• Molecules and Cells
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• v.28 no.5
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• pp.417-422
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• 2009

Mitogen activated protein kinase (MAPK) are known to get activated during various stress signals and transduce the message from the cell membrane to the nucleus for appropriate cellular reorganization. Though, a certain basal activity of MAPK is often observed in the control plants. Prolonged exposure of rice plants to lowered or elevated temperature exhibited a rhythm in the activation of MAPKs. We analyzed existence of a possible endogenous rhythm in the activity of MAPKs in rice plants. The plants growing at constant temperature entrained in 16/8 h day-night cycle showed diurnal rhythm in activity. When the activation of MAPK was tested under continuous conditions by shifting plants to continuous darkness for a period of 72 h, the periodic rhythm persisted and followed a circadian pattern. Analysis of the transcripts of group A, B and C members of MAPKs under above conditions by quantitative real time PCR revealed that the members of group C exhibit periodic rhythm. Our data indicates that the MAP kinase activity in rice follows rhythmic expression in a circadian manner.

• Journal of Power Electronics
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• v.18 no.6
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• pp.1819-1829
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• 2018

A three-phase, three-switch, and three-level boost-type PWM rectifier (Vienna rectifier) is proposed as an active front-end power factor correction (PFC) rectifier for telecom loads. The proposed active front-end PFC rectifier system is modeled by the switching cycle average model. The relation between duty ratios and DC link capacitor voltages is derived in terms of the system input currents. Furthermore, the feasible switching states are identified and applied to the proposed system to reduce the switching stress and DC ripples. A detailed equivalent circuit analysis of the proposed front-end PFC rectifier is conducted, and its performance is verified through simulations in MATLAB. Simulation results are verified using an experimental setup of an active front-end PFC rectifier that was developed in the laboratory. Simulation and experimental results demonstrate the improved power quality parameters that are in accordance with the IEEE and IEC standards.

• Journal of Korean Academy of Fundamentals of Nursing
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• v.25 no.3
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• pp.185-196
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• 2018

Purpose: The purpose of this study was to investigate the alopecia experience in adults and to explain the process of their experiences. Methods: Using a grounded theory methodology, 18 interviews were performed with fourteen men and four women, 34~57 years of age, suffering from alopecia. Data were analyzed using the constant comparative analysis method. Results: The core category emerged as "inescapable fetters". adults with alopecia engaged in three stages: embarrassment, seeking solution, and acceptance phase. Causal conditions were a vicious cycle of stress, biological factors and poor life style. Contextual conditions were recognition of irreversibleness, negative social awareness, and marriage. The central phenomenon of the adaptation process among the adults with alopecia was withdrawn life due to negative body image. Action/Interaction strategies included rely on medical treatment, efforts to take good care of hair, research for information treatment, efforts to cover up hair loss, and mind control. Intervening conditions were time cost, economic cost, support of surrounding people. Consequences was burden of unfinished lifetime homework. Conclusion: When caring for these adults, it is important to identify needs, allow patients to express what they want at that moment and support them in maintaining a daily life.

• Journal of Korean Society on Water Environment
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• v.35 no.2
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• pp.131-144
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• 2019

Climate Change affects the hydrological cycle in agricultural watersheds through rising air temperature and changing rainfall patterns. Agricultural watersheds in Korea are characterized by extensive paddy fields and intensive water use, a resource that is under stress from the changing climate. This study analyzed the effects of climate change on river flows for Geum Cheon and Eun-San Choen watershed using STREAM, a semi-distributed watershed model. In order to evaluate the performance and improve the reliability of the model, calibration and validation of the model was done for one flow observation point and three reservoir water storage ratio points. Climate change scenarios were based on RCP data provided by the Korea Meteorological Administration (KMA) and bias corrections were done using the Quantile Mapping method to minimize the uncertainties in the results produced by the climate model to the local scale. Because of water mass-balance, evapotranspiration tended to increase steadily with an increase in air temperature, while the increase in RCP 8.5 scenario resulted in higher RCP 4.5 scenario. The increase in evapotranspiration led to a decrease in the river flow, particularly the decrease in the surface runoff. In the paddy agricultural watershed, irrigation water demand is expected to increase despite an increase in rainfall owing to the high evapotranspiration rates occasioned by climate change.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.21 no.1
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• pp.1-8
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• 2023

The major concern in the deep geological disposal of spent nuclear fuels include sulfide-induced corrosion and stress corrosion cracking of copper canisters. Sulfur diffusion into copper canisters may induce copper embrittlement by causing Cu₂S particle formation along grain boundaries; these sulfide particles can act as crack initiation sites and eventually cause embrittlement. To prevent the formation of Cu₂S along grain boundaries and sulfur-induced copper embrittlement, copper alloys are designed in this study. Alloying elements that can act as chemical anchors to suppress sulfur diffusion and the formation of Cu₂S along grain boundaries are investigated based on the understanding of the microscopic mechanism of sulfur diffusion and Cu₂S precipitation along grain boundaries. Copper alloy ingots are experimentally manufactured to validate the alloying elements. Microstructural analysis using scanning electron microscopy with energy dispersive spectroscopy demonstrates that Cu₂S particles are not formed at grain boundaries but randomly distributed within grains in all the vacuum arc-melted Cu alloys (Cu-Si, Cu-Ag, and Cu-Zr). Further studies will be conducted to evaluate the mechanical and corrosion properties of the developed Cu alloys.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.21 no.3
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• pp.411-417
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• 2023

During the operation of a nuclear power plant (NPP), the generation of radioactive waste, including dry active waste (DAW), concentrates, spent resin, and filters, mandates the implementation of appropriate disposal methods to adhere to Korea's waste acceptance criteria (WAC). In this context, this study investigates the potential use of polymer concrete (PC) as a high-integrity container (HIC) material for solidifying and packaging these waste materials. PC is a versatile composite material comprising binding polymers, aggregates, and additives, known for its exceptional strength and chemical stability. A comprehensive analysis of PC's long-term integrity was conducted in this study. First, its compressive strength, which is crucial for ensuring the structural stability of HICs over extended periods, was evaluated. Subsequently, the resilience of PC was tested under various stress conditions, including biological, radiological, thermal, and chemical stressors. The findings of this study indicate that PC exhibits remarkable long-term properties, demonstrating exceptional stability even when subjected to diverse stressors. The results therefore underscore the potential viability of PC as a reliable material for constructing high-integrity containers, thus contributing to the safe and sustainable management of radioactive waste in NPPs.

• BMB Reports
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• v.57 no.6
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• pp.299-304
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• 2024

Upregulation of PRAME (preferentially expressed antigen of melanoma) has been implicated in the progression of a variety of cancers, including melanoma. The tumor suppressor p53 is a transcriptional regulator that mediates cell cycle arrest and apoptosis in response to stress signals. Here, we report that PRAME is a novel repressive target of p53. This was supported by analysis of melanoma cell lines carrying wild-type p53 and human melanoma databases. mRNA expression of PRAME was downregulated by p53 overexpression and activation using DNA-damaging agents, but upregulated by p53 depletion. We identified a p53-responsive element (p53RE) in the promoter region of PRAME. Luciferase and ChIP assays showed that p53 represses the transcriptional activity of the PRAME promoter and is recruited to the p53RE together with HDAC1 upon etoposide treatment. The functional significance of p53 activation-mediated PRAME downregulation was demonstrated by measuring colony formation and p27 expression in melanoma cells. These data suggest that p53 activation, which leads to PRAME downregulation, could be a therapeutic strategy in melanoma cells.

• Geomechanics and Engineering
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• v.25 no.3
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• pp.233-243
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• 2021

Rib spalling is a major issue affecting the safety of steeply inclined coal seam. And the failure coal face and support system can be affected with each other to generate a vicious cycle along with inducing large-scale collapse of surrounding rock in steeply inclined coal seam. In order to analyze failure mechanism and propose the corresponding prominent control measures of steeply inclined coal working face, mechanical model based on coal face-support-roof system and mechanical model of coal face failure was established to reveal the disaster mechanism of rib spalling and the sensitive analysis of related factors was performed. Furthermore, taking 3402 working face of Chen-man-zhuang coal mine as engineering background, numerical model by using FLAC3D was built to illustrate the propagation of displacement and stress fields in steeply inclined coal seam and verify the theory analysis as mentioned in this study. The results show that the coal face slide body in steeply inclined working face can be observed as the failure height of upper layer smaller than that of lower layer exhibiting with an irregular quadrilateral pyramid shape. Moreover, the cracks were originated from the upper layer of sliding body and gradually developed to the lower layer causing the final rib spalling. The influence factors on the stability of coal face can be ranked as overlying strata pressure (P) > mechanical parameters of coal body (e.g., cohesion (c), internal fraction angle (φ)) > support strength (F) > the support force of protecting piece (F') > the false angle of working face (Θ). Moreover, the corresponding control measures to maintain the stability of the coal face in the steeply inclined working face were proposed.

• The Korean Journal of Elementary Counseling
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• v.11 no.3
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• pp.427-446
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• 2012

The purpose of this study was to investigate the influence of stress in families of children with disabilities upon a problem-solving ability based on a buffering effect of family resilience through life cycle. To achieve this purpose, the subjects in this study was 546 parents who have kindergarten, elementary, middle & high school students in special schools. The date for this study were derived from questionaries and the answer sheet was analyzed by SPSS(VER. 14). A descriptive statistics, correlation analysis, hierarchical moderating regression analysis were used to investigate the general information and the difference of perception between groups. The findings of this study were as follows: Higher belief system, higher organization type, higher communication process of the family with disabled children tend to appear more positive influences on buffering effects for stresses of family and solving-problem abilities. Findings from the current study demonstrate that the buffering effect of family resilience indicate to have positive influences on the relationship between the stress and the problem-solving ability in the family with disabled children.

• Journal of Microbiology and Biotechnology
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• v.30 no.9
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• pp.1420-1429
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• 2020

Corynebacterium glutamicum, an important industrial strain, has a relatively slower reproduction rate. To acquire a growth-boosted C. glutamicum, a descendant strain was isolated from a continuous culture after 600 generations. The isolated descendant C. glutamicum, JH41 strain, was able to double 58% faster (t_d=1.15 h) than the parental type strain (PT, t_d=1.82 h). To understand the factors boosting reproduction, the transcriptomes of JH41 and PT strains were compared. The mRNAs involved in respiration and TCA cycle were upregulated. The intracellular ATP of the JH41 strain was 50% greater than the PT strain. The upregulation of NCgl1610 operon (a putative dyp-type heme peroxidase, a putative copper chaperone, and a putative copper importer) that presumed to role in the assembly and redox control of cytochrome c oxidase was found in the JH41 transcriptome. Plasmid-driven expression of the operon enabled the PT strain to double 19% faster (t_d=1.82 h) than its control (t_d=2.17 h) with 14% greater activity of cytochrome c oxidase and 27% greater intracellular ATP under the oxidative stress conditions. Upregulations of genes those might enhance translation fitness were also found in the JH41 transcriptome. Plasmid-driven expressions of NCgl0171 (encoding a cold-shock protein) and NCgl2435 (encoding a putative peptidyl-tRNA hydrolase) enabled the PT to double 22% and 32% faster than its control, respectively (empty vector: t_d=1.93 h, CspA: t_d=1.58 h, and Pth: t_d=1.44 h). Based on the results, the factors boosting growth rate in C. gluctamicum were further discussed in the viewpoints of cellular energy state, oxidative stress management, and translation.