• Journal of the Korean Society for Geothermal and Hydrothermal Energy
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• v.6 no.2
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• pp.9-14
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• 2010

The ground source heat pump system is increasingly being considered as an alternative to traditional heating and cooling systems to reduce the emission of ground house gases. In this paper, A series of numerical analysis for energy piles has been performed focusing on heat transfer efficiency, performance and thermal stress. Results of numerical analyses for the W-shape type shows more efficient heat exchange transfer than the coil type. From results of the thermo-mechanical analysis, it is shown that the concentration of thermal stress occurs around the circulating pipe and the interfaces between different materials. The largest deformation caused by thermal stress is observed in the energy pile.

• Proceedings of the KSR Conference
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• 2010.06a
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• pp.1430-1436
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• 2010

Tubular member is hollow and is an excellent source of structural member with great buckling resistance and tortional resistance. With its development and simplicity in structure, steel tubular truss has the ability to be structured in long span bridges, without a stiffener. Recently, it has been used in many countries in Europe, Canada, Japan, and the US with the help of international committees such as CIDECT(International Committee for the Development and Study of Tubular Structures and International Institute of Welding). The most important problem when using the tubular member is the fact that it is difficult to test the fatigue stress determined by nominal stress, since geometrical stress concentration occurs due to the welded joint's nod of complexity. The purpose of this study is to compare and examine current theories and widely applied Hot Spot stress determinations through finite element analysis, which is about welded joints of steel tubular truss. We would like to suggest a way of design practice which involves a bridge plan with rarely domestically used steel tubular truss` basic research data as well as considering the future of tubular member.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.34 no.5
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• pp.561-565
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• 2010

Stainless steel sheets are widely used as structural materials for the manufacture of railroad cars and commercial vehicles. These kinds structures used stainless steel sheets are commonly fabricated by using the gas welding. For the fatigue design of gas welded joints such as fillet joints and plug joints, it is necessary to obtain information on the stress distribution at the weldment and the fatigue strength of the gas welded joints. Moreover the influence of the geometrical parameters corresponding to the gas welded joints on the stress distribution and fatigue strength must be evaluated. ${\Delta}P-N_f$ curves were obtained from the data recorded in fatigue tests. Using these results, the ${\Delta}P-N_f$ curves were rearranged according the relation between $\Delta\sigma-N_f$ and the maximum stress at the edge of the fillet welded joint.

• Korea Journal of Cosmetic Science
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• v.1 no.1
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• pp.1-9
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• 2019

The identification of compounds with anti-senescence activity in cell culture system is a first step in aging research. Given that pyruvate can be used energy source by conversion to acetyl-CoA in mitochondria, and protects cultured cell from various stress-induced cell damage and cell death, synthetic media (e.g., DMEM) often includes 1 mM pyruvate, which is very higher than the pyruvate concentration in human blood (approximately 30 ��M). However, the use of medium containing high concentration of pyruvate is not suitable for screening anti-senescence compounds, because pyruvate also protects against the cellular senescence of primary human dermal fibroblasts (NHDFs) through NAD+ generated during conversion to lactate. In this study, four extracts, i.e., Sprouted seed and fruit complex, Poncirus trifoliata fruit extract, Jaum balancing complex, and Prunus mume extract were used for evaluation of different anti-senescence effect in the absence or presence of 0.1 mM pyruvate, similar to the physiological pyruvate concentration. The senescence in NHDFs cultured with DMEM in the presence of 0.1 mM pyruvate (approximately the physiological concentration in human blood) is accelerated, as observed in pyruvate deprivation conditions. The cytotoxicity of the Poncirus trifoliata fruit extract was protected by pyruvate, and Jaum balancing complex and Prunus mume extract had anti-senescence activity in the presence of 0.1 mM pyruvate, but not in the absence of pyruvate. Given that pyruvate is a powerful protector against both cytotoxicity and cellular senescence, the screening of candidate agents for anti-senescence in high pyruvate conditions using an in vitro cell culture system is not valid. Therefore, we recommend the use of a low concentration of pyruvate to evaluate the anti-senescence effects of candidates, which is more similar to in vivo aging conditions than excessive stress-induced senescence models, to exclude the effect of excessive pyruvate in vitro.

• Journal of Mechanical Science and Technology
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• v.17 no.3
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• pp.440-448
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• 2003

In the present paper, numerical simulations of buoyant plume dispersion in a neutral and stable atmospheric boundary layer have been carride out. A Lagrangian Stochastic Model (LSM) with a Non-Linear Eddy Viscosity Model (NLEVM) for turbulence is used to generate a Reynolds stress field as an input condition of dispersion simulation. A modified plume-rise equation is included in dispersion simulation in order to consider momentum effect in an initial stage of plume rise resulting in an improved prediction by comparing with the experimental data. The LSM is validated by comparing with the prediction of an Eulerian Dispersion Model (EDM) and by the measured results of vertical profiles of mean concentration in the downstream of an elevated source in an atmospheric boundary layer. The LSM predicts accurate results especially in the vicinity of the source where the EDM underestimates the peak concentration by 40% due to inherent limitations of gradient diffusion theory. As a verification study, the LSM simulation of buoyant plume dispersions under a neutral and stable atmospheric condition is compared with a wind-tunnel experiment, which shows good qualitative agreements.

• Journal of Welding and Joining
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• v.31 no.6
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• pp.96-106
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• 2013

In this study, the effect of laser source($CO_2$ and Nd:YAG) on the microstructure and tensile properties of laser welded Al-Si coated boron steel(1.2mmt) was investigated with before and after hot-stamping. In case of as welds condition, fracture occurred in base metal unrelated to the laser source. It could be explained that tensile strength of fusion zone composed of martensite and bainite is higher than that of base metal that contains a lot of ferrite despite dilution of Al and Si from coating layer to fusion zone. In case of hot-stamping condition, the fracture occurred in fusion zone irrelevant to laser source and the tensile strength was lower than hot stamped base metal. In the $CO_2$ laser welds, $Fe_3$(Al,Si) formed near the bond line was transformed into ferrite during hot-stamping. Therefore tensile strength of bond line is lower than that of base metal and center of fusion zone and the fracture occurred in the bond line. On the other hand, in the Nd:YAG laser welds, the higher concentration of Al formed the ferrite in the fusion zone during hot-stamping treatment. Also, the thickness of centerline was thinner than that of base metal. Therefore, it is considered that fracture occurred in centerline of fusion zone due to effect of concentration stress, and it leaded to a lower tensile strength and elongation.

• Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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• 1997.04a
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• pp.74-79
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• 1997

Most of the results of recent research for the influence of indentation on rolling contact fatigue has been carried out with high strength material under the point contact. The plastic lug around the indent also has been known as a source of stress concentration. This study is undertaken to analyze the influence of indentation on rolling contact fatigue with low strength material under the line contact. The results in this study show that the plastic flow around indentation by rolling friction has a major influence, differed from the results of high strength material. And the change of residual stress and half-value breadth measured to the failure by X-ray diffraction can be identified to predict the rolling contact fatigue life of indented materials.

• Journal of Animal Science and Technology
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• v.47 no.2
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• pp.253-260
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• 2005

This study was conducted to utilize the wastes produced from thinning the forest as a roughage source for ruminants. Four ruminally cannulated Korean steers were used to investigate the ruminal fermentation characteristics and blood concentrations of stress-related hormones. Treatments were composed of the rice straw only (Control) and the 30% pine silage plus 70% rice straw(Pine silage) as roughages. The experiment was conducted with four replicates by a double tum over design. For sampling of blood under high temperature steers were accommodated in respiratory chamber. Concentrations of ruminal pH, volatile fatty acids and ammonia were not affected by substitution of pine silage. Mean concentration of blood thyroxine was significantly(P < 0.01) lower in the pine silage than the control. However, blood cortisol concentration was not affected by substitution of pine silage, even though it was significantly(P < 0.05) decreased after exposure for 6 hrs in high temperature. It was concluded that the pine silage using the wastes produced from thinning the forest could be use as a substitute roughage for reduction of heat stress in ruminant.

• KSBB Journal
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• v.16 no.4
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• pp.415-419
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• 2001

To produce staphylokinase (SAK) in B. subtilis WB700, media optimization was carried out and the operation of batch and fed-batch fermentation were compared. Tryptone is a good nitrogen source and its optimum concentration in modified super rich(MSR) media is 15 g/L. When glucose is used as a limiting carbon source in the MSR media, 5 g/L of an optimum glucose concentration was identified for the SAK production under the control of P43 promoter. As the expression of P43 promoter is controlled by the limitation of oxygen, the SAK production was controlled at the 30% DO level in the fed-batch fermentation. Unexpectedly, batch fermentation using MSR media showed 1.5 times higher yield of SAK than that of the fed-batch fermentation. The main cause of the results comes from not achieving higher cell concentration in the fed-batch fermentation and the optimum expression level of P43 promoter under oxygen or nutrient limitations. We could not achieve the increase in cell concentration by any means in batch culture as well as fed-batch culture. The highest yield in the batch culture was 2880 units of SAK activity and 455 mg/L of secreted SAK.

• KSBB Journal
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• v.9 no.2
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• pp.140-146
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• 1994

Aureobasidium pullulans produced high concentration of polyols extracellularly in the media of sucrose, glucose and mannose as sole carbon source. Mannitol was the main polyol produced during the late exponential and stationary phases of growth together with small quantities of glycerol. Sucrose and glucose were rather rapidly metabolized to mannitol among carbon sources examined where the initial glucose concentration showed no difference in the amount of mannitol. In contrast 20%(w/v) of sucrose was the most appropriate concentration tested. However, the yield of mannitol based on substrate used($Y_{p/s}$) was independent on the initial concentration, and the mean value of mannitol yield in 10% glucose and sucrose media was 0.144 and 0.188, respectively. Mannitol production was reduced in response to an elevated water stress imposed by salts within the range from 0.25 to IM of NaCl or KCl as stress solutes. However, glycerol contents and its ratio to mannitol were increased at the conditions of high salinity. Based on the results, extracellular mannitol produced by A. pullulans probably resulted partly from osmoregulation(in case of glycerol) and mainly from, as known to occur in most of fungi, enzymatic reduction of the corresponding hexoses through phosphate pathway.