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99mTc-3PRGD2 SPECT/CT Imaging for Diagnosing Lymph Node Metastasis of Primary Malignant Lung Tumors

  • Liming Xiao;Shupeng Yu;Weina Xu;Yishan Sun;Jun Xin
    • Korean Journal of Radiology
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    • v.24 no.11
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    • pp.1142-1150
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    • 2023
  • Objective: To evaluate 99mtechnetium-three polyethylene glycol spacers-arginine-glycine-aspartic acid (99mTc-3PRGD2) single-photon emission computed tomography (SPECT)/computed tomography (CT) imaging for diagnosing lymph node metastasis of primary malignant lung neoplasms. Materials and Methods: We prospectively enrolled 26 patients with primary malignant lung tumors who underwent 99mTc-3PRGD2 SPECT/CT and 18F-fluorodeoxyglucose (18F-FDG) positron emission tomography (PET)/CT imaging. Both imaging methods were analyzed in qualitative (visual dichotomous and 5-point grades for lymph nodes and lung tumors, respectively) and semiquantitative (maximum tissue-to-background radioactive count) manners for the lymph nodes and lung tumors. The performance of the differentiation of lymph nodes with and without metastasis was determined at the per-lymph node station and per-patient levels using histopathological results as the reference standard. Results: Total 42 stations had metastatic lymph nodes and 136 stations had benign lymph nodes. The differences between metastatic and benign lymph nodes in the visual qualitative and semiquantitative analyses of 99mTc-3PRGD2 SPECT/CT and 18F-FDG PET/CT were statistically significant (all P < 0.001). The area under the receiver operating characteristic curve (AUC) in the semi-quantitative analysis of 99mTc-3PRGD2 SPECT/CT was 0.908 (95% confidence interval [CI], 0.851-0.966), and the sensitivity, specificity, positive predictive value, and negative predictive value were 0.86 (36/42), 0.88 (120/136), 0.69 (36/52), and 0.95 (120/126), respectively. Among the 26 patients (including two patients each with two lung tumors), 15 had pathologically confirmed lymph node metastasis. The difference between primary lung lesions in patients with and without lymph node metastasis was statistically significant only in the semi-quantitative analysis of 99mTc-3PRGD2 SPECT/CT (P = 0.007), with an AUC of 0.807 (95% CI, 0.641-0.974). Conclusion: 99mTc-3PRGD2 SPECT/CT imaging may notably perform in the direct diagnosis of lymph node metastasis of primary malignant lung tumors and indirectly predict the presence of lymph node metastasis through uptake in the primary lesions.

Black gram (Vigna Mungo L.) foliage supplementation to crossbred cows: effects on feed intake, nutrient digestibility and milk production

  • Dey, Avijit;De, Partha Sarathi;Gangopadhyay, Prabir Kumar
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.2
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    • pp.187-191
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    • 2017
  • Objective: An experiment was conducted to examine the effect of dietary supplementation of dried and ground foliage of black gram (Vigna mungo L.) on feed intake and utilization, and production performance of crossbred lactating cows. Methods: Eighteen lactating crossbred (Bos taurus${\times}$Bos indicus) cows (body weight $330.93{\pm}10.82kg$) at their second and mid lactation (milk yield $6.77{\pm}0.54kg/d$) were randomly divided into three groups of six each in a completely randomized block design. Three supplements were formulated by quantitatively replacing 0, 50, and 100 per cent of dietary wheat bran of concentrate mixture with dried and ground foliage of black gram. The designated supplement was fed to each group with basal diet of rice straw (ad libitum) to meet the requirements for maintenance and milk production. Daily feed intake and milk yield was recorded. A digestion trial was conducted to determine the total tract digestibility of various nutrients. Results: The daily feed intake was increased (p<0.05) with the supplementation of black gram foliage. Although the digestibility of dry matter, organic matter, crude protein, and ether extract did not vary (p>0.05), the fibre digestibility was increased (p<0.05), which ultimately improved (p<0.05) the total digestible nutrients content of composite diet. Although, the average milk yield (kg/animal/d) and composition did not differ (p>0.05) among the groups, milk yield was increased by 10 per cent with total replacement of wheat bran in concentrate mixture with of black gram foliage. The economics of milk production calculated as feed cost per kg milk yield (INR 10.61 vs 7.98) was reduced by complete replacement of wheat bran with black gram foliage. Conclusion: Black gram foliage could be used as complete replacement for wheat bran in concentrate mixture of dairy cows in formulating least cost ration for economic milk production in small holders' animal production.

Spatial Characteristics of Meiobenthic Community of Kongfjorden Sediment in the Svalbard Island, the Arctic Sea (북극해 스발바드 군도 Kongsfjorden 퇴적물에 서식하는 중형저서동물 군집의 공간 특성)

  • Kim, Dong-Sung;Shin, Jae-Chul;Kang, Sung-Ho;Chung, Ho-Sung
    • Ocean and Polar Research
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    • v.27 no.3
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    • pp.299-309
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    • 2005
  • The community structure of meiobenthos was studied in the sediment of Kongfjorden, Spitsbergen of Svalbard Island in the Arctic Sea. Samples of meiobenthos were collected in August, 2003. Meiobenthic organisms were collected by SCUBA and van veen grab or acryl sub-corers 34mm in internal diameter, and were taken from upper sediment to a depth of 3cm at each station. A total of 26 meiofaunal groups were found in the sediment of Spitsbergen in Svalbard Island. Nematodes were the most dominant faunal group. Sarcomastigophorans, benthic harpacticoids, and nauplius larvae of crustaceans, were also important components of the meiobenthic community of Kongsfjorden. All of these low faunal groups were comprised of more than 90% of total meiobenthos at every station. The total density of meiobenthos at each station was highest at station MeG 6 $(3,583{\pm}1,137inds./10cm^2)$, and lowest at station $MeG9(28{\pm}1inds./10cm^2)$. Meiobenthos in general showed the highest density in the upper 1cm layer. This may be associated with food and oxygen supply to subsurface. Harpacticoids showed extreme preference at the surface and little presence in layers deeper than 2cm. These animals may be less resistant to oxygen deficiency, and nauplius also showed the same trend. However, in St. MeG 8 and 9, meiobenthos were dense at depths of more than 0-1cm, at especially at depths of 2-3m because of relatively easy penetration of oxygen. Based on the results of cluster analysis, three meiobenthos assemblages were distinguished: one was in the outer and two were in the inner fjord. Station SCU 5 was grouped with the meiobenthos assemblage located in the outer fjord. The outer ford community was characterised by : 1) a relatively low mean number of meiobenthos taxa, 2) a relatively high density of harpacticods and nauplius. One of the inner ford communities (a group of four nation: MeG 2, 3, 8, 9) was in the proximity of the glaciers. Specifically, it was characterised by : 1) a low mean number of meiobenthos taxa, 2) a low density. The other inner ford community was characterised by both a high density and great mean number of meiofaunal taxa.

Preliminary Report on the Ecology of the Penguins Observed in the Cold Years and a Less Cold Year in the Vicinity of King Sejong Station, King George Island off the Antarctic Peninsula (남극 세종기지에서 추운 해와 덜 추운 해에 관찰된 펭귄들의 생태에 관한 1차보고)

  • Chang, Soon-Keun
    • Ocean and Polar Research
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    • v.26 no.1
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    • pp.87-101
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    • 2004
  • This paper delineated the ecology including movement (departure from the rookery and returning to the rookery), egg-laying, and hatch of the penguins occurred in the cold years and a less cold year in the vicinity of King Sejong Station, King George Island off the Antarctic Peninsula. The years of 1988, 1991, 1992, and 1995 were selected as cold years and the year of 2001 was selected as a less cold year based on the mean annual temperature of the years. Gentoo Penguin (Pygoscelis papua) left their rookery in May, meanwhile some remained around the station. They returned in middle-September in the less cold .year, and returned in late-September to early-October in the cold years. Chinstrap Penguin (Pygoscelis antarctica) left their rookery in early-April in the cold years as well as in the less cold year without exception. They returned to the rookery in late-October to early-November in cold years, meanwhile in early-October in the less cold year. This difference in the returning of this bird seems to be related with the exposed sea water, i.e., sea ice condition to feed in the sea. The global warming will lead to the appearance of birds which breed in the Sub-Antarctic. For example, one pair of King Penguin (Aptenodytes patagonicus) was observed in the Maxwell Bay in austral summer. And a pair of snide-like bird was recently observed for the first time in November 2001 at the penguin rookery located in the Barton Peninsula, King George Island. And it will also lead to the disappearance of an Emperor Penguin (Aptenodytes forsteri) which appeared in the full winter when Maxwell Bay and Marian Cove were frozen. It seems that the behaviour of the penguins observed around the station shows the complex effects of the ecology of the birds in combination with the natural environments, which include feeding strategy and areas, animal Instincts, exposed terrain related to weather conditions, and globa1 warming. It is necessary to take further observation and carry out systematic researches on the birds including penguins around the station which show the ecology of the birds as well as the environmental changes.

Comparative study on production, reproduction and functional traits between Fleckvieh and Braunvieh cattle

  • Cziszter, Ludovic-Toma;Ilie, Daniela-Elena;Neamt, Radu-Ionel;Neciu, Florin-Cristian;Saplacan, Silviu-Ilie;Gavojdian, Dinu
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.5
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    • pp.666-671
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    • 2017
  • Objective: Aim of the current comparative study was to evaluate production outputs, reproduction efficiency and functional traits in dual-purpose Fleckvieh and Braunvieh cows, reared under temperate European conditions. Methods: A data-set from 414 Fleckvieh and 42 Braunvieh cows and 799 lactations was analysed. ID tag number, milk yield per milking session, number of steps/interval and milk conductivity were recorded and collected daily using AfiMilk 3.076 A-DU software (Afimilk Ltd., Kibbutz, Israel). Production and milk quality data were taken from the results of the official performance recordings and the reproductive outputs of cows were recorded by the research stations veterinarians. Comparisons between the two genotypes were carried out using the one way analysis of variance protocol, with categorical factor being considered the breed of cows. All the statistical inferences were carried out using Statistica software (StatSoft Inc., Tulsa, OK, USA). Results: Fleckvieh cows significantly outperformed ($p{\leq}0.05$) the Braunvieh herd, with average milk yields of $5,252.1{\pm}35.79kg$ and $4,897.6{\pm}128.94kg$, respectively. Age at first calving was significantly ($p{\leq}0.01$) influenced by the breed, with Fleckvieh heifers being more precocious ($32.8{\pm}0.29mo$) compared to those of Braunvieh breed ($35.7{\pm}0.84mo$). Reproduction efficiency as defined by the number of inseminations per gestation, calving interval, dystocia, days dry and days open, was not influenced by genotype (p>0.05). Incidences of sub-clinical mastitis, clinical mastitis, lameness and abortions were not influenced by the breed factor (p>0.05). Stay-ability of cows was significantly ($p{\leq}0.001$) influenced by genotype, with Braunvieh cows having an average age at culling of $117.88{\pm}11.78$ months compared to $90.88{\pm}2.89$ months in Fleckvieh. Conclusion: Overall, results have shown that genotype significantly influenced milk yield, age at first calving and longevity.

Selection of Suitable Wild Herbs and Vegetables for Cultivation of Sub-highland (준고랭지역 재배 적정 산채류 선발)

  • Ahn, Song-Hee;Moon, Jung-Seob;Lee, Yong-Mun;Yang, Jin-Ho;Kim, Ju;Kim, Hee-Jun
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.04a
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    • pp.53-53
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    • 2018
  • 전라북도 동부권 지역은 중산악지형으로 표고 100~500m 산지가 69%, 500~1,000m가 26%로 이루어져 있어, 산채를 재배하기에 유리한 지리적 조건을 갖고 있다. 따라서 소비자의 수요가 높은 산채류의 재배 및 이용에 대한 농업인들의 기술개발이 요구되고 있다. 이에 전라북도 준고랭지역에서 재배하기 적합한 산채작목을 선발하고자 본 시험을 실시하였다. 시험은 표고 500m 전라북도 남원시 운봉읍 허브시험장에서 노지재배, 남원시 주천면 표고 530~540m, 진안군 주천면 표고 340~350m에 위치한 농가포장에서 임간재배를 실시하였다. 대상작물은 개미취(Aster tataricus), 갯기름나물(Peucedanum japonicum), 곤달비(Ligularia stenocephala), 눈개승마(Aruncus dioicus var. kamtschaticus), 단풍취(Ainsliaea acerifolia), 산마늘(Allium microdictyon), 섬쑥부쟁이(Aster glehnii), 어수리(Heracleum moellendorffii), 우산나물(Syneilesis palmata) 등 9종을 2016년 종묘를 정식하였다. 정식 2년차에 해당지역의 5월~9월 평균기온 및 조도 등 생육환경을 조사하고, 시험작물의 출현율, 생육특성, 수량 등을 조사하였다. 조사기간 동안의 평균기온은 노지가 $20.8^{\circ}C$, 남원임간은 $20.3^{\circ}C$, 진안임간은 $20.5^{\circ}C$이었으며, 평균조도는 노지가 37,362Lux, 남원임간은 7,816Lux, 진안임간은 25,316Lux이었다. 노지에서 출현율은 개미취, 갯기름나물, 눈개승마, 산마늘, 섬쑥부쟁이, 우산나물이, 임간에서는 개미취, 곤달미, 눈개승마, 단풍취, 산마늘, 어수리, 우산나물이 높았다. 생육특성은 노지에서 개미취, 갯기름나물, 눈개승마, 섬쑥부쟁이, 임간에서는 단풍취, 산마늘, 우산나물의 생육이 좋았다. 수량은 노지에서 개미취, 갯기름나물, 곤달비, 산마늘, 섬쑥부쟁이, 어수리가 높았으며, 남원임간에서는 곤달비, 단풍취, 우산나물, 어수리가, 진안임간에서는 개미취, 갯기름나물, 산마늘, 우산나물의 수량이 많았다. 이와 더불어 준고랭지에 적합한 산채류률 선발하기 위해서는 차후 재배 지역 및 방법의 차이에 따른 산채작물의 연차별 수량성과 경제성에 관한 연구가 이루어져야 할 것으로 여겨진다.

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Effects of Germination Condition, Nursery Media and Nutrient Concentration on Seedling Growth Characteristics of Pak-choi and Lettuce in Plant Factory (발아조건과 배지종류 및 양액 농도에 따른 식물공장 재배 청경채와 상추의 묘 생육특성)

  • Choi, Hyo-Gil;Kwon, Joon-Kook;Park, Kyoung-Sub;Kang, Yun-Im;Cho, Myeong-Whan;Rho, Il-Rae;Kang, Nam-Jun
    • Journal of Bio-Environment Control
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    • v.20 no.4
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    • pp.320-325
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    • 2011
  • This experiment was conducted to study the effects of germination condition, nursery media and nutrient concentration in plant factory on the growth characteristics of pak-choi and lettuce seedlings. The results showed that temperature (15, 20, $25^{\circ}C$) and light conditions (lighting and no lighting) had no significant effect on germination rate of pak-choi and lettuce, whereas the interaction of growing media (rockwool and urethane sponge) and moisture management before and after sowing had significant effect on germination percentage. In case of no moisture management before and after sowing, germination percentage was much higher in rockwool medium than urethane sponge medium. But germination percentage of urethane sponge was higher than that of rockwool with applying moisture before and after sowing. At the nutrient concentration of EC $1.0dS{\cdot}m^{-1}$ the fresh weight of pak-choi was heavier than in EC 1.5 and $0.5dS{\cdot}m^{-1}$ by 1.8 and 6.0 times, respectively. At the nutrient concentration of EC $1.0dS{\cdot}m^{-1}$ the fresh weight of lettuce was also heavier than in EC 1.5 and $0.5dS{\cdot}m^{-1}$ by 1.7 and 3.5 times, respectively.

Effects of Pregnant Rate after Embryo Transfer in Oxygen Consumption of Embryos in Korean Cattle (한우 수정란의 산소 소비량이 수청란이식 수태윷에 미치는 영향)

  • Choe, Chang-yong;Son, Jun-Kyu;Cho, Sang-Rae;Kang, Da-won;Yeon, Seong-Heum;Choi, Sun-Ho;Choi, Soo-Ho;Kim, Nam-Tae;Kim, Jae-Bum;Jung, Yeon-Sub;Kim, Sung-Jae;Jung, Jin-Woo;Bok, Nan-Hee;Yoo, Yong-Hee;Son, Dong-Soo
    • Journal of Embryo Transfer
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    • v.25 no.3
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    • pp.145-148
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    • 2010
  • Oxygen consumption has been regarded as a useful indicator for assessment of mammalian embryo quality. This study was performed to investigate whether oxygen consumption reflects morphological grade of in vivo derived bovine blastocyst-stage embryos (blastocyst). The oxygen consumption of in vitro produced blastocyst was compared to its total cell number. In addition, pregnant rate was measured after transplantation of in vivo blastocysts with different oxygen consumption. The quality of blastocyst collected on day 7 after artificial insemination was categorized as grade I and II (G I and G II) based on microscopic observation of the morphology. Oxygen consumption of blastocyst was measured using a scanning electrochemical microscopy (SECM) and total cell number of in vitro blastocyst was enumerated by counting cells stained by propidium iodide. Pregnancy of recipient cow was confirmed with rectal palpation after 60 days of embryo transfer. The oxygen consumptions of G I blastocysts were significantly higher than those of G II blastocysts ($10.2{\times}10^{15}/mol\;s^{-1}$ versus $6.4{\times}10^{15}/mol\;s^{-1}$, p<0.05). Total cell numbers of in vitro blastocysts were 74.8, 90.7, and 110.2 in the oxygen consumption of below 10.0, 10.0~12.0, and over $12.0{\sim}10^{15}/mol\;s^{-1}$ respectively. Total cell number was significantly increased in embryos with high oxygen consumption (p<0.05). Pregnant rate in recipient cow was 0, 50, and 85.7% in the transplantation of embryo with the oxygen consumption of below 10.0, 10.0~12.0, and over $12.0{\times}10^{15}/mol\;s^{-1}$, respectively. These results suggest that measurement of oxygen consumption may help increase the pregnant rate of bovine embryos.

Characteristics of Heavy Metals in the Industrial Complex Area of Pocheon City (포천시 공단지역 미세먼지 중 중금속농도 특성)

  • Shin, Hyung-Soon;Jung, Yeon-Hoon;Kim, Jin-gil;Jung, Jong-Pil;Lee, Sang-Soo;You, Han-Jo;Oh, Jo-Kyo
    • Journal of Environmental Health Sciences
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    • v.45 no.6
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    • pp.577-582
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    • 2019
  • Objectives: The purpose of this survey was to assess the concentrations of heavy metals in the atmosphere of Pocheon City by measuring heavy metals in the industrial complex area and at the city air measuring station, and also to assess the degree of impact that the industrial area has on urban air quality. Methods: Sampling was carried out between February 2018 and November 2018 at two sites in the industrial complex and in the city air monitoring stations. Results: At the industrial complex in Pocheon City, air pollutant emitting businesses were emitting concentrations of fine dust (PM10) between 45 and 60 ㎍/㎥ higher than in the city air. The daily maximum concentrations of lead (Pb), manganese (Mn), and cadmium (Cd) in the industrial complex are below the WHO recommendation standard (annual average), and the impact on the urban atmosphere is judged to be insignificant. Three to five percent of fine dust (PM10) consists of metallic materials, and as the fine dust increased, metals were detected proportionally. Although cadmium (Cd) and beryllium (Be) were not detected in the city air in Pocheon and chromium (Cr), copper (Cu), and arsenic (As) were found to be 50 percent or less, it is deemed that copper (Cu) was detected at unusually high levels due to unknown air pollutants, which requires regular heavy metal measurement and cause verification. Conclusions: An analysis of the heavy metals in the industrial zone and the urban atmosphere in Pocheon City in this study showed that the linear relationship of heavy metals in the industrial zone, or the direct impact relationship, on the heavy metals in the urban atmosphere could not be estimated. The sampling device for equivalent assessment of particulate matter installed at air pollution monitoring stations is highly likely to be used for analysis of fine dust and heavy metals.

Characteristic of room acoustical parameters with source-receiver distance on platform in subway stations (지하철 승강장의 음원-수음점 거리에 따른 실내음향 평가지수 특성)

  • Kim, Suhong;Song, Eunsung;Kim, Jeonghoon;Lee, Songmi;Ryu, Jongkwan
    • The Journal of the Acoustical Society of Korea
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    • v.40 no.6
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    • pp.615-625
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    • 2021
  • Prior to proposing appropriate standard for subway station platform, this study conducted field measurements to examine characteristics of room acoustics on platform of two subway stations. As a result of analyzing the longitudinal length of the platform, Sound Pressure Level (SPL) decreased (maximum difference : 14 dB), Reverberation Time (RT) tended to increase (maximum difference of 0.8 s ~ 1.5 s), and C50 and D50 were decreased (maximum difference: 5.9 dB ~ 9.1 dB and 31.8 % ~ 37.6 %, respectively) as measurement positions moved away from the sound source. The Interaural Cross-correlation Coefficient (IACC) did not show clear tendency, but it was lower than 0.3 in entire points. It is judged that the subway platform has non-uniform sound field characteristics due to various combinations of direct and reflective sound even though it is finished with a strong reflective material.This indicates that the room acoustic characteristics of the near and far sound field are clearly expressed depending on the source-receiver distances in the subway platform having a long flat shape with a low height compared to the length.Therefore, detailed architectural and electric acoustic design based on the characteristics of each location of speaker and sound receiver in the platform is required for an acoustic design with clear sound information at all positions of the platform.