability (49.1%). CD68-stained macrophages and CD117-stained mast cells were observed more frequently in unstable plaques than in stable plaques (p<0.0001, p=0.002, respectively). Conclusion : The early appearance of VVE, along with the presence of many microvessel channels that provided nutrients to the developing and expanding atheroma during ICG-VA, was strongly associated with unstable carotid plaques. The degree of infiltration of macrophages and mast cells is possibly related to the formation of unstable plaques.

• International Journal of Contents
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• v.11 no.1
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• pp.41-51
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• 2015

Management systems for electronic library have been developed on the basis of Client/Server or ASP framework in domestic market for a long time. Therefore, both service provider and user suffer from their high cost and effort in management, maintenance, and repairing of software as well as hardware. Recently in addition, mobile devices like smartphone and tablet PC are frequently used as terminal devices to access computers through the Internet or other networks, sophisticatedly customized or personalized interface for n-screen service became more important issue these days. In this paper, we propose a new scheme of integrated management system for electronic library based on SaaS and Web Standard. We design and implement the proposed scheme applying Electronic Cabinet Guidelines for Web Standard and Universal Code System. Hosted application management style and software on demand style service models based on SaaS are basically applied to develop the management system. Moreover, a newly improved concept of duplication check algorithm in a hierarchical evaluation process is presented and a personalized interface based on web standard is applied to implement the system. Algorithms of duplication check for journal, volume/number, and paper are hierarchically presented with their logic flows. Total framework of our development obeys the standard feature of Electronic Cabinet Guidelines offered by Korea government so that we can accomplish standard of application software, quality improvement of total software, and reusability extension. Scope of our development includes core services of library automation system such as acquisition, list-up, loan-and-return, and their related services. We focus on interoperation compatibility between elementary sub-systems throughout complex network and structural features. Reanalyzing and standardizing each part of the system under the concept on the cloud of service, we construct an integrated development environment for generating, test, operation, and maintenance. Finally, performance analyses are performed about resource usability of server, memory amount used, and response time of server etc. As a result of measurements fulfilled over 5 times at different test points and using different data, the average response time is about 62.9 seconds for 100 clients, which takes about 0.629 seconds per client on the average. We can expect this result makes it possible to operate the system in real-time level proof. Resource usability and memory occupation are also good and moderate comparing to the conventional systems. As total verification tests, we present a simple proof to obey Electronic Cabinet Guidelines and a record of TTA authentication test for topics about SaaS maturity, performance, and application program features.