• 동의신경정신과학회지
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• 제18권3호
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• pp.43-53
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• 2007

The purpose of this research is to investigate the connection between Stress Response Inventory(SRI), Happiness Index(HI), and Qi-training to find out the meaning of Qi-training on anti-stress. Method: The study group was consisted of 35 subjects training Qi and 179 subjects not training. We bad all subjects to reply to demographic questimnaire, SRI questimnaire and HI questimnaire. We made the Qi-training group write the kinds and periods of Qi seperately. Results and Conclussions : 1. The higer Happines Index score, the subjects bad lower Stress Response Iuventory total score. Each details were connected significantly. 2. The Qi-training group's mean SRI total scores and ill scores were higer than the non-training's significantly. 3. In Qi-training group, mean HI scores get higer as training longer.

• Molecules and Cells
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• 제39권2호
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• pp.163-168
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• 2016

Caffeine has both positive and negative effects on physiological functions in a dose-dependent manner. C. elegans has been used as an animal model to investigate the effects of caffeine on development. Caffeine treatment at a high dose (30 mM) showed detrimental effects and caused early larval arrest. We performed a comparative proteomic analysis to investigate the mode of action of high-dose caffeine treatment in C. elegans and found that the stress response proteins, heat shock protein (HSP)-4 (endoplasmic reticulum [ER] chaperone), HSP-6 (mitochondrial chaperone), and HSP-16 (cytosolic chaperone), were induced and their expression was regulated at the transcriptional level. These findings suggest that high-dose caffeine intake causes a strong stress response and activates all three stress-response pathways in the worms, including the ER-, mitochondrial-, and cytosolic pathways. RNA interference of each hsp gene or in triple combination retarded growth. In addition, caffeine treatment stimulated a food-avoidance behavior (aversion phenotype), which was enhanced by RNAi depletion of the hsp-4 gene. Therefore, up-regulation of hsp genes after caffeine treatment appeared to be the major responses to alleviate stress and protect against developmental arrest.

• 한국기계가공학회지
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• 제19권4호
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• pp.45-51
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• 2020

In this study, a response spectrum analysis of an electric distributing board (EDB) was conducted to investigate seismic integrity in the design stage. For the seismic analysis, the required response spectra of a safe shutdown earthquake with 2% damping (RRS/SSE-2%) specified in GR-63-CORE Zone 4 was used as the ground spectral acceleration input. A finite element method modal analysis of the EDB was also performed to examine the occurrence of resonance within the frequency range of the earthquake response spectrum. Furthermore, static stress caused by deadweight was analyzed. The resultant total maximum stress of the EDB structure was calculated by adding the maximum stresses from both seismic and static loads using the square root of the sum of the squares (SRSS) method. Finally, the structural safety of the EDB was investigated by comparing the resultant total maximum stress with the allowable stress.

• Journal of Microbiology and Biotechnology
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• 제25권11호
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• pp.1787-1795
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• 2015

The transition from primary to secondary metabolism in antibiotic-producing Streptomyces correlates with expression of genes involved in stress responses. Consequently, regulatory pathways that regulate specific stress responses are potential targets to manipulate to increase antibiotic titers. In this study, genes encoding key proteins involved in regulation of the osmotic stress response in Streptomyces avermitilis, the industrial producer of avermectins, are investigated as targets. Disruption of either osaB_Sa, encoding a response regulator protein, or osaC_Sa, encoding a multidomain regulator of the alternative sigma factor SigB, led to increased production of both oligomycin, by up to 200%, and avermectin, by up to 37%. The mutations also conditionally affected morphological development; under osmotic stress, the mutants were unable to erect an aerial mycelium. In addition, we demonstrate the delivery of DNA into a streptomycete using biolistics. The data reveal that information on stress regulatory responses can be integrated in rational strain improvement to improve yields of bioactive secondary metabolites.

• Mycobiology
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• 제42권2호
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• pp.152-157
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• 2014

The iron uptake and utilization pathways play a critical role in allowing human pathogens, including Cryptococcus neoformans, the causative agent of fatal meningoencephalitis, to survive within the mammalian body by competing with the host for iron. Here we show that the iron regulon is also required for diverse environmental stress responses and that in C. neoformans, it is regulated by the high-osmolarity glycerol response (HOG) pathway. Between CFO1 and CFO2, two ferroxidase genes in the iron regulon, CFO1 but not CFO2 was induced during oxidative and osmotic stress. Interestingly, we found that the HOG pathway repressed basal expression of both CFO1 and CFO2. Furthermore, when the HOG pathway was blocked, CFO2 also responded to oxidative and osmotic stress and the response of CFO1 was increased. We also established that CFO1 plays a major role in responding and adapting to diverse environmental stresses, including oxidative and genotoxic damage, osmotic fluctuations, heavy metal stress, and stress induced by cell membrane destabilizers. Therefore, our findings indicate that in C. neoformans, the iron uptake and utilization pathways are not only required for iron acquisition and survival, but also play a significant role in the environmental stress response through crosstalk with the HOG pathway.

• Toxicological Research
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• 제28권1호
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• pp.19-24
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• 2012

In the present study, toxicity of silver nanoparticles (AgNPs) was investigated in the nematode, Caenohabditis elegans focusing on the upstream signaling pathway responsible for regulating oxidative stress, such as mitogen-activated protein kinase (MAPK) cascades. Formation of reactive oxygen species (ROS) was observed in AgNPs exposed C.elegans, suggesting oxidative stress as an important mechanism in the toxicity of AgNPs towards C. elegans. Expression of genes in MAPK signaling pathways increased by AgNPs exposure in less than 2-fold compared to the control in wildtype C.elegans, however, those were increased dramatically in sod-3 (gk235) mutant after 48 h exposure of AgNPs (i.e. 4-fold for jnk-1 and mpk-2; 6-fold for nsy-1, sek-1, and pmk-1, and 10-fold for jkk-1). These results on the expression of oxidative stress response genes suggest that sod-3 gene expression appears to be dependent on p38 MAPK activation. The high expressions of the pmk-1 gene 48 h exposure to AgNPs in the sod-3 (gk235) mutant can also be interpreted as compensatory mechanisms in the absence of important stress response genes. Overall results suggest that MAPK-based integrated stress signaling network seems to be involved in defense to AgNPs exposure in C.elegans.

• 스트레스연구
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• 제27권2호
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• pp.199-204
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• 2019

본 연구는 불안민감도가 과제수행과 스트레스 반응에 미치는 영향을 살펴보고, 이 과정에 우울감은 어떠한 영향을 미치는지 확인하였다. 참가자들은 제한된 시간에 암산 문제를 완수하였고, 불안민감도지수와 우울증 선별도구에 응답하였다. 더불어, 실험 전후 참가자들의 코르티졸(Cortisol) 변화량을 확인하였다. 그 결과, 불안민감도가 높은 사람은 과제수행의 정확도가 떨어지고 코르티졸 수치가 유의미하게 상승하였다. 또한, 불안민감도와 우울감이 모두 높은 참가자들이 코르티졸 수치가 가장 크게 상승하였다. 본 연구는 자기보고식 응답에 근거하여 임상증상을 기술하는 것을 넘어 과제수행의 정확도와 생화학적 지표를 함께 제시함으로써, 보다 안정적으로 개인의 불안민감도와 우울감의 관계를 논의하였다는 의의를 지닌다.

• 대한한방부인과학회지
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• 제18권1호
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• pp.94-110
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• 2005

The purpose of this research was to investigate the effects of Kamikwibitang water extract (KKT) on immobilization stress in C57BL/6J mice. KKT decreased the serum level of histamine and corticosterone increased by immobilization stress. In addition, KKT decreased the cell viability of thymocytes and enhanced the cell viability of splenocytes decreased by immobilization stress. Also, KKT decreased the viability of thymocytes and splenocytes in vitro. KKT decreased DNA fragmentation of splenocytes increased by immobilization stress. KKT decreased the population of thymic $CD4^+CD8^-$ cells increased by immobilization stress, and did not affect the population of $B220^+$ cells and the population of $Thy1^+$ cells changed by immobilization stress and enhanced the population of splenic $CD4^-CD8^+$ cells increased by immobilization stress. KKT enhanced the production of ${\gamma}-interferon$ and did not affect the production of interleukin-2 and interleukin-4 decreased by immobilization stress. Also, KKT decreased the phagocytic activity and the level of nitric oxide decreased by immobilization stress. These results indicate that KKT may be useful for the prevention and treatment of stress via suppression of serum histamine and corticosterone level and enhancement of specific-immune response.

• 한국임상보건과학회지
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• 제8권2호
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• pp.1417-1425
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• 2020

Purpose: The purpose of this study was to check the stress, stress and coping ability of nursing college students and to identify factors that influence college life adjustment. Method: This study was a descriptive research study to confirm the correlation between stress, stress response, coping ability, and college life adaptation of nursing college students, and to analyze the factors that influence college life adjustment. The data collection period was from August 17 to 23, 2020, for one week, the researcher directly explains the purpose and purpose of the study to 221 nursing college students nationwide, and online surveys to those who have agreed to participate in the study. The collected data were analyzed using SPSS Win 20.0 program. Results: Adaptation to college was negatively correlated with stress (r=-.285, p<.001), stress response (r=-.258, p<.001), and coping ability (r=.512, p<.001) had a positive correlation. The most significant factor influencing the study subjects' adaptation to college life was coping ability (β=.473, p<.001), followed by stress (β=-.185, p=.002) in the order of The explanatory power of the factors affecting the subject's adaptation to college life was 28.9%. Conclusion: As a result of this study, it means that nursing students' adaptation to college life has less stress and stress response, and higher coping ability increases college life adaptation. The factor that influenced the adjustment to college life was the most significant factor, followed by stress. In order for nursing students to adjust to college life smoothly, it is thought that coping measures to reduce stress and stress responses and overcome them are necessary.

• 정신신체의학
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• 제12권1호
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• pp.47-55
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• 2004

연구목적: 알콜 의존 환자의 우울 증상과 스트레스에 대한 반응과 감정표현불능증에 대하여 알아본 후, 상관관계에 대하여 조사하여 향후 환자의 삶의 질을 높일 수 있는 치료적 중재를 위한 기초 자료로 사용하고자 본 연구를 시행하였다. 방 법: 알콜 의존으로 진단을 받은 환자 94명과 정신과 치료 경험이 없는 사람 34명을 대상으로 하여 한국우울증척도와 스트레스 반응 척도와 감정표현불능증 척도를 시행하였다. 결 과: 우울 증상과 스트레스 반응 요인과 알콜 의존 지수가 알콜 의존 환자의 감정표현불능증 전체 변이 중에 약 55%를 설명하였으며, 이 중 스트레스에 대한 반응이 가장 큰 설명력을 보였다. 결 론: 우울 증상, 스트레스에 대한 반응, 학력, 음주 회수 등이 알콜 의존 환자의 감정표현불능증과 가장 밀접한 관련이 있는 요인으로 밝혀졌으며, 환자가 경험하는 신체화 증상을 포함한 우울 증상과 스트레스에 대한 정신과 의사의 관심이 필요함을 시사하는 것으로, 알콜 의존 환자의 우울 증상과 감정표현불능증에 대한 적극적 치료를 통하여 궁극적으로 환자의 삶의 질을 높일 수 있도록 하는 정신과 의사의 노력이 필요하리라 본다.