• Journal of Microbiology and Biotechnology
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• v.32 no.10
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• pp.1219-1225
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• 2022

Microorganisms involved in food fermentation not only improve the aroma and taste of the food, but also enhance its preservation. Thus, they are added as starter cultures to boost the final product quality of commercial fermented foods. Although these microorganisms originate from fermented foods and have a long history of consumption, the European Union recently applied the concept of Qualified presumption of Safety (QPS), which is a safety evaluation system for microorganisms used in food or feed in Europe. The QPS system is a species-level safety system and shares results with the European Novel Food System, a strain-level safety evaluation system. In the United States, microorganisms added to fermented foods are considered as food additives or Generally Recognized as Safe substance. In Korea, food microbe lists are presented at the species level. Moreover, the nation has established a strain-oriented evaluation system that applies temporary safety evaluation methods for food raw materials as well as new raw materials. However, when it comes to microorganisms isolated from traditional fermented foods and other fermented food products, there is no definition of the term "species," and there is a lack of an evaluation system at the species level. Therefore, such an evaluation system for microbial species used in Korean fermented foods is necessary.

• Korean Journal of Veterinary Service
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• v.46 no.3
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• pp.193-202
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• 2023

Porcine epidemic diarrhea is an infectious intestinal disease caused by the porcine epidemic diarrhea virus (PEDV). Especially, when suckling piglets are infected, the mortality rate is close to 100%. PEDV is classified into G1 and G2 types based on genetic differences. The G2 type PEDV outbreak in the United States in 2013 was highly pathogenic and contagious, and it has spread worldwide and caused continuous economic losses. Most commercial vaccines used are G1 type vaccines, and existing vaccines do not fully protect piglets due to genetic differences. In this study, we evaluated the safety of the newly developed G2 type attenuated HSGP vaccine strain by inoculating it into piglets and testing whether the vaccine virus spreads to the non-vaccinated, negative pigs and whether the vaccine reverts to its virulence during serial passage experiments. Each experiment lasted for 7 days for each passage, and fecal viral titers, clinical symptoms, and weight gain were measured daily. After the experiment, necropsy was performed to measure intestinal virus titer and pathological evaluation. As a result of the first passage, no transmission of the vaccine virus to negative pigs co-housed with vaccinated pigs was observed. In addition, after four consecutive passage experiments, the clinical symptoms and small intestine lesions were gradually alleviated, and no virus was detected in the feces in the fourth passage experiment. Therefore, it was concluded that the vaccine was safe without virulence reversion in accordance with the guidelines of the current licensing authority. However, further studies are needed on the genetic changes and biological characteristics of the mutant virus that occur during successive passages of the attenuated vaccine since the replication and clinical symptoms of the virus increased until the third passage during successive passages of the vaccine virus. Based on this study, it was concluded that virulence reversion and safety evaluation of attenuated vaccines through serial passage in target animals can be useful to evaluate the safety of attenuated viruses.

• Journal of the Korean Society for Heat Treatment
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• v.36 no.5
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• pp.311-317
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• 2023

An autofilter is a device that removes impurities contained in heavy fuel oil used in diesel engines of ships or power plants, and also automatically removes impurities accumulated in the filter through a reverse washing function. The reducer-integrated motor serves to rotate the filter at low speed to enable reverse automatic cleaning in the autofilter device. To achieve a low speed of 0.65 to 0.75 rpm in a reducer-integrated motor, a small motor that can operate at 97rpm at a rated voltage of 110 V and 112.5 rpm at 220 V is required. Additionally, a large gear ratio of 1/150 is required. To ensure the durability and reliability of these reducers, the strength of the gear must be evaluated at the design stage. In general, there is a limit to evaluating the stress and strain state according to the vibration characteristics acting on each gear in the driving state of the reducer through quasi-static analysis. Therefore, in this study, the operation characteristics of the auto filter's reducer-integrated motor were first analyzed using the rigid body dynamics analysis method. Then, this rigid body dynamics analysis model was extended to a flexible multibody dynamics analysis model to analyze the stress and strain states acting on each gear and evaluate the design feasibility of the gear.

• Journal of the Korea institute for structural maintenance and inspection
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• v.12 no.1
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• pp.158-166
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• 2008

Member force, strain and stress distribution of a section are obtained for prestressed steel and concrete(PSSC) composite bridge subjected to dead and live load in order to interpret the effect of prestressing and deformation of tendon. The stress and strain distribution and moment capacity are obtained for both noncomposite and composite section and for allowable stress limit state, yield limit state and strength limit state. Reliability analysis is conducted after assuming limit states for deflection, stress and flexural strength. Comparing that the reliability index for stress is near 0 for example section which is designed to satisfy the allowable stress exactly, the reliability indexes for deflection and flexural strength are high. Reliability of PSSC girder which is designed based on allowable stress of bridge design code is high for deflection and flexural strength.

• Journal of the Korea institute for structural maintenance and inspection
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• v.12 no.6
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• pp.214-224
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• 2008

Member force, strain and stress distribution of a section are obtained for optimized standard 25m~45m PSSC composite bridge subjected to dead and live load in order to interpret the effect of prestressing and deformation of tendon. The stress and strain distribution and moment capacity are obtained for both noncomposite and composite section and for allowable stress limit state, yield limit state and strength limit state. Reliability analysis is conducted after assuming limit states for stress and flexural strength. The reliability index for standard PSSC composite bridge which is designed to satisfy the allowable stress for flexural strength are higher than 3.5 which is required reliability indexes on American code for LRFD. Reliability of PSSC girder which is designed based on allowable stress of bridge design code is high for flexural strength.

• Steel and Composite Structures
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• v.52 no.4
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• pp.391-403
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• 2024

The flexural behavior of composite sandwich wall panels with different thicknesses, numbers of holes, and hole forms, and arrangement form of longitudinal steel bar (uniform type and concealed-beam type) are investigated. A total of twelve composite sandwich wall panels are prepared, utilizing modified polystyrene particles mixed with foam concrete for the flexural performance test. The failure pattern of the composite sandwich wall panels is influenced by the extruded polystyrene panel (XPS) panel thickness and the reinforcement ratio in combination, resulting in both flexural and shear failure modes. Increasing the XPS panel thickness causes the specimens to transition from flexural failure to shear failure. An increase in the reinforcement ratio leads to the transition from flexural failure to shear failure. The hole form on the XPS panel and the steel bar arrangement form affect the loading behavior of the specimens. Plum-arrangement hole form specimens exhibit lower steel bar strain and deflection compared to linear-arrangement hole form specimens. Additionally, specimens with concealed beam-type steel bar display lower steel bar strain and deflection than uniform-type steel bar specimens. However, the hole form and steel bar arrangement form have a limited impact on the ultimate load. Theoretical formulas for cracking load are provided for both fully composite and non-composite states. When compared to the experimental values, it is observed that the cracking load of the specimens with XPS panels closely matches the calculations for the non-composite state. An accurate prediction model for the ultimate load of fully composite wall panels is developed. These findings offer valuable insights into the behavior of composite sandwich wall panels and provide a basis for predicting their performance under various design factors and conditions.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2007.05a
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• pp.118-121
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• 2007

Bulk metallic glass (BMG)/crystalline composites comprising a copper based BMG alloy and crystalline nickel were produced by means of eloctroless plating of nickel on $Cu_{54}Zr_{22}Ti_{18}Ni_6$ BMG powder and subsequent consolidation using spark plasma sintering. The plastic deformation behavior of BMG/crystalline composites was examined by uniaxial compression test at various temperatures in the supercooled liquid region (SLR) of the BMG alloy. The evolution of strain states during uniaxial compression was tackled by microstructure observations. Deformation temperature played an important role in the deformation behavior of BMG/crystalline composites, which was attributed to a strong temperature dependence of the flow stress of the BMG alloy in the SLR. BMG/crystalline composites deformed homogenously in the temperature range where the flow stress of the BMG alloy was close to that of crystalline nickel. In contrast, inhomogeneous deformation was observed in the temperature range where the flow stress of the BMG alloy largely differs from that of crystalline nickel.

• Geotechnical Engineering
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• v.11 no.3
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• pp.43-54
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• 1995

The aim of the work described in this paper is to develope a constitutive model for the prediction of an unsaturated soil and to confirm the application'of the model, which is composed of the elastic and plastic part in consideration of the matric suction and the net mean stress. From test results, volume changes and deviator stresses are analyzed at each state and their relationships are formulated. The application of the model to silty sands is confirmed by the comparison between test and predicted results. During drying -wetting and loading -unloading processes for isotropic states, the agreement between predicted and test results are satisfactory. Predicted deviator stresses are well agreed with test results in shearing process. Overall acceptable predictions are reproduced in high confining pressure. Usefulness of the model is confirmed for the unsaturated soil except volumetric strain, which is not well agreed with the test results due to deficiency of dilatancy of the model in low confining pressure. It is, therefore. recommended to study the behavior of dilatancy for an unsaturated soil.

• Advances in materials Research
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• v.1 no.2
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• pp.115-128
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• 2012

The structural and electrical properties of $(1-x)Ba(Sm_{1/2}Nb_{1/2})O_3-xBaTiO_3$; ($0{\leq}x{\leq}1$) ceramics were prepared by conventional ceramic technique at $1375^{\circ}C$/7 h in air atmosphere. The crystal symmetry, space group and unit cell dimensions were derived from the X-ray diffraction (XRD) data using FullProf software whereas crystallite size and lattice strain were estimated from Williamson-Hall approach. XRD analysis of the compound indicated the formation of a single-phase cubic structure with the space group Pm m. Dielectric study revealed that the compound $0.75Ba(Sm_{1/2}Nb_{1/2})O_3-0.25BaTiO_3$ is having low and ${\varepsilon}^{\prime}$ and ${\varepsilon}^{{\prime}{\prime}}$ a low $T_{CC}$ (< 5%) in the working temperature range (up to+$100^{\circ}C$) which makes this composition suitable for capacitor application and may be designated as 'Stable Low-K' Class I material as per the specifications of the Electronic Industries Association. The correlated barrier hopping model was employed to successfully explain the mechanism of charge transport in the system. The ac conductivity data were used to evaluate the density of states at Fermi level, minimum hopping length and apparent activation energy of the compounds.

• Journal of Microbiology
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• v.42 no.1
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• pp.14-19
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• 2004

Salmonella enterica serovar Typhimurium DT104 (Salmonella Typhimurium DT104 or DT104) has been emerging as a common pathogen for human in Korea since 1997. In order to compare the genomic relationship and to search for the dominant strains in Korea, we conducted pulsed-field gel electrophoresis (PFGE) and IS200 fingerprinting of 25 epidemiological unrelated isolates from human and animals from Korea and cattle from America. Two Salmonella Typhimurium DT104 isolates from human in Korea and all 8 isolates from American cattle had indistinguishable patterns from the PFGE and IS200 fingerprinting but multidrug-resistant Salmonella Typhimurium isolates, including DT104, from Korean animals had diverse genetic patterns. The data suggest that a dominant DT104 strain might have circulated between Korean and American cattle and that it had a high level of clonality.