• Title/Summary/Keyword: Structural Changes

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Hypersensitive and Apoptotic Responses of Pepper Fruit Against Xnthomonas axonopodis pv. glycines Infection

  • Chang, Sung-Pae;Kim, Young-Ho
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.72.1-72
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    • 2003
  • Generally, plants defend themselves against pathogens by structural and biochemical reactions. Defense structures act as physical barriers and inhibit the pathogen from gaining entrance and spreading through the plant. Xanthomonas axonopodis pv glycines, the causal pathogen of bacterial pustule of soybean, causes hypersensitive response (HR). When pepper fruits were inoculated with X. axonopodis pv. glycines, in situ, time-series defense-related structural changes occurred in the inoculated sites. Early responses were programmed cell death (PCD), characterized by condensation and vacuolization of the cytoplasm, condensation of nuclear materials, and fragmentation of the nuclear DNA, which were observed by transmission electron microscopy. Nuclear fragmentation was proven by TUNEL method under confocal laser scanning microscopy and DNA laddering through eletrophoresis. At later stages, plant responses were cell elongation and cell division, forming a periderm-like boundary layer that demarcated healthy tissues from the inoculation sites. Using several stains such as toluidine blue, sudan IV, annexin V, and phloroglucinol-HCl, defense-related materials and structural changes were also examined.

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Microstructural Changes during Activation Process of Isotopic Carbon Fibers using CO2 Gas(I)-XRD Study (이산화탄소를 이용한 등방성 탄소섬유의 활성화과정 중 발생하는 구조변화(I)-XRD를 이용한 분석)

  • Roh, J.S.
    • Korean Journal of Materials Research
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    • v.13 no.11
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    • pp.742-748
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    • 2003
  • The structural parameters such as Lc, La and d of $CO_2$activated isotropic carbon fibers(ACFs) were obtained from XRD in order to understand a development mechanism of micropores. And the structural parameters were compared with specific surface area(SSA) data. The $d_{002}$, Lc, and La of the original fiber were measured to be 4.04$\AA$, 6.2$\AA$, and 23.6$\AA$, respectively. Carbonization of outer-parts and oxidization of inner-parts of the original fibers were far from completeness. It was observed that the structural changes of the ACFs during activation take place severely, therefore the carbonization and the oxidization of the fibers take place simultaneous with pore developments. The $d_{002}$ of the ACFs was increased to be 2.80$\AA$, and the La of the ACFs was decreased to be 17.0$\AA$ by activation. It was shown that the pores are developed continuously from the outer-parts to the inner-parts of the fibers, therefore the SSA increases as a result of the development of pores fully to the inner-parts of the fiber when the burn-off degree was over :39%. It seems that the (002) planes of crystallites contribute to the micropore wall related to the super high SSA.SSA.

Damage detection of mono-coupled multistory buildings: Numerical and experimental investigations

  • Xu, Y.L.;Zhu, Hongping;Chen, J.
    • Structural Engineering and Mechanics
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    • v.18 no.6
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    • pp.709-729
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    • 2004
  • This paper presents numerical and experimental investigations on damage detection of mono-coupled multistory buildings using natural frequency as only diagnostic parameter. Frequency equation of a mono-coupled multistory building is first derived using the transfer matrix method. Closed-form sensitivity equation is established to relate the relative change in the stiffness of each story to the relative changes in the natural frequencies of the building. Damage detection is then performed using the sensitivity equation with its special features and minimizing the norm of an objective function with an inequality constraint. Numerical and experimental investigations are finally conducted on a mono-coupled 3-story building model as an application of the proposed algorithm, in which the influence of modeling error on the degree of accuracy of damage detection is discussed. A mono-coupled 10-story building is further used to examine the capability of the proposed algorithm against measurement noise and incomplete measured natural frequencies. The results obtained demonstrate that changes in story stiffness can be satisfactorily detected, located, and quantified if all sensitive natural frequencies to damaged stories are available. The proposed damage detection algorithm is not sensitive to measurement noise and modeling error.

Structural health monitoring-based dynamic behavior evaluation of a long-span high-speed railway bridge

  • Mei, D.P.
    • Smart Structures and Systems
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    • v.20 no.2
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    • pp.197-205
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    • 2017
  • The dynamic performance of railway bridges under high-speed trains draws the attention of bridge engineers. The vibration issue for long-span bridges under high-speed trains is still not well understood due to lack of validations through structural health monitoring (SHM) data. This paper investigates the correlation between bridge acceleration and train speed based on structural dynamics theory and SHM system from three foci. Firstly, the calculated formula of acceleration response under a series of moving load is deduced for the situation that train length is near the length of the bridge span, the correlation between train speed and acceleration amplitude is analyzed. Secondly, the correlation scatterplots of the speed-acceleration is presented and discussed based on the transverse and vertical acceleration response data of Dashengguan Yangtze River Bridge SHM system. Thirdly, the warning indexes of the bridge performance for correlation scatterplots of speed-acceleration are established. The main conclusions are: (1) The resonance between trains and the bridge is unlikely to happen for long-span bridge, but a multimodal correlation curve between train speed and acceleration amplitude exists after the resonance speed; (2) Based on SHM data, multimodal correlation scatterplots of speed-acceleration exist and they have similar trends with the calculated formula; (3) An envelope line of polylines can be used as early warning indicators of the changes of bridge performance due to the changes of slope of envelope line and peak speed of amplitude. This work also gives several suggestions which lay a foundation for the better design, maintenance and long-term monitoring of a long-span high-speed bridge.

Differential Structural Responses of Ginseng Root Tissues to Different Initial Inoculum Levels of Paenibacillus polymyxa GBR-1

  • Jeon, Yong-Ho;Kim, Young-Ho
    • The Plant Pathology Journal
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    • v.24 no.3
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    • pp.352-356
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    • 2008
  • Root discs of 4-year-old ginseng, Panax ginseng C. A. Meyer, were inoculated with the higher($10^8$ colonyforming units(CFU)/ml) and lower($10^6\;or\;10^5$ CFU/ml) initial inoculum levels of a plant-growth promoting rhizobacterium(PGPR), Paenibacillus polymyxa GBR-1 to examine rot symptom development and bacterial population changes on the root discs. At the higher inoculum level, brown rot symptoms developed and expanded on the whole root discs in which the bacterial population increased continuously up to 4 days after inoculation. In light and electron microscopy, ginseng root cells on the inoculation sites were extensively decayed, which were characterized by dissolved cell walls and destructed cytoplasmic contents. However, no rot symptoms were developed and the bacterial population increased only during the initial two days of inoculation at the lower inoculum level($10^6$ CFU/ml) of P. polymyxa GBR-1. At the lower inoculum level($10^5$ CFU/ml), boundary layers with parallel periclinal cell divisions, structurally similar to wound periderm, were formed internal to the inoculation sites, beneath which the cells were intact containing numerous normal-looking starch granules and no disorganized cell organelles, suggesting that these structural features may be related to the suppression of symptom development, a histological defense mechanism.

FPSO Collision Analysis Using a Simplified Analytical Technique (간이 해석 기법을 이용한 FPSO 충돌 해석)

  • Han, Sang-Min;Ito, Hisashi
    • Journal of Ocean Engineering and Technology
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    • v.24 no.2
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    • pp.25-33
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    • 2010
  • Collision between vessels may lead to structural damage and penetration of hulls. The structural damage of a hull may eventually bring about global collapse of the hull girder and outflow of oil, which would contaminate seawater. Therefore, various regulations require the strength of a vessel after collision to satisfy given criteria, and owners usually request collision analyses to confirm the structural safety of their vessels. In the process of designing a vessel to satisfy the collision strength criteria, the strength has been assessed mostly by conducting collision analyses using numerical techniques, such as dynamic, non-linear, finite-element analysis. Design is an inherently iterative process during which many changes are necessary due to the endless needs for reinforcement and modification. Numerical techniques are not adequate for coping with a situation in which collision analysis is frequently required to provide the revised results that reflect the repetitive changes in designs. Numerical techniques require a lot of time and money to conduct in spite of recent improvements in computing power and in the productivity of modeling tools. Therefore, in this paper, an analytical technique is introduced and a collision problem is idealized and simplified using reasonable assumptions based on appropriate background. The technique was applied to an example of an actual FPSO and verified by comparing the results with results from the numerical technique. A good correlation was apparent between the results of the analytical and numerical techniques.

Vegetational Structure of Orchardgrass Sward II. Changes in dry matter production of the 2nd cutting by the times of established pasture (Orchargrass의 식생 구조 II. 조성년도가 다른 2번초의 건물생산성의 변화)

  • 이주삼;강치훈
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.9 no.1
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    • pp.15-19
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    • 1989
  • This experiment was carried out in order to know the changes in dry matter production of the 2nd cutting by the times of established pasture and its relation with morphological and structural characters of orchardgrass sward. Experimental pasture was established by the times of year, from 1 year to 7 year, 1986 through 1980, alternately. The results were summarizedas follows: 1. The population density of the 2nd cutting was extremely decreased by the times of established year. 2. The dry weight of $plantsW)$ was positively significant correlations with plant length(PL), number of tillers per plant (NT/pl.) and the dry weight of plant(DW/pl.). 3. The highest value of the dry weight of plants(DW) was obtained at 7 year old pasture. 4. The pasture productivity of 7 year old pasture was resulted by the increases of regrowth capacity, yield components and structural characters of the 2nd cutting. 5 . Relationship between morphological and structural characters concerned vegetational structure of the 2nd cutting were changeable year by year.

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A combined stochastic diffusion and mean-field model for grain growth

  • Zheng, Y.G.;Zhang, H.W.;Chen, Z.
    • Interaction and multiscale mechanics
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    • v.1 no.3
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    • pp.369-379
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    • 2008
  • A combined stochastic diffusion and mean-field model is developed for a systematic study of the grain growth in a pure single-phase polycrystalline material. A corresponding Fokker-Planck continuity equation is formulated, and the interplay/competition of stochastic and curvature-driven mechanisms is investigated. Finite difference results show that the stochastic diffusion coefficient has a strong effect on the growth of small grains in the early stage in both two-dimensional columnar and three-dimensional grain systems, and the corresponding growth exponents are ~0.33 and ~0.25, respectively. With the increase in grain size, the deterministic curvature-driven mechanism becomes dominant and the growth exponent is close to 0.5. The transition ranges between these two mechanisms are about 2-26 and 2-15 nm with boundary energy of 0.01-1 J $m^{-2}$ in two- and three-dimensional systems, respectively. The grain size distribution of a three-dimensional system changes dramatically with increasing time, while it changes a little in a two-dimensional system. The grain size distribution from the combined model is consistent with experimental data available.

Structural and Magnetic Properties of LiZnO Added MgFe2O4 Composite

  • Tadi, Ravindar;Kim, Yong-Il;Kim, Cheol-Gi;Ryu, Kwon-Sang
    • Journal of Magnetics
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    • v.15 no.4
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    • pp.165-168
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    • 2010
  • $Li_{0.1}Zn_{0.9}O$ and $MgFe_2O_4$ powders were synthesized using chemical methods and mixed in different proportions to prepare a mixture of $Li_{0.1}Zn_{0.9}O$ and $MgFe_2O_4$ that was thermally treated between 900 to $1100^{\circ}C$ for 1 hour. Structural characterization was done using X-ray powder diffraction measurements. Grain sizes and morphologies of $Li_{0.1}Zn_{0.9}O$, $MgFe_2O_4$, and $Li_{0.1}Zn_{0.9}O+MgFe_2O_4$ samples were observed using a scanning electron microscope. Variation of magnetic properties of the $Li_{0.1}Zn_{0.9}O+MgFe_2O_4$ samples due to the addition of $Li_{0.1}Zn_{0.9}O$ was studied in relation to the structural changes occurring due to the thermal treatment. In particular, changes in the cationic distribution between the tetrahedral and octahedral positions were studied with respect to the increase of the annealing temperature. Magnetization was found to be dependent on the cations distributed in the tetrahedral and octahedral sites of the $MgFe_2O_4$.

Effect of Bovine Serum Albumin on the Stability of Methotrexate-encapsulated Liposomes

  • Kim, Chong-Kook;Kim, Han-Sung;Lee, Beum-Jin;Han, Jeong-Hee
    • Archives of Pharmacal Research
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    • v.14 no.4
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    • pp.336-341
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    • 1991
  • The effect of bovine serum albumin (BSA) on the encapsulation efficiency and stability of liposomes containing methotrexate (MTX) having different surface charges and cholesterol contents were investigated. The encapsulation efficiency of MTX was lower and the release of MTX was faster by the addition of BSA. The leaking of MTX from lipid bilayer depends upon the BSA concentrations. These results may be derived from the interaction of BSA with lipid bilayers. The dynamic structural changes of BSA were monitored indirectly using circular dichroism spectra. Observed dynamic structural changes of BSA with liposomes are presumed to reflect the interaction of BSA with liposomes. Negatively charged liposomes have more strong interaction with BSA than neutral and positively charged liposomes. BSA attacks lipid bilayers whether it is at the inner or at the outer phase of lipid bilayer and induces leakage of entrapped MTX. Especially, negatively charged liposomes are more sensitive than others. The inclusion of cholesterol in the lipid layers inhibits the interaction of BSA with liposomes and shows protective effect against BSA-induced leakage of MTX. To endure the attacking of BSA liposomes as drug carriers should be made using cholesterol.

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