• Composites Research
• /
• v.36 no.3
• /
• pp.154-161
• /
• 2023

This study explored the feasibility of replacing a metal link with a carbon fiber/epoxy composite link and assessed its capacity to withstand a given load condition using failure criteria. The micromechanics of failure (MMF) criterion was employed to predict the failure mode of the composite material, and mechanical tests were conducted to obtain reference strength parameters for MMF. The findings revealed that the stress distribution was concentrated near the hole, and weaknesses were found around the hole and at the end of the link under bending conditions. Based on the failure index, matrix tensile failure was predicted at the end of the link, and fiber compression failure occurred near the hole. The methods and results obtained from this study can provide valuable guidelines for assessing the safety of composite materials under specific load conditions when replacing metal parts with carbon fiber/epoxy composites to achieve weight reduction.

• BMB Reports
• /
• v.32 no.2
• /
• pp.161-167
• /
• 1999

The role of oxidative stress in the initiation and the complication of diabetes was examined by monitoring blood glucose increase and oxidative DNA damage in rats treated with alloxan or streptozotocin (STZ). Oxidative DNA damage was assessed by quantitating 8-oxo-2'-deoxyguanosine ($oxo^8dG)$ excreted in urine and the $oxo^8dG$ accumulated in pancreas DNA. Both alloxan and STZ treatments resulted in an abrupt increase in blood glucose and significant increases in urinary and pancreatic $oxo^8dG$. Pretreatment of buthionine sulfoximine (BSO), a glutathione-depleting agent, slightly potentiated the increase of blood glucose and urinary $oxo^8dG$ in the alloxan- and STZ-treated rats. Furthermore, the BSO pretreatment caused significant amplification of pancreatic $oxo^8dG$ increase in the rats. On the other hand, pretreatment with 1,10- phenanthroline (o-phen), a chelator of divalent cations, showed different results between alloxan- and STZ-treated rats. The o-phen pretreatment completely blocked diabetes and the increase of $oxo^8dG$ by alloxan treatment, while it potentiated the increase of blood glucose and $oxo^8dG$ by STZ treatment. The results demonstrate that the causative effect of alloxan on diabetes may be the generation of reactive oxygen species through a Fenton type reaction, but that of STZ may not.

• BMB Reports
• /
• v.36 no.5
• /
• pp.427-432
• /
• 2003

Jute is the principal coarse fiber for commercial production and use in Bangladesh. Therefore, the development of a high-yielding and environmental-stress tolerant jute variety would be beneficial for the agro economy of Bangladesh. Two molecular fingerprinting techniques, random-amplified polymorphic DNA (RAPD) and amplified-fragment length polymorphism (AFLP) were applied on six jute samples. Two of them were cold-sensitive varieties and the remaining four were cold-tolerant accessions. RAPD and AFLP fingerprints were employed to generate polymorphism between the cold-sensitive varieties and cold-tolerant accessions because of their simplicity, and also because there is no available sequence information on jute. RAPD data were obtained by using 30 arbitrary oligonucleotide primers. Five primers were found to give polymorphism between the varieties that were tested. AFLP fingerprints were generated using 25 combinations of selective-amplification primers. Eight primer combinations gave the best results with 93 polymorphic fragments, and they were able to discriminate the two cold-sensitive and four cold-tolerant jute populations. A cluster analysis, based on the RAPD and AFLP fingerprint data, showed the population-specific grouping of individuals. This information could be useful later in marker-aided selection between the cold-sensitive varieties and cold-tolerant jute accessions.

• Environmental Analysis Health and Toxicology
• /
• v.24 no.2
• /
• pp.107-117
• /
• 2009

Metal pollution of aquatic ecosystems is a problem of economic and health importance. Information regarding molecular responses to metal exposure is sorely needed in order to identify potential biomarkers. To determine the effects of heavy metals on chironomids, the full-length cDNA of alcohol dehydrogenase (ADH3) from Chironomus riparius was determined through molecular cloning and rapid amplification of cDNA ends (RACE). The expression of ADH3 was analyzed under various cadmium and copper concentrations. A comparative and phylogenetic study among different orders of insects and vertebrates was carried out through analysis of sequence databases. The complete cDNA sequence of the ADH3 gene was 1134 bp in length. The sequence of C. riparius ADH3 shows a low degree of amino acid identity (around 70%) with homologous sequences in other insects. After exposure of C. riparius to various concentrations of copper, ADH3 gene expression significantly decreased within 1 hour. The ADH3 gene expression was also suppressed in C. riparius after cadmium exposure for 24 hour. However, the effect of cadmium on ADH3 gene expression was transient in C. riparius. The results show that the suppression of ADH3 gene by copper exposure could be used as a possible biomarker in aquatic environmental monitoring and imply differential toxicity to copper and cadmium in C. riparius larvae.

• Proceedings of the Earthquake Engineering Society of Korea Conference
• /
• 2001.04a
• /
• pp.100-107
• /
• 2001

One major problem in the model testing is the boundary effect and size effect caused by the limit in the size of the container. To overcome this problem, various types of laminar boxes are gradually manufactured and used in the shaking table test, which ideally has zero stiffness to horizontal shear. In this study, a small-scale laminar box is manufactured, which is composed of 6 thin aluminum rectangular hollow plates, and its inside dimensions are 300 mm length by 200 mm width by 350 mm depth. Shaking table tests are performed both with the laminar box and the rigid box under the same conditions, where displacements and accelerations are measured at various points of the box and model ground. As result of analyzing the measured data, during the propagation of input seismic motion from the bottom to the ground surface, the relative displacement of the model ground and the amplification of acceleration is hardly amplified in the rigid box. Because of the effect of stress waves reflecting from the rigid wall, the acceleration is slightly decreased at the edge in the rigid box. The laminar box, manufactured in this study, has a problem in that the soil behavior at the edge of ground surface is affected by the inertia force of the top layer due to its excessive self-weight.

• Journal of Ocean Engineering and Technology
• /
• v.27 no.4
• /
• pp.98-106
• /
• 2013

Seismic fragility curves for an offshore wind-turbine structure were obtained. The dynamic response of an offshore wind turbine was analyzed by considering the nonlinear behavior of layered soil and the added mass effect due to seawater. A pile-soil interaction effect was considered by using nonlinear p-y, t-z curves. In the analysis, the amplification effect of ground acceleration through layered soil was considered by applying ground motion to each of the soil layers. The vertical variation in ground motion was found by one-dimensional free-field analysis of ground soils. Fragility curves were determined by damage levels in terms of tower stress and nacelle displacements that were found from static pushover analysis of the wind-turbine structure.

• Journal of computational fluids engineering
• /
• v.10 no.2
• /
• pp.48-53
• /
• 2005

This work discusses the numerical analysis, the design and experimental test of the SMA actuator along with its capabilities and limitations. Convective heating and cooling using water actuate the SMA(Shape memory alloy) element of the actuator. The fuel such as propane, having a high energy density, is used as the energy source for the SMA actuator in order to increase power and energy density of the system, and thus in order to obviate the need for electrical power supplies such as batteries. The system is composed of a pump, valves, bellows, a heater(burner), control unit and a displacement amplification device. The experimental test of the SMA actuator system results in 150 MPa stress(force : 1560 N) with $3\%$ strain and 0.5 Hz. actuation frequency. The actuation frequency is compared with the prediction obtained from numerical analysis. For the designed SMA actuator system, the results of numerical analysis were utilized in determining design parameters and operating conditions.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.21 no.1
• /
• pp.21-26
• /
• 2001

To develop transgenic orchardgrass resistant to reactive oxygen species produced from environmental stresses, a vector with the cytosolic glutathione reductase cDNA (BcGRl) from Chinese cabbage was constructed under the control of the cauliflower mosaic virus 35S promoter and was introduced into orchardgrass using Agrobacterium tumefaciens EHA101. Transgenic plants from hygromycin-selected calli of orchardgrass did not show any morphological difference from wild-type plants. The results of PCR amplification and genomic Southern blot analysis confirmed the integration of foreign gene into the chromosome of transgenic orchardgrass. Northern blot analysis with total RNA from leaves also confirmed the constitutive expression of BcGR1 in transgenic orchardgrass.

• Journal of Marine Bioscience and Biotechnology
• /
• v.2 no.4
• /
• pp.263-266
• /
• 2007

Vibrio vulnificusis a causative agent of serious diseases in humans resulting from the contact of wound with seawater or consumption of raw seafood. Several studies aimed at detecting V. vulnificus have targeted vvh as a representative virulence toxin gene belonging to the bacterium. In this study, we targeted the rpoS gene, a general stress regulator, to detect V. vulnificus. PCR specificity was identified by amplification of 8 V. vulnificus templates and by the loss of a PCR product with 36 non-V. vulnificus strains. The PCR assay had the 273-bp fragment and the sensitivity of 10 pg DNA from V. vulnificus. SYBR Green I-based real-time PCR assay targeting the rpoS gene showed a melting temperature of approximately $84^{\circ}C$ for V. vulnificus strains. The minimum level of detection by real-time PCR was 2 pg of purified genomic DNA, or $10^3$ V. vulnificus cells from pure cultured broth and $10^3$ cells in 1g of oyster tissue homogenates. These data indicate that real-time PCR is a sensitive, species-specific, and rapid method for detecting this bacterium using the rpoS gene in pure cultures and in infected oyster tissues.

• Journal of the Earthquake Engineering Society of Korea
• /
• v.13 no.3
• /
• pp.29-38
• /
• 2009

n the event of an earthquake, additional stresses can occur in the continuous welded rails (CWR) of High-speed railway (HSR) bridges due to relative displacements at expansion joints, and this stress can cause derailment. The amplification of ground motion occurs as a result of site effects, and this is pronounced at the site of a soft surface soil layer and of a rigid surface soil layer over a soft one. As a result, the amplified ground motion leads to an amplified seismic response in HSR bridges. A change in bridge pier height affects the seismic behavior of the bridge. A HSR bridge with gravel ballast tracks will show different dynamic behavior during an earthquake than one with concrete ballast tracks. The seismic responses of HSR bridges and their CWR are analyzed considering the derailment-inducing factors.