• Composites Research
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• 제36권3호
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• pp.154-161
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• 2023

본 연구에서는 경량화를 위해 금속 링크를 탄소섬유/에폭시 복합재 링크로 대체하고자 파손 기준을 이용하여 복합재 링크가 주어진 하중 조건을 견딜 수 있는지를 평가하였다. 복합재의 파손 양상을 예측하기 위해 MMF 기준을 이용하였고, 기계적 시험을 수행하여 MMF의 기준 강도 파라미터를 구하였다. 연구결과 링크의 구멍 주위에서 응력집중이 발생하였고, 특히 굽힘 하중을 받을 때 링크 끝단과 구멍 주위에서 취약함이 드러났다. 파손 지수로부터 파손 양상을 예측하였고 매트릭스 인장 파손이 링크 끝단에서, 그리고 구멍 주위에서는 섬유의 압축 파손이 예측되었다. 본 연구를 통해 확보된 방법과 결과는 경량화를 위해 금속 부품을 탄소섬유/에폭시 복합재로 대체할 때 특정 하중 조건 하에서 복합재의 안전성을 평가하는 유용한 지침으로 활용할 수 있을 것이다.

• BMB Reports
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• 제32권2호
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• pp.161-167
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• 1999

The role of oxidative stress in the initiation and the complication of diabetes was examined by monitoring blood glucose increase and oxidative DNA damage in rats treated with alloxan or streptozotocin (STZ). Oxidative DNA damage was assessed by quantitating 8-oxo-2'-deoxyguanosine ($oxo^8dG)$ excreted in urine and the $oxo^8dG$ accumulated in pancreas DNA. Both alloxan and STZ treatments resulted in an abrupt increase in blood glucose and significant increases in urinary and pancreatic $oxo^8dG$. Pretreatment of buthionine sulfoximine (BSO), a glutathione-depleting agent, slightly potentiated the increase of blood glucose and urinary $oxo^8dG$ in the alloxan- and STZ-treated rats. Furthermore, the BSO pretreatment caused significant amplification of pancreatic $oxo^8dG$ increase in the rats. On the other hand, pretreatment with 1,10- phenanthroline (o-phen), a chelator of divalent cations, showed different results between alloxan- and STZ-treated rats. The o-phen pretreatment completely blocked diabetes and the increase of $oxo^8dG$ by alloxan treatment, while it potentiated the increase of blood glucose and $oxo^8dG$ by STZ treatment. The results demonstrate that the causative effect of alloxan on diabetes may be the generation of reactive oxygen species through a Fenton type reaction, but that of STZ may not.

• BMB Reports
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• 제36권5호
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• pp.427-432
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• 2003

Jute is the principal coarse fiber for commercial production and use in Bangladesh. Therefore, the development of a high-yielding and environmental-stress tolerant jute variety would be beneficial for the agro economy of Bangladesh. Two molecular fingerprinting techniques, random-amplified polymorphic DNA (RAPD) and amplified-fragment length polymorphism (AFLP) were applied on six jute samples. Two of them were cold-sensitive varieties and the remaining four were cold-tolerant accessions. RAPD and AFLP fingerprints were employed to generate polymorphism between the cold-sensitive varieties and cold-tolerant accessions because of their simplicity, and also because there is no available sequence information on jute. RAPD data were obtained by using 30 arbitrary oligonucleotide primers. Five primers were found to give polymorphism between the varieties that were tested. AFLP fingerprints were generated using 25 combinations of selective-amplification primers. Eight primer combinations gave the best results with 93 polymorphic fragments, and they were able to discriminate the two cold-sensitive and four cold-tolerant jute populations. A cluster analysis, based on the RAPD and AFLP fingerprint data, showed the population-specific grouping of individuals. This information could be useful later in marker-aided selection between the cold-sensitive varieties and cold-tolerant jute accessions.

• Environmental Analysis Health and Toxicology
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• 제24권2호
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• pp.107-117
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• 2009

Metal pollution of aquatic ecosystems is a problem of economic and health importance. Information regarding molecular responses to metal exposure is sorely needed in order to identify potential biomarkers. To determine the effects of heavy metals on chironomids, the full-length cDNA of alcohol dehydrogenase (ADH3) from Chironomus riparius was determined through molecular cloning and rapid amplification of cDNA ends (RACE). The expression of ADH3 was analyzed under various cadmium and copper concentrations. A comparative and phylogenetic study among different orders of insects and vertebrates was carried out through analysis of sequence databases. The complete cDNA sequence of the ADH3 gene was 1134 bp in length. The sequence of C. riparius ADH3 shows a low degree of amino acid identity (around 70%) with homologous sequences in other insects. After exposure of C. riparius to various concentrations of copper, ADH3 gene expression significantly decreased within 1 hour. The ADH3 gene expression was also suppressed in C. riparius after cadmium exposure for 24 hour. However, the effect of cadmium on ADH3 gene expression was transient in C. riparius. The results show that the suppression of ADH3 gene by copper exposure could be used as a possible biomarker in aquatic environmental monitoring and imply differential toxicity to copper and cadmium in C. riparius larvae.

• 한국지진공학회:학술대회논문집
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• 한국지진공학회 2001년도 춘계학술대회 논문집
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• pp.100-107
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• 2001

One major problem in the model testing is the boundary effect and size effect caused by the limit in the size of the container. To overcome this problem, various types of laminar boxes are gradually manufactured and used in the shaking table test, which ideally has zero stiffness to horizontal shear. In this study, a small-scale laminar box is manufactured, which is composed of 6 thin aluminum rectangular hollow plates, and its inside dimensions are 300 mm length by 200 mm width by 350 mm depth. Shaking table tests are performed both with the laminar box and the rigid box under the same conditions, where displacements and accelerations are measured at various points of the box and model ground. As result of analyzing the measured data, during the propagation of input seismic motion from the bottom to the ground surface, the relative displacement of the model ground and the amplification of acceleration is hardly amplified in the rigid box. Because of the effect of stress waves reflecting from the rigid wall, the acceleration is slightly decreased at the edge in the rigid box. The laminar box, manufactured in this study, has a problem in that the soil behavior at the edge of ground surface is affected by the inertia force of the top layer due to its excessive self-weight.

• 한국해양공학회지
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• 제27권4호
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• pp.98-106
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• 2013

Seismic fragility curves for an offshore wind-turbine structure were obtained. The dynamic response of an offshore wind turbine was analyzed by considering the nonlinear behavior of layered soil and the added mass effect due to seawater. A pile-soil interaction effect was considered by using nonlinear p-y, t-z curves. In the analysis, the amplification effect of ground acceleration through layered soil was considered by applying ground motion to each of the soil layers. The vertical variation in ground motion was found by one-dimensional free-field analysis of ground soils. Fragility curves were determined by damage levels in terms of tower stress and nacelle displacements that were found from static pushover analysis of the wind-turbine structure.

• 한국전산유체공학회지
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• 제10권2호
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• pp.48-53
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• 2005

This work discusses the numerical analysis, the design and experimental test of the SMA actuator along with its capabilities and limitations. Convective heating and cooling using water actuate the SMA(Shape memory alloy) element of the actuator. The fuel such as propane, having a high energy density, is used as the energy source for the SMA actuator in order to increase power and energy density of the system, and thus in order to obviate the need for electrical power supplies such as batteries. The system is composed of a pump, valves, bellows, a heater(burner), control unit and a displacement amplification device. The experimental test of the SMA actuator system results in 150 MPa stress(force : 1560 N) with $3\%$ strain and 0.5 Hz. actuation frequency. The actuation frequency is compared with the prediction obtained from numerical analysis. For the designed SMA actuator system, the results of numerical analysis were utilized in determining design parameters and operating conditions.

• 한국초지조사료학회지
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• 제21권1호
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• pp.21-26
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• 2001

환경 스트레스에 의해 야기되는 활성 산소종에 의한 피해에 내성을 가지는 목초의 개발을 위하여 오차드그래스의 배반 조직 유래의 캘러스에 배추유래의 cytosolic glutathione reductase 유전자(BcGRl)를 Agrobucterium tumefaciens EHA101을 매개로 형질전환시켰다. Hygromcin으로 선발된 캘러스로부터 재분화된 식물체는 야생형과 비교하여 형태적으로 차이를 나타내지 않았다. PCR 및 Southern blot 분석을 통하여 형질전환 식물체의 염색체 내에 BcGRl 유전자가 integration 되었음을 확인하였다. 오차드그래스의 잎으로부터 total RNA를 분리하여 Northern blot 분석을 실시한 결과, 도입된 유전자가 형짙전환 식물체 내에서 지속적으로 발현된다는 것을 확인하였다.

• 한국해양바이오학회지
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• 제2권4호
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• pp.263-266
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• 2007

Vibrio vulnificusis a causative agent of serious diseases in humans resulting from the contact of wound with seawater or consumption of raw seafood. Several studies aimed at detecting V. vulnificus have targeted vvh as a representative virulence toxin gene belonging to the bacterium. In this study, we targeted the rpoS gene, a general stress regulator, to detect V. vulnificus. PCR specificity was identified by amplification of 8 V. vulnificus templates and by the loss of a PCR product with 36 non-V. vulnificus strains. The PCR assay had the 273-bp fragment and the sensitivity of 10 pg DNA from V. vulnificus. SYBR Green I-based real-time PCR assay targeting the rpoS gene showed a melting temperature of approximately $84^{\circ}C$ for V. vulnificus strains. The minimum level of detection by real-time PCR was 2 pg of purified genomic DNA, or $10^3$ V. vulnificus cells from pure cultured broth and $10^3$ cells in 1g of oyster tissue homogenates. These data indicate that real-time PCR is a sensitive, species-specific, and rapid method for detecting this bacterium using the rpoS gene in pure cultures and in infected oyster tissues.

• 한국지진공학회논문집
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• 제13권3호
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• pp.29-38
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• 2009

지진 발생시 교량 상판 연결부의 상대변위로 레일에 발생하는 부가응력으로 인한 레일의 좌굴과 파손은 열차의 탈선을 유발할 수 있다. 교량의 지진응답 증가에 영향을 미치는 지반의 탈선취약요소와 교량의 구조적인 요소를 고려하여 고속철도교량 장대레일의 지진응답을 평가하였다. 연약층이 있는 지반, 상하역전형 지반과 같은 지반 탈선취약요소를 고려하기 위해서 지반을 평행층상지반으로 모델링하여 부지효과가 고려된 자유장운동을 구하고 이를 입력지진으로 사용하였다. 교각 높이나 적용 도상을 변화시키면서 구조적 특성이 레일의 지진응답에 미치는 영향을 분석하였다.