• Korean Journal of Microbiology
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• v.27 no.3
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• pp.194-200
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• 1989

The nucleotide sequence of inducible chloramphenicol acetyl-transferase(CAT) gene isolated from a small plasmid pSBK203 of Staphylococcus aureus was determined. The base sequence shows that structural gene of pSBK203-CAT encodes a protein of 213 amino acids and has a leader region which encodes a short polypeptide of 9 amino-acids in its upstream. vertical bar /sup 35/S vertical bar-Methionine labelled CAT gene product in minicell showed almost same mobility with pC194-CAT of which molecular weight is 24Kdal on polyacrylamide gel electrophoresis. Predicted amino acid sequence of pSBK203-CAT has revealed a high degree of homology with the CATs of pC194 and pC221 than those of cat-86, Tn9 and proteus mirabilis PM13.

• Macromolecular Research
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• v.12 no.2
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• pp.178-188
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• 2004

We performed molecular dynamics (MD) simulations at 300 K on a series of poly(benzyl ether) (PBE) dendrimers having a different core functionalities. We used the rotational isomeric state Metropolis Monte Carlo (RMMC) method to construct the initial configuration in a periodic boundary cell (PBC) before the MD simulations were undertaken. To elucidate the effects that the structural features have on the chain dimension, the overall internal structure, and the morphology, we monitored the radii of gyration, R$\sub$g/ and the conformational changes during the simulations. The PBE dendrimers in a glassy state adopted less-extended structures when compared with the conformations obtained from the RMMC calculations. We found that R$\sub$g/ of the PBE dendrimer depends on the molecular weight, M, according to the relation, R$\sub$g/∼M$\^$0.22/. The radial distributions of the dendrimers were developed identically in the PBC, irrespective of the core functionality. A gradual decrease in radial density resulted from the fact that the terminal branch ends are distributed all over the molecule, except for the core region.

• Journal of Plant Biology
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• v.39 no.3
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• pp.173-177
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• 1996

A novel calcium binding protein (CaBP) was purified to electrophoretic homogeneity from Dunaliella salina. In the course of purification experiment, this CaBP was identified as a monomer and its molecular weight was about 21 kDand isoelectric point (pI) value was about 4.1 using isoelectrofocusing. This CaBP was able to bind Ca2+ even in the pressence of an excess MgCl2 and KCI both in solution. In the SDS-PAGE, the Ca2+-bound form was slower than the Ca2+-free form in the nondenaturing PAGE. This means that the CaBP undergoes conformational change in the Ca2+-bound condition. Furthermore, UV absorption spectrum and fluorescence intensity of this CaBP was investigated. UV absorption peak was appeared at about 258 nm and decreased somewhat in Ca2+-bound condition. In the measurement of fluorescence, maximum intensity was appeared at 303 nm and decreased in Ca2+-bound state, similarly as UV absorption spectrum. These show distinct changes upon Ca2+-binding, which indicate of structural and/or dynamic changes largely reminiscent of other members of the EF-hand Ca2+-binding protein family.

• Bulletin of the Korean Space Science Society
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• 2011.04a
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• pp.31.2-31.2
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• 2011

IGRINS (Immersion GRating INfrared Spectrometer) is a high resolution wide-band infrared spectrograph developed by Korea Astronomy and Space Science Institute (KASI) and the University of Texas at Austin (UT). The slit-viewing camera is one of four re-imaging optics in IGRINS including the input relay optics and the H- and K- band spectrograph cameras. Consisting of five lenses and one Ks-band filter, the slit viewing camera relays the infrared image of $2'{\times}2'$ field around the slit to the detector focal plane. Since IGRINS is a cryogenic instrument, the lens barrel is designed to be optimized at the operating temperature of 130 K. The barrel design also aims to achieve easy alignment and assembly. We use radial springs and axial springs to support lenses and lens spacers against the gravity and thermal contraction. Total weight of the lens barrel is estimated to be 1.2 kg. Results from structural analysis are presented.

• Journal of the Korea Concrete Institute
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• v.17 no.1 s.85
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• pp.149-155
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• 2005

The purpose of this study is to investigate the flexural strengthening effects of CF(Carbon Fiber) sheet for the full-scale RC beams with multi-layer CF sheets. The partial strength reduction factors of CF sheets are suggested from the full-scale RC beams tests strengthened with multi-layer CF sheets up to six layers as well as material tests. From the material tensile tests, it was observed that the average tensile strengths of CF sheets per layer are decreased as the number of CF sheets is increased. Also the steep strength reductions of CF sheets in material test results at rupture are observed compared with the structural tests results for the full-scale RC beams strengthened with multi-layer CF sheets. Finally, the partial strength reduction factors far CF sheets up to six layers are suggested considering the effects of multi-layer and unit weight of CF sheets.

• International Journal of Concrete Structures and Materials
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• v.10 no.sup3
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• pp.43-52
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• 2016

The degradation of the load-bearing capacity of reinforced concrete beams due to corrosion has a profoundly negative impact on the structural safety and integrity of a structure. The literature is limited with regard to models of bond characteristics that relate to the reinforcement corrosion percentage. In this study, uniaxial tensile tests were conducted on specimens with irregular corrosion of their reinforced concrete. The development of cracks in the corroded area was found to be dependent on the level of corrosion, and transverse cracks developed due to tensile loading. Based on this crack development, the average stress versus deformation in the rebar and concrete could be determined experimentally and numerically. The results, determined via finite element analysis, were calibrated using the experimental results. In addition, bond elements for reinforced concrete with corrosion are proposed in this paper along with a relationship between the shear stiffness and corrosion level of rebar.

• Journal of Ocean Engineering and Technology
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• v.17 no.6
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• pp.38-46
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• 2003

Aim of this article is to propose Micro-Genetic Simulated Annealing (${\mu}GSA$) as a hybrid metaheuristics approach to find the global optimum of nonlinear optimisation problems. This approach combines the features of modern metaheuristics such as micro-genetic algorithm (${\mu}GAs$) and simulated annealing (SA) with the general robustness of parallel exploration and asymptotic convergence, respectively. Therefore, ${\mu}GSA$ approach can help in avoiding the premature convergence and can search for better global solution, because of its wide spread applicability, global perspective and inherent parallelism. For the superior performance of the ${\mu}GSA$, the five well-know benchmark test functions that were tested and compared with the two global optimisation approaches: scatter search (SS) and hybrid scatter genetic tabu (HSGT) approach. A practical application to structural sandwich panel is also examined by optimism the weight function. From the simulation results, it has been concluded that the proposed ${\mu}GSA$ approach is an effective optimisation tool for soloing continuous nonlinear global optimisation problems in suitable computational time frame.

• Journal of Advanced Research in Ocean Engineering
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• v.2 no.1
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• pp.28-39
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• 2016

This paper provides theoretical and experimental results to verify the crashworthiness of FH32 high-strength steel for arctic marine structures against ice impact. Assuming that side-shell structures of the Korean arctic research vessel, ARAON, with ice-notation PL10, collide with sheet ice, one-third-scale test specimens with a single transverse frame are manufactured. Impact-bending tests were conducted using a rigid steel striker that mimics sheet ice. Drop height was calculated by considering the speed at which sheet ice is rammed. Prior to impact-bending tests, tensile coupon tests were conducted at various temperatures. The impact-bending tests were carried out using test specimens fully fixed to the inside bottom frame of a cold chamber. The drop-weight velocity and test specimen deformation speed were measured using a high-speed camera and digital image correlation analysis (DICA). Numerical simulations were carried out under the same conditions as the impact-bending tests. The simulation results were in agreement with the test results, and strain rate was a key factor for the accuracy of numerical simulations.

• Proceedings of the Korean Operations and Management Science Society Conference
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• 2001.10a
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• pp.111-115
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• 2001

A good business performance measurement system is an effective tool io sustained growth in profits. Although interest in creating performance measurement models is widespread, a well-designed system is rare. To be successful in today's competitive environment, a performance measurement system should incorporate strategic success factors and contain financial and non-financial measuring index to carry out strategic management. In the 1990s, Kaplan & Norton introduced a concept called the Balanced Scorecard. The Balanced Scorecard supplements traditional financial measures with criteria that measured performance from three additional perspectives - those perspectives of customers, internal business processes, and learning and growth. This paper presents five measuring index criteria for each perspective. To calculate the relative priority for These measuring index, we investigate weights investigated by interviews with management consultant. Then, AHP method is employed for calculating priority weight. Our evaluation model may be referred to as the Balanced Analytic Hierarchical Performance Model(BAHPM) in the sense that the analytic hierarchical scheme, along with the AHP, is applied. The BAHPM is the first kind of analytical model to cover a wide variety of measures. In comparison with previous evaluation models, our model shows strengths in structural flexibility, ease of incorporating feedback, group evaluation capacity, participation promotion, sensitivity analysis, and computational simplicity. A prototype based on the BAHPM can be applied to various industry sectors.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2008.04c
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• pp.50-53
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• 2008

In this work, the $TiO_2$ pigment influence in HDPE dielectric strength was analyzed. Chemical and structural characterizations were made to identify changes during the processing and your influence in the electrical properties. Formulations containing 0, 0.5, 1, 2.5, 4 and 6 of titanium dioxide were processed by extrusion and injection molding with stabilization-antioxidants, ultraviolet stabilizers and plasticizers. The electrical strength tests were analyzed by the statistical distribution of Weibull, and the maximum likelihood method. The high concentrations present lower values to electrical strength. The $\beta$ parameter could be using to insulator particles dispersion. The $TiO_2$ concentration variation shows that these incorporations implicate strength values increase has a maximum (5,35MV/cm). High pigment concentration induces a little falls in property values. Observing the $\beta$ parameter, minimum experiment electric field (Ebmin) and electric strength value, found that the best electric perform formulation was the formulation with 2.5% $TiO_2$ weight.