• Mycobiology
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• 제43권3호
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• pp.354-359
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• 2015

Blossom blight in strawberry was first observed in a green house in Nonsan, Damyang, and Geochang areas of Korea, between early January to April of 2012. Disease symptoms started as a grey fungus formed on the stigma, which led to the blossom blight and eventually to black rot and necrosis of the entire flower. We isolated the fungi purely from the infected pistils and maintained them on potato dextrose agar (PDA) slants. To test Koch's postulates, we inoculated the fungi and found that all of the isolates caused disease symptoms in the flower of strawberry cultivars (Seolhyang, Maehyang, and Kumhyang). The isolates on PDA had a velvet-like appearance, and their color ranged between olivaceous-brown and smoky-grey to olive and almost black. The intercalary conidia of the isolates were elliptical to limoniform, with sizes ranging from $5.0{\sim}10.5{\times}2.5{\sim}3.0{\mu}m$ to $4.0{\sim}7.5{\times}2.0{\sim}3.0{\mu}m$, respectively. The secondary ramoconidia of these isolates were 0- or 1-septate, with sizes ranging betweem $10.0{\sim}15.0{\times}2.5{\sim}3.7{\mu}m$ and $8.7{\sim}11.2{\times}2.5{\sim}3.2{\mu}m$, respectively. A combined sequence analysis of the internal transcribed spacer regions, partial actin (ACT), and translation elongation factor 1-alpha (TEF) genes revealed that the strawberry isolates belonged to two groups of authentic strains, Cladosporium cladosporioides and C. tenuissimum. Based on these results, we identified the pathogens causing blossom blight in strawberries in Korea as being C. cladosporioides and C. tenuissimum.

• 식물병연구
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• 제11권1호
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• pp.35-38
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• 2005

2001년에서 2003년까지 국내 딸기주산단지에서 딸기시들음병 발생실태 조사를 실시하였다. 딸기시들음병 발생은 주로 육묘기인 6월부터 8월, 정식기인 9월부터 10월, 수확기인 1월부터 3월까지 발생되었다. 병징은 뿌리가 갈변되어 크라운의 도관을 따라 갈변되었고 짝잎을 형성하였다. 시들음병 발생은 214개 포장 중 37개 포장에서 최대 30%까지 발생되었다. 딸기 시들음병은 Dochiodome, 매향, Redpearl, Samaberry, Akihime 품종 등 조사된 모든 품종에서 발생하였으나, 특히 Samaberry, 매향, Dochiodome 에서 많이 발생하였다. 시들음병 발생토양은 무발병 토양보다 전기전도도, 질소, 인산농도가 높았고, pH는 낮게 나타났다. Dochiodome 와 Samaberry 품종에서 분리된 Fo47, Fo79 균주는 공시된 4품종에 대해 병원성이 강했으며 특히 Dochiodome, Redpearl, 매향 품종은 더 감수성이었다.

• The Plant Pathology Journal
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• 제21권1호
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• pp.59-63
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• 2005

Bacillus subtilis S1-0210 was selected as a biological agent against Botrytis cinerea in strawberry. The isolate inhibited mycelial growth of B. cinerea in vitro tests. A wettable powder formulation of B. subtilis S1-0210 significantly reduced infection rates with lower than 5%, compared with higher than 70% of infection rates in untreated control. The formulation showed 85 to 89% control efficacies of gray mold incidences on fruits of strawberry in pots. Pre-treatment of the agent was more effective in controlling gray mold on fruits and leaves than post-treatment at the early stage of disease development. The formulation also showed 70% control efficacy of gray mold incidence on fruits of strawberry in a field trial. The results indicate that B. subtilis S1-0210 in the wettable powder formulation may be a potential biocontrol agent to control gray mold on strawberry.

• 식물병연구
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• 제8권1호
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• pp.50-54
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• 2002

딸기탄저병의 방제방법을 개발하기 위해 배지에서 약제별 균사생장억제정도를 검정한 결과 Tricylazole수화제가 가장 좋은 효과를 보여 주었으나 딸기잎에 접종하여 효과를 검정하였을 때는 Azoxystrobin 수화제가 가장 좋은 효과를 보여주었다. 포장에서 방제효과는 관주 및 엽면살포는 방제효과가 없었으나 Azoxystrobin 수화제 1,000배액에 10분간 침지시 우수한 방제효과를 보여 주었고 처리시간과 처리농도간 약효의 차이는 볼 수 없었다.

• 식물병과 농업
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• 제1권2호
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• pp.1-8
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• 1995

• Food Science and Biotechnology
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• 제16권1호
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• pp.123-126
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• 2007

The quality of Yukbo strawberry (Fragaria ananassa Duch.) fruit declines rapidly after harvest. Therefore, we examined the effects of nitric oxide (NO) on its respiration rate, quality, and shelf life. Strawberries were fumigated for 5 hr at 0, 50, 100, 200, or $500\;{\mu}L/L$ NO atmosphers, followed by a hold at $18^{\circ}C$ in air. Treatment with NO delayed the onset of ethylene production ad reduced respiration, which at $18^{\circ}C$ resulted I a maintained quality and prolonged shelf life. The NO-treated strawberries were also firmer and had a lower incidence of disease than the untreated fruit. The effect of NO on fruit quality was dose-dependent. Strawberries that were treated with low and high concentrations of 50 and $500\;{\mu}L/L$ No, respectively, had severe disease incidence and were of poor quality. Treating with NO at a concentration of $200\;{\mu}L/L$ appeared to slow down the ripening and senescence of fruit stored at $18^{\circ}C$. Calyx browning, respiration, and rot development progressed more quickly in strawberries treated with $500\;{\mu}L/L$ NO compared to those treated with $200\;{\mu}L/L$ No.

• Mycobiology
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• 제50권1호
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• pp.79-85
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• 2022

Strawberries are a popular economic crop, and one of the major plantations and exporting countries is Korea in the world. The Fusarium oxysporum species complex (FOSC) is a soil-borne pathogen with genetic diversity, resulting in wilt disease in various crops. In Korea, strawberries wilt disease was first reported in the 1980s due to the infection of FOSC, causing significant economic damage every year. The causal agent, F. oxysporum f. sp. fragariae, is a soil-borne pathogen with a characteristic of FOSC that is difficult to control chemically and mutates easily. This study obtained genetic polymorphism information that was based on AFLP, of F. oxysporum f. sp. fragariae 91 strains, which were isolated from strawberry cultivation sites in Gyeongsangnam-do and Chungcheongnam-do, and compared strains information, which was the isolated location, host variety, response to chemical fungicide, and antagonistic bacteria, and mycelium phenotype. As a result, AFLP phylogeny found that two groups were mainly present, and group B was present at a high frequency in Gyeongsangnam-do. Group B proved less sensitive to tebuconazole than group A through Student's t-test. In addition, the fractions pattern of AFLP was calculated by comparing the strain information using PCA and PERMANOVA, and the main criteria were separated localization and strawberry varieties (PERMANOVA; p< 0.05). And tebuconazole was different with weak confidence (PERMANOVA; p< 0.10). This study suggests that the F. oxysporum f. sp. fragariae should be continuously monitored and managed, including group B, which is less chemically effective.

• 식물병연구
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• 제23권2호
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• pp.186-192
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• 2017

2016년 6월부터 9월까지 국내 딸기 육묘장 117개 포장의 토양으로부터 식물기생선충의 토양 내 감염현황을 조사하였다. 그 결과 뿌리썩이선충(Pratylenchus)과 뿌리혹선충(Meloidogyne)이 11%와 3%로 검출되어 딸기 육묘장의 문제선충으로 나타났다. 육묘 형태별로는 토경육묘에서 뿌리썩이선충과 뿌리혹선충의 발생빈도가 다른 육묘방법에 비해 높았고, 상토를 이용한 비가림 포트육묘에서는 식물기생선충이 검출되지 않았다. 뿌리썩이선충은 딸기뿌리썩이선충(P. penetrans)과 사과뿌리썩이선충(P. vulnus) 2종이 확인 되었으며, 뿌리혹선충은 당근뿌리혹선충(M. hapla) 1종이 확인되었다. 사과뿌리썩이선충은 딸기 자묘를 통해 식물기생선충이 감염되지 않은 건전한 토양으로의 확산이 확인되었다(33%).

• 한국식물병리학회지
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• 제1권1호
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• pp.28-32
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• 1985

딸기$\cdot$시들음병에 대한 실용적인 약제방제법을 규명하기 위하여 본 시험을 실시하였다. 이 균이 균사생장과 후막포자 발아의 저지효과에 있어서 Captafol이 가장 효과적이었으며 Benomyl도 저지효과가 현저하였다. 포장시험에서는 Captafol, Benomyl, Thiophanate-methly의 토양근주의 효과가 비교적 우수하였다. Captafol.의 토양근주에 의하여 본 병원균의 토양내 균량은 크게 감소되었으며, Trichoderma sp. 의 균량은 오히려 증가되었다.

• Mycobiology
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• 제37권2호
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• pp.77-81
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• 2009

This study was conducted to identify the Colletotrichum species causing anthracnose disease of strawberry in Balgladesh and to evaluate in vitro activity of commercial fungicides it. Based on morphological and cultural characteristics, all 22 isolates were identified as Colletotrichum gloeosporioides. They developed white or glittery colonies with grey to dark grey reverse colony colors and they produced cylindrical conidia. The efficacy of five commercial fungicides, Bavistin DF, Dithane M-45, Sulcox 50 WP, Corzim 50 WP and Rovral 50 WP, were tested against the fungus. Bavistin inhibited radial growth completely and was followed in efficacy by Dithane M-45. In Bavistin DF treated media, the fungus did not produce conidia. The percent inhibition of radial growth of the fungus was increased with the increasing concentrations of fungicide.