• Mycobiology
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• v.42 no.1
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• pp.46-51
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• 2014

A degenerated strain of Pleurotus eryngii KNR2312 was isolated from a commercial farm. Random amplified polymorphic DNA analysis performed on the genomic DNA of the normal and degenerated strains of this species revealed differences in the DNA banding pattern. A unique DNA fragment (1.7 kbp), which appeared only in the degenerated strain, was isolated and sequenced. Comparing this sequence with the KNR2312 genomic sequence showed that the sequence of the degenerated strain comprised three DNA regions that originated from nine distinct scaffolds of the genomic sequence, suggesting that chromosome-level changes had occurred in the degenerated strain. Using the unique sequence, three sets of PCR primers were designed that targeted the full length, the 5' half, and the 3' half of the DNA. The primer sets P2-1 and P2-2 yielded 1.76 and 0.97 kbp PCR products, respectively, only in the case of the degenerated strain, whereas P2-3 generated a 0.8 kbp product in both the normal and the degenerated strains because its target region was intact in the normal strain as well. In the case of the P2-1 and P2-2 sets, the priming regions of the forward and reverse primers were located at distinct genomic scaffolds in the normal strain. These two primer sets specifically detected the degenerate strain of KNR2312 isolated from various mushrooms including 10 different strains of P. eryngii, four strains of P. ostreatus, and 11 other wild mushrooms.

• The Journal of Korean Academy of Prosthodontics
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• v.40 no.3
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• pp.236-245
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• 2002

The acrylic resin was first introduced as denture base materials in 1937 and it is commonly used for denture base fabrication nowadays. Three different curing cycles (Conventional curing cycle, short curing cycle and long curing cycle) and three commercially available heat-activated acrylic resins (Vertex RS, Lucitone 199 and ProBase Hot) were investigated to find the curing cycle and material that showed the minimum shrinkage of the resin during polymerization process. A brass master mold was fabricated and duplicated by additional silicone impression material. Stone molds were made by pouring of type III dental stone (SILKY-ROCK YELLOW, Whip-Mix, Louisville, Kentucky). It was embedded in the flask. Strain gauge and thermocouple were embedded in the specimen. Strain gauge and thermocouple were connected to signal conditioning amplifier and data was recorded by pre-programmed software. The parameters ESmax (Maximum expansion strain), Sb (Strain measured just before deflasking procedure), Sa (Strain measured just after deflasking procedure) and Sf (Strain measured at the end of the experiment) were measured. ${\Delta}$S was calculated from Sb and Sa (${\Delta}$S=Sb-Sa). In the experiment concerned about materials, the parameters 90-ESmax (Maximum expansion strain measured during early 90 minutes of curing procedure), 180-ESmax (Maximum expansion strain measured from 90 minutes to 180 minutes), Sb, Sa, ${\Delta}$S and Sf were measured and the following conclusions were made. 1. The ESmax value of conventional curing cycle showed the largest value and the 180-ESmax value of Lucitone 199 showed the smallest value. 90-ESmax values showed no significant difference (p<0.05). 2. ${\Delta}$S values of conventional curing cycle showed the positive values. ${\Delta}$S values of short curing cycle and long curing cycle showed the negative values. All three materials cured by conventional curing cycle showed the positive values. 3. The Sf values of long curing cycle and ProBase Hot (cured by conventional curing cycle) showed the smallest values.

• Journal of the Korea Concrete Institute
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• v.13 no.3
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• pp.268-276
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• 2001

A numerical model for the thermal response analysis of concrete structures is suggested. The model includes the stress-strain relationship, constitutive relationship, and multiaxial failure criteria at elevated temperature conditions. Modified Saenz's model was used to describe the stress-strain relationship at high temperatures. Concrete subjected to elevated temperatures undergoes rapid strain increase and dimensional instability. In order to explain those changes in mechanical properties, a constitutive model of concrete subjected to elevated temperature is proposed. The model consists of four strain components; free thermal creep strain, stress-induced (mechanical) strain, thermal creep strain, and transient strain due to moisture effects. The failure model employs modified Drucker-Prager model in order to describe the temperature dependent multiaxial failure criteria. Some numerical analyses are performed and compared with the experimental results to verify the proposed model. According to the comparison, the suggested material model gives reliable analytical results.

• Transactions of the Korean Society of Mechanical Engineers
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• v.12 no.5
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• pp.935-943
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• 1988

The forming limit diagram is assessed as a means of estimating the forming characteristics of sheet metal and is usually determined experimentally. The strain rates used in the determination are likely to be low. However, often in practice, the strain rates are much higher, so if forming limit diagram is determined at low rates, it may not be appropriate. This paper reconsiders the forming limit diagram for mild steel and aluminum sheet up to variation in strain rate from 10$^{-2}$ sec to 20/sec where its forming has been carried out under oil pressure using a hydraulic bulge test with circular and elliptical dies. To obtain higher strain rate, an impact bulge test had been employed with the same die sets as those used for a hydraulic bulge test. The results obtained are as follows: (1) As the strain rate increases, the fracture pressure increases and the polar height at fracture decreases. (2) Experiment has shown that, in the positive quadrant of the forming limit diagram, the diagram is lowered with increasing strain rate and the effect of strain rate changes according to strain paths and materials..

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2003.05a
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• pp.38-43
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• 2003

The dimensions of die and workpiece are changed continuously during loading, unloading, and ejecting stage. Finally, to predict precisely the dimension of forged part and get the die dimension for the net-shape components, the analysis of die and workpiece should be evaluated from the loading to ejecting. Therefore, the experimental and FEM analysis are peformed to investigate the elastic characteristics at workpiece and die in the closed-die upsetting for ferrous material. FE techniques are proposed to consider the unloading and ejecting stages and estimate more precisely the dimension of forged part and die. The dimensional changes for the workpiece were evaluated quantatively during loading, unloading, and ejecting stages. The strains measured by the strain gages were compared with the estimated values by the FEM.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 1994.03a
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• pp.71-79
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• 1994

Strip drawing of strain-hardening, viscoplastic materials with damage is analyzed by a rigid plastic finite element method. A process model is formulated using two state variables, one for strain hardening from slip dominated plastic distortion and the other for damage from growth of microvoids. Application of the model to steady state drawing is given via implementation in a consistent penalty finite element formulation. The predicted density changes as a result of void growth are compared to those from experiments reported in the literature. The effects of drawing conditions such as drawing speed and die angle on the mechanical property changes are studied.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2005.11b
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• pp.77-80
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• 2005

This paper presents a theoretical and experimental study on the damage identification of structures. In civil and aerospace, significant work has been done in the area of detecting damage in structures by using changes in the dynamic response of the structure. In this paper a method based on the changes in the strain energy of the structure will be discussed. To evaluate the effectiveness of the method it will be applied to both beam and LNG(liquefied natural gas) carrier.

• Journal of Microbiology and Biotechnology
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• v.19 no.6
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• pp.547-555
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• 2009

Nanoscopic changes in the cell surface morphology of the yeasts Saccharomyces cerevisiae (strain NCYC 1681) and Schizosaccharomyces pombe (strain DVPB 1354), due to their exposure to varying concentrations of hydrogen peroxide (oxidative stress), were investigated using an atomic force microscope (AFM). Increasing hydrogen peroxide concentration led to a decrease in cell viabilities and mean cell volumes, and an increase in the surface roughness of the yeasts. In addition, AFM studies revealed that oxidative stress caused cell compression in both S. cerevisiae and Schiz. pombe cells and an increase in the number of aged yeasts. These results confirmed the importance and usefulness of AFM in investigating the morphology of stressed microbial cells at the nanoscale. The results also provided novel information on the relative oxidative stress tolerance of S. cerevisiae and Schizo pombe.

• Transactions of Materials Processing
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• v.12 no.7
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• pp.662-667
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• 2003

The dimensions of die and workpiece are changed continuously during loading, unloading, and ejecting stage. Finally, to predict precisely the dimension of forged part and get the die dimension for the net-shape components, the analysis of die and workpiece should be evaluated from the loading to ejecting. Therefore, the experimental and FEM analyses are performed to investigate the elastic characteristics at workpiece and die in the closed-die upsetting for ferrous material FE techniques are proposed to consider the unloading and ejecting stages and estimate more precisely the dimension of forged part and die. The dimensional changes fur the workpiece were evaluated quantatively during loading, unloading, and ejecting stages. The strains measured by the strain gages were compared with the estimated values by the FEM.

• Korean Journal of Veterinary Research
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• v.64 no.1
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• pp.4.1-4.5
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• 2024

In this study, almost complete genomic sequences of porcine parvovirus (PPV)1 and PPV4 circulating in commercial pig farms in South Korea were obtained and analyzed. Important mutations that may be precursors to host changes, such as premature stop codons of PPV1 and frameshift mutations of PPV4, were observed in these sequences. A 27a-like strain of PPV1, known to show a lack of cross- neutralization against existing commercial vaccine strains, was identified by phylogenetic analysis. Given the active genetic evolution, the additional precursors to host changes and emerging new genotypes of PPVs need to be monitored through continuous sampling and genetic analysis.