• 한국미생물·생명공학회지
• /
• 제35권4호
• /
• pp.292-297
• /
• 2007

H. pluviails는 고광도와 질소 결핍 배지 조건에서 ketocarotenoid의 일종인 astaxanthin을 다량 축적하는 녹조류이다. 스트레스가 없는 조건에서 키운 green cell과 astaxanthin이 합성된 red cell을 HPLC를 통해 비교해 본 결과 각 색소의 양이 변화하는 것을 볼 수 있었다. 여러 ester 형태의 astaxanthin이 생합성 되고, zeaxanthin이 늘어난 반면, lutein과 ${\beta}$-carotene은 감소하였다. 또한 total chlorophyll 양이 줄어드는 대신 total carotenoid의 양이 늘어남을 보였다. H. pluvilalis에서 찾아낸 astaxanthin 생합성 경로에 있는 carotenoid hydroxylase, phytoene desaturase, isopentenyl pyrophosphate isomerase, ${\beta}$-carotene ketolase 유전자는 음성대조군인 chloroplast chlorophyll a-b binding protein와는 달리cell이 성장하기 좋은 조건의 상태보다 astaxanthin을 생합성하기 위해 고광도의 스트레스를 받았을 때 더 높은 발현양상을 보이는 것을 확인할 수 있었다.

• IMMUNE NETWORK
• /
• 제5권4호
• /
• pp.232-236
• /
• 2005

Background: A physically active lifestyle and regular exercise training incurs many health benefits. One recently recognized benefit of regular moderate exercise is stress reduction and immune enhancement. Thus, a physical stress such as exercise may act at any number of points in the complex sequence of events collectively termed the immune response. Although exercise causes many propound changes in parameters of immune function, the nature and magnitude of such changes rely on several factors including the immune parameters of interest; type, intensity, and duration of exercise; fitness level or exercise history of the subject; environmental factors such as ambient temperature and humidity. Methods: This study was undertaken to investigate the effect of different type of exercise on superoxide dismutase (SOD), neutrophils, and T lymphocytes of Sprague-Dawley rats. Sprague-Dawley rats were randomly divided into three groups; a non-Trained group (NTG, n=6), a swim-Trained group (STG), and a treadmill-Trained group (TTG). The exercise regimen was designed in a treadmill (5 times/5 days/week) during 8-weeks for TTG, and swim training (5 times/5 days/week) during 8-weeks for STG, and the volume of exercise training was the same in both groups. Results: 8 weeks of regular swim and treadmill training significantly increased liver SOD concentration however, muscle SOD concentration was not statistically significant. In the level of neutrophils, TTG and STG showed significant difference, compared to NTG. TTG was the highest level of neutrophils. In the level of immune cell counts, there was significant difference among TTG, STG, and NTG both in the spleen and thymus. Conculsion: In conclusion, it can be stated that eight weeks swim and treadmill exercise training has beneficial effect in improving immune response and antioxidant defence capacity by augmenting immune cells and SOD activities of SD rats.

• 한국기계가공학회지
• /
• 제13권1호
• /
• pp.92-99
• /
• 2014

An aspherical lens, which resolves several problems with a spherical lens,typically serves asa key part of an optical system. Generally, an aspherical lens is fabricated using a diamond turning machine or by mean of injection molding. However, residual stress and/or tool marks can arise when using a commercial fabricating method such as DTM or injection molding. A polishing process, thus, is commonly used to obtain a high-precision aspherical lens. In this study, a polishing method using MR fluid was applied to minimize several problems, in this case residual stress and the creation of tool marks, during the cutting process. The MR polishing system was developed to polish aspherical lenses. A series of experiments were performed to obtain a very fine surface roughness. PMMA (the lens material for molding) was used as a workpiece, and the gap size, magnetic field intensity, wheel speed and feed rate were selected as the parameters in this study. Finally, a very fine surface roughness of Ra=2.12nm was obtained after MR polishing.

• 대한한의학회지
• /
• 제39권1호
• /
• pp.35-43
• /
• 2018

Objectives: In the brain, glutamate is the most important excitable neurotransmitter in physiological and pathological conditions. However, the high level of glutamate induces neuronal cell death due to exitotoxicity and oxidative stress. The present study investigated to evaluate a possible neuroprotective effect of furosin isolated from Euphorbia helioscopia against glutamate-induced HT22 cell death. Methods: Furosin was isolated from methanol extract of Euphorbia helioscopia and examined whether it protects glutamate-induced neuronal cell death. The cell viability was determined using Ez-Cytox assay. Anti-oxidative effect of furosin was determined by DPPH scavenging activities, and the levels of intracellular reactive oxygen species (ROS) were determined by the fluorescent intensity after staining the cells with $H_2DCFDA$. To evaluate apoptotic cell death, we performed nuclear staining and image-based cytometeric analysis. Results: The cell viability was significantly increased by treatement with furosin compared with the treatment with glutamate. Furosin showed a strong DPPH radical scavenging activity ($EC50=1.83{\mu}M$) and prevented the accumulation of intra cellular ROS. Finally, the presence of 50 and $100{\mu}M$ furosin significantly the percentage of apoptotic cells compared with glutamate treatment. Conclusion: The present study found that furosin is a potent neuroprotectant against glutamate-induced oxidative stress through inhibition of apoptotic cell death induced by glutamate. Therefore, the present study suggests that furosin as a bioactive compound of E. helioscopia can be a useful source to develop a drug for the treatment of neurodegenerative diseases and acute brain injuries.

• Journal of Mechanical Science and Technology
• /
• 제19권2호
• /
• pp.634-648
• /
• 2005

Performed here is a comparative assessment study for the probabilistic fracture mechanics approach of the pressurized thermal shock of the reactor pressure vessel. A round robin consisting of one prerequisite deterministic study and five cases for probabilistic approaches is proposed, and all organizations interested are invited. The problems are solved by the participants and their results are compared to issue some recommendation of best practices and to assure an understanding of the key parameters in this type of approach, like transient description and frequency, material properties, defect type and distribution, fracture mechanics methodology etc., which will be useful in the justification through a probabilistic approach for the case of a plant over-passing the screening criteria. Six participants from 3 organizations responded to the problem and their results are compiled and analyzed in this study.

• 수산해양교육연구
• /
• 제9권2호
• /
• pp.198-208
• /
• 1997

Recently, with rapid increase of gas demand, there occurs much interest their security of safety in the gas storage tanks and pressure vessels etc. In order to solve the problems, the occurrence of corrosion fatigue crack and the propagation behavior must be investigated. Especially the occurrence of corrosion fatigue crack and the propagation behavior in the part which has concentrated stress or defects, must be studied more carefully. In this paper, the high-tensile steel of SPV 50 which is much used for building the LPG storage tanks was tested by the use of a plane bending corrosion fatigue tester under the various marine environment and in the air. These experiments were carried out to investigate the surface crack propagation behavior, the value of experimental constant for Paris' rule(da/dN=$C(K)^m$), the crack depth propagation rate and the accelerative factor of the surface crack propagation rate. The main results obtained are as follows ; 1) As the specific resistances of marine environment decreases, the exponential value of slope m of Paris' rule(da/dN=$C(K)^m$) decreases and the value of intercept C increases. 2) The surface crack propagation rate and the crack depth propagation rate are delayed, as the specific resistances of marine environment is increased. 3) The accelerative factor of the surface crack propagation rate by corrosion fatigue is higher, according as the stress intensity factor range ${\Delta}K_A$ is small.

• 자원환경지질
• /
• 제13권1호
• /
• pp.1-19
• /
• 1980

본논문(本論文)에서 1926년 2월부터 1943년 5월까지 국내(國內)에서 Wiechert 지진계(地震計)로 관측(觀測)된 국지자료(局地資料)가 제시(提示) 연구되었다. 일본(日本) 기상청(氣象廳)(JMA) 현용(現用) S-P monogram(travel time table)을 기초(基礎)로 하고 주로 Tsuboi의 지진(地震) 규모(規模)(magnitude) 계산식(式)과 진도자료(震度資料)의 보조(補助) 이용(利用)으로 적절한 한계내(限界內)에서 가능한 한(限) 많은 지진요소(地震要素)(parameter)를 산출하였다. 또한 진앙분포(震央分布)와 관련한 지진(地震) 특성(特性)이 인접지질구조(隣接地質構造)와 연관(連關) 논의(論議)되었으며 몇몇의 통계결과(統計結果)가 일본(日本) 구주지역(九州地域)과 비교 분석됨으로서 한국의 지진(地震) 활동(活動)에 관한 합리적(合理的)인 해석(解析)이 내려졌다. 지진(地震) mechanisrn을 규명(規明)하기에는 충분(充分)하지 않지만, 단편적인 자료(資料)들을 superposition method 에 의하여 종합(綜合)한 결과(結果), 일본(日本) 남서부(南西部)(구주(九州)) 지역(地域)의 그것과 대체로 일치(一致)하는 동일서(東一西) 압축(壓縮)의 stress field가 작용(作用)하는 일반적 경향성(傾向性)을 발견(發見)할 수 있었다.

• 환경생물
• /
• 제22권1호
• /
• pp.233-241
• /
• 2004

겨울철 저온에 의한 식물의 반응 및 생리적 상태를 확인하기 위해 문주란(Crinum asiaticum var. japonicum)을 대상으로 엽록소형광과 항산화효소 찬성의 변화를 조사하였다. O-J-I-P곡선을 분석한 결과 겨울철에 J, I, P-단계에서의 형광세기가 현저하게 간소하였다. 문주란 잎의 광계II의 광화학적 효율, 즉 Fv/Fm은 겨울철의 온도변화 추이와 유사하게 낮은 값을 나타내어 겨울철 저온이 스트레스 요인으로. 작용하는 것으로 보인다. 그리고, 겨울철에 superoxide dismutase, ascorbate peroxidase, peroxidase 활성 이 다소 증가하였으나 catalase 활성은 여름철에 비하여 크게 낮았다. 이러한 결과는 겨울철의 Fv/Fm의 감소와 함께 문주란이 겨울철 저온에 의해 산화적 스트레스에 처해 있게되며 세포사멸이 일어나는 것으로 해석된다.

• 한국지반공학회논문집
• /
• 제29권1호
• /
• pp.13-22
• /
• 2013

본 논문에서는 계절적 환경변화로 인한 습윤-건조 반복과정에 노출되는 옹벽의 시간 의존적 거동에 대한 수치 해석 연구 내용을 다루었다. 이를 위해 먼저 습윤-건조 과정에 노출되는 옹벽에 대한 응력-간극수압 연계해석 기반의 모델링 기법을 정립하였으며 이러한 모델링 기법을 토대로 다양한 옹벽조건 및 강우조건에 대한 매개변수 연구를 수행하였다. 그 결과 다양한 강우 특성 중 옹벽의 거동에 미치는 주된 영향인자는 강우강도로 나타났다. 한편, 강우가 발생하기 이전 선행하는 선행강우는 지반내 포화도, 즉 모관흡수력, 분포를 좌우하는 것으로 나타나 지반구조물의 안정성은 선행강우량에 의해 큰 영향을 받는 것으로 검토 되어 강우로 인한 습윤-건조 반복 사이클에 노출되는 옹벽구조물의 거동 예측시 선행강우량에 대한 현실적인 평가가 매우 중요한 것으로 검토되었다.

• 대한기계학회논문집A
• /
• 제39권6호
• /
• pp.583-588
• /
• 2015

완전접촉 문제를 이론적으로 해석하기 위해서 점근해법이 많이 사용된다. 점근해로서의 응력장은 특이항 만으로 구성되므로 접촉경계로부터 멀어질수록 정확도가 감소한다. 이에 반해 유한요소해석 방법은 요소크기의 제한으로 인해 완전접촉 문제에서의 응력특이성을 엄밀히 표현할 수 없다. 따라서 본 연구에서는 이론적 해법을 보조하고 또 그와 비교하기 위해 응착접촉 상태에 있는 완전접촉 문제를 이론적으로 해석한 후, 모아레 실험 및 유한요소해석 방법으로 접촉부 부근의 응력장을 분석하였다. 실험은 알루미늄과 구리 합금을 접촉각 $120^{\circ}$, $135^{\circ}C$로 가공하여 수행하였으며 모아레 무늬로부터 얻은 변위장과 유한요소해석을 수행한 결과와 비교하였다. 이로부터 타당성이 확보된 수치적 방법을 이용하여 실험조건에서의 일반화 응력확대계수와 접촉부 응력장을 구하여 이론 해와 비교하였으며, 접촉경계로부터 멀어질 때 나타나는 이론과 수치 해의 차이를 분석하였다.