• Analytical Science and Technology
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• v.18 no.5
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• pp.444-451
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• 2005

The accurate and simple extraction method for sterols and fatty acids in sesame oil was developed. The new method improved the extraction efficiency of sesamin in sesame oil and the ratio of sesamin over campesterol. It will be applied to judgement of adulteration of plant edible oils. The minor components of sterols were also confirmed. The simultaneous determination of sterols and fatty acids with derivatization were processed, but it was not enough to confirm adulteration, thus need more experiments.

• Journal of Nutrition and Health
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• v.16 no.2
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• pp.107-114
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• 1983

There has been no specific test available for identifying the sesame oil among common edible oils. As the contents of sesamin and the ratio of sterols allowed the estimation for the genuine sesame oil, the author investigated to establish some instrumental methods for verification of genuine sesame oil and its distribution in the market. The sesame oil was saponified and the sesamin and sterols were isolated from the unsaponiable fraction by Florisil column chromatography. The individual components were determined by gas- chromatography and sesamin standard (purified sesamin) was obtained by silicagel column chromatography. The gas- chromatographic condition using Flame Ionization Detector supported on 10% OV-101 with di-(2-ethylhexyl) sebacate as an internal standard was suitable, and quantitation of sesamin and sterols, including campesterol, stigmasterol and ${\beta}-sitosterol$ was carried out. The results of this study showed that contents of sesamin in genuine sesame oil were 0.3-0.5% and the ratio of stigmasterol to compesterol was 0.3-0.6 and ${\beta}-sitosterol$ to campesterol 3.0-3.8. The 50 samples from the markets in Seoul were composed of 70% genuine sesame oil, and others were mixed with palm oil, rape seed oil and soybean oil.

• Korean Journal of Pharmacognosy
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• v.8 no.4
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• pp.149-153
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• 1977

Evodia daniellii $H_{EMSLEY}$ (Rutaceae) is cultivated in Korea and used as a folkmedicine for gastric inflammation, extermination of noxious insects, headache and antiinflammatory. The seed oil of this plant also has been used in various diseases, for example, dermatitis, scabies and so forth. Fructus Evodiae seed oil sterols were obtained from the nonsaponifiable matters of ethereal extract of the seed. The composition of sterols have been determined by gas liquid chromatography and thin layer chromatography on the preparative plates. It was noted that ${\beta}-sitosterol$ was the major sterols in the Evodia daniellii $H_{EMSLEY}$ in Korea. The results showed that contents of Evodia daniellii $H_{EMSLEY}$ sterols were campesterol 14.01%, stigmasterol 4.36%, ${\beta}-sitosterol$ 71.26%, ${\delta}^7-sterol$ 9.21% and unknown sterol 1.16% by method of triangulation and campesterol 13.02%, stigmasterol 3.87%, ${\beta}-sitosterol$ 74.34%, ${\delta}^7-sterol$ 8.67% and unknown sterol 1.85% by method of planimetry.

• YAKHAK HOEJI
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• v.41 no.5
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• pp.547-553
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• 1997

The purpose of this investigation was to determine the inhibition on $Na^+,\;K^+$-ATPase, cyclic AMP phosphodiesterase and platelet activation by marine sterols isolated from octocorals. Three marine polyhydroxysterols, 7${\alpha},\;8{\alpha}-epoxy-3b{\beta},\;5{\alpha},\;6{\alpha}-trihydroxycholestane (1),\;24-methyl-7{\alpha},\;8{\alpha}-epoxy-3{\beta},\;5{\alpha},\;6{\alpha}-trihydroxycholest-22-ene (2),\;and\;7{\alpha},\;8{\alpha}-epoxy-3{\beta},\;5{\alpha},\;6{\alpha}-trihydroxycholest-22-ene (3)$, were isolated from the Gorgonian Acabaria undulata. Five marine sterols(compound 4, 5, 6, 7, 8) were isolated from the soft coral Alcyonium gracillimum. Three marine polyhydroxysterols (1, 2, 3) and pregna-1. 20-diene-3-one (8) exhibit a potent inhibitory effect on rabbit platelet aggregation induced by collagen and thrombin. Those polyhydroxysterols also exhibit a potent inhibitory effect on cyclic AMP phosphodiesterase. Compound 6 with an unusual cyclic enolether exhibit a inhibitory effect on $Na^+,\;K^+$-ATPase.

• Korean Journal of Food Science and Technology
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• v.8 no.4
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• pp.201-206
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• 1976

Korean ginseng sterols were obtained from the non-saponifiable matters of etherial extract of the root. The composition of sterols have been determined by gas liquid chromatography and thin-layer chromatographic analysis. The results showed that contents of sterols were ${\beta}-sitosterol$, 77.87% by method of triangulation and 82.72% by methode of planimetry and stigmasterol,15.39% by methode of triangulation and 13.92% by methode of planimetry and campesterol, 6.74% by method of triangulation and 4.36% by methode of planimetry.

• Korean Journal of Food Science and Technology
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• v.14 no.2
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• pp.174-178
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• 1982

Various separation methods such as solvent extraction, chemical treatment and molecular distillation were tested for the separation of tocopherols and sterols from soy oil scum. The end products of these methods were tocopherol concentrates and sterol crystals. In the solvent extraction, purity and yield of tocopherols were 21.2% and 28.3%, and those of sterols were 69.2% and 2.6%. In the chemical treatment, purity and yield of tocopherols were 11.8% and 76.4% and those of sterols were 85.1% and 34.3% respectively. In the molecular distillation, purity and yield of tocopherols were 45.0% and 68.0%, and those of sterols were 49.3% and 57.0% respectively. The end products from the methods were characterized by HPLC. Based on the results of this study, the molecular distillation method was found to be more efficient than any other method tested.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.9 no.3
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• pp.203-208
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• 1976

The composition of fatty acids and sterols in the muscle lipid of eel, Anguilla japonicus Temminck & Schlegel was investigated quantitatively by using gas liquid chromatography(GLC) and the sterols separated from unsaponifiable matter by thin-layer chromatography were identified by GLC, infrared spectroscopy, nuclear magnetic resonance and GLC-mass spectrometry respectively. The fatty acids of the muscle lipid consisted of the large amount of C18:1 $(40.98\%)$, $C16:0(23.71\%)$, $C16:1(13.37\%)$ acids, and small amount of $C14:0(7.19\%),\;C18:0(5.55\%),\;C20:1(1.83\%),\;18:2(1.42\%)$ acids, and $C18:3(0.92\%),\;C15:0(0.73\%),\;C14:1(0.50\%)$ acids were smaller, Choleterol$(96.58\%)$ was a major component of the sterol fraction and 24-methylene cholesterol $(3.42\%)$ was also detected as a minor component.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.37 no.3
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• pp.74-80
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• 2005

The compositions of residual extractives in woodmeal, unbleached and oxygen-delignified aspen kraft pulps were investigated with gas chromatography(GC) and gas chromatography-mass spectrometry (GC-MS) with focus on fate of extractives in kraft pulping and oxygen delignification. Steryl esters and shorter retention time (shorter than palmitic acid) extractives were main extractives in aspen woodmeal. Shorter retention time extractives were well removed in kraft pulping. Sterol esters were hydrolyzed to sterols and fatty acids. Sterols and fatty acids were two major extractives classes in unbleached kraft pulps. Linoleic acid was main fatty acids in unbleached pulps compared with palmitic acid which is generally found in aspen woodmeal. Sterolsand fatty acids were also two major extractives classes in oxygen-delignified kraft pulps. However, linoleic acid was well removed in oxygen delignification.

• Journal of Applied Biological Chemistry
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• v.54 no.4
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• pp.309-312
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• 2011

Chipped stem wood from Lindera glauca was extracted repeatedly with 80% aqueous methanol at room temperature, and the concentrated methanolic extract was successively partitioned with ethyl acetate (EtOAc), n-butyl alcohol, and $H_2O$. From the EtOAc fraction, four sterols were isolated through a repeated silica gel and octadecyl silica gel column chromatography. The chemical structures of the sterols were elucidated as ${\beta}$-sitosterol (1), 7-ketositosterol (2), 7${\beta}$-hydroxysitosterol (3), and daucosterol (4). Among them, compounds 2 and 3 were isolated for the first time from the stem woods of this plant.

• Journal of the Korean Wood Science and Technology
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• v.34 no.5
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• pp.104-110
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• 2006

We investigated why aspen extractives are more resistant in kraft pulping than pine extractives. Residual extractives content in aspen kraft pulps were 0.5~1.1% compared with 0.1~0.2% in pine pulps. This different response arises from the different composition of extractives in wood chips. Resin acids in pine were almost completely removed in kraft pulping but those are not existence in aspen. Slower saponification of aspen steryl esters resulted from different chemical structure of aspen steryl esters. Main sterols in aspen steryl esters were 24-methyl cyclolanostenol which was highly resistant to alkaline hydrolysis with its characteristic steric hindrance. Sterols in aspen were not well removed in kraft pulping. The relative composition of sterol in aspen kraft pulps was increased with increasing pulping time. The presence of fatty acids in aspen kraft pulps is considered to unusual. Fatty acids in alkaline are supposed to be well ionized and removed well in the washing stage. Nevertheless, there were significant amount of fatty acids remaining in aspen kraft pulps.