• Journal of Korean Society on Water Environment
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• v.34 no.1
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• pp.46-56
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• 2018

A mcyB-specific Ultra-Rapid quantitative PCR was developed for the quantitative detection of Microcystis aeruginosa, which is often a dominant species in green tide. McyB-specific UR-qPCR was optimized under extremely short times of each step in thermal cycles, based on the specific primers deduced from the mcyB in microcystin synthetase of M. aeruginosa. The M. aeruginosa strain KG07 was used as a standard for quantification, after the microscopic counting and calculation by mcyB-specific UR-qPCR. The water samples from the river water with the Microcystis outbreak were also measured by using both methods. The $1.0{\times}10^8$ molecules of mcyB-specific DNA was recognized inner 4 minutes after beginning of UR-qPCR, while $1.0{\times}10^4$ molecules of mcyB-specific templates was detected inner 7 minutes with quantitative manner. From the range of $1.0{\times}10^2$ to $1.0{\times}10^8$ initial molecules, quantification was well established based on $C_T$ using mcyB-specific UR-qPCR (Regression coefficiency, $R^2=0.9977$). Between the numbers of M. aeruginosa cell counting under microscope and calculated numbers using mcyB-specific UR-qPCR, some differences were often found. The reasons for these differences were discussed; therefore, easy compensation method was proposed that was dependent on the numbers of the cell counting. Additionally, to easily extract the genomic DNA (gDNA) from the samples, a freeze-fracturing of water-sample using liquid nitrogen was tested, by excluding the conventional gDNA extraction method. It was also verified that there were no significant differences using the UR-qPCR with both gDNAs. In conclusion, the mcyB-specific UR-qPCR that we proposed would be expected to be a useful tool for rapid quantification and easy monitoring of M. aeruginosa in environmental water.

• Journal of the Korea Concrete Institute
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• v.23 no.4
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• pp.413-420
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• 2011

The test results of high-strength concrete beam specimens, which have various combinations of different types of flexural reinforcement and short fibers, were compared with the prediction results of codes, guidelines and models proposed by researchers. The theoretical calculation based on the ultimate strength method of the KCI and ACI Code underestimated the ultimate moments of FRP bar-reinforced beams without fibers. The models proposed by ACI 544.4R and Campione predicted the ultimate moment capacities inaccurately for the FRP bar-reinforced beam with steel fibers, because these models do not consider the increased ultimate compressive strain of fiber reinforced concrete. Bischoff's deflection model predicted the service load deflections reasonably well, while the deflection model of ACI Committee 440 underestimated the deflection of FRP bar-reinforced beams. Because the ACI 440 expression, used to predict member deflection, cannot directly apply to the beams reinforced with different types of reinforcing bars, an alternative method to estimate the deflections of beams with different types of reinforcing bars using the ACI 440 expression was proposed. In addition, Bischoff's approach for computing deflection was extended to include deflection after yielding of the steel reinforcement in the beams reinforced with steel and FRP bars simultaneously.

• Fire Science and Engineering
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• v.27 no.6
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• pp.50-56
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• 2013

The radiation effects on the auto-ignition and extinction characteristics of a non-premixed fuel-air counterflow field were numerically investigated. A detailed reaction mechanism of GRI-v3.0 was used for the calculation of chemical reactions and the optically-thin radiation model was adopted in the simulations. The flame-controlling continuation method was also used in the simulation to predict the auto-ignition point and extinction limits precisely. As a result, it was found that the maximum H radical concentration, $(Y_H)_{max}$, rather than the maximum temperature was suitable to understand the ignition and extinction behaviors. S-, C- and O-curves, which were well known from the previous theory, were identified by investigating the $(Y_H)_{max}$. The radiative heat loss fraction ($f_r$) and spatially-integrated heat release rate (IHRR) were introduced to grasp each extinction mechanism. It was also found that the $f_r$ was the highest at the radiative extinction limit. At the flame stretch extinction limit, the flame was extinguished due to the conductive heat loss which attributed to the high strain rate although the heat release rate was the highest. The radiation affected on the radiative extinction limit and auto-ignition point considerably, however the effect on the flame stretch extinction limit was negligible. A stable flame regime defined by the region between each extinction limit became wide with increasing the fuel temperature.

• Journal of Korean Tunnelling and Underground Space Association
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• v.16 no.6
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• pp.521-531
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• 2014

This paper is a study for behaviour of cavern type anchorage tunnels for suspension bridges with cable tension. Anchorage behaviour, design method for anchorage, and failure surface angle, ${\delta}$ are analyzed by comparing numerical analysis results and ultimate pullout capacities($P_u$) using bilinear corelation equation. Results show that design depths for cavern type anchorage tunnels are easily checked with linear relationships for $P/{\gamma}/H$ vs. displacement and $P_u/{\gamma}/H$ vs. H/b. The analysis results of maximum shear strain distribution and plastic status show that failure shapes are closer to circular arc model than soil cone model which frequently used. To an easy calculation of the ultimate pullout capacity, we propose a simple bilinear failure model in this study. The calculated ultimate pullout capacities from the proposed bilinear corelation equation using two failure angles results are similar to the ultimate pullout capacities from numerical analysis.

• Journal of the Korean Society for Nondestructive Testing
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• v.30 no.1
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• pp.6-12
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• 2010

Fiber optic sensor using fiber Bragg grating(FBG) probes is used for monitoring strain and temperature distributed on the wide surfaces of large structures. In this paper, in order to use many FBG probes in one optical fiber line, we propose a complex multiplexing technology which is composed of two techniques, one is time division multiplexing and another is wavelength division multiplexing. However, we only investigate the characteristics of time division multiplexing because FBG sensors basically can be operated by wavelength division multiplexing. We calculate the optimal reflectivities and the lengthwise location of five FBG probes in serial connection in order to obtain the unique reflected intensities from the FBG probes. We fabricate five FBG probes with the reflectivities of 13%, 16%, 25%, 40% and 80%, which are determined by the theoretical calculation, and observe the signal reflected from each FBG in the time domain from the experiment. There are differences between experimental and theoretical results caused by the signal noise and the differences of reflectivities of FBG probes. But the experimental results shows the reflected signals of five FBG probes which prove the availability of complex multiplexing.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.13 no.2
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• pp.245-256
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• 2000

A moment-curvature relationship to simulate the behavior of reinforced concrete beam under cyclic loading is introduced. Unlike previous moment-curvature models and the layered section approach, the proposed model takes into consideration the bond-slip effect by using monotonic moment-curvature relationship constructed on the basis of the bond-slip relation and corresponding equilibrium equation at each nodal point. In addition, the use of curved unloading and reloading branches inferred from the stress-strain relation of steel gives more exact numerical result. The advantages of the proposed model, comparing to layered section approach, may be on the reduction in calculation time and memory space in case of its application to large structures. The modification of the moment-curvature relation to reflect the fixed-end rotation and pinching effect is also introduced. Finally, correlation studies between analytical results and experimental studies are conducted to establish the validity of the proposed model.

• Journal of the Korean Geotechnical Society
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• v.37 no.9
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• pp.5-12
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• 2021

Pseudo-static slope stability analysis method is widely used in engineering practice to calculate the seismic factor of safety of slope subjected to earthquake ground motions. Although the dynamic analysis method is well recognized to have the primary advantage of simulating the stress-strain response of soils, it is not often used in practice because of the difficult in estimating the factor of safety. In this study, a procedure which utilizes the dynamic analysis method to extract the transient dynamic factor of safety is devleoped. This method overcomes the major limitation of the pseudo-static method, which uses an empirically determined seismic coefficient to derive the factor of safety. The proposed method is applied to a slope model and the result is compared with that of the pseudo-static method. It is shown that minimum dynamic factor of safety calculated by the dynamic analysis is slightly larger than that determined from the pseudo-static method. It is also demonstrated that the dynamic factor of safety becomes minimum when the horizontal seismic coefficient and horizontal average acceleration are maximum.

• Korean Journal of Ecology and Environment
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• v.47 no.4
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• pp.350-357
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• 2014

Harmful Algal Bloom Alert System (HABAS) for drinking water supply is require to fast and accurate count as system monitoring of cyanobacterium occurrence and inducing a response action. We measured correlation between colony size and cell number including genus Anabaena, Aphanizomenon, Microcystis, Oscillatoria which are targeted at HABAS, deducted from standard formula, and suggested calculation method from colony size to the number of cell. We collected cyanobacteria samples at Han River (Paldang reservoir), Nakdong River (Dalseong weir, Changnyeonghaman weir) and Geum River (Gobok reservoir) from August to October, 2013. Also, we studied correlation between colony size and cell number, and calculated regression equation. As a result of correlation of harmful cyanobacteria by genus, Anabaena spp. and Aphanizomenon spp. having trichome showed high correlation coefficients more than 0.93 and Microcystis spp. having colony showed correlation coefficient of 0.76. As a result of correlation of harmful cyanobacteria by species, Anabaena crassa, Aphanizomenon flos-aquae, A. issatschenkoi, Oscillatoria curviceps, O. mougeotii having trichome showed high correlation coefficients from 0.89 to 0.96, and Microcystis aeruginosa, M. wessenbergii, M. viridis having colony showed correlation coefficients from 0.76 to 0.88. Compared with other genus Microcystis relatively showed low correlation because even species and colony size are the same, cell density and cell size are different from Microcystis strains. In this study, using calculated regression might be fast and simple method of cell counting. From now on, we need to secure additional samples, and make a decision to study about other species.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.37 no.1
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• pp.67-76
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• 2024

In this paper, we present a DIP-MLS testing method that combines digital image processing with a rigid body-based MLS differencing approach to measure mechanical variables and analyze the impact of target location and image resolution. This method assesses the displacement of the target attached to the sample through digital image processing and allocates this displacement to the node displacement of the MLS differencing method, which solely employs nodes to calculate mechanical variables such as stress and strain of the studied object. We propose an effective method to measure the displacement of the target's center of gravity using digital image processing. The calculation of mechanical variables through the MLS differencing method, incorporating image-based target displacement, facilitates easy computation of mechanical variables at arbitrary positions without constraints from meshes or grids. This is achieved by acquiring the accurate displacement history of the test specimen and utilizing the displacement of tracking points with low rigidity. The developed testing method was validated by comparing the measurement results of the sensor with those of the DIP-MLS testing method in a three-point bending test of a rubber beam. Additionally, numerical analysis results simulated only by the MLS differencing method were compared, confirming that the developed method accurately reproduces the actual test and shows good agreement with numerical analysis results before significant deformation. Furthermore, we analyzed the effects of boundary points by applying 46 tracking points, including corner points, to the DIP-MLS testing method. This was compared with using only the internal points of the target, determining the optimal image resolution for this testing method. Through this, we demonstrated that the developed method efficiently addresses the limitations of direct experiments or existing mesh-based simulations. It also suggests that digitalization of the experimental-simulation process is achievable to a considerable extent.

• Tunnel and Underground Space
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• v.30 no.3
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• pp.256-269
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• 2020

The objective of this study is to evaluate the stress thresholds in crack development and the corrected fracture toughness of KURT granite under dry and saturated conditions. The stress thresholds were identified by calculation of inelastic volumetric strain from an uniaxial compression test. The corrected fracture toughness was estimated by using the Level II method (Chevron Bend specimen), suggested by ISRM (1988), in which non-linear behaviors of rock was taken into account. Average crack initiation stress(σ_ci) and crack damage stress(σ_cd) under a dry condition were 91.1 MPa and 128.7 MPa. While, average crack initiation stress(σ_ci) and crack damage stress(σ_cd) under a saturated condition were 58.2 MPa and 68.2 MPa. The crack initiation stress and crack damage stress of saturated ones decreased 36% and 47% respectively compared to those of dry specimens. A decrease in crack damage stress is relatively larger than that of crack initiation stress under a saturated condition. This indicates that the unstable crack growth can be more easily generated because of the saturation effect of water compared to the dry condition. The average corrected fracture toughness of KURT granite was 0.811 MPa·m^0.5. While, the fracture toughness of saturated KURT granite(K_CB) was 0.620 MPa·m^0.5. The corrected fracture toughness of rock in saturated condition decreases by 23.5% compared to that in dry condition. It is found that the resistance to crack propagation decreases under the saturated geological condition.