• The Korean Journal of Mycology
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• v.38 no.2
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• pp.179-183
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• 2010

This study is to characterize the postulated anti-inflammatory effect of the hot water extracts from the Phellinus baumii. RAW264.7, macrophage cell line, was activated by lipopolysaccharide (LPS) and, further, treated with Phellinus baumii's aqueous extract. When the cultured macrophage cells were treated with LPS, they show typical signs of endoplasmic reticulum stress (ERS) and an increment in secretion of inflammatory cytokine compared to the non-treated control: The expression of glucose-regulated protein78 (Grp78), Grp94, and C/EBP homologous protein/GADD 153 (CHOP) increased along with augmented secretion of interlukin-6. Cellular nitric oxide content also significantly went up in comparison to the non-LPS treatment. When the LPS-treated RAW264.7 was treated with the aqueous Phellinus baumii extracts, however, the expression of ERS markers markedly reduced and the release of nitric oxide declined. Also, the expression of induced nitric oxide synthase (iNOS) notably diminished similarly as the NO content. In conclusion, this study strongly indicated that aqueous Phellinus baumii extract can be utilized directly as anti-inflammation agent and serves as a source of functional ingredient to lessen the inflammation.

• Tunnel and Underground Space
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• v.21 no.5
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• pp.394-405
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• 2011

In this paper, we applied coupled non-isothermal, multiphase fluid flow and geomechanical numerical modeling using TOUGH-FLAC coupled analysis to study the complex thermodynamic and geomechanical performance of underground lined rock caverns (LRC) for compressed air energy storage (CAES). Mechanical stress in concrete linings as well as pressure and temperature within a storage cavern were examined during initial and long-term operation of the storage cavern for CAES. Our geomechanical analysis showed that effective stresses could decrease due to air penetration pressure, and tangential tensile stress could develop in the linings as a result of the air pressure exerted on the inner surface of the lining, which would result in tensile fracturing. According to the simulation in which the tensile tangential stresses resulted in radial cracks, increment of linings' permeability and air leakage though the linings, tensile fracturing occurred at the top and at the side wall of the cavern, and the permeability could increase to $5.0{\times}10^{-13}m^2$ from initially prescribed $10{\times}10^{-20}m^2$. However, this air leakage was minor (about 0.02% of the daily air injection rate) and did not significantly impact the overall storage pressure that was kept constant thanks to sufficiently air tight surrounding rocks, which supports the validity of the concrete-lined underground caverns for CAES.

• Tunnel and Underground Space
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• v.31 no.1
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• pp.41-51
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• 2021

The hydraulic fracturing developed to improve permeability of tight reservoir is one of key stimulation technologies for developing unconventional resources such as shale gas and deep geothermal energy. The experimental study was conducted to improve disadvantage of hydraulic fracturing which has simple fracture pattern and poor fracturing efficiency. The fracturing experiments was conducted for tight rock using various fracturing fluids, water, N2, and CO2 and the created fracture pattern and fracturing efficiency was analyzed depending on fracturing fluids. The borehole pressure increased rapidly and then made fractures for hydraulic fracturing with constant injection rate, however, gas fracturing shows slowly increased pressure and less fracture pressure. The 3D tomography technic was used to generate images of induced fracture using hydraulic and gas fracturing. The stimulated reservoir volume (SRV) was estimated increment of 5.71% (water), 12.72% (N2), and 43.82% (CO2) respectively compared to initial pore volume. In addition, permeability measurement was carried out before and after fracturing experiments and the enhanced permeability by gas fracturing showed higher than hydraulic fracturing. The fracture conductivity was measured by increasing confining stress to consider newly creating fracture and closing induced fracture right after fracturing. When the confining stress was increased from 2MPa to 10MPa, the initial permeability was decreased by 89% (N2) and 50% (CO2) respectively. This study shows that the gas fracturing makes more permeability enhancement and less reduction of induced fracture conductivity than hydraulic fracturing.

• Korean Journal of Medicinal Crop Science
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• v.14 no.4
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• pp.206-211
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• 2006

This study was performed to examine antitumor activities of Rubus coreanus Miquel and Angelica gigas Nakai extracts against sarcoma-180 and anti-stress activities in ICR mice. The variation of body weights of the 20 days of Rubus coreanus extracts-administrated mice group was very low. The survival rate (T/C %) of Rubus coreanus extract administrated group was 161% after 50 days from the inoculation of sarcoma-180 and the increment of their body weights was suppressed. Anti-stress effect of the extracts of R. coreanus and A. gigas were estimated by maeasuring blood chemical value and internal organs weight in ICR mice. The extracts of R. coreanus reduced the cholesterol and glucose to the normal level in the all stress animal models. The extracts of R. coreanus reduced the hypertrophy of the internal organs such as adrenal, spleen and liver to the regular level.

• Journal of Applied Biological Chemistry
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• v.59 no.4
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• pp.305-311
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• 2016

This study investigated the effects of a mixture of fenugreek seeds and Lespedeza cuneata extracts on testosterone synthesis in TM3 cells that were oxidatively stressed with $H_2O_2$. In order to oxidatively stress TM3 cells, the cells were treated with $50{\mu}M$ hydrogen peroxide for 4 hr in serum-free media. Yagwanmun-horopa mixture (YHM) showed neither cytotoxicity nor increment of cell proliferation in the oxidatively stressed TM3 cells in any concentration. When the cells were treated with hydrogen peroxide, testosterone levels decreased, but the testosterone level was returned to that of the control level in the presence of YHM. In order to find out the reasons for the increase of testosterone, the expression of the genes involved in the synthesis or disintegration of testosterone. On the other hand, the levels of $3{\beta}$-HSD4 and 17, 20-desmorase, which are involved in testosterone synthesis, were decreased through the use of hydrogen peroxide and were recovered through YHM treatment. Aromatase and $5{\alpha}$-reductase2, which convert testosterone to estradiol and dihydrotestosterone, respectively, were increased through the use of hydrogen peroxide, and were returned to control level through YHM treatment. These results suggest that YHM does not affect TM3 cell proliferation. However, YHM increases the expression of testosterone-synthesizing enzyme, which was decreased through oxidative stress, and decreases the expression of testosterone- converting enzyme, which was increased through oxidative stress. Therefore, it is reasonable that YHM has strong recovery activity on testosterone to normal level, even in the oxidatively stressed TM3 cells which mimics the andropause state.

• Journal of the Korean Geotechnical Society
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• v.23 no.9
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• pp.5-16
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• 2007

The simple linear elastic-perfectly plastic model with soil parameters $s_u,\;E_u$ and n of undrained condition is usually applied to predict the displacement of a constructed diaphragm wall(DW) on soft soils during excavation. However, the application of this soil model for finite element analysis could not interpret the continued increment of the lateral displacement of the DW for the large and deep excavation area both during the elapsed time without activity of excavation and after finishing excavation. To study the characteristic behaviors of soil behind the DW during the periods without excavation, a series of tests on soft Bangkok clay samples are simulated in the same manner as stress condition of soil elements happening behind diaphragm wall by triaxial tests. Three kinds of triaxial tests are carried out in this research: $K_0$ consolidated undrained compression($CK_0U_C$) and $K_0$ consolidated drained/undrained unloading compression with periodic decrement of horizontal pressure($CK_0DUC$ and $CK_0UUC$). The study shows that the shear strength of series $CK_0DUC$ tests is equal to the residual strength of $CK_0UC$ tests. The Young's modulus determined at each decrement step of the horizontal pressure of soil specimen on $CK_0DUC$ tests decreases with increase in the deviator stress. In addition, the slope of Critical State Line of both $CK_0UC$ and $CK_0DUC$ tests is equal. Moreover, the axial and radial strain rates of each decrement of horizontal pressure step of $CK_0DUC$ tests are established with the function of time, a slope of critical state line and a ratio of deviator and mean effective stress. This study shows that the results of the unloading compression triaxial tests can be used to predict the diaphragm wall deflection during excavation.

• Korean Journal of Soil Science and Fertilizer
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• v.7 no.1
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• pp.35-42
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• 1974

From the results of countrywide two year N. P. K simple trial with newly bred IR 667-Suwon 214 and leading local varieties for comparison soil productivity (yield without fertilizer) and fertilization productivity (yield increase with fertilizer) were investigated. 1. IR667 showed higher yield than leading local by 19% in 1971 and by 12% in the year of low temperature stress, 1972. High yielding capacity of IR667 was attributed to greater number of spikelet per panicle. The coefficient of variation in yield was smaller in IR667 but the leading locals showed higher yield in 23(1971) and 30% (1972) of the fields. 2. IR667 required more nitrogen, especially phosphorus and potassium. Fertilization efficiency (yield increment per unit fertilizer) in IR667 was-about 4kg higher in nitrogen but smaller in phosphorus and almost the same in potassium. 3. Soil productivity was higher in IR667 but percent contribution of soil productivity to total productivity (field productivity) was lower. 4. Percent contribution of fertilization productivity to field productivity was 19 to 33, of which 5 was due to phosphorus and potassium. 5. The greater the soil productivity, the greater the field productivity becomes, but the much greater the percentage contribution of soil productivity becomes. 6. Fertility saturation point at which fertilization efficiency is nil, was propoesd as a soil productivity (or field productivity) limit.

• Composites Research
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• v.13 no.1
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• pp.1-10
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• 2000

During the compression molding process of the continuous fiber-reinforced polymeric composites, two main problems such as fiber-matrix separation and fiber orientation are produced by the difference of flow velocity. Molded parts are lead to be nonhomogeneous and anisotropic. As the mechanical property of the products are dependent on the separation and orientation, it is important to research the fiber mat structure and molding condition. If the fiber mat structure is changed by the increment of needling, the separation decreases and after compression molding the orientation is easily aligned. As it were, the compression moldability is good. But the defects as tears, thin thickness are produced in the products. Therefore, it is important to clarify the moldability in relation to the usage of products and the expenses of produce on the actual process. Therefore we must make the measurement methods that can define the moldability of products. In this research, the effects of the fiber mat structure(NP = 0, 5, 10, 25, 50 punches/$cm^2$) and the mold geometry($r_p$ = 1, 25, 50 mm) on the moldability of products were discussed. We investigated the case of one-dimensional flow in order to obtain the degree of nonhomogeneity and the fiber orientation function. In result, we could gain the correlation coefficient of the continuous fiber-reinforced polymeric composites. Also we experimented on the cup-type compression molding which was appeared the wrinkle on the flange part by the complex stress condition in order to gain the degree of nonhomogeneity and area ratio. In result, the moldability of products was expressed as the correlation coefficient and area ratio.

• Journal of the Korean Society of Marine Environment & Safety
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• v.13 no.4
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• pp.15-19
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• 2007

Mass mortalities of the farmed sea squirt, Halocynthia roretzi, occurred in August, 2006 along the Gyeongbuk coasts of the East Sea without any pathological symptom. Suspecting the No. 10 typhoon, Wukong, which had passed over the relevant locations in the middle of August, 2006 as a culprit of the mortalities, we compared water temperature stratifications of the affected coasts before and after the typhoon. Just prior to the typhoon, cold waters lower than $15^{\circ}C$ were stratified at depths deeper than 10m probably by a persisting upwelling of cold bottom waters. The typhoon enforced water off-coast waters into coasts for 5 days, replacing the cold waters at depths $10{\sim}20m$ which can be justified by Ekman Spiral Effect. The results were an increment of water temperature by $10^{\circ}C$ and more at depths where sea squirts were suspended. The sudden elevation of water temperature might drive the sea squirts under abnormal stress to mass mortality.

• Journal of Life Science
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• v.20 no.8
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• pp.1281-1286
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• 2010

Gaucher disease (GD) is caused by glucocerebrosidase functional deficiency and the most prevalent lysosomal storage disorder (LSD), with an incidence of about 1 in 20,000 new births. Resveratrol, one kind of phytoalexin, is a produced naturally by several plants and has anti-tumor, anti-aging, anti-inflammatory and neuro-protective effects. In this paper we provide the cellular protective effect of resveratrol in both type I and type II Gaucher disease cells. Resveratrol treatment did not show any significant change in the p21 and p53 mRNA expression level, however expression level of the p21 protein, a cell cycle arrest factor, shows significant increment in both types of Gaucher disease cells. These cell cycle arrest patterns were confirmed by both MTT assay measurement and microscopy detection. In comparison, expression level of poly ADP ribose polymerase (PARP), an apoptosis indicator protein, was significantly decreased in both type I and II Gaucher disease cells after treatment with resveratrol. This result indicates that resveratrol relievescellular apoptotic stress fromtype I and II Gaucher disease cells. Therefore, we demonstrate that resveratrol inhibits cell proliferation via p21 activity and activates cellular repair systems for Gaucher disease cells. Our results provide at least one of the molecular mechanisms of Gaucher disease and may allow the verification of potential drug targets for therapeutic trials.