• Title/Summary/Keyword: Strain-control

Search Result 2,265, Processing Time 0.034 seconds

Accelerated Growth of Corynebacterium glutamicum by Up-Regulating Stress-Responsive Genes Based on Transcriptome Analysis of a Fast-Doubling Evolved Strain

  • Park, Jihoon;Lee, SuRin;Lee, Min Ju;Park, Kyunghoon;Lee, Seungki;Kim, Jihyun F.;Kim, Pil
    • Journal of Microbiology and Biotechnology
    • /
    • v.30 no.9
    • /
    • pp.1420-1429
    • /
    • 2020
  • Corynebacterium glutamicum, an important industrial strain, has a relatively slower reproduction rate. To acquire a growth-boosted C. glutamicum, a descendant strain was isolated from a continuous culture after 600 generations. The isolated descendant C. glutamicum, JH41 strain, was able to double 58% faster (td=1.15 h) than the parental type strain (PT, td=1.82 h). To understand the factors boosting reproduction, the transcriptomes of JH41 and PT strains were compared. The mRNAs involved in respiration and TCA cycle were upregulated. The intracellular ATP of the JH41 strain was 50% greater than the PT strain. The upregulation of NCgl1610 operon (a putative dyp-type heme peroxidase, a putative copper chaperone, and a putative copper importer) that presumed to role in the assembly and redox control of cytochrome c oxidase was found in the JH41 transcriptome. Plasmid-driven expression of the operon enabled the PT strain to double 19% faster (td=1.82 h) than its control (td=2.17 h) with 14% greater activity of cytochrome c oxidase and 27% greater intracellular ATP under the oxidative stress conditions. Upregulations of genes those might enhance translation fitness were also found in the JH41 transcriptome. Plasmid-driven expressions of NCgl0171 (encoding a cold-shock protein) and NCgl2435 (encoding a putative peptidyl-tRNA hydrolase) enabled the PT to double 22% and 32% faster than its control, respectively (empty vector: td=1.93 h, CspA: td=1.58 h, and Pth: td=1.44 h). Based on the results, the factors boosting growth rate in C. gluctamicum were further discussed in the viewpoints of cellular energy state, oxidative stress management, and translation.

Fabrication of Carbon Nanotube Strain Sensors (카본나노튜브 스트레인 센서 제작 기술)

  • Chang, Won-Seok;Song, Sun-Ah;Kim, Jae-Hyun;Han, Chang-Soo
    • Transactions of the Korean Society of Mechanical Engineers B
    • /
    • v.33 no.10
    • /
    • pp.773-777
    • /
    • 2009
  • In this study, the strain sensing characteristics of single-wall carbon nanotubes(SWCNTs) networks were investigated to develop a film sensor for strain sensing. The SWCNTs film are formed on flexible substrates of poly(ethylene terephthalate) (PET) using spray process. In this manner we could control the transparency and obtain excellent uniformity of the networked SWCNT film. The carbon nanotube film is isotropic due to randomly oriented bundles of SWCNTs. Using experimental results it is shown that there is a nearly linear change in resistance across the film when it is subjected to tensile stress. The results presented in this study indicate the potential of such films for high sensitive transparent strain sensors on macro scale.

Selection of a Highly Virulent Verticillium lecanii Strain Against Trialeurodes vaporariorum at Various Temperatures

  • Lee, Min-Ho;Yoon, Cheol-Sik;Yun, Tae-Yu;Kim, Hong-Sun;Yoo, Jai-Ki
    • Journal of Microbiology and Biotechnology
    • /
    • v.12 no.1
    • /
    • pp.145-148
    • /
    • 2002
  • The virulence of five Verticillium lecanii strains against greenhouse whiteflies, Trialeurodes vaporariorum, was tested at various temperatures as a major environmental factor. Strain CS-626 was found to be the most durable strain at a broad temperature range, and highly virulent against greenhouse whiteflies in a detached-leaf bioassay. In a tomato plant pot experiment, the $LT_{50}\;and\;LC_{50}$ of the CS-626 strain were 6.2 days and $2.3{\times}10^6$ conidia/ml, respectively. The optimal concentration of CS-626 for successful infection was $1{\times}10^8$conidia/ml. These results indicate that the CS-626 strain of Verticillium lecanii has a strong potential for effectively controlling greenhouse whiteflies.

Isolation and Characterization of Bacillus thuringiensis strain AF6 Producing an Antifungal Substance and a Mosquitocidal Delta-endotoxin Simultaneously (모기유충에 대한 살충성 내독소와 항진균성 물질을 동시에 생산하는 B. thuringiensis AF6균주의 분리 및 특성)

  • 김광현;이광배;신두만
    • Journal of environmental and Sanitary engineering
    • /
    • v.13 no.2
    • /
    • pp.40-46
    • /
    • 1998
  • For a biological control on a plant pathogen, Pryicularia Oryzae, and a mosquito, Aedes aegypti, Bacillus thuringiensis strain AF6 which produces parasporal inclusion, delta-endotoxin, was isolated. The B. thuringiensis strain AF6 was produced not only an antifungal substance(AFS) against P. oryzae, but also a mosquitocidal delta-endotoxin. The AFS of the strain AF6 in more stable at pH 4.0 than pH 10.0. At the mode of action, the AFS of the strain AF6 was inhibited hypha growth on potato agar plate(pH 5.0), and degraded cell walls of P. oryzae.

  • PDF

Subjective Evaluation for Recovery from Visual Strain in Video Data Terminal Operation - How to Recover from Visual Strain in VDT Operation -

  • Muraoka, Tetsuya;Nakashima, Noboru;Ikeda, Hiroaki;Ishizaki, Yoshiaki
    • 제어로봇시스템학회:학술대회논문집
    • /
    • 1999.10a
    • /
    • pp.189-193
    • /
    • 1999
  • For a video data terminal (VDT) operator, visual strain was caused by continuous VDT operations was found id be recovered by watching the picture of virtual far point with the background of the complementary color when the treatment to recover from visual strain was carried out. When the VDT operator watches the picture of virtual far point with the condition of the complementary color stimuli on the CRT display in 60 or 120 minutes after the start of the VDT operation, the visual strain is recovered and the VDT operator is kept healthy.

  • PDF

이중하중을 받는 S45C의 피로거동에 관한 연구

  • 윤두연;이원석;이현우
    • Proceedings of the Korean Society of Precision Engineering Conference
    • /
    • 1992.04a
    • /
    • pp.268-273
    • /
    • 1992
  • Thin walled tubular specimens of 0.45% structural carbon steel were used in the bizxial tests. Biaxial fatigue tosts were conducted on strain control including fully reversed tension-compression and in phase tension torsion loadings. The predictions of the biaxial fatigue life were based upon the uniaxial low cycle fatigue test results. Fatigue lives were ranged from 10$\^$2/to 10$\^$5/cycles. Four multiaxial strain based theories have been developed to correlate biaxial fatigue experimdntal results. These theories showed good correlatins except for maximum shear strain theory. In uniaxial tests, crack behavior was observed that crack initiated in the maximum shear strain direction and propagated in the direction perpendicular to principal stross. But, in biaxial tests, both crack initiation and growth occured on the maximum shear strain direction only.

Properties of Thermal Expansion Strain of Light Weight Aggregate Concrete with Loading Conditions (하중조건에 따른 경량골재 콘크리트의 열팽창변형 특성)

  • Yoon, Min-Ho;Kim, Gyu-Yong;Lee, Tae-Gyu;Nam, Jeong-Soo;Shin, Kyoung-Su;Lee, Sang-Soo
    • Proceedings of the Korean Institute of Building Construction Conference
    • /
    • 2012.05a
    • /
    • pp.255-256
    • /
    • 2012
  • In this study, strain properties of high strength concrete using light weight aggregate which is widely used in recent years are evaluated. For these purpose, thermal strain, transient creep were measured in prestressed condition as 0, 20, 40% of specimen strength at target temperature with 60MPa specimens which was using normal and light weight aggregate. As a result, light weight aggregate is more advantageous for the control of strain than normal aggregate because of its low thermal expansion.

  • PDF

Regulation of Tubercidin Biosynthesis in Streptomyces tubercidicus by Adenine and Histidine (Streptomyces tubercidicus에서 Adenine과 Histidine에 의한 Tubercidin 생합성 조절)

  • 유진철;하영칠
    • Korean Journal of Microbiology
    • /
    • v.29 no.3
    • /
    • pp.160-166
    • /
    • 1991
  • The regulatory mechanism of tubercidin biosynthesis in Streptomyces tubercidicus was studied. In a wild type strain, addition of adenine and histidine into the medium decreased the tubercidin production by 60-65% and 40%, respectively. The effects of adenine and histidine were alleviated by the addition of inosine monophosphate and 5-aminoimidazole-4-carboxamide ribotide. The production of tubercidin in S. tubercidicus K115 strain ($ade^{-}$ ) was nearly shut off by histidine. In contrast with K115 strain, adenine inhibited the tubercidin biosynthesis in S. tubercidicus K412 strain ($his^{-}$. In S. tubercidicus F667 strain ($ade^{-}$ , $his^{-}$ ), tubercidin production was increased by adenine and histidine. From the effects of adenine and histidine on tubercidin biosynthesis in S. tubercidicus wild type and mutant strains, it became known that feedback control by adenine and histidine of biosynthetic pathwat for purine ribonucleotide and histidine are involved in the regulation of tubercidin biosynthesis.

  • PDF

Pathogenicity of Entomopathogenic Nematodes to Some Agro-Forest Insect Pests (농림해충에 대한 곤충병원성 선충의 병원성)

  • 이상명;이동운;추호렬;김도완;김준범
    • The Korean Journal of Soil Zoology
    • /
    • v.2 no.2
    • /
    • pp.76-82
    • /
    • 1997
  • Mortality of some agro-forest insect pests, Agrotis segetum, Blitopertha orientalis, Agerastica coerulea, Glyphodes perspectalis and Acantholyda parki caused by entomopathogenic nematodes was investigated in laboratory and in pot. Steinernema carpocapsae All and Pocheon strain were effective against 2nd of 3rd instar larvae of A. segetum showing 100% mortalities at the concentration of 10Ijs/larva but not effective against 4th inster larvae. Mortality of B. orientalis was 100% after 26 days in H. bacteriopora NC 1, H. bacteriophora Hamyang and S. glageri NC strain treatment. S. monticulum also showed 100% mortalities against A. coerulea andd G. perspectalis at the concentration of 80 and 40 Ijs/larva. However, H. bacteriophora Cheju and S. glaseri Cheju strain were not effective against A. parki, i.e., showing 23.3 and 20.0% mortalities, respectively at the concentration of 160Ijs/larva. S. glaseri Hanrim strain was more effective than H. bacteriophora Cheju strain against Pryeria sinica.

  • PDF

The Test of the Isolation Hypothesis and the Buffer Hypothesis of Demand-Control-Support Model on the Elderly Women's Productive Activity (여성 고령자의 생산적 활동에 대한 요구-조절-지지 모델의 고립 긴장과 완충 효과 검증)

  • Cho, Yoon-Joo
    • Journal of Families and Better Life
    • /
    • v.26 no.5
    • /
    • pp.91-107
    • /
    • 2008
  • This study investigated the isolation hypothesis and the buffer hypothesis of Demand-Control-Support model in relation to activity satisfaction and psychological well-being. The subjects were 300 elderly women participating in productive activity for example paid work, voluntary activity, and grancdhildren care. This research tested four hypotheses concerning the DCS model. Is there support for the isolation hypothesis, such that the lowest level of activity satisfaction is experienced by the elderly women working in an isolation situation(high demand-low control-low support)? Is there support for the isolation hypothesis, such that the lowest level of psychological well-being is experienced by the elderly women working in an isolation situation(high demand-low control-low support)? Is there support for the buffer hypothesis, i. e. interaction between demand, control, and support, indicating a buffering effect of support on the negative impact of high strain on activity satisfaction? Is there support for the buffer hypothesis, i. e. interaction between demand, control, and support, indicating a buffering effect of support on the negative impact of high strain on psychological well-being? Major results of this study were as follows. and were supported. Activity satisfaction and psychological well-being of the elderly women in isolation situation was the lowest among the sample. was supported that family support level buffered the negative impact of high strain on activity satisfaction. But was not supported. Only main effect of demand level was showed on psychological well-being.