• The Korean Journal of Mycology
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• v.42 no.4
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• pp.269-275
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• 2014

Halophytes of seven species, Carex scabrifolia Steud., Limonium tetragonum Bullock, Salicornia europaea L., Suaeda glauca Bunge, Suaeda japonica Makino, Suaeda maritima Dumort., and Triglochin maritimum L. were collected from the Taean Peninsula. Thirty-seven endophytic fungi were isolated from the root of halophytes, and analyzed using the DNA sequences of internal transcribed spacers (ITS). The diversity of all endophytic fungi was analyzed using diversity indices. The endophytic fungi associated with the halophytes belonged to seven orders; Eurotiales (78%), Capnodiales (5%), Hypocreales (5%), Agaricales (3%), Corticiales (3%), Glomerellales (3%), and Pleosporales (3%). At the phylum level, the endophytic fungi were composed of Ascomycota and Basidiomycota. At the genus level, Alternaria, Aspergillus, Cladosporium, Paecilomyces, Penicillium, Phanerochaete, Schizophyllum, Talaromyces, and Verticillium were confirmed. Among them, Penicillium was the most abundant in the roots of the halophytes. This study analyzed the distribution and diversity of endophytic fungi on halophytes in the Taean Peninsula.

• Journal of Microbiology and Biotechnology
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• v.31 no.11
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• pp.1526-1532
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• 2021

Suaeda australis, Phragmites australis, Suaeda maritima, Suaeda glauca Bunge, and Limonium tetragonum in the Seocheon salt marsh on the west coast of the Korean Penincula were sampled in order to identify the endophytes inhabiting the roots. A total of 128 endophytic fungal isolates belonging to 31 different genera were identified using the fungal internal transcribed spacer (ITS) regions and the 5.8S ribosomal RNA gene. Fusarium, Paraconiothyrium and Alternaria were the most commonly isolated genera in the plant root samples. Various diversity indicators were used to assess the diversity of the isolated fungi. Pure cultures containing each of the 128 endophytic fungi, respectively, were tested for the plant growth-promoting abilities of the fungus on Waito-C rice germinals. The culture filtrate of the isolate Lt-1-3-3 significantly increased the growth of shoots compared to the shoots treated with the control. Lt-1-3-3 culture filtrate was analyzed and showed the presence of gibberellins (GA₁ 2.487 ng/ml, GA₃ 2.592 ng/ml, GA₉ 3.998, and GA₂₄ 6.191 ng/ml). The culture filtrate from the Lt-1-3-3 fungal isolate produced greater amounts of GA₉ and GA₂₄ than the wild-type Gibberella fujikuroi, a fungus known to produce large amounts of gibberellins. By the molecular analysis, fungal isolate Lt-1-3-3 was identified as Gibberella intermedia, with 100% similarity.

• Korean Journal of Environmental Agriculture
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• v.22 no.2
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• pp.79-86
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• 2003

This research was conducted to present reference data to be used as newly reclaimed tidal land management. We investigated vegetation succession at 4 reclaimed/reclaiming project areas and discussed relationship with soil and vegetation trhrough investigation and analysis soil chemical characteristics at 2 areas. 14 families 58 kinds were investigated. Vegetation were variou at Dea-Ho conservation polt and Seok-Mun National Industrial Area which are maintaining naturally. Vegetation were simple at Hong-Bo and Dongjin and MinKyong river areas which effected sea water. Common species that were investigated at 9 sites were Suaeda asparagoides, Aster tripolium, Phragmites australis, Suaeda maritima, Suaeda japonica, Carex scabrifolis. As soil desalinization progressing, soil classified at first saline-soidc soil, the nest saline soil and then normal soil. Chenopodiaceae revealed at about 30 dS/m of soil ECe and existed to 10 dS/m of soil ECe. At about 20 dS/m of soil ECe. Aster tripolium, Calamagrostis epigeios, and Sonchus brachyotus revealed and then non-halophytes and common plants at inland revealed at low soil ECe of about 10 dS/m. However it was not to progress vegetation sucdession and soil desalinization at the same time, owing to input of seeds or plants ect from out-ecosystem. So for promotion of vegetation at newly reclaimed tidal land, we proposed that it was very effective to plant artificially halophytes or suitable species through soil test.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.24 no.1
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• pp.139-159
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• 2019

This survey was conducted from May 2017 to August 2018 as part of the National Marine Ecosystem Surveys sponsored by MOF (Ministry of Oceans and Fisheries) and KOEM (Korea Marine Environment Management Corporation). The survey was conducted at 157 stations including intertidal zones, coastal sand dunes and beaches. A total of 72 species of halophytes were identified and the total vegetated area was $1,813,546m^2$ in coastal waters of Korea. In the west coast region, Suaeda japonica was the dominant species, Phragmites communis in the southern coast, and Carex kobomugi in the east coast. Phragmites communis was the most dominant with $1,136,643m^2$ (62.7%) in the whole surveyed area. The top 11 dominant plants are Phragmites communis, Suaeda japonica, Suaeda maritima, Zoysia sinica, Carex kobomugi, Elymus mollis, Suaeda malacosperma, Phacelurus latifolius, Triglochin maritimum, Carex pumila and Carex scabrifolia, and Phragmites communis and Suaeda japonica accounted for 75.9% of the total vegetated area. The distribution of halophyte on the coast of Korea was determined by the habitat types rather than the climate ranges, and there was a difference in the species diversity and habitat range of the halophyte depending on the habitat diversities within the same area.

• The Korean Journal of Mycology
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• v.40 no.2
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• pp.86-92
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• 2012

Halophyte samples, such as Suaeda japonica, Phragmites australis, Limonium tetragonum, Suaeda maritima were collected from Go-chang salt marsh. Thirty-nine endophytic fungal strains were isolated from the roots of halophytes naturally growing in salt marsh. All endophytic fungal strains isolated were analyzed by internal transcribed spacer (ITS) containing ITS1, 5.8 s and ITS2 region. Endophytic fungal strains belong to eight orders, i.e., Eurotiales (36%), Pleosporales (26%), Hypocreales (18%), Incertae sedis (8%), Glomerellales (5%), Sordariales (2%), Xylariales (2%), and Capnodiales (3%). On genus level, they were composed of Alternaria, Aspergillus, Chaetomium, Cladosporium, Colletotrichum, Coniothyrium, Dothideomycete, Fusarium, Gibberella, Macrophoma, Penicillium, Pestalotiopsis, Phaeosphaeria, Phoma, Pleosporales, Pseudozyma, Talaromyces, and Termitomyces. Of them, Penicillium (26%), Fusarium (13%) of Eurotiales and Hypocreales were predominant.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.39 no.spc1
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• pp.180-188
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• 2006

We examined the effect of salt marsh vegetation on the benthic environment in macro-tidal flats at Donggeomdo, Ganghwa, on the west coast of Korea. Nine stations were established along a transect across the tidal flats, including salt marsh, and field sampling was conducted monthly from July 1997 to June 1998. During the study period, environmental parameters fluctuated as follows: salinity, 15.0 to 28.2 psu, exposure time, 613 to 702 hr/m, inundation time, 28 to 117 hr/m, sediment organic carbon, 0.71 to 1.34%, nitrogen, 0.07 to 0.16%, sulfur from 0.07 to 0.22%, mean grain size from 6.3 to $6.9{\phi}$, water content from 19.4 to 44.4%, water temperature from 4.4 to $20.4^{\circ}C$ and temperature of the surface sediments, 2.7 to $31.1^{\circ}C$, in total, seven halophyte species were sampled and found to be vertically distributed across the tidal levels. Carer scabrifolia, Suaeda maritima, Limonium tetragonum and Phragmites communis dominated the upper zone of the salt marsh, Juncus haenkei, Triglochin maritimum and P. communis dominated the middle zone, and Suaeda japonica predominated in the lower area. Principal component analysis (PCA) and non-metric multi-dimensional scaling revealed that the zonation of halophytes was related to environmental variables such as salinity and exposure time. The halophyte communities were likely related to the organic content of the surface sediment.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.4 no.3
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• pp.66-74
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• 2001

The main purpose of this thesis is to select the appropriate plants for the ecological restoration of coastal land. The soil and vegetation environment survey of the beach where the halophytes live now was executed for the sake. Firstly, the soil survey was done by the physical and chemical survey of beach soil and dumped soil. The soil is mostly sandy loam and sandy clay loam which has silty much. The beach soil where the salt plants live has more organic matters in the content than dumped soil because of the influx of the dead halophytes, a pioneer plant to this area. Secondly the vegetation survey was executed by the halophytes survey in the beach area in order to select the appropriate plants for ecological restoration. The shows two different areas in the vegetation divided by the salt density of soil. Artemisia fukudo, Juncus haenkei, Carex pumila, Silene armeia, Polyponum aviculare etc live in the less salt density area and it doesnt show collected. Salicornia herbacea, Suaeda asparagoides, Aster tripolium, Limonium tetragonum, Suaeda maritima, Scirus fluvitilis, Phragmtes communis, Suaeda japonica, Zoysia sinica etc halophytes live in the more salt density area and there are a few advent plant but many collected. So the salt density of soil limits the vegetation. The selection of appropriate plants for the ecological restoration programs was designed with 3 different standards considering the salt density of soil in the dumped soil area. The plan selection guidelines of thick salt density due to the salt density of soil, and economical efficiency and maintenance because the area is large. The guidelines of middle salt density area were made considering economical efficiency and maintenance because there are salt as well, and it is effective for the scenery if they have normal ecology but their leaves have good color and the flowers are beautiful. However I suggest beautiful flowery plants for the neutral plan area because this area is the entry point of the airport so the view is very important.

• Korean Journal of Environmental Agriculture
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• v.17 no.1
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• pp.26-33
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• 1998

This experiment was conducted to classify the plant species occurring in the saline reclaimed land by saline tolerance. The vegetation of reclaimed land was composed of various plant species from halophyte to glycophyte showing different saline tolerances. In the investigated saline region, reclaimed land of Youngjong island, Inchun city, 175 species belonging to 32 families were found. Our survey was carried out in two region, having different salinity due to different desalinization. The electricalconductivity (EC) of more saline region showed 48.0mS/cm nd the other region showed 13.0mS/cm. It is assumed that intensity of precipitation and runoff of rainfall may cause salinity gradient in the investigated region. The plant species occurred in the experimental region were classified as 72 species of annual, 42 species of biennial, and 61 species of perennial according to life cycle. For knowing relationship between vegetation of saline region and saline tolerance of occurring species, we tested the saline susceptibility of plant species collected at the saline regions. Testing plants were cultured by nutrient solution containing 200 mM NaCl, the critical concentration of survival in glycophytes. The saline tolerance was graded by the growing capacity in the sand-culture system. The more saline-tolerant species screened by sand culture were Atriplex gmelini, Suaeda asparagoides, Aster tripolium, Suaeda maritima, Salicornia herbacea, and Suaeda japonica. The most saline tolerant family was Chenopodiaceae. Poaceae, Cyperaceae, and Brassicaceae showed relatively high tolerance to saline stress. In the course of growth under the high saline condition, the most noticeable change was the darkening of leaves by increasing of chlorophyll content. The chlorophyll contents were increased with saline stress in most species.

• Mycobiology
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• v.43 no.4
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• pp.373-383
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• 2015

Five halophytic plant species, Suaeda maritima, Limonium tetragonum, Suaeda australis, Phragmites australis, and Suaeda glauca Bunge, which are native to the Muan salt marsh of South Korea, were examined for fungal endophytes by sequencing the internal transcribed spacer (ITS) region containing ITS1, 5.8S rRNA, and ITS2. In total, 160 endophytic fungal strains were isolated and identified from the roots of the 5 plant species. Taxonomically, all 160 strains belonged to the phyla Ascomycota, Basidiomycota, and Zygomycota. The most dominant genus was Fusarium, followed by the genera Penicillium and Alternaria. Subsequently, using 5 statistical methods, the diversity indices of the endophytes were determined at genus level. Among these halophytic plants, P. australis was found to host the greatest diversity of endophytic fungi. Culture filtrates of endophytic fungi were treated to Waito-C rice seedlings for plant growth-promoting effects. The fungal strain Su-3-4-3 isolated from S. glauca Bunge provide the maximum plant length (20.1 cm) in comparison with wild-type Gibberella fujikuroi (19.6 cm). Consequently, chromatographic analysis of the culture filtrate of Su-3-4-3 showed the presence of physiologically active gibberellins, $GA_1$ (0.465 ng/mL), $GA_3$ (1.808 ng/mL) along with other physiologically inactive $GA_9$ (0.054 ng/mL) and $GA_{24}$ (0.044 ng/mL). The fungal isolate Su-3-4-3 was identified as Talaromyces pinophilus.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.28 no.3
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• pp.305-309
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• 1983

To know the effect of desaltation in the reclaimed tidal fields on the succession of vegetation and on the root hair formation of paddy rice, reclaimed fields in Kang Hwa Island, Nam-Yang Bay and Ke-Hwa Island were investigated during summer crop season in 1982. The obtained results can be summarized as followings. l) In the highly salty and water logged areas of reclaimed tidal fields, the first dominating species of higher plant were observed to be Salicornia herbacea L. and Suaeda japonica Makino. With the continued desaltation, Chemopodium virgatum Thumb. occurred in the vegetation. After this transition, the dominating species were composed of Scirpus maitimus L. and Phragmites communis Trin. At the S. maitimus and P. communis dominating salinity level, rice cultivation was safe from the salt damage. 2) In the water logged area, Artemisia capillaria Thunb. and Aster tripolium L. took the place of dominating species after S. herbacea and S. japonica. At this salinity level, graminaceous weeds began to immigrate. 3) In dry areas, Suaeda asparagoides Makino and Suaeda maritima were the first appearing dominant species. Atriplex subcordata Kitakawa was also observed in sucy dry areas, but colony formation was not observed. 4) Plants immigrated slowly into dry areas from the already vegetated water logged areas with the continuation of desaltation. 5) The high soil salinity level affected the root hair formation of rice by reducing both the rate of root hair formation and the length of root hairs.