• Title/Summary/Keyword: Streptomyces albulus

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Separation of ε-poly-L-lysine from the fermentation broth of Streptomyces albulus (Streptomyces albulus 배양액으로부터 ε-poly-L-lysine의 분리)

  • Sun, Heung-Suk;Park, Chan-Young
    • Journal of the Korean Society of Industry Convergence
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    • v.2 no.1
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    • pp.77-83
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    • 1999
  • Grown in the secondary broth of production media, the strain Streptomyces albulus has increased more the production of its metabolite ${\varepsilon}$-poly-L-lysine, one of poly(amino acid)s used as disinfecting food additives, than the strain in the primary culture of growth nutrients. Having the strain removed, the large concentrate obtained by ultrafiltrating the secondary culture broth. The concentrated production broth exchanged into followed by detecting in UV flowcell at 220nm the peptide bond of the components eluting the adsorbed proteins and polylysine with NaCl salt of gradient concentration, and has separated into five components. Among them the component in the fourth peak fraction has proved to be the pure ${\varepsilon}$-poly-L-lysine after the portion being hydrolyzed the fraction with HCl into amino acid followed by being the composing amino acid analysis.

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Effects of Crude ε-poly-L-lysine in Streptomyces albulus Broth on Suppression of Microbial Growth in Korean Kimchi (폴리리신을 함유한 Streptomyces albulus 배양액의 김치미생물 성장억제 효과)

  • Kim, Kwang-sub;Lee, Garpee;Sun, Heung-suk;Ahn, Chi-min;Park, Chanyoung
    • Journal of the Korean Society of Industry Convergence
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    • v.2 no.1
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    • pp.91-95
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    • 1999
  • The Streptomyces albulus broth, when the polylysine in the broth, that has powerful growth inhibiting effect far many microbes, is its maximum, had filtered off the cells, to use the broth as preservative for keeping favorable taste of Korean Kimchi. Some microorganisms in their specific growth medium, known to deteriorate the useful nutrient of the Kimchi, has grown with different amounts of the inhibiting broth, to determine the minimum growth inhibition concentration. The ${\varepsilon}$-poly-L-lysine had been identified from the IR spectroscopic analysis of the purified poly lysine of the broth from ion exchange chromatographic separation. The content of the polylysine had been determined by methyl orange decoloration effect. Though the minimum inhibition concentration, evaluated by the naked eye based on the conventional method measuring the turbid feature after 18 hours of culture, has different values each other, the observed effects confirmed that the crude broth could be used as a natural preservative for the Kimchi in extending the fair taste.

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Construction of a Genetic System for Streptomyces albulus PD-1 and Improving Poly(ε-ʟ-lysine) Production Through Expression of Vitreoscilla Hemoglobin

  • Xu, Zhaoxian;Cao, Changhong;Sun, Zhuzhen;Li, Sha;Xu, Zheng;Feng, Xiaohai;Xu, Hong
    • Journal of Microbiology and Biotechnology
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    • v.25 no.11
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    • pp.1819-1826
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    • 2015
  • Poly(ε-ʟ-lysine) (ε-PL) is a novel bioactive polymer secreted by filamentous bacteria. Owing to lack of a genetic system for most ε-PL-producing strains, very little research on enhancing ε-PL biosynthesis by genetic manipulation has been reported. In this study, an effective genetic system was established via intergeneric conjugal transfer for Streptomyces albulus PD-1, a famous ε-PL-producing strain. Using the established genetic system, the Vitreoscilla hemoglobin (VHb) gene was integrated into the chromosome of S. albulus PD-1 to alleviate oxygen limitation and to enhance the biosynthesis of ε-PL in submerged fermentation. Ultimately, the production of ε-PL increased from 22.7 g/l to 34.2 g/l after fed-batch culture in a 5 L bioreactor. Determination of the oxygen uptake rate, transcriptional level of ε-PL synthetase gene, and ATP level unveiled that the expression of VHb in S. albulus PD-1 enhanced ε-PL biosynthesis by improving respiration and ATP supply. To the best of our knowledge, this is the first report on enhancing ε-PL production by chromosomal integration of the VHb gene in an ε-PL-producing strain, and it will open a new avenue for ε-PL production.

Studies on the New Antimetabolites Produced by Microorganisms (미생물이 생산하는 새로운 대사길항물질에 관한 연구)

  • 박부길
    • Microbiology and Biotechnology Letters
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    • v.6 no.4
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    • pp.187-196
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    • 1978
  • Antimetabolite N-2292 substance, an antagonist of L-aspartic acid and L-glutamic acid was isolated from the fermentation broth of Streptomyces. Taxonomical study on the producing strain made it a related species of Streptomyces albulus judged by cultural characteristics and carbon utilization. N-2292 substance was isolated as amorphous white powder with melting point at 185$^{\circ}C$. From the physicochemical characteristics of the substance, it was peptide like substance. It was active against Gram positive and Gram negative bacteria but negative against yeast and mold in its biological properties. It was reversed by L-Asp and L-Glu on the synthetic medium.

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Studies on the Isolation and Identification of Genus Strptomyces (Streptomyces속의 분리 및 동정에 관한 연구)

  • 이민재;하영칠;안정선
    • Korean Journal of Microbiology
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    • v.14 no.1
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    • pp.25-35
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    • 1976
  • A taxonomical sutdy was made on the Streptomyces species isolated from soils in this country, most of which were collected during the period from April, 1974 to July 1975. J.S.P. Methods (1966), I.S.P. Descriptions (1968-1972) and Bergy's Manual of Determinative Bacteriology (1974) were used for most of the experimental methods and identifications. As a result, 24 species were identified as follows ; S. albolongus, S galticri.S. Nashvillensis. S. showdoensis. S.norbonensis. S. flacocirens. S. resistomycificus. S> reshiriensis. S. chromofuscus. S. parvullus. S, chibaensis. S.canus. S. albulus. S. amlachiticus. S.griseoflavus. S. griscoincarnotus. .S. rubiginosus. S. bacillaris. S. setonill. S.intermedius. S. griseinus. S.subrutilus. S.reseosporus.

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