• Animal cells and systems
• /
• v.8 no.3
• /
• pp.205-212
• /
• 2004

The tumor suppressor protein p53 is stabilized by the herpes-virus-associated ubiquitin-specific protease (HAUSP), a deubiquitinating enzyme. We previously isolated and characterized a mouse orthologue of HAUSP, mHAUSP. mHAUSP cDNA consisted of 3,312 bp encodes 1,103 amino acids with a molecular weight of approximately 135 kDa containing highly conserved Cys, Asp (I), His, and Asn/Asp (II) domains. In this study, we carried out site-directed mutagenesis of 6 conserved amino acids (Cys224, Gln231, Asp296, His457, His465, and Asp482) in Cys box, QQD box, and His box. Interestingly, the conserved Gln 231 was not essential for the catalytic activity of mHAUSP. However, the other conserved amino acids were required for deubiquitinating activity of mHAUSP. We performed isopeptidase assay and confirmed that mHAUSP is able to remove ubiquitin from ubiquitinated substrates. In addition, we observed that mHAUSP induces apoptosis in HeLa cells.

• Food Science and Biotechnology
• /
• v.14 no.4
• /
• pp.470-473
• /
• 2005

Starch was isolated from turnip (Brassica rapa L.), and to elucidate the structure-function relationship its structural and physical properties were characterized. Morphological structure of the starch was analyzed by SEM (Scanning Electron Microscopy). Most of the starch granules were spherical in shape with diameter ranging from 0.5-10mm. Apart from larger granules ($<10\;{\mu}m$) which dominated the population size of turnip starch, significant amount of small ($0.5-2\;{\mu}m$) and mid-size granules (${\sim}\;{\mu}m$) were also detected. It was revealed that presumably, erosion damages occurred due to the attack of amylase-type enzymes on the surface of some granules. Branch chain-length distribution was analyzed by HPAEC (High-Performance Anion-Exchange Chromatography). The chain-length distribution of turnip starch revealed a peak at DP12 with obvious shoulder at DP18-21. The weight-average chain length ($CL_{avg}$) was 16.6, and a large proportion (11.8%) of very short chains (DP6-9) was also observed. The melting properties of starch were determined by DSC (Differential Scanning Calorimetry). The onset temperature ($T_o$) and the enthalpy change (${\Delta}H$) of starch gelatinization were $50.5^{\circ}C$ and 12.5 J/g, respectively. The ${\Delta}H$ of the retrograded turnip starch was 3.5 J/g, which indicates 28.2% of recrystallization. Larger proportion of short chains as well as smaller average chain-length can very well explain relatively lower degree of retrogradation in turnip starch.

• BMB Reports
• /
• v.42 no.9
• /
• pp.605-610
• /
• 2009

Plasma high-density lipoprotein cholesterol (HDL-C) levels are inversely correlated with the risk of cardiovascular disease, and are known to increase with repetitive exercise. In the current study, HDL fractions from athletes' sera were isolated and compared as a function of the type of sport (runners [n = 10], throwers [n = 10], wrestlers [n = 10], and weight lifters [n = 8]), and as an age- and gender-matched reference group (n = 14). Among athletes, HDL from runners had the strongest antioxidant activity. Immunodetection showed that runners and wrestlers had the highest levels of apoA-I and lowest levels of apoA-II in their HDL. Electron microscopy also revealed that HDL2 of runners and wrestlers were the largest in size. In conclusion, although all athlete groups had significantly better serum lipid/lipoprotein profiles than the reference group, runners and wrestlers had the most desirable lipoprotein function and structure, including antioxidant activity, HDL-associated enzyme activities and increased particle size.

• Journal of the Korea Institute of Building Construction
• /
• v.8 no.2
• /
• pp.63-70
• /
• 2008

Reinforcing steel work plays an important role in terms of its structural performance or weight of construction cost for reinforced concrete structures. Precise estimation of re-bar quantity gives a basis for managing the reinforcing steel work effectively. However, the estimation process is still performed ineffectively based upon the expert's experience or manpower in spite of the advanced technology or improvement efforts. Therefore, the purpose of this research is to develop a prototype system for taking-off the quantity of reinforcing steel bars quickly and accurately in an order consistent with the specific members identified on the drawings. An estimate algorithm considering the connection, settlement and coating thickness of re-bars was suggested regarding to their replacement conditions which places more emphasis on constructibility. Also, this system produces the shop drawings automatically with the calculation results with work zone information.

• Proceedings of the KSR Conference
• /
• 2011.05a
• /
• pp.697-706
• /
• 2011

There are more than 800 railway steel plate girder bridges which are in use and the total length is approximately 50 km. Among these, it shall be pointed out that non-ballast rail systems which lay on wood sleepers are the most critical members. To strengthen this type of structures, mainly two methods have been applied. The first one is the most typical method which is to replace the girders with slab girder system or steel composite girders and to add ballast. It is not uncommon that the construction cost of substructure is more than ten time higher than that of superstructures and even in this case, the structural uncertainty for the substructures is not diminished. To resolve above mentioned problems, new method was developed to rehabilitate railway steel girder bridge by adding PC-slab using transport equipment. Using this method, substructure strengthen is rarely required because the additional weight to the bridge superstructure is only up to 1.0t/m. Also it was possible to save the construction cost by reducing construction duration and by simplifying the construction process. Experimental construction was performed for Jewon bridge and measurements were performed before and after construction to verify the bridge capacity.

• Structural Engineering and Mechanics
• /
• v.16 no.5
• /
• pp.557-580
• /
• 2003

Laminated composite structures find wide range of applications in many branches of technology. They are much suited for weight sensitive structures (like aircraft) where thinner and lighter members made of advanced fiber reinforced composite materials are used. The orientations of fiber direction in layers and number of layers and the thickness of the layers as well as material of composites play a major role in determining the strength and stiffness. Thus the basic design problem is to determine the optimum stacking sequence in terms of laminate thickness, material and fiber orientation. In this paper, a new optimization technique called Cellular Automata (CA) has been combined with Genetic Algorithm (GA) to develop a different search and optimization algorithm, known as Cellular Genetic Algorithm (CGA), which considers the laminate thickness, angle of fiber orientation and the fiber material as discrete variables. This CGA has been successfully applied to obtain the optimal fiber orientation, thickness and material lay-up for multi-layered composite hybrid beams plates and shells subjected to static buckling and dynamic constraints.

• Structural Engineering and Mechanics
• /
• v.30 no.3
• /
• pp.369-382
• /
• 2008

An oscillator of two lumped masses linked through a vertical spring moves forward in the horizontal direction, initially at a certain height, over a horizontal Euler beam and descends on it due to its own weight. Vibration of the beam and the oscillator is excited at the onset of the ensuing impact. The impact produced by the descending oscillator is assumed to be either perfectly elastic or perfectly plastic. If the impact is perfectly elastic, the oscillator bounces off and hits the beam a number of times as it moves forward in the longitudinal direction of the beam, exchanging its dynamics with that of the beam. If the impact is perfectly plastic, the oscillator (initially) sticks to the beam after its first impact and then may separate and reattach to the beam as it moves along the beam. Further events of separation and reattachment may follow. This interesting and seemingly simple dynamic problem actually displays rather complicated dynamic behaviour and has never been studied in the past. It is found through simulated numerical examples that multiple events of separation and impact can take place for both perfectly elastic impact and perfectly plastic impact (though more of these in the case of perfectly elastic impact) and the dynamic response of the oscillator and the beam looks noisy when there is an event of impact because impact excites higher-frequency components. For the perfectly plastic impact, the oscillator can experience multiple events of consecutive separation from the beam and subsequent reattachment to it.

• Structural Engineering and Mechanics
• /
• v.47 no.4
• /
• pp.545-557
• /
• 2013

Disposal of construction wastes poses major challenge to the municipal administration in the developing countries. At the same time new developments in these countries are unscrupulously exploiting the natural resources. The sustainable development requires judicious and careful utilization of natural resources. In this context, reuse of construction and demolition waste can save the global natural resources to greater extent. In this work the bricks and concrete waste from construction sites were crushed to the desired sizes and mixed in various proportions to study its properties in the concrete both in fresh and hardened states. Six mixes of natural and recycled aggregates were used to make the coarse aggregates for the concrete. From each mix nine cylinders were cast, which were tested at 7,14 and 28 days. The properties of concrete with recycled aggregates were compared with the control mix having natural aggregates. The nominal ratio of cement sand and coarse aggregates were kept at 1:2:4 by weight for all mixes. The tests have shown that concrete with recycled aggregates made from old concrete and brick bats provide greater opportunities for reuse of construction wastes in concrete.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.31 no.10
• /
• pp.1025-1030
• /
• 2007

Three kinds of metallic sandwich panels with quasi-Kagome truss cores have been analyzed on their mechanical behaviors subjected to bending load. According to the results of previous work on the optimal design, they were designed to have similarly high strength per weight with the identical overall sizes, i.e., the total length, the width, the core height. Differences were in the face sheet thickness and/or the thickness of the metal sheet from which the core was fabricated through expanding and bending processes. Under the bending load, they performed well as designed, as far as the maximum load is concerned. However, after the maximum load, the load-displacement curves were different each other depending on the slenderness ratio of the truss elements composing the quasi-Kagome truss cores and the face sheet thickness. Namely, the slenderness ratio and the face sheet thickness governed stability of the elastic and plastic buckling. Therefore, if energy absorption characteristics or structural stability as well as the maximum load capacity are to be achieved, the sandwich panel with thick truss members and thick face sheet should be selected.

• Computers and Concrete
• /
• v.26 no.4
• /
• pp.317-325
• /
• 2020

A precise estimation of the natural time period of buildings improves design quality, causes a significant reduction of the buildings' weight, and eventually leads to a cost-effective design. In this study, in order to optimise the reinforced concrete frames design, some symmetrical and unsymmetrical buildings composed of solid and hollow members have been simulated using finite element software SAP 2000. In numerical models, different parameters such as overturning moment, story drift, deflection, base reactions, and stiffness of the buildings were investigated and the results have been compared with strength and serviceability limit criteria proposed by Australian Standard (AS 3600 2018). Comparing the results of the numerical modelling with existing standards and performing a cost analysis proved the merits of hollow box sections compared to solid sections. Finally, based on numerical simulation results, two equations for natural time period of moment resisting reinforced concrete buildings have been presented. Both derived equations reflected higher degree of correlation and reliability with different complexities of building when compared with existing standards and relationships provided by other scholars. Therefore, these equations will assist practicing engineers to predict elastic behaivour of structures more precisely.