• 분석과학
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• 제18권5호
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• pp.444-451
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• 2005

참기름에서 스테롤류와 지방산을 간편하고 정확하게 추출하는 방법을 개발하였다. 새로운 방법은 특히 세사민의 추출 효율을 크게 향상하여 세사민과 캄파스테롤의 비율을 크게 향상시켜 감정의 도구로 응용이 가능할 것으로 판단되었다. 또 기타 스테롤류도 확인하였다. 유도체화를 진행시키면 지방산과 스테롤류의 동시 분석이 가능하였으나, 감정의 도구로는 더 많은 실험이 필요할 것으로 판단되었다.

• Journal of Nutrition and Health
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• 제16권2호
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• pp.107-114
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• 1983

There has been no specific test available for identifying the sesame oil among common edible oils. As the contents of sesamin and the ratio of sterols allowed the estimation for the genuine sesame oil, the author investigated to establish some instrumental methods for verification of genuine sesame oil and its distribution in the market. The sesame oil was saponified and the sesamin and sterols were isolated from the unsaponiable fraction by Florisil column chromatography. The individual components were determined by gas- chromatography and sesamin standard (purified sesamin) was obtained by silicagel column chromatography. The gas- chromatographic condition using Flame Ionization Detector supported on 10% OV-101 with di-(2-ethylhexyl) sebacate as an internal standard was suitable, and quantitation of sesamin and sterols, including campesterol, stigmasterol and ${\beta}-sitosterol$ was carried out. The results of this study showed that contents of sesamin in genuine sesame oil were 0.3-0.5% and the ratio of stigmasterol to compesterol was 0.3-0.6 and ${\beta}-sitosterol$ to campesterol 3.0-3.8. The 50 samples from the markets in Seoul were composed of 70% genuine sesame oil, and others were mixed with palm oil, rape seed oil and soybean oil.

• 생약학회지
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• 제8권4호
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• pp.149-153
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• 1977

Evodia daniellii $H_{EMSLEY}$ (Rutaceae) is cultivated in Korea and used as a folkmedicine for gastric inflammation, extermination of noxious insects, headache and antiinflammatory. The seed oil of this plant also has been used in various diseases, for example, dermatitis, scabies and so forth. Fructus Evodiae seed oil sterols were obtained from the nonsaponifiable matters of ethereal extract of the seed. The composition of sterols have been determined by gas liquid chromatography and thin layer chromatography on the preparative plates. It was noted that ${\beta}-sitosterol$ was the major sterols in the Evodia daniellii $H_{EMSLEY}$ in Korea. The results showed that contents of Evodia daniellii $H_{EMSLEY}$ sterols were campesterol 14.01%, stigmasterol 4.36%, ${\beta}-sitosterol$ 71.26%, ${\delta}^7-sterol$ 9.21% and unknown sterol 1.16% by method of triangulation and campesterol 13.02%, stigmasterol 3.87%, ${\beta}-sitosterol$ 74.34%, ${\delta}^7-sterol$ 8.67% and unknown sterol 1.85% by method of planimetry.

• 약학회지
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• 제41권5호
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• pp.547-553
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• 1997

The purpose of this investigation was to determine the inhibition on $Na^+,\;K^+$-ATPase, cyclic AMP phosphodiesterase and platelet activation by marine sterols isolated from octocorals. Three marine polyhydroxysterols, 7${\alpha},\;8{\alpha}-epoxy-3b{\beta},\;5{\alpha},\;6{\alpha}-trihydroxycholestane (1),\;24-methyl-7{\alpha},\;8{\alpha}-epoxy-3{\beta},\;5{\alpha},\;6{\alpha}-trihydroxycholest-22-ene (2),\;and\;7{\alpha},\;8{\alpha}-epoxy-3{\beta},\;5{\alpha},\;6{\alpha}-trihydroxycholest-22-ene (3)$, were isolated from the Gorgonian Acabaria undulata. Five marine sterols(compound 4, 5, 6, 7, 8) were isolated from the soft coral Alcyonium gracillimum. Three marine polyhydroxysterols (1, 2, 3) and pregna-1. 20-diene-3-one (8) exhibit a potent inhibitory effect on rabbit platelet aggregation induced by collagen and thrombin. Those polyhydroxysterols also exhibit a potent inhibitory effect on cyclic AMP phosphodiesterase. Compound 6 with an unusual cyclic enolether exhibit a inhibitory effect on $Na^+,\;K^+$-ATPase.

• 한국식품과학회지
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• 제8권4호
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• pp.201-206
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• 1976

한국인삼(韓國人蔘)의 뿌리중(中)의 ether가용성분중(可溶成分中)에서 불령화물(不齡化物)을 검출(檢出)한다음에 Thinlayer chomatography법(法)과 Gas liguid Chromatography법(法)에 의해서 Sterol의 성분(成分)을 검출(檢出)하였다. 그 결과인삼중(結果人蔘中)에서 stigmasterol이 반치폭법(半値幅法)에 의하면 15.39%. 그리고 planimetry법(法)에 의하면 13.92%이었고 ${\beta}-Sitosterol$은 반치폭법(半値幅法)에 의하면 77.87% 그리고 planimetry법(法)에 의하면 82.72%로 가장 함량(含量)이 높았고 Campesterol은 반치폭법(半値幅法)에서는 6.74% 그리고 Planimetry법(法)에서는 4.36%가 됨을 알수 있다.

• 한국식품과학회지
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• 제14권2호
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• pp.174-178
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• 1982

대두유의 탈취 증류분으로 부터 토코폐롤과 스테롤을 분리해내기 위하여 분자 증류법, 용매추출법, 화학처리법 등을 이용하였으며 최종 산물로 토코폐롤 농축물과 스테롤 결정을 얻었다. 용매추출법의 경우, 얻어진 토코폐롤의 순도와 회수율은 각각 21.2%와 28.3%였으며 스테롤의 순도와 회수율은 각각 69.2%와 2.6%였다. 화학처리법의 경우, 토코폐롤의 순도와 회수율은 11.8%와 76.4%였으며 스테롤의 순도와 회수율은 85.1%와 34.3%였다. 한편 분자증류법의 경우 토코폐롤의 순도와 회수율은 45%와 68%였으며 스테롤의 순도와 회수율은 각각 49.3% 57%로 나타났다. 또한 얻어진 토코폐롤 농축물을 HPLC로 비교하였다. 이 연구의 결과로 분자증류법이 실험한 다른 방법보다 더 효과적임을 알았다.

• 한국수산과학회지
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• 제9권3호
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• pp.203-208
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• 1976

뱀장어 근육유의 지방질 및 sterol 조성을 GLC로 분석하였고 특히 sterols조성을 IR, NMR, GLC-MS로 동정하여 다음과 같은 결과를 얻었다. 1) 뱀장어 근육유의 지방산 조성은 $C_{18:1}(40.98\%),\;C_{16:0}(23.71\%),\;C_{16:1}(13.37\%)$산이 가장 높은 비율로 함유하며, 다음은 $C_{14:0}(7.19\%),\; C_{18:0}(5.55\%),\;C_{20:1}(1.83\%),\;C_{18:2}(1.42\%)$산 이였고, $C_{18:3}(0.92\%),\;C_{15:0}(0.73\%),\;C_{14:1}(0.50\%)$산은 그 함량이 가장 적었다. 2) sterols 조성은 cholesterol$(96.58\%)$이 대부분이 였고, 24-methylene cholesterol$(3.43\%)$이 있음을 알았다.

• 펄프종이기술
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• 제37권3호
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• pp.74-80
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• 2005

The compositions of residual extractives in woodmeal, unbleached and oxygen-delignified aspen kraft pulps were investigated with gas chromatography(GC) and gas chromatography-mass spectrometry (GC-MS) with focus on fate of extractives in kraft pulping and oxygen delignification. Steryl esters and shorter retention time (shorter than palmitic acid) extractives were main extractives in aspen woodmeal. Shorter retention time extractives were well removed in kraft pulping. Sterol esters were hydrolyzed to sterols and fatty acids. Sterols and fatty acids were two major extractives classes in unbleached kraft pulps. Linoleic acid was main fatty acids in unbleached pulps compared with palmitic acid which is generally found in aspen woodmeal. Sterolsand fatty acids were also two major extractives classes in oxygen-delignified kraft pulps. However, linoleic acid was well removed in oxygen delignification.

• Journal of Applied Biological Chemistry
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• 제54권4호
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• pp.309-312
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• 2011

Chipped stem wood from Lindera glauca was extracted repeatedly with 80% aqueous methanol at room temperature, and the concentrated methanolic extract was successively partitioned with ethyl acetate (EtOAc), n-butyl alcohol, and $H_2O$. From the EtOAc fraction, four sterols were isolated through a repeated silica gel and octadecyl silica gel column chromatography. The chemical structures of the sterols were elucidated as ${\beta}$-sitosterol (1), 7-ketositosterol (2), 7${\beta}$-hydroxysitosterol (3), and daucosterol (4). Among them, compounds 2 and 3 were isolated for the first time from the stem woods of this plant.

• Journal of the Korean Wood Science and Technology
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• 제34권5호
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• pp.104-110
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• 2006

We investigated why aspen extractives are more resistant in kraft pulping than pine extractives. Residual extractives content in aspen kraft pulps were 0.5~1.1% compared with 0.1~0.2% in pine pulps. This different response arises from the different composition of extractives in wood chips. Resin acids in pine were almost completely removed in kraft pulping but those are not existence in aspen. Slower saponification of aspen steryl esters resulted from different chemical structure of aspen steryl esters. Main sterols in aspen steryl esters were 24-methyl cyclolanostenol which was highly resistant to alkaline hydrolysis with its characteristic steric hindrance. Sterols in aspen were not well removed in kraft pulping. The relative composition of sterol in aspen kraft pulps was increased with increasing pulping time. The presence of fatty acids in aspen kraft pulps is considered to unusual. Fatty acids in alkaline are supposed to be well ionized and removed well in the washing stage. Nevertheless, there were significant amount of fatty acids remaining in aspen kraft pulps.