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http://dx.doi.org/10.5352/JLS.2010.20.8.1281

Resveratrol Upregulates p21, Cell Cycle Regulator, in Gaucher Disease Cells  

Kim, Dong-Hyun (Department of biotechnology, Hoseo University)
Heo, Tae-Hwe (College of Pharmacy, The Catholic University of Korea)
Kim, June-Bum (Department of Pediatrics, Konyang University College of Medicine)
Kim, Sung-Jo (Department of biotechnology, Hoseo University)
Publication Information
Journal of Life Science / v.20, no.8, 2010 , pp. 1281-1286 More about this Journal
Abstract
Gaucher disease (GD) is caused by glucocerebrosidase functional deficiency and the most prevalent lysosomal storage disorder (LSD), with an incidence of about 1 in 20,000 new births. Resveratrol, one kind of phytoalexin, is a produced naturally by several plants and has anti-tumor, anti-aging, anti-inflammatory and neuro-protective effects. In this paper we provide the cellular protective effect of resveratrol in both type I and type II Gaucher disease cells. Resveratrol treatment did not show any significant change in the p21 and p53 mRNA expression level, however expression level of the p21 protein, a cell cycle arrest factor, shows significant increment in both types of Gaucher disease cells. These cell cycle arrest patterns were confirmed by both MTT assay measurement and microscopy detection. In comparison, expression level of poly ADP ribose polymerase (PARP), an apoptosis indicator protein, was significantly decreased in both type I and II Gaucher disease cells after treatment with resveratrol. This result indicates that resveratrol relievescellular apoptotic stress fromtype I and II Gaucher disease cells. Therefore, we demonstrate that resveratrol inhibits cell proliferation via p21 activity and activates cellular repair systems for Gaucher disease cells. Our results provide at least one of the molecular mechanisms of Gaucher disease and may allow the verification of potential drug targets for therapeutic trials.
Keywords
Resveratrol; Gaucher disease; P21; PARP; cell cycle;
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