• 한국산업융합학회 논문집
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• 제2권1호
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• pp.77-83
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• 1999

Grown in the secondary broth of production media, the strain Streptomyces albulus has increased more the production of its metabolite ${\varepsilon}$-poly-L-lysine, one of poly(amino acid)s used as disinfecting food additives, than the strain in the primary culture of growth nutrients. Having the strain removed, the large concentrate obtained by ultrafiltrating the secondary culture broth. The concentrated production broth exchanged into followed by detecting in UV flowcell at 220nm the peptide bond of the components eluting the adsorbed proteins and polylysine with NaCl salt of gradient concentration, and has separated into five components. Among them the component in the fourth peak fraction has proved to be the pure ${\varepsilon}$-poly-L-lysine after the portion being hydrolyzed the fraction with HCl into amino acid followed by being the composing amino acid analysis.

• 한국산업융합학회 논문집
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• 제2권1호
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• pp.91-95
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• 1999

The Streptomyces albulus broth, when the polylysine in the broth, that has powerful growth inhibiting effect far many microbes, is its maximum, had filtered off the cells, to use the broth as preservative for keeping favorable taste of Korean Kimchi. Some microorganisms in their specific growth medium, known to deteriorate the useful nutrient of the Kimchi, has grown with different amounts of the inhibiting broth, to determine the minimum growth inhibition concentration. The ${\varepsilon}$-poly-L-lysine had been identified from the IR spectroscopic analysis of the purified poly lysine of the broth from ion exchange chromatographic separation. The content of the polylysine had been determined by methyl orange decoloration effect. Though the minimum inhibition concentration, evaluated by the naked eye based on the conventional method measuring the turbid feature after 18 hours of culture, has different values each other, the observed effects confirmed that the crude broth could be used as a natural preservative for the Kimchi in extending the fair taste.

• Journal of Microbiology and Biotechnology
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• 제25권11호
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• pp.1819-1826
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• 2015

Poly(ε-ʟ-lysine) (ε-PL) is a novel bioactive polymer secreted by filamentous bacteria. Owing to lack of a genetic system for most ε-PL-producing strains, very little research on enhancing ε-PL biosynthesis by genetic manipulation has been reported. In this study, an effective genetic system was established via intergeneric conjugal transfer for Streptomyces albulus PD-1, a famous ε-PL-producing strain. Using the established genetic system, the Vitreoscilla hemoglobin (VHb) gene was integrated into the chromosome of S. albulus PD-1 to alleviate oxygen limitation and to enhance the biosynthesis of ε-PL in submerged fermentation. Ultimately, the production of ε-PL increased from 22.7 g/l to 34.2 g/l after fed-batch culture in a 5 L bioreactor. Determination of the oxygen uptake rate, transcriptional level of ε-PL synthetase gene, and ATP level unveiled that the expression of VHb in S. albulus PD-1 enhanced ε-PL biosynthesis by improving respiration and ATP supply. To the best of our knowledge, this is the first report on enhancing ε-PL production by chromosomal integration of the VHb gene in an ε-PL-producing strain, and it will open a new avenue for ε-PL production.

• 한국미생물·생명공학회지
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• 제6권4호
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• pp.187-196
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• 1978

미생물이 하는 생산하는 대사길항물질을 screening 한 결과 최소검정배지상에서 Gram 양성균과 Gram 음성균에 다같이 강한 항균작용을 나타내고 L-aspar tic acid와 L-g1utamic acid에 의하여 길항당하는 물질 N-2292를 생산하는 방선균을 분리하였다. 본 생산균을 분류동정한 결과, Streptomyces albulus 로 보거나 혹은 그 유연균으로 동정하였다. N-2292물질의 배양조건을 검토하여 최적조건에서 대량 배양하고 주로 ion 교환수지, Dowex 50을 사용하여 활성물질을 분리정제하였으며 배양액 약 10ι에서 약 40mg의 백색분말을 얻었다. 본물질은 그 의 이화학적 성질로 보아 indole 화합물을 함유하는 peptide 양 물질임을 추정할 수가 있었다. 생물학적 성질은 최소검정배지상에서 Gram 양성 및 Gram 음성세균에 항균력을 나타냈다. 이러한 항균력은 L-Asp. 및 L-Glu.의 첨가에 의해서 조해당하였다.

• 미생물학회지
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• 제14권1호
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• pp.25-35
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• 1976

A taxonomical sutdy was made on the Streptomyces species isolated from soils in this country, most of which were collected during the period from April, 1974 to July 1975. J.S.P. Methods (1966), I.S.P. Descriptions (1968-1972) and Bergy's Manual of Determinative Bacteriology (1974) were used for most of the experimental methods and identifications. As a result, 24 species were identified as follows ; S. albolongus, S galticri.S. Nashvillensis. S. showdoensis. S.norbonensis. S. flacocirens. S. resistomycificus. S> reshiriensis. S. chromofuscus. S. parvullus. S, chibaensis. S.canus. S. albulus. S. amlachiticus. S.griseoflavus. S. griscoincarnotus. .S. rubiginosus. S. bacillaris. S. setonill. S.intermedius. S. griseinus. S.subrutilus. S.reseosporus.