• Journal of Oriental Neuropsychiatry
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• v.18 no.3
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• pp.43-53
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• 2007

The purpose of this research is to investigate the connection between Stress Response Inventory(SRI), Happiness Index(HI), and Qi-training to find out the meaning of Qi-training on anti-stress. Method: The study group was consisted of 35 subjects training Qi and 179 subjects not training. We bad all subjects to reply to demographic questimnaire, SRI questimnaire and HI questimnaire. We made the Qi-training group write the kinds and periods of Qi seperately. Results and Conclussions : 1. The higer Happines Index score, the subjects bad lower Stress Response Iuventory total score. Each details were connected significantly. 2. The Qi-training group's mean SRI total scores and ill scores were higer than the non-training's significantly. 3. In Qi-training group, mean HI scores get higer as training longer.

• Molecules and Cells
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• v.39 no.2
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• pp.163-168
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• 2016

Caffeine has both positive and negative effects on physiological functions in a dose-dependent manner. C. elegans has been used as an animal model to investigate the effects of caffeine on development. Caffeine treatment at a high dose (30 mM) showed detrimental effects and caused early larval arrest. We performed a comparative proteomic analysis to investigate the mode of action of high-dose caffeine treatment in C. elegans and found that the stress response proteins, heat shock protein (HSP)-4 (endoplasmic reticulum [ER] chaperone), HSP-6 (mitochondrial chaperone), and HSP-16 (cytosolic chaperone), were induced and their expression was regulated at the transcriptional level. These findings suggest that high-dose caffeine intake causes a strong stress response and activates all three stress-response pathways in the worms, including the ER-, mitochondrial-, and cytosolic pathways. RNA interference of each hsp gene or in triple combination retarded growth. In addition, caffeine treatment stimulated a food-avoidance behavior (aversion phenotype), which was enhanced by RNAi depletion of the hsp-4 gene. Therefore, up-regulation of hsp genes after caffeine treatment appeared to be the major responses to alleviate stress and protect against developmental arrest.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.19 no.4
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• pp.45-51
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• 2020

In this study, a response spectrum analysis of an electric distributing board (EDB) was conducted to investigate seismic integrity in the design stage. For the seismic analysis, the required response spectra of a safe shutdown earthquake with 2% damping (RRS/SSE-2%) specified in GR-63-CORE Zone 4 was used as the ground spectral acceleration input. A finite element method modal analysis of the EDB was also performed to examine the occurrence of resonance within the frequency range of the earthquake response spectrum. Furthermore, static stress caused by deadweight was analyzed. The resultant total maximum stress of the EDB structure was calculated by adding the maximum stresses from both seismic and static loads using the square root of the sum of the squares (SRSS) method. Finally, the structural safety of the EDB was investigated by comparing the resultant total maximum stress with the allowable stress.

• Journal of Microbiology and Biotechnology
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• v.25 no.11
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• pp.1787-1795
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• 2015

The transition from primary to secondary metabolism in antibiotic-producing Streptomyces correlates with expression of genes involved in stress responses. Consequently, regulatory pathways that regulate specific stress responses are potential targets to manipulate to increase antibiotic titers. In this study, genes encoding key proteins involved in regulation of the osmotic stress response in Streptomyces avermitilis, the industrial producer of avermectins, are investigated as targets. Disruption of either osaB_Sa, encoding a response regulator protein, or osaC_Sa, encoding a multidomain regulator of the alternative sigma factor SigB, led to increased production of both oligomycin, by up to 200%, and avermectin, by up to 37%. The mutations also conditionally affected morphological development; under osmotic stress, the mutants were unable to erect an aerial mycelium. In addition, we demonstrate the delivery of DNA into a streptomycete using biolistics. The data reveal that information on stress regulatory responses can be integrated in rational strain improvement to improve yields of bioactive secondary metabolites.

• Mycobiology
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• v.42 no.2
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• pp.152-157
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• 2014

The iron uptake and utilization pathways play a critical role in allowing human pathogens, including Cryptococcus neoformans, the causative agent of fatal meningoencephalitis, to survive within the mammalian body by competing with the host for iron. Here we show that the iron regulon is also required for diverse environmental stress responses and that in C. neoformans, it is regulated by the high-osmolarity glycerol response (HOG) pathway. Between CFO1 and CFO2, two ferroxidase genes in the iron regulon, CFO1 but not CFO2 was induced during oxidative and osmotic stress. Interestingly, we found that the HOG pathway repressed basal expression of both CFO1 and CFO2. Furthermore, when the HOG pathway was blocked, CFO2 also responded to oxidative and osmotic stress and the response of CFO1 was increased. We also established that CFO1 plays a major role in responding and adapting to diverse environmental stresses, including oxidative and genotoxic damage, osmotic fluctuations, heavy metal stress, and stress induced by cell membrane destabilizers. Therefore, our findings indicate that in C. neoformans, the iron uptake and utilization pathways are not only required for iron acquisition and survival, but also play a significant role in the environmental stress response through crosstalk with the HOG pathway.

• Toxicological Research
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• v.28 no.1
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• pp.19-24
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• 2012

In the present study, toxicity of silver nanoparticles (AgNPs) was investigated in the nematode, Caenohabditis elegans focusing on the upstream signaling pathway responsible for regulating oxidative stress, such as mitogen-activated protein kinase (MAPK) cascades. Formation of reactive oxygen species (ROS) was observed in AgNPs exposed C.elegans, suggesting oxidative stress as an important mechanism in the toxicity of AgNPs towards C. elegans. Expression of genes in MAPK signaling pathways increased by AgNPs exposure in less than 2-fold compared to the control in wildtype C.elegans, however, those were increased dramatically in sod-3 (gk235) mutant after 48 h exposure of AgNPs (i.e. 4-fold for jnk-1 and mpk-2; 6-fold for nsy-1, sek-1, and pmk-1, and 10-fold for jkk-1). These results on the expression of oxidative stress response genes suggest that sod-3 gene expression appears to be dependent on p38 MAPK activation. The high expressions of the pmk-1 gene 48 h exposure to AgNPs in the sod-3 (gk235) mutant can also be interpreted as compensatory mechanisms in the absence of important stress response genes. Overall results suggest that MAPK-based integrated stress signaling network seems to be involved in defense to AgNPs exposure in C.elegans.

• Stress
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• v.27 no.2
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• pp.199-204
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• 2019

Backgoround: This study aimed to examine the influence of anxiety sensitivity on task performance and physiological stress response, and to assess the effect of depression in this process for the youth population. Methods: We presented participants with an uncontrollable stress situation where they were required to perform mental arithmetic, based on the Montreal Imaging Stress Task (MIST). A total of 29 participants volunteered for this study. They completed the Anxiety Sensitivity Index-Revised and Patient Health Questionnaire-9 to measure their levels of anxiety sensitivity and depression. Two saliva samples, one before and one after the experiment, were collected to assess the change in cortisol levels as an index of physiological stress response. Results: Participants with high anxiety sensitivity showed lower performance on the mental arithmetic tasks and a significant increase in a salivary cortisol level, compared to those with low anxiety sensitivity. Furthermore, cortisol levels showed a remarkable increase where high anxiety sensitivity was coupled with depressed mood. In other hands, the levels of cortisol remained unchanged despite high anxiety sensitivity with low depressed mood. Conclusions: Our results confirm that the interaction between anxiety sensitivity and depression affects participants' task performance and stress response, as measured through behavioral tasks and physiological data with self-report indices. Also, through the physiological data, we examined that those who have a high level of anxiety sensitivity showed maladaptive responses under high stressful situation.

• The Journal of Korean Obstetrics and Gynecology
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• v.18 no.1
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• pp.94-110
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• 2005

The purpose of this research was to investigate the effects of Kamikwibitang water extract (KKT) on immobilization stress in C57BL/6J mice. KKT decreased the serum level of histamine and corticosterone increased by immobilization stress. In addition, KKT decreased the cell viability of thymocytes and enhanced the cell viability of splenocytes decreased by immobilization stress. Also, KKT decreased the viability of thymocytes and splenocytes in vitro. KKT decreased DNA fragmentation of splenocytes increased by immobilization stress. KKT decreased the population of thymic $CD4^+CD8^-$ cells increased by immobilization stress, and did not affect the population of $B220^+$ cells and the population of $Thy1^+$ cells changed by immobilization stress and enhanced the population of splenic $CD4^-CD8^+$ cells increased by immobilization stress. KKT enhanced the production of ${\gamma}-interferon$ and did not affect the production of interleukin-2 and interleukin-4 decreased by immobilization stress. Also, KKT decreased the phagocytic activity and the level of nitric oxide decreased by immobilization stress. These results indicate that KKT may be useful for the prevention and treatment of stress via suppression of serum histamine and corticosterone level and enhancement of specific-immune response.

• Journal of Korean Clinical Health Science
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• v.8 no.2
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• pp.1417-1425
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• 2020

Purpose: The purpose of this study was to check the stress, stress and coping ability of nursing college students and to identify factors that influence college life adjustment. Method: This study was a descriptive research study to confirm the correlation between stress, stress response, coping ability, and college life adaptation of nursing college students, and to analyze the factors that influence college life adjustment. The data collection period was from August 17 to 23, 2020, for one week, the researcher directly explains the purpose and purpose of the study to 221 nursing college students nationwide, and online surveys to those who have agreed to participate in the study. The collected data were analyzed using SPSS Win 20.0 program. Results: Adaptation to college was negatively correlated with stress (r=-.285, p<.001), stress response (r=-.258, p<.001), and coping ability (r=.512, p<.001) had a positive correlation. The most significant factor influencing the study subjects' adaptation to college life was coping ability (β=.473, p<.001), followed by stress (β=-.185, p=.002) in the order of The explanatory power of the factors affecting the subject's adaptation to college life was 28.9%. Conclusion: As a result of this study, it means that nursing students' adaptation to college life has less stress and stress response, and higher coping ability increases college life adaptation. The factor that influenced the adjustment to college life was the most significant factor, followed by stress. In order for nursing students to adjust to college life smoothly, it is thought that coping measures to reduce stress and stress responses and overcome them are necessary.

• Korean Journal of Psychosomatic Medicine
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• v.12 no.1
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• pp.47-55
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• 2004

Objectives: The aim of this study was to investigate alexithymia, depressive symptoms, and stress response of patients with alcohol dependence. The results were taken as a basic data of ameliorating the quality of life. Methods: 94 patients with alcohol dependence completed stress response inventory, Korea depression scale, and toronto alexithymia scale successfully. Results: 55% of variance of alexithymia of patients with alcohol dependence were explained by the stress response and depressive symptoms and score of ADS, and the stress response had the most explanatory power. Conclusions: The alexithymia of patients with alcohol dependence may be influenced by depressive symptoms, education, frequency of drinking, and stress response. The specialists were identifying symptoms of depression and optimizing the management, therefore increasing the compliance and quality of life of patients with alcohol dependence.