• Molecules and Cells
• /
• v.41 no.5
• /
• pp.413-422
• /
• 2018

Soybean transgenic plants with ectopically expressed AtABF3 were produced by Agrobacterium-mediated transformation and investigated the effects of AtABF3 expression on drought and salt tolerance. Stable Agrobacterium-mediated soybean transformation was carried based on the half-seed method (Paz et al. 2006). The integration of the transgene was confirmed from the genomic DNA of transformed soybean plants using PCR and the copy number of transgene was determined by Southern blotting using leaf samples from $T_2$ seedlings. In addition to genomic integration, the expression of the transgenes was analyzed by RT-PCR and most of the transgenic lines expressed the transgenes introduced. The chosen two transgenic lines (line #2 and #9) for further experiment showed the substantial drought stress tolerance by surviving even at the end of the 20-day of drought treatment. And the positive relationship between the levels of AtABF3 gene expression and drought-tolerance was confirmed by qRT-PCR and drought tolerance test. The stronger drought tolerance of transgenic lines seemed to be resulted from physiological changes. Transgenic lines #2 and #9 showed ion leakage at a significantly lower level (P < 0.01) than ${\underline{n}}on-{\underline{t}}ransgenic$ (NT) control. In addition, the chlorophyll contents of the leaves of transgenic lines were significantly higher (P < 0.01). The results indicated that their enhanced drought tolerance was due to the prevention of cell membrane damage and maintenance of chlorophyll content. Water loss by transpiration also slowly proceeded in transgenic plants. In microscopic observation, higher stomata closure was confirmed in transgenic lines. Especially, line #9 had 56% of completely closed stomata whereas only 16% were completely open. In subsequent salt tolerance test, the apparently enhanced salt tolerance of transgenic lines was measured in ion leakage rate and chlorophyll contents. Finally, the agronomic characteristics of ectopically expressed AtABF3 transgenic plants ($T_2$) compared to NT plants under regular watering (every 4 days) or low rate of watering condition (every 10 days) was investigated. When watered regularly, the plant height of drought-tolerant line (#9) was shorter than NT plants. However, under the drought condition, total seed weight of line #9 was significantly higher than in NT plants (P < 0.01). Moreover, the pods of NT plants showed severe withering, and most of the pods failed to set normal seeds. All the evidences in the study clearly suggested that overexpression of the AtABF3 gene conferred drought and salt tolerance in major crop soybean, especially under the growth condition of low watering.

• Journal of the Korean Geotechnical Society
• /
• v.18 no.5
• /
• pp.37-42
• /
• 2002

Artificial neural networks are efficient computing techniques that are widely used to solve complex problems in many fields. In this paper a liquefaction potential was estimated by using a back propagation neural network model applicated to cyclic triaxial test data, soil parameters and site investigation data. Training and testing of the network were based on a database of 43 cyclic triaxial test data from 00 sites. The neural networks are trained by modifying the weights of the neurons in response to the errors between the actual output values and the target output value. Training was done iteratively until the average sum squared errors over all the training patterns were minimized. This generally occurred after about 15,000 cycles of training. The accuracy from 72% to 98% was shown for the model equipped with two hidden layers and ten input variables. Important effective input variables have been identified as the NOC,$D_10$ and (N$_1$)$_60$. The study showed that the neural network model predicted a CSR(Cyclic shear stress Ratio) of silty-sand reasonably well. Analyzed results indicate that the neural-network model is more reliable than simplified method using N value of SPT.

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.20 no.2
• /
• pp.122-130
• /
• 2016

The past few decades of industrialization enabled human-centered stream developments, which in turn resulted in constructing straight or covered streams, which are used only for sewage disposal purpose. However, these types of streams have become the cause of flood damages such as localized heavy rain. In response, various construction methods have been implemented to prevent stream and embankment damages. However, regulations regarding these measures only lay out minimum standards such as the height of slopes and the minimum angle of inclination. Moreover, examination of tractive force, the most crucial factor in preventing flood damage, is nonexistent. Therefore, this study evaluates various tractive forces by implementing a porous concrete tetrapod at a full scale artificial stream for experiment, controlling the rate of inflow, and measuring the velocity and depth of the stream under different experiment conditions. The test results of the compressive strength, and porosity and density of rock of the porous concrete tetrapod was between 16.6 and 23.2 MPa, and the actual measurement of air void was 10.1%, thus satisfying domestic standard. The result of tractive force experiment showed a limiting tractive force of $47.202N/m^2$, not satisfying the tractive force scope of $67N/m^2$ the stream design working expertise proposes. However, there was neither damage nor loss of blocks and hardpan. Based on previous researches, it can be expected that there will be resistance against a stronger tractive force. Therefore, it is necessary to conduct another experiment on practical limiting tractive force by adjusting some experimental conditions.

• Tunnel and Underground Space
• /
• v.30 no.2
• /
• pp.109-135
• /
• 2020

Globally, the deepening depth in the underground is a situation of the high interest for a purpose of the development of various facilities. The development of deep underground space should be based on the structural stability of rocks. Spalling is known to have an impact on the structural stability degradation in deep underground space. As an attempt to predict spalling, many researchers have proposed predicted conditions in accordance with stress states which occur around the tunnel, rock conditions, and types of rock. In addition, the analysis on spalling method has been verified by using computer modeling such as FLAC, EXAMINE, Insight 2D, UDEC and FRACOD, along with in-situ measurement results. In Canada URL (Underground Research Tunnel), CWFS model (Cohesion Weakening Frictional Strengthening) was used to precisely predict for the state of spalling, comparing spalling modeling. CWFS model has been identified as a reliable method for predicting such phenomena. This study aims to analyze several cases of spalling, and then make a comparison between the conditions for spalling occurrence and the predicted results of model CWFS. With this, it investigates the applicability of prediction of spalling, targeting pillar under deep depth condition.

• Journal of Korean Society of Steel Construction
• /
• v.23 no.2
• /
• pp.199-210
• /
• 2011

The cables in cable-stayed bridges are under high stress and are very sensitive to vibration due to their small section areas compared with other members. Therefore, it is reasonable to evaluate the cable impact factor by taking into account the dynamic effect due to moving-vehicle motion. In this study, the cable impact factors were evaluated via moving-vehicle-load analysis, considering the design parameters, i.e., vehicle weight, cable model, road surface roughness, vehicle speed, longitudinal distance between vehicles. For this purpose, two steel composite cable-stayed bridges with 230- and 540-m main spans were selected. The results of the analysis were then compared with those of the influence line method that is currently being used in design practice. The road surface roughness was randomly generated based on ISO 8608, and the convergence of impact factors according to the number of generated road surfaces was evaluated to improve the reliability of the results. A9-d.o.f. tractor-trailer vehicle was used, and the vehicle motion was derived from Lagrange's equation. 3D finite element models for the selected cable-stayed bridges were constructed with truss elements having equivalent moduli for the cables, and with beam elements for the girders and the pylons. The direct integration method was used for the analysis of the bridge-vehicle interaction, and the analysis was conducted iteratively until the displacement error rate of the bridge was within the specified tolerance. It was acknowledged that the influence line method, which cannot consider the dynamic effect due to moving-vehicle motion, could underestimate the impact factors of the end-cables at the side spans, unlike moving-vehicle-load analysis.

• Asian Spine Journal
• /
• v.12 no.6
• /
• pp.1060-1068
• /
• 2018

Study Design: Retrospective study. Purpose: Lumbar intervertebral disc degeneration is an important cause of low back pain. Overview of Literature: Spinal fusion is often reported to have a good course for adolescent idiopathic scoliosis (AIS). However, many studies have reported that adjacent segment degeneration is accelerated after lumbar spinal fusion. Radiography is a simple method used to evaluate the orientation of the vertebral column. magnetic resonance imaging (MRI) is the method most often used to specifically evaluate intervertebral disc degeneration. The Pfirrmann classification is a well-known method used to evaluate degenerative lumbar disease. After spinal fusion, an increase in stress, excess mobility, increased intra-disc pressure, and posterior displacement of the axis of motion have been observed in the adjacent segments. Methods: we retrospectively secured and analyzed the data of 15 patients (four boys and 11 girls) with AIS who underwent a spinal fusion surgery. We studied the full-length view of the spine (anterior-posterior and lateral) from the X-ray and MRI obtained from all patients before surgery. Postoperatively, another full-length spine X-ray and lumbosacral MRI were obtained from all participants. Then, pelvic tilt, sacral slope, curve correction, and fused and free segments before and after surgery were calculated based on X-ray studies. MRI images were used to estimate the degree to which intervertebral discs were degenerated using Pfirrmann grading system. Pfirrmann grade before and after surgery were compared with Wilcoxon signed rank test. While analyzing the contribution of potential risk factors for the post-spinal fusion Pfirrmann grade of disc degeneration, we used generalized linear models with robust standard error estimates to account for intraclass correlation that may have been present between discs of the same patient. Results: The mean age of the participant was 14 years, and the mean curvature before and after surgery were 67.8 and 23.8, respectively (p<0.05). During the median follow-up of 5 years, the mean degree of the disc degeneration significantly increased in all patients after surgery (p<0.05) with a Pfirrmann grade of 1 and 2.8 in the L2-L3 before and after surgery, respectively. The corresponding figures at L3-L4, L4-L5, and L5-S1 levels were 1.28 and 2.43, 1.07 and 2.35, and 1 and 2.33, respectively. The lower was the number of free discs below the fusion level, the higher was the Pfirrmann grade of degeneration (p<0.001). Conversely, the higher was the number of the discs fused together, the higher was the Pfirrmann grade. Conclusions: we observed that the disc degeneration aggravated after spinal fusion for scoliosis. While the degree of degeneration as measured by Pfirrmann grade was directly correlated by the number of fused segments, it was negatively correlated with the number of discs that remained free below the lowermost level of the fusion.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.22 no.2
• /
• pp.169-178
• /
• 2021

This study is a qualitative study to explore the experiences of nursing students' psychological nursing practice in-school. The subjects of this study were 62 students who were the 3rd grade nursing students at a University in Gangwon-do. The data were collected from August 3rd to August 20th, 2020. For data analysis, Colaizzi's qualitative analysis method was applied. Six categories were derived from data analysis: 1) Lingering affection for clinical practice in field. 2) Achievement through learning 3) Fear of nurse's role in future 4) Burden for many tasks 5) Feeling free of stress from clinical practice in field 6) Importance of cooperation with other departments. The positive aspects of in-school practice education, which from nursing students' evaluation, were immediate feedback, detailed explanations, and indirect experiences. Based on this study result, it is recommended to develop in-school nursing practice education program for gap-reducing with clinical practice. It would be consisted of various contents: simulation, online/non-face-to-face practice, face-to-face practice for indirect experience. In addition, these multi-aspect effort is needed more in psychological nursing practice education to reduce the gap with clinical practice such as therapeutic communication and hallucination interventions.

• Korean Journal of Remote Sensing
• /
• v.37 no.3
• /
• pp.637-648
• /
• 2021

In order to support the establishment of a farming plan, it is important to preemptively evaluate crop changes and to provide precise information. Therefore, it is necessary to provide customized information suitable for decision-making by farming stage through scientific and continuous monitoring using drones. This study was carried out to support the establishment of the farming plan for ground vegetable. The cultivation management map of each information was obtained from preliminary study. Three cultivation management maps include 'field emergence map', 'stress map' and 'productivity map' reflected spatial variation in the plantation by providing information in units of plants based on 3-dimensions. Application fields of the cultivation management map can be summarized as follows: detect miss-planted, replanting decision, fertilization, weeding, pest control, irrigation schedule, market quality evaluation, harvest schedule, etc.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.22 no.1
• /
• pp.116-122
• /
• 2021

This study quantitatively evaluated the performance improvement of a circular reinforced concrete pier under dynamic load with strengthening using a steel plate. Various three-dimensional elements were applied using the finite element program ABAQUS. The analytical parameters included the ratios of the steel cover length to the pier's total height and the ratios of the steel cover thickness to the pier diameter for inelastic-nonlinear analysis. The lower part of the pier had fixed boundary conditions, and lateral repetitive loads were applied at the top of the pier. The pier was investigated to evaluate the dynamic performance based on the load-displacement curve, stress-strain curve, ductility, energy absorption capability, and energy ratio. The yield and ultimate loads of piers with steel covers increased by 3.76 times, and the energy absorption capability increased by 4 times due to the confinement effects caused by the steel plate. A plastic hinge part of the column with a steel plate improved the ductility, and the thicker the steel plate was, the greater the energy absorption capacity. This study shows that the reinforced pier should be improved in terms of the seismic performance.

• The Journal of the Korea Contents Association
• /
• v.20 no.12
• /
• pp.393-405
• /
• 2020

The purpose of this study is to develop and validate a scale of vicarious trauma for sexual violence counselors. To this end, the concept of composition and preliminary questions of the vicarious trauma of sexual violence counselors were selected through the analysis of a content of individual interviews of sexual violence counselors and the precedent theory. Then, preliminary scale was developed after expert's evaluation of the validity of the contents. Next, as a result of a confirmatory factor analysis on the preliminary scale of 349 sexual violence counselors with experience in consulting sexual violence clients, it is confirmed that the conformity of five-factor structure was found to be favorable. As a result of convergent and discriminative validity analysis, it was confirmed that the developed scale is a reasonable tool for measuring vicarious trauma of sexual violence counselors by showing proper correlation with Secondary Traumatic Stress Scale, Counselor Burnout Inventory and Inventory of Countertransference Behavior. Furthermore, as a result of correlation analysis between professional identity and Belief in a Just World, it confirmed that it has predictive validity of the scale by indicating a high negative correlation. Furthermore, the relationship between the scale of vicarious trauma of sexual violence counselors and career background was analyzed. Finally, based on the results of this study, the significance and limitations of this study and the direction of future research were discussed.