• Asian Pacific Journal of Cancer Prevention
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• 제15권14호
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• pp.5895-5900
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• 2014

Background: Metabolic syndrome (MS) is a cluster of complicated disorders caused by the interactive influencing factors of heredity and environment, which predisposes to many cnacers. Results from epidemic research indicate that stress is tightly related to the pathogenesis of MS and neoplasia. This paper aims to investigate the association between psychological stress and MS with respect to the tumor necrosis factor alpha (TNF${\alpha}$) and neuropeptide Y (NPY) genes in the Han and Hui ethnic groups. Methods: All subjects for this case-control study matched strict enrollment criteria (nationality, gender and age) and lived in the city of Wu Zhong of Ningxia Province in China. The enrolled group contained 102 matched pairs of Hui ethnic individuals and 98 matched pairs of Han ethnic individuals. Enrolled subjects completed the general Symptom Checklist-90 (SCL-90). The TNF${\alpha}$-308G/A variant and NPYrs16147 polymorphism were detected in case (81 males, 119 females) and control (81 males, 119 females) groups by polymerase chain reaction (PCR) amplification. Results: Nine factors of the SCL-90 were found to be statistically different (p<0.05) between case and control groups. The homozygous mutant genotype (AA) and the mutant allele (A) of the TNF${\alpha}$-308G/A gene were less frequently observed in the control population compared to the case group. The odds ratio (95% confidence interval) in "Allele" for MS was 2.28 (1.47-3.53), p=0.0001, while "OR" was 1.11 (0.83-1.47), p=0.15, for the NPYrs16147 gene polymorphism. Conclusions: Psychological stress has been positively associated with MS. A previous study from our group suggested there were differences in the level of psychological stress between Hui and Han ethnic groups. Furthermore, we found that the stress-related TNF${\alpha}$ gene was associated with MS for both Han and Hui ethnic groups. In contrast, NPY may be a possible contributor to MS and associated cancer for the Han ethnic group.

• 한국지반공학회논문집
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• 제26권4호
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• pp.27-36
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• 2010

사질토의 상태정수를 현장시험으로부터 추정하기 위해 실내 챔버에 조성된 부산사에 대해 딜라토미터 시험을 수행하였다. 시험결과, 수평증폭계수($K_D/K_0$)와 상태정수($\Psi$)의 관계는 반대수축상에서 선형관계를 보였으나, 응력이력에 의한 잔류수평응력의 영향으로 과압밀 시료가 정규압밀 시료에 비하여 동일한 상태정수에서 수평증폭계수의 값이 작게 평가되었다. 유효응력으로 정규화된 딜라토미터 계수($E_D/\sigma_m'$)와 상태정수의 관계는 반대수축상에서 선형관계를 만족하였으며, 다소의 분산은 있으나 응력이력에 관계없이 하나의 관계식으로 표현 기능하였다. 다만 응력수준의 영향으로 과압밀 상태보다 정규압밀 상태의 분산이 약간 크게 발생되었으며 구속압이 증가할수록 관계곡선이 하향 이동하는 경향이 나타났다. 기존에 연구된 Ticino sand 및 Toyoura sand와 비교한 결과, $K_D/K_0-\Psi$ 관계는 혼재되어 분포하였으나, $E_D/\sigma_m'-\Psi$ 관계는 시료 압축성의 영향으로 Toyoura sand가 Ticino snad 및 부산사의 상부에 위치하였다.

• 한국인터넷방송통신학회논문지
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• 제16권3호
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• pp.187-201
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• 2016

본 연구는 20세에서 23세 사이의 대학생 7명을 대상으로 스마트폰 사용에 대한 경험 구조를 분석하고자 총 4회에 걸친 인터뷰를 실시하였다. Giorgi의 현상학적방법을 이용하여 분석한 결과 4개의 일반적 구조문장과 28개의 주제로 작성할 수 있었다. 일반적 구조문장은 스마트폰 사용의존, 스마트폰 사용현상, 스마트폰 사용결과, 스트레스와 스마트폰과의 관계이었고 주제는 양가감정, 스마트폰에 대한 긍정적 감정, 분리불안, 스마트폰에 대한 부정적 감정, 스마트폰에 의존되어 있는 감정, 스마트폰과의 일체감, 생활의 보조 역할, 깊게 삶에 관여함, 일상생활에 유용함, 학습 소통 휴식과 건강관리, 인간관계 유지, 가장 중요한 존재, 깊이가 얕은 관계의 속상함, 대인관계 방해, 부정적인 대인관계 형성, 사적인 부분 침해, 부정확한 인맥 구축, 어쩔 수 없이 참여, 개선하지 않으려는 인간관계, 집중력 저하, 학업에 방해, 학업에 도움, 심층학습 성취에는 한계적 도움, 스트레스 도피 공간, 스트레스 증폭, 강박적 집중, 괴리감, 시간낭비이었다. 결론적으로, 현재 대학생에게 스마트폰의 사용은 스마트폰에 의존하게 되었고, 인간관계와 학업에 있어서 부정적인 영향을 미치고 있었으며, 스트레스를 증폭시켰다. 이에 따라 스마트폰 사용의 원칙이 필요하고, 합리적인 스마트폰 사용을 위한 문화 구축을 제언한다.

• 정신신체의학
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• 제17권1호
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• pp.8-14
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• 2009

연구목적 : 본 연구의 목적은 만성전립선염 환자들을 대상으로 스트레스의 다양한 측면들을 조사하여 만성전립선염과 스트레스와의 연관성을 알아보고자 함이다. 방법 : 비세균성 만성전립선염으로 진단받은 32명의 환자군과 건강한 성인남자 64명을 대상으로 하여 자기보고식 설문지를 작성하도록 하였다. 스트레스 인자에 대한 지각, 스트레스 반응 및 대응전략은 각각 스트레스 지각척도(Global Assessment of Recent Stress Scale), 스트레스 반응척도(Stress Response Inventory) 및 대처방식 체크리스트(Ways of Coping Checklist)를, 전립선염의 증상 정도는 미국립보건원 만성전립선염 증상지수(NIH-Chronic Prostatitis Symptom Index)를 이용하여 평가하였다. 결과 : 만성전립선염 환자는 대조군에 비하여 대인관계의 변화, 질병 및 상해, 경제적인 문제와 관련된 스트레스인자 지각점수가 높았고, 환자군이 대조군에 비해 스트레스 반응이 전체적으로 높게 나타났으며, 스트레스 대처방식에서는 적극적 대처와 소극적 대처 모두 환자군이 대조군에 비해 전체적으로 스트레스 대처수준이 낮게 관찰되었다. 전립선염 증상 정도가 심한 환자일수록 스트레스에 대한 반응에서 우울의 경향을 더 나타내었고 (p=0.039), 스트레스 대처방식에서는 문제중심적 대처방식(p=0.000)과 사회적 지지의 추구(p=0.017)와 유의한 음의 상관성을 보였다. 결론 : 만성전립선염 환자들은 다양한 영역의 스트레스를 경험하고, 높은 스트레스 반응을 보이며, 이에 대한 대처전략은 부족하였다. 이와 같은 결과는 만성전립선염 환자의 치료에 있어서 동반된 스트레스 인자의 평가 및 조절, 스트레스에 대한 반응과 대처방식의 효율성 증진 등이 효과적일 수 있음을 시사한다.

• 한국융합학회논문지
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• 제8권12호
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• pp.31-38
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• 2017

본 연구는 콩과 식물인 비수리의 유용유전자를 NGS (Next Generation Sequencing)와 분자생물학의 융합적인 연구를 통해 분리하고 가능성을 알아보고자 시행하였다. 비수리는 자원식물이지만 많은 유용물질을 가지고 있다. 특히 항당뇨 기능을 하는 D-pinitol을 많이 함유하고 있는데 아직까지 비수리에서 D-piniol의 생합성에 관련된 유전자가 분리 되지 않았다. 비수리에 비생물학적 스트레스(가뭄)를 처리하고 처리하지 않은 대조군과 같이 total RNA를 추출한 후에 library를 만들어 NGS를 실시하였다. 이를 통해 D-pinitol 생합성에 관련된 유전자들을 분리하여 in silico 상에서 염기서열을 확인하였다. 이를 뒷받침하기 위해 Blast 프로그램을 사용하여 D-pinitol 생함성에 관여하는 ononitol epimerase를 확인하였고 in vitro 상에서도 RT-PCR을 통해 유전자 발현이 증가됨을 확인함으로써 융합적 연구를 통해 유전자를 찾고 분리하여 발현양상을 확인하였다.

• 환경생물
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• 제34권2호
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• pp.97-106
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• 2016

$Na^+/K^+$-ATPase is a membrane protein and plays a key role in osmotic regulation in living organisms. In the present study, a cDNA sequence encoding the $Na^+/K^+$-ATPase alpha subunit from the monogonont rotifer, Brachionus koreanus was cloned by rapid amplification of cDNA ends technique. To investigate the role of this enzyme in osmotic stress, enzymatic activities of $Na^+/K^+$-ATPase were measured after exposure to different salinities for 48 h. The full-length Bk $Na^+/K^+$-ATPase cDNA was 3069 bp-long, encoding a 1022-amino acid polypeptide. Bk $Na^+/K^+$-ATPase possesses eight membrane spanning regions and five conserved domains. Phylogenetic analysis showed that Bk $Na^+/K^+$-ATPase had high identity with those of other species, and was closely clustered with other Brachionus sp. These findings indicate that this protein was conserved both structurally and functionally. B. koreanus $Na^+/K^+$-ATPase activity was stimulated in both hyposaline (6 psu) and hypersaline (32 psu) conditions, suggesting that this protein may play a role in osmoregulation. This study would provide better understanding of the physiology of B. koreanus and this enzyme may be useful as a molecular marker for evaluation of osmotic stress in aquatic environment.

• Journal of Microbiology and Biotechnology
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• 제28권7호
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• pp.1185-1198
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• 2018

The nucleosomal organization of chromatin using histone proteins is a fundamental and ubiquitous feature of eukaryotic nuclei, with the major exception of dinoflagellates. Although a number of recent genomic and transcriptomic analyses have detected numerous histone genes in dinoflagellates, little is known about their expression. Here in, we aimed to investigate the expression pattern of histone genes under nutritional stress, and an attempt was made to detect histone expression at the protein level in Alexandrium pacificum. The presence of histones at the mRNA level was confirmed in this study by the amplification, cloning, and sequencing of 10 different genes. Relative expression profiling of these genes under different growth conditions was determined with real-time PCR and revealed considerable levels of histone transcription in nutritionally stressed cells. We were unable to detect the expression of histones at the protein level even after immunodetection and analysis using mass spectrometry, although a histone-like protein was detected as a major nuclear component. A. pacificum expresses multiple variants of histone, and protein sequences revealed both conservation and divergence with respect to other eukaryotes. We concluded that A. pacificum maintained an active transcription of histone genes within the cell, and enhanced expression of histone genes in nutritional stress strongly suggest that histones have functional significance in dinoflagellates, although expression at the protein level was below our current detection limits, which suggests a limited role of histones in DNA packaging. Finally, the plausible regulation of histone expression at the gene and protein levels in A. pacificum is discussed.

• Journal of Plant Biotechnology
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• 제36권4호
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• pp.407-414
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• 2009

A cDNA clone encoding phytocystatin was isolated from Brassica rapa seedlings, through rapid amplification of cDNA ends (RACE). This gene (name as Brcpi1; GenBank accession no.: EF079953) had a total length of 881 bp with an open reading frame of 609 bp, and encoded predicted polypeptide of 203 amino acid (aa) residues including a putative N-terminal signal peptide. Other relevant regions found its sequence included the G and PW conserved aa motifs, and the consensus LARFAV sequence for phytocystatins and the reactive site QVVAG. The BrCPI1 protein shared 95, 94, 81, 80 and 78% identity with other CPI proterins isolated from Brassica oleracea (BoCPI-1), Arabidopsis thaliana (AtCY SB), Glycine max (GmCPI), Oryza sativa (OsCYS-2) and Zea may (ZmCPI) at amino acid level, respectively. Southern blot analysis showed that Brcpi1 was a low copy gene. Expression pattern analysis revealed that Brcpi1 was a tissue-specific expressing gene during reproductive growth and strongly expressed at mature seedling stages. Furthermore, overexpression of Brcpi1 in transgenic Arabidopsis was enhanced tolerance to salt and cold stresses. Meanwhile the juvenile seedling of Brcpi1 transgenic plants was not affected by various concentrations ABA in MS medium. Taken together, the results showed that Brcpi1 functioned as a cysteine protease inhibitor and it exhibited a protective agent against diverse types of abiotic stress, which induced this gene in a tissue- and stress-specific manner.

• Molecules and Cells
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• 제37권9호
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• pp.672-684
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• 2014

The exact causes of cell death in Parkinson's disease (PD) remain unknown despite extensive studies on PD.The identification of signaling and metabolic pathways involved in PD might provide insight into the molecular mechanisms underlying PD. The neurotoxin 1-methyl-4-phenylpyridinium ($MPP^+$) induces cellular changes characteristic of PD, and $MPP^+$-based models have been extensively used for PD studies. In this study, pathways that were significantly perturbed in $MPP^+$-treated human neuroblastoma SH-EP cells were identified from genome-wide gene expression data for five time points (1.5, 3, 9, 12, and 24 h) after treatment. The mitogen-activated protein kinase (MAPK) signaling pathway and endoplasmic reticulum (ER) protein processing pathway showed significant perturbation at all time points. Perturbation of each of these pathways resulted in the common outcome of upregulation of DNA-damage-inducible transcript 3 (DDIT3). Genes involved in ER protein processing pathway included ubiquitin ligase complex genes and ER-associated degradation (ERAD)-related genes. Additionally, overexpression of DDIT3 might induce oxidative stress via glutathione depletion as a result of overexpression of CHAC1. This study suggests that upregulation of DDIT3 caused by perturbation of the MAPK signaling pathway and ER protein processing pathway might play a key role in $MPP^+$-induced neuronal cell death. Moreover, the toxicity signal of $MPP^+$ resulting from mitochondrial dysfunction through inhibition of complex I of the electron transport chain might feed back to the mitochondria via ER stress. This positive feedback could contribute to amplification of the death signal induced by $MPP^+$.

• Earthquakes and Structures
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• 제17권3호
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• pp.245-256
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• 2019

An experimental study was conducted to evaluate the dynamic behavior of a free-standing frame equipped with a movable base system using cast iron and mortar as the bearing materials. The preliminary friction test indicated that a graphite layer developed on the interface and exhibited stable friction behavior. The friction coefficient ranged from 0.33 to 0.36 when the applied normal compression stress ranged from 2.6 to 5.2 MPa. The effect of the variation of normal compression stress would be small. Shaking table tests on the free-standing frame showed that rock, slide, and rock-slide responses occurred. The cumulative slide distance reached 381 mm under JMA Kobe wave excitation; however, only a few cyclic slides occurred at the same locations along the moving track. Most surfaces sustained single slides. Similar results can be observed in other shaking conditions. The insufficient cyclic sliding and significant rocking resulted in a few graphite layers on the mortar surfaces. Friction coefficients were generally similar to those obtained in the preliminary friction tests; however, the values fluctuated when the rocking became significant. The collisions due to rocking caused strong horizontal acceleration responses and resulted in high friction coefficient. In addition, the strong horizontal acceleration responses caused by the collisions made the freestanding specimen unable to reduce the input horizontal acceleration notably, even when slippage occurred. Compared with the counterpart fixed-base specimen, the specimen equipped with the iron-mortar base could reduce the horizontal acceleration amplification response and the structural deformation, whereas the vertical acceleration response was doubled due to collisions from rocking.