• 제어로봇시스템학회:학술대회논문집
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• 2005.06a
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• pp.151-157
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• 2005

In the chemical, mineral and electronics industries, mechanically stirred tanks are widely used for complex liquid and particle mixing processes. In order to understand the complex phenomena that occur in such tanks, it is necessary to investigate flow field in the vessel. Most difficulty on the numerical analysis of stirred tank flow field focused particularly on free surface analysis. In order to decrease the dead zone and improve the flow efficiency of a system with free surface, this paper presents a new method that overcomes free surface effects by properly combining the benefits of using experiment and 3-D CFD. This method is applied to study the mixing flow in an agitated tank. From the results of experimental studies using the PIV (particle image velocimetry) system, the distribution of mixing flow including free surface are obtained. And these values that are expressed as a velocity vector field have been patched for simulating the free surface. The results of velocity distribution obtained by 3-D CFD are compared with those of experimental results. The experimental data and the simulation results are in good agreement.

• KSBB Journal
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• v.14 no.5
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• pp.534-538
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• 1999

For the optimization of operating conditions for plant cell suspension culture in bioreactors, effects of bioreactor types, various kinds of impellers, and aeration rates were examined using Nicotiana tabacum cells as a model system. Stirred tank bioreactor and airlift bioreactor were used for the comparison of bioreactor type. Growth rates in both bioreactors were lower than in shake flasks. In terms of final cell concentration, stirred tank bioreactor supported a little bit better growth compared to airlift bioreactor. Impeller type did not affect cell growth significantly, but it was apparent that cell size index decreased in the case of using hollowed paddle impeller. When the aeration rate was maintained at 0.3 vvm, cell growth was the best. At above 1.0 vvm, growth inhibition as well a browning was noticed. In addition, it was found that cell size index reduced proportionally to the increased of aeration rate.

• 한국전산유체공학회:학술대회논문집
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• 2005.10a
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• pp.55-59
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• 2005

CFD analysis has been conducted to find the two stage impeller configuration which is the most suitable for a stirred tank with an internal helical cooling coil and a cooling jacket, which is frequently used in chemical industries for highly exothermic reactions ranged from low to medium viscosity. Two typical types of impellers are considered; pitched paddle impellers and Rushton turbine impellers. Interestingly, pitched paddle impellers show a good mixing performance for multi-species, whereas Rushton turbine impellers achieve a good mixing performance for multi-phases. Besides the type of an impeller, the location of an impeller is another important factor to be considered in order to accomplish an effective mixing. The best set of types and locations of two impellers is recommended based on the coefficient of variation(CoV) value and the heat removal capability obtained from CFD results. The former is a measure to quantify the degree of mixing.

• Biotechnology and Bioprocess Engineering:BBE
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• v.11 no.2
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• pp.164-167
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• 2006

The influence of temperature and agitation on the growth of Escherichia coli expressing hepatitis B core antigen (HBcAg) in stirred tank bioreactor were investigated. The highest specific growth rate for E. coli$(0.844 h^{-1})$ was achieved at a temperature of $37^{\circ}C$ and an agitation speed of 250 rpm. The activation energy for the growth of the E. coli strain W3110lQ in the stirred tank bioreactor was estimated to be 11 kcal/mol. The highest protein yield was achieved at a temperature of $44^{\circ}C$ and an agitation speed of 250 rpm. The relative protein concentration at $44^{\circ}C$ is 30 and 6% higher compared to that at 30 and $37^{\circ}C$, respectively.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2011.11a
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• pp.745-749
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• 2011

Two-dimemsional liquid-solid multiphase fluid dynamics was used to analyze the suspension and mix of liquid fuel and solid particles in fuel tank installed mixing impeller. In this paper, the multiphase flow was modeled using Eulerian Grandular Multiphase model. Experimental measurements of the axial distribution of solids concentration in stirred tanks under 12vol% solid loading were used for comparison with the CFD simulation. Four cases for the impeller location and flow pumping direction also were reviewed under 10.5% solids loading and 700rpm in fuel mix tank. The result of quality of suspension was compared with each cases and the impeller location and operation of mixing fuel tank was established.

• 유체기계공업학회:학술대회논문집
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• 2001.11a
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• pp.454-459
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• 2001

The present study is concerned with the flow patterns induced by other impellers in a rectangular tank Impellers are FBT(Flat blade turbine), PBT(Pitched blade turbine), Shroud turbine, Rushton Turbine, and Helical ribbon turbine. The solution of flows in moving reference frames requires the use of 'moving' cell zone. The moving zone approaches are MRF(Multiple reference frame), which is a steady-state approximation and Sliding method, which is a unsteady-state approximation. Numerical results using two moving zone approaches are compared with experiments by Ranade & Joshi, which have done extensive LDA measurements of the flow generated by a standard six-bladed Rushton turbine in a cylindrical baffled vessel. In this paper we simulated the flow patterns with above mentioned moving zone approaches and impellers. Turbulence model is RNG k-$\epsilon$ model.

• Journal of the Korean Chemical Society
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• v.6 no.2
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• pp.130-132
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• 1962

A new method of evaluating the mixing time in the continuous flow stirred tank is herein proposed. Experimental results to test the concept are also presented.The mixing time is defined as the time interval between the injection of a slug of an electrolyte solution into the tank and the moment at which an essentially straight line begins on a plot of the conductivity of effluent versus time.The proposed method of measuring the mixing time is valid even for the low mixing time (5 seconds) and the results obtained agree well with previous work, where the mixing time measurements were carried out by the injection of a dye into the feed stream.

• KSBB Journal
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• v.13 no.6
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• pp.700-705
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• 1998

Studies were made to investigate effects of the scale-up of stirred tank bioreactors on cell growth and alkaloids production for suspension cultures of Eschscholtzia californica. In the 1.5 L STR, cell lysis was observed at 110 rpm or higher agitation speed. The agitation speed of 30 L STR was 43.7 rpm to maintain the same shear stress developed in 1.5 L STR of 100 rpm. As a result of scale-up from 1.5 L to 30 L STR, the specific growth rate was decreased from 0.12 to 0.07 day-1. The alkaloids productivity was also decreased from 0.24 to 0.14 mg/L-day. Changes of mixing performance and oxygen transfer were studied to explain the decrease of cell growth and alkaloids production. Decreased oxygen transfer rate coefficient(KLa) and increased mixing time by the scale-up was observed at various aeration rates.

• KSBB Journal
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• v.14 no.4
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• pp.440-444
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• 1999

For the effecient production of clavulanic acid., a mutant strain Streptomyces clavuligerus KK was selected from Streptomyces clavuligerus ATCC 27064 through mutation with NTG. S. clavuligerus ATCC 27064 produced about 200 mg/L of calvulanic acid when the medium was composed of 1%(W/V) glycerol, 1.5%(W/V) soybean flour, 0.1%(W/V) $KH_2PO_4$, 0.2%(V/V) soybean oil. A selected mutant, S. clavuligerus KK, produced about 1150 mg/L of clavulanic acid in the same medium. After the addition of $MgSO_4$ to the basal medium, S. clavuligerus KK produced about 1550 mg/L of clavulanic acid, with shows about 1.3 times higher than that produced in the basal medium. In order to select the proper bioreactor for the production of clavulanic acid, a batch culture was performed in an airlift, a bubble column and an stirred tank bioreactors. In an airlift bioreactor, about 1350 mg/L of clavulanic acid was produced, in a bubble column bioreactor, about 1550 mg/L, in a stirred tank bioreactor, about 2200 mg/L, respectively. The production of clavulanic acid in stirred tank bioreactor was about 50% higer than that by an airlift and a bubble column bioreactors. According to this result, the stirred tank bioreactor was selected as a proper bioreactor.

• Proceedings of the KSME Conference
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• 2003.04a
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• pp.1806-1813
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• 2003

The stirred tank reactor is one of the most commonly used devices in industry for achieving mixing and reaction. Here we report on results obtained from the large eddy simulations of flow inside the tank performed using a spectral multi-domain technique. The computations were driven by specifying the impeller-induced flow at the blade tip radius. Stereoscopic PIV measurements (Hill et $al.^{(1)}$) along with the theoretical model of the impeller-induced flow (Yoon et $al.^{(2)}$) were used in defining the impeller-induced flow as superposition of circumferential, jet and tip vortex pair components. Large eddy simulation of flow in a stirred tank was carried out for the three different Reynolds numbers of 4000, 16000 and 64000. The effect of different Reynolds numbers is well observed in both instantaneous and time averaged flow fields. The instantaneous and mean vortex structures are identified by plotting an isosurfaces of swirling strength for all Reynolds numbers. The Reynolds number dependency of the nondimeansional eddy viscosity, resolve scale and subgrid scale dissipations is clearly shown in this study.