• Korean Journal of Veterinary Research
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• v.38 no.4
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• pp.793-802
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• 1998

Salmonellosis caused by a number of serotypes of Salmonella is an infectious, acute or chronic, zoonotic disease and characterized by enteritis and diarrhea, septicemia in animal. In these studies we investigated the prevalent serotypes of Salmonella causing animal salmonellosis in Korea and the 71 strains of Salmonella spp. were isolated from materials such as mesenteric lymph nodes, fecal samples from slaughtered animal. With the identification test results, the most prevalent serotypes were, in order, S stanley 31 strains (43.7%), S typhimurium 19 strains (26.8%) and S montevideo 11 strains (15.5%), respectively. And we could establish the method for detection of antibodies to broad variety of Salmonella serotypes. Lipopolysaccharide(LPS) antigen extracted from Salmonella was more sensitive and specific than outer membrane protein antigen from that for detection of Salmonella antibody by using an indirect ELISA. The optimal concentration of antigen was 100ng/ml of LPS, the dilutions of conjugate and serum were 1 : 1,000~2,000 and 1 : 200~400, respectively. The mix LPS-ELISA which was used by mixing LPS from S typhimurium (group B), S choleraesuis (group C) and S enteritidis (group D) were more rapid and effective than that used LPS from individual strain for detection of Salmonella serogroup O4, O7 and O9 antibody at the same time. We could obtain the high values of optical density ($0.73{\pm}0.32$) by mix LPS-ELISA on the farm which had occurred salmonellosis, but very low values of $0.17{\pm}0.06$ on the negative farm of salmonellosis. So, the mix LPS-ELISA may be used to monitor the serological surveillance for the presence of infection with a number of serotypes of Salmonella and would be useful for prevention and control of salmonellosis in animal.

• Journal of the Korea Concrete Institute
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• v.26 no.3
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• pp.287-297
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• 2014

Heat of hydration of mass concrete is one of the most important factors that significantly affect structural quality and construction period. Therefore, appropriate methods to control heat of hydration are essential technologies for mass concrete construction. In this study, probability of thermal cracking was checked by thermal analysis prior to the construction of a turbine foundation in a domestic power plant. Subsequently, changes of concrete mix proportion and an effective curing method were proposed to control heat of hydration of mass concrete structures. Concrete manufactured by slag cement was proposed instead of concrete produced by ordinary Portland cement, and an automated curing method was proposed to improve the curing method using typical moist curing with blanket. The automated curing method maintains the temperature difference between center and surface of concrete below a setting value by temperature monitoring. Concrete with slag cement was used for actual construction. One of two identical turbine foundations was cured by an insulated curing method, and the other was cured by the automated curing method to compare the curing methods. And then, the effects of control of heat of hydration were evaluated based on temperature/strain monitoring and crack investigations.

• The Journal of Engineering Geology
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• v.23 no.3
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• pp.283-292
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• 2013

A calcareous rock slope failed during excavation of the slope for construction of a tunnel entrance. The slope is located at the construction site for widening highway in Yeongwol, Korea. Field surveys, laboratory tests, and numerical analyses were performed to determine the reason for the slope failure. The numerical analysis revealed that the safety factor of the slope before construction of the entrance was less than 1, and that this decreased after construction. After construction of the entrance, the sliding zone of the slope increased and slope stability decreased because the shear strain and plastic zone in the slope over the tunnel entrance showed an increase relative to the lower part of the slope. To enhance the stability of the slope for construction of the tunnel entrance, countermeasures such as rock bolts, rock anchors, and FRP (Fiber glass Reinforced Plastic) grouting were adopted in light of the field conditions. Serial field monitoring performed to confirm the reinforcing effects of the adopted countermeasures revealed a small amount of horizontal deformation of the slope soils, most of the elastic deformation that can regain its former value. In addition, the axial forces of the rock bolt and anchor were more strongly affected by slope excavation during construction of the tunnel entrance than by tunnel excavation or the rainy season, and the axial forces tended to converge after excavation of the tunnel. Therefore, we can confirm that the slope is currently safe.

• Journal of the Korean Geosynthetics Society
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• v.16 no.4
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• pp.83-92
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• 2017

This paper deals with numerical analysis of behavior of curved mechanically stabilized earth(MSE) walls with geosynthetics reinforcement. Unlike typical concrete retaining walls, MSE wall enables securing stability of higher walls without being constrained by backfill height and is currently and widely used to create spaces for industrial and residential complexes. The design of MSE walls is carried out by checking external stability, similarly to the external checks of conventional retaining wall. In addition, internal stability check is mandatory. Typical stability check based on numerical analysis is done assuming 2-dimensional condition (plane strain condition). However, according to the former studies of 3-dimensional MSE wall, the most weakest part of a curved geosynthetic MSE wall is reported as the convex location, which is also identified from the studies of the laboratory model tests and field monitoring. In order to understand the behaviour of the convex location of the MSE wall, 2-dimensional analysis clearly reveals its limitation. Furthermore, laboratory model tests and field monitoring also have restriction in recognizing their behaviour and failure mechanism. In this study, 3-dimensional numerical analysis was performed to figure out the behaviour of the curved part of the geosynthetic reinforced wall, and the results of the straight-line and curved part in the numerical analysis were compared and analysed. In addition, the behaviour characteristics at each condition were compared by considering the overburden load and relative density of backfill.

• Journal of Microbiology and Biotechnology
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• v.19 no.4
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• pp.339-345
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• 2009

We evaluated the activity and abundance of the crude-oil-degrading bacterium Nocardia sp. H17-1 during bioremediation of oil-contaminated soil, using real-time PCR. The total petroleum hydrocarbon(TPH) degradation rate constants(k) of the soils treated with and without H17-1 were $0.103\;d^{-1}$ and $0.028\;d^{-1}$ respectively. The degradation rate constant was 3.6 times higher in the soil with H17-1 than in the soil without H17-1. In order to detect and quantify the Nocardia sp. H17-1 in soil samples, we quantified the genes encoding 16S ribosomal RNA(16S rRNA), alkane monooxygenase(alkB4), and catechol 2,3-dioxygenase(23CAT) with real-time PCR using SYBR green. The amounts of H17-1 16S rRNA and alkB4 detected increased rapidly up to 1,000-folds for the first 10 days, and then continued to increase only slightly or leveled off. However, the abundance of the 23CAT gene detected in H17-1-treated soil, where H17-1 had neither the 23CAT gene for the degradation of aromatic hydrocarbons nor the catechol 2,3-dioxygenase activity, did not differ significantly from that of the untreated soil($\alpha$=0.05,p>0.22). These results indicated that H17-1 is a potential candidate for the bioaugmentation of alkane-contaminated soil. Overall, we evaluated the abundance and metabolic activity of the bioremediation strain H17-1 using real-time PCR, independent of cultivation.

• Korean Journal of Veterinary Research
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• v.51 no.4
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• pp.267-275
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• 2011

Methicillin-resistant Staphylococcus (S.) aureus (MRSA) is one of the most important nosocomial pathogens worldwide and the emergence of this strain has become a major clinical problem. In this study, we investigated the prevalence of MRSA and their genetic characteristics in 69 S. aureus isolated from humans and animals. In human isolates, higher antimicrobial resistance rates were observed against penicillin (80.6%), followed by erythromycin (11.9%) and tetracycline (9.0%). All of them were susceptible to clindamycin, enrofloxacin, novobiocin, pirlimycin, trimethoprim/sulfamethoxazole and vancomycin. The resistance patterns in animal isolates were similar to those of human isolates. Two (2.9%) MRSA strains were isolated from human (n = 1) and animal (n = 1), and these isolates were confirmed as carrying the mecA gene. One isolate originating from human was resistant to 7 drugs and the other isolate derived from animal was resistant to 11 drugs. Staphylococcal cassette chromosome mec (SCCmec) variant IIIB was identified in animal isolate but SCCmec type of an isolate from human was not exactly determined. Two MRSA isolates showed unrelated PFGE pattern between them. Our results indicated although the frequency of MRSA isolates from humans and animals was low, a continuous surveillance and monitoring should be called for to prevent the contamination and spread of MRSA in the community. To our knowledge, this is the first time that SCCmec type variant IIIB was detected from animals in Korea.

• Geomechanics and Engineering
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• v.8 no.4
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• pp.541-557
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• 2015

This research aims to analyze the fracture coalescence characteristics of brittle sandstone specimen ($80{\times}160{\times}30mm$ in size) containing various flaws (a single fissure, double squares and combined flaws). Using a rock mechanics servo-controlled testing system, the strength and deformation behaviours of sandstone specimen containing various flaws are experimentally investigated. The results show that the crack initiation stress, uniaxial compressive strength and peak axial strain of specimen containing a single fissure are all higher than those containing double squares, while which are higher than those containing combined flaws. For sandstone specimen containing combined flaws, the uniaxial compressive strength of sandstone increase as fissure angle (${\alpha}$) increases from $30^{\circ}$ to $90^{\circ}$, which indicates that the specimens with steeper fissure angles can support higher axial capacity for ${\alpha}$ greater than $30^{\circ}$. In the entire deformation process of flawed sandstone specimen, crack evolution process is discussed detailed using photographic monitoring technique. For the specimen containing a single fissure, tensile wing cracks are first initiated at the upper and under tips of fissure, and anti-tensile cracks and far-field cracks are also observed in the deformation process; moreover anti-tensile cracks usually accompanies with tensile wing cracks. For the specimen containing double squares, tensile cracks are usually initiated from the top and bottom edge of two squares along the direction of axial stress, and in the process of final unstable failure, more vertical splitting failures are observed in the ligament region. When a single fissure and double squares are formed together into combined flaws, the crack coalescence between the fissure tips and double squares plays a significant role for ultimate failure of the specimen containing combined flaws.

• Journal of the Korean Society of Tobacco Science
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• v.37 no.1
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• pp.18-24
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• 2015

Urinary mutagenicity is widely recognized as a useful biomarker for the assessment of mutagen exposure level in human. In this study, we optimized the several parameters affecting the activity of Urinary mutagenicity using highly sensitive mutation test(microsuspension assay) instead of the conventional Ames test. First of all, we chose YG1024 as a highly sensitive strain from three str ains of Salmonella typhimurium(TA98, TA100, YG1024) using r epr esentative mutation substances, such as Benzo[a]pyrene, 2-Aminonaphthalene, 2-amino-3-methyl-9H-pyrido[2,3-b]indole($MeA{\alpha}C$) and cigarette total particulate matter(TPM). And we established the several kinds of test conditions such as number of bacter ia, concentr ation of metabolic activation system and incubation time for the most sensitive reaction. Also, we optimized efficient pre-treatment method using commercial C18 column. As a r esults, this method was shown a aver age of 94 % recovery value and 13 % relative standard deviation. When we compared the Urinary mutagenicity between several participants, we confirmed that compar ative measurements were possible for different levels of urine mutagenicity. In conclusion, the optimized highly sensitive mutation test to measure the Urinary mutagenicity may be useful in biological monitoring of mutagen exposure level.

• Journal of the Ergonomics Society of Korea
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• v.23 no.3
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• pp.121-134
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• 2004

A research project was conducted to study work-related musculoskeletal disorders （MSDs） at subway train repair plant in Korea. The project was consisted of 4 main parts; education on the topics of MSDs for all workers, symptom survey, medical check-up, investigation of MSD risk factors. The result of symptom survey showed that 95.2％ of the respondents complained pains on at least one part of the body. After cross-sectional analysis of various information and risk factors, 86.8％ of respondents were considered as active health surveillance level 1 that require continuous monitoring on their symptoms and working conditions. And 57.1％ of the respondents were considered as active health surveillance level 2 that needed medical check-ups for proper medical treatment. The analysis of occupational risk factors revealed that handling of heavy object (46.15％) and repeated awkward postures (46.15％) were two most contributing risk factors for the on-set of MSD at this work site followed by static strain (7.7％), and vibration and impact (3.8％). Medical examination was performed by an industrial medicine MD on 156 workers those considered as active health surveillance level 2. The result showed that 35 workers (20.7％) were considered as MSD patient group at severe level, and 68.6 workers (68.6％) were considered as moderate group and 18 workers (10.6％) were considered minor or normal group those have no symptom.

• Journal of the Korean Society for Nondestructive Testing
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• v.27 no.2
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• pp.115-123
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• 2007

For investigating self-diagnosis applicability, a method based on monitoring the changes in the electrical resistance of carbon fiber reinforced concrete has been tested. Then after examining change in the value of electrical resistance at each flexural weight-stage of carbon fiber in CFGFRP (carbon fiber and glass fiber reinforcing plastic) with new type rib and carbon sheet for concrete reinforcing, the correlations of electrical resistance and load as a function of strain, deflection were analyzed. As the results, it is clarified that when carbon fiber rod, rib and sheet fracture, the electrical resistance of it increase largely, and specially in case of CFGFRP, afterwards glass fiber tows can be resist the load due to the presence of the hybrid (carbon and glass) reinforced fiber. Therefore, it can be recognized that reinforcing bar and new type rib of CFGFRP and sheet of CF could be applied for self-diagnosis of fracture in reinforced FRP concrete.